159,428 results

1. pPSU1

   Plasmid

   #89439

   Purpose

   High copy number plasmid 1 to prepare Penn State DNA molecular weight ladders

   Depositor

   Song Tan

   Article

   Henrici et al Sci Rep. 2017 May 26;7(1):2438. doi: 10.

   Inserts

   500 bp EcoRV fragment
   1000 bp EcoRV fragment
   1500 bp EcoRV fragment
   2000 bp EcoRV fragment
   500 bp PstI fragment
   700 bp PstI fragment
   800 bp PstI fragment (EHMT2 Human)
   900 bp PstI fragment
   1000 bp PstI fragment (BMI1 Human)
   2000 bp PstI fragment
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSNAPf-Cox8A Control Plasmid

   Plasmid

   #101129

   Purpose

   Expression of SNAP-Tagged Cox8A in Mammalian cells

   Depositor

   New England Biolabs, Ana Egana

   Insert

   Cox8A (COX8A Human)
   

   Use

   Tags

   SNAP-tag (SNAPf)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 27, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pcDNA3.1 TaGu N-1F

   Plasmid

   #203762

   Purpose

   For expression of N-flag tagged TaGu R2 protein in mammalian cells

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   R2 retroelement protein
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-RAB-ICUE

   Plasmid

   #118480

   Purpose

   ddRFP-based single-color biosensor for monitoring cAMP dynamics.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   RAB-ICUE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. Polymerase expression - pCMV-T7-EcKlenow (LM2692)

   Plasmid

   #208963

   Purpose

   Unfused EcKlenow DNA polymerase (-exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   EcKlenow-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHMM

   Plasmid

   #67717

   Purpose

   Expression vector MBP/hexahistidine tagged variant of the MS2 coat protein

   Depositor

   Jeffrey Kieft, Robert Batey

   Article

   Batey et al RNA. 2007 Aug;13(8):1384-9. Epub 2007 Ju

   Insert

   Hexahistidine/Maltose binding protein/MS2 coat protein fusion
   

   Use

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 RNA polymerase

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp

   Plasmid

   #125228

   Purpose

   Expresses SPARK2 NanoLuc-βarrestin2-TEVp in mammalian cells

   Depositor

   Alice Ting

   Article

   Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

   Insert

   NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
   

   Use

   AAV

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTargetF

   Plasmid

   #62226

   Purpose

   Constitutive expression of sgRNA without donor editing template DNA

   Depositor

   Sheng Yang

   Article

   Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

   Insert

   sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pij23119

   Available since

   March 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPSU2

   Plasmid

   #89566

   Purpose

   High copy number plasmid 2 to prepare Penn State DNA molecular weight ladders

   Depositor

   Song Tan

   Article

   Henrici et al Sci Rep. 2017 May 26;7(1):2438. doi: 10.

   Inserts

   750 bp EcoRV fragment
   3000 bp EcoRV fragment
   4000 bp EcoRV fragment
   50 bp PstI fragment
   100 bp PstI fragment
   200 bp PstI fragment
   300 bp PstI fragment
   400 bp PstI fragment
   500 bp PstI fragment
   600 bp PstI fragment
   1500 bp Psti fragment
   4100 bp PstI fragment
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. HLA_A0101

    Plasmid

    #165009

    Purpose

    Expression of HLA-A*01:01 cDNA

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    HLA-A*01:01 (HLA-A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. NLS-PyronicSF/pCMV-myc-nuc

    Plasmid

    #124814

    Purpose

    Highly resposive GFP-based pyruvate nanosensor with a nuclear localization signal.

    Depositor

    Luis Felipe Barros

    Article

    Arce-Molina et al eLife. 2020 Mar 6;9. pii: 53917. doi: 10

    Insert

    PyronicSF
    

    Use

    Tags

    Nuclear Localization Signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFP.E227

    Plasmid

    #138249

    Purpose

    Reporter plasmid - Expresses three fluorescent reporters from three different ECF promoters

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    mCherry
    Terminator
    mTagBFP
    Terminator
    gfp
    Terminator
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    P17_up1691, P20_992, and iP16_3622

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-mCherry-CAAX

    Plasmid

    #129285

    Purpose

    Lentiviral plasmid for expression of fluorescent reporter mCherry targeted to the plasma membrane via CAAX polybasic sequence

    Depositor

    Carlos Carmona-Fontaine

    Article

    Janska et al Dis Model Mech. 2021 Aug 1;14(8). pii: 2

    Insert

    Fluorescent reporter mCherry fused to CAAX polybasic sequence
    

    Use

    Lentiviral

    Tags

    CAAX

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5-FRT/TO-3*Flag-APEX2 C-term PML

    Plasmid

    #187583

    Purpose

    Express FLAG epitope and APEX2-tagged PML fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    PML (PML Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEB1-mGFPmut2

    Plasmid

    #103980

    Purpose

    Plasmid encoding mGFPmut2

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mGFPmut2
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (S65A, V68L, …

    Promoter

    proC

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hsyn-GRAB_HA1h

    Plasmid

    #190473

    Purpose

    Express the high-affinity histamine sensor GRAB_HA1h in neurons

    Depositor

    Yulong Li

    Article

    Dong et al Neuron. 2023 Mar 7:S0896-6273(23)00128-9

    Insert

    Histamine sensor GRAB_HA1h (high affinity)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLX304-GW-DCK*-IRES-GFP

    Plasmid

    #176291

    Purpose

    Gateway vector for use in generating POI-DCK* fusion reporter

    Depositor

    William Kaelin

    Article

    Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

    Insert

    Mutant Deoxycytidine Kinase (DCK*, S74E R104M D133A) (DCK Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    S74E R104M D133A

    Promoter

    CMV

    Available since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Bxb1-GA_AIO-GZV-SynZiFTR_mNgn2 donor

    Plasmid

    #229789

    Purpose

    Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible expression of mouse neurogenin 2

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mNgn2; ZF10-NS3-p65 (Neurog2 Mouse)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ZF10 and CAG

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA-R-iLACCO1

    Plasmid

    #208025

    Purpose

    Biosensor for intracellular L-lactate

    Depositor

    Robert Campbell

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    R-iLACCO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDEST-cDNA5-FRT/TO-3*Flag-APEX2 C-term

    Plasmid

    #182926

    Purpose

    Express FLAG epitope and APEX2-tagged fusion protein in mammalian cells.

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Type

    Empty backbone

    Use

    Tags

    APEX2 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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