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pmCherry-C1-RanQ69L
(Plasmid #30309)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 30309 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pmCherry-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RanQ69L
  • Alt name
    RAs-related Nuclear protein Q69L
  • Alt name
    Ran
  • Species
    N/A
  • Insert Size (bp)
    650
  • Mutation
    Glutamine 69 is changed to Leucine
  • GenBank ID
    NP_006316
  • Entrez Gene
    RAN (a.k.a. ARA24, Gsp1, TC4)
  • Tag / Fusion Protein
    • FLAG-Cherry (N terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Andrew Wilde (University of Toronto)
  • Articles Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmCherry-C1-RanQ69L was a gift from Jay Brenman (Addgene plasmid # 30309 ; http://n2t.net/addgene:30309 ; RRID:Addgene_30309)
  • For your References section:

    Identification of a nuclear export signal in the catalytic subunit of AMP-activated protein kinase. Kazgan N, Williams T, Forsberg LJ, Brenman JE. Mol Biol Cell. 2010 Oct 1. 21(19):3433-42. 10.1091/mbc.E10-04-0347 PubMed 20685962