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PCNA directs type 2 RNase H activity on DNA replication and repair substrates.
Bubeck D, Reijns MA, Graham SC, Astell KR, Jones EY, Jackson AP
Nucleic Acids Res. 2011 May;39(9):3652-66. doi: 10.1093/nar/gkq980. Epub 2011 Jan 17.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
108695pGEX6P1‐Afu‐rnhB D101NFor expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with D101N catalytic site mutation
108696pGEX6P1‐Afu‐rnhBΔPIPFor expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with C-terminal PIP box deletion
108697pEGFP‐RNASEH2BFor expression of N-terminally eGFP-tagged human RNASEH2B in mammalian cells
108698pEGFP‐RNASEH2CFor expression of N-terminally eGFP-tagged human RNASEH2C in mammalian cells
108699pEGFP‐RNASEH1For expression of C-terminally eGFP-tagged human RNASEH1 (nuclear isoform) in mammalian cells
108700pEGFP‐RNASEH2AFor expression of N-terminally eGFP-tagged human RNASEH2A in mammalian cells

Antibodies from Article