Thy1 promoter construct
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||20736||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Gene/Insert nameThy1 promoter
SpeciesM. musculus (mouse)
Insert Size (bp)6500
Entrez GeneThy1 (a.k.a. CD90, T25, Thy-1, Thy-1.2, Thy1.1, Thy1.2)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site PvuI (unknown if destroyed)
- 5′ sequencing primer Thy1F1 (TCTGAGTGGCAAAGGACCTTAGG)
- 3′ sequencing primer n/a (Common Sequencing Primers)
Contains 6.5 kb of the murine thy1.2 gene, extending from the promoter to the intron following exon 4, but lacking exon 3 and its flanking introns (see Figure 1 in Caroni 1997).
P. Caroni, Overexpression of growth-associated proteins in the neurons of adult transgenic mice. J. Neurosci. Methods 71 (1997), pp. 3–9 https://www.ncbi.nlm.nih.gov/pubmed/9125370
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Thy1 promoter construct was a gift from Joshua Sanes (Addgene plasmid # 20736 ; http://n2t.net/addgene:20736 ; RRID:Addgene_20736)
For your References section:Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. Feng G, Mellor RH, Bernstein M, Keller-Peck C, Nguyen QT, Wallace M, Nerbonne JM, Lichtman JW, Sanes JR. Neuron. 2000 Oct . 28(1):41-51. 10.1016/S0896-6273(00)00084-2 PubMed 11086982