170,715 results
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Plasmid#59026PurposeTALEN designed to target the human AAVS1/PPP1R12C locusDepositorInsertAAVS1-TALEN-R
UseAAV and TALENTags3xFlag and NLSExpressionMammalianPromoterCMVAvailable SinceAug. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRRLsin-SV40 T antigen-IRES-mCherry
Plasmid#58993Purposelentiviral expression of SV40 large T antigen and mCherry red fluorescent reporterDepositorInsertsCMV enhancer-chicken beta actin promoter
SV40 large T antigen
IRES-mCherry red fluorescent protein
UseLentiviralExpressionMammalianMutationEcoRV site in MCS converted to BamHI sitePromoterCMV enhancer-chicken beta actinAvailable SinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPspCas13b-3xEGFP-NLS-3xFlag
Plasmid#132398Purposeoverexpression in human cellsDepositorInsertdPspCas13b
UseLentiviralTagsflagExpressionMammalianMutationH133A; H1059AAvailable SinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-BRD2
Plasmid#65376PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorAvailable SinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV mCherry-GFP-LC3B WT
Plasmid#123230PurposeExpresses mCherry-EGFP-LC3B wild-type in mammalian cells. Fluorescent tandem reporter for autophagosomes. High expression driven by CMV promoter.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
TagsEGFP and mCherryExpressionMammalianPromoterCMVAvailable SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV_AncBE4max_P2A_GFP
Plasmid#112100PurposeC:G-to-T:A base editingDepositorInsertAncBE4max_P2A_GFP
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG- gate-pGK-HYG
Plasmid#107397PurposeGateway destination vectorDepositorTypeEmpty backboneUseLentiviral; Gateway destinationTagsFLAGExpressionMammalianAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
SigmaR1-mNeonGreen
Plasmid#226566PurposeEncodes SigmaR1 protein labeled with mNeonGreenDepositorAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP-GFP-LC3-RFP
Plasmid#84573PurposeExpresses GFP-LC3-RFP in mammalian cells to measure autophagic fluxDepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseRetroviralTagsEGFP and mRFP1ExpressionMammalianAvailable SinceJan. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV-CMV-LoxP-DsRed-LoxP-eGFP
Plasmid#65726PurposeFunctions as a genetic Cre reporter that switches from DsRed expression to eGFP expression upon presence of Cre recombinase.DepositorInsertLoxP-DsRed-LoxP-eGFP
UseCre/Lox and LentiviralExpressionMammalianPromoterCMVAvailable SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
cyto-iATPSnFR1.0
Plasmid#102550Purposecytoplasmic ATP sensor for mammalian cellsDepositorInsertiATPSnFR1.0
ExpressionMammalianAvailable SinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 neo
Plasmid#13425Purpose3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses neomycin for selection.DepositorInsertnon-hairpin 18bp
UseLentiviral and RNAiExpressionMammalianPromoterU6 promoterAvailable SinceDec. 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMCB306-GFP-mito
Plasmid#229232PurposeExpresses GFP localized to mitochondriaDepositorAvailable SinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-DeAct-GS1
Plasmid#89445PurposeExpresses EGFP-GS1 (Human Gelsolin 53-176) in mammalian cellsDepositorInsertDeAct-GS1 (GSN Human)
TagsEGFPExpressionMammalianMutationTruncation spanning cytoplasmic Gsn amino acids 5…PromoterCMVAvailable SinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
TDP-REGv2(mScarlet-FLAG)#7 in pTwist-CMV backbone
Plasmid#216152PurposeExpresses mScarlet in cells with TDP-43 loss-of-function. This plasmid has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells. It uses a cryptic exon. [Code 'A11'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor CommentsDepositorInsertmScarlet with cryptic exon and C-terminal FLAG
ExpressionMammalianPromoterCMVAvailable SinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Fab_8D3_2_H-H6
Plasmid#176075PurposeExpress His6 tagged heavy chain of Fab_8D3_2 in mammalian cellsDepositorInsertHis6 tagged heavy chain of Fab_8D3_2
TagsHis tagExpressionMammalianPromoterCAG promoterAvailable SinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Fab_8D3_2_L
Plasmid#176076PurposeExpress light chain of Fab_8D3_2 in mammalian cellsDepositorInsertLight chain of Fab_8D3_2
ExpressionMammalianPromoterCAG promoterAvailable SinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(3,4,5)P3 Probe
Plasmid#211514PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-3,4,5-Trisphosphate (PI(3,4,5)P3) from bacteriaDepositorInsertBTK1-PH
Tags6xhis-SNAPExpressionBacterialAvailable SinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW-Cdt1-mCherry-Puro
Plasmid#193763PurposeDoxycyline-inducible (TetOn) human Cdt1 (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)DepositorInsertCdt1-mCherry (CDT1 Human)
UseLentiviralTagsWTMutationhuman Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…PromoterTREAvailable SinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF1alpha-pcDNA3
Plasmid#18949DepositorInsertHypoxia inducible factor 1 alpha (HIF1A Human)
TagsHA-tagExpressionMammalianMutationwt cDNAAvailable SinceSept. 3, 2008AvailabilityAcademic Institutions and Nonprofits only