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pRSI9-U6-(sh)-UbiC-TagRFP-2A-Puro
(Plasmid #28289)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 28289 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRSI9-U6-(sh)-UbiC-TagRFP-2A-Puro
  • Backbone manufacturer
    Cellecta
  • Backbone size (bp) 7500
  • Vector type
    Mammalian Expression, Lentiviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    SURE
  • Growth instructions
    Plasmids need to be grown in OmniMAX 2 T1R (Life Technologies) or SURE (Agilent) Recombination defective E.coli cells. SURE cells are kanamycin and tetracycline resistant. When transforming the SURE strain with plasmids carrying chloramphenicol resistance gene, select for transformants on LB agar plates containing 100 µg/ml chloramphenicol. The SURE strain is resistant (Camr) to concentrations of <40 µg/ml chloramphenicol, but sensitive (Cams) to 100 µg/ml chloramphenicol.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    None
  • Alt name
    DECIPHER shRNA Expression Vector

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (BpiI) (not destroyed)
  • 3′ cloning site BbsI (BpiI) (not destroyed)
  • 5′ sequencing primer FwdU6 (CAAGGCTGTTAGAGAGATAATTGGAA)
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSI9-U6-(sh)-UbiC-TagRFP-2A-Puro was a gift from Alex Chenchik & Gus Frangou (Addgene plasmid # 28289)