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pcDNA5/FRT/TO_[Cox8a]x2_HaloTag9

Plasmid

#175539

Purpose

Expression of HaloTag9 at the inner membrane of mitochondria in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Cox8a-HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pHB-4

Plasmid

#140957

Purpose

For PHB production in E. coli (thrC3-pAC). Please note that this is listed as #140956 in the publication.

Depositor

Kang Zhou

Article

Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.

Insert

phaCAB

Expression

Bacterial

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-hChR2(H134R)-EYFP-WPRE

Plasmid

#20942

Purpose

3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by EF1a promoter for optogenetic activation

Depositor

Karl Deisseroth

Article

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Insert

channelrhodopsin-2

Use

Lentiviral

Expression

Mammalian

Mutation

H134R mutation in ChR2

Promoter

EF1alpha

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pMSCV(gfp)U6sgRNA5(BbsI)-PGKpuroBFP

Plasmid

#102798

Purpose

Retrovirus for delivery of one sgRNA (self-targeting) - GFP-targeting control plasmid contains GFP instead of PuroR

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Inserts

sgRNA
EGFP

Use

CRISPR and Retroviral

Expression

Mammalian

Mutation

H231L

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-Lifeact-7

Plasmid

#54602

Purpose

Localization: Actin, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

Lifeact-mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pET3a-hUBA1

Plasmid

#63571

Purpose

Expression of human UBA1 tagged with His

Depositor

Titia Sixma

Article

Pichler et al Nat Struct Mol Biol. 2005 Mar;12(3):264-

Insert

UBA1 (UBA1 Human)

Expression

Bacterial

Mutation

Isoform 2 (1018 amino acids)

Promoter

T7

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-eGFP-2A-mCherry_D4H

Plasmid

#194875

Purpose

Ratiometric D4H sensor for in vivo neuron-specific cholesterol visualization

Depositor

Peter Scheiffele

Article

Czernecki et al bioRxiv 2022.11.16.516849

Insert

GFP-T2A-mCherry-D4H

Use

AAV

Expression

Mammalian

Promoter

human Synapsin

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Neo

Plasmid

#139449

Purpose

Lentiviral vector with gRNA scaffold and neomycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

no sgRNA inserted; resistance gene: neoR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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PER2:Luc Plasmid

Plasmid

#206875

Purpose

Per2 promoter luciferase reporter

Depositor

Michelle Farkas

Article

Lin et al Integr Cancer Ther. 2019 Jan-Dec;18:1534

Insert

Period 2 (Per2 Mouse)

Use

Lentiviral and Luciferase

Expression

Mammalian

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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Cytoplasmic PancACe

Plasmid

#215708

Purpose

Expresses acetyl-CoA biosensor in the cytoplasm

Depositor

Katharine Diehl

Article

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

PancACe

Use

Lentiviral

Tags

FLAG, HA, and cytoplasmic localization sequence

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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mNeonGreen

Plasmid

#189976

Purpose

Part 3-4-5 plasmid to introduce mNeonGreen into circRNAs

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

mNeonGreen

Use

Cloning

Promoter

N/A

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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Ubiquitin G76C

Plasmid

#86583

Purpose

Bacterial expression of Ubiquitin G76C for cross linking studies

Depositor

Sharon Rozovsky

Article

Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

Insert

Ubiquitin (UBC Human)

Tags

His6-MBP

Expression

Bacterial

Mutation

TEV site; G76C

Promoter

Tac

Available Since

Feb. 24, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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L4552 x6NFATRE (NFAT) Ben DsRed-Express2 in TUPV1

Plasmid

#244201

Purpose

Expression of a DsRed-Express2 reporter under an NFAT-responsive promoter

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

NFAT-responsive promoter (6 NFAT binding sites + YB_TATA), dsRed-Express2 reporter

Use

Synthetic Biology

Expression

Mammalian

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pPEgRNA

Plasmid

#172716

Purpose

Delivery of guide RNA for prime editing

Depositor

Tilmann Weber

Article

Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

Insert

gRNA

Expression

Bacterial

Available Since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

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HaloTag-Ubiquitin Fusion Vector

Plasmid

#236943

Purpose

Express HaloTag(R)-Ubiquitin Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

Ubiquitin (UBC Human)

Tags

HaloTag (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV_AncBE4max

Plasmid

#112094

Purpose

C:G-to-T:A base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Insert

AncBE4max

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-Drosha

Plasmid

#62520

Purpose

wild-type human Drosha cDNA is subcloned into pEGFP-C1 vector between HindIII and KpnI sites

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha full length (DROSHA Human)

Tags

GFP

Expression

Mammalian

Available Since

March 20, 2015

Availability

Academic Institutions and Nonprofits only

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pMSCV(W-)U6sgRNA5(BbsI)-PGKpuroBFP

Plasmid

#102797

Purpose

Retrovirus for testing CRISPR KO activity (empty) - non-targeting control plasmid contains GFP instead of PuroR

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Inserts

sgRNA
EGFP

Use

CRISPR and Retroviral

Expression

Mammalian

Mutation

H231L

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pDY32 AAVS1-PuroR-9xTet0-minCMV-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

Plasmid

#161928

Purpose

Donor plasmid for integrating minCMV promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

Insert

Surface marker and Citrine activation reporter

Use

Synthetic Biology

Expression

Mammalian

Promoter

pEF

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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circRNA-synIRES-R25-mNeonGreen

Plasmid

#188115

Purpose

Template plasmid to transcribe circular RNA encoding mNeonGreen

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

mNeonGreen

Expression

Mammalian

Promoter

T7

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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