We narrowed to 5,654 results for: pCas

1. pSpCas9-2A-Puro-TBK1-gRNA (PX459)

   Plasmid

   #221550

   Purpose

   Expresses Cas9 and gRNA for disruption of TBK1 gene in human cells

   Depositor

   Shawn Ferguson

   Article

   Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

   Insert

   gRNA targeting human TBK1 (TBK1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   u6

   Available since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-mCherry-FKBP-GEF(ARHGEF1)

   Plasmid

   #85152

   Purpose

   acute activation of Rho through rapamycin-induced translocation to membrane-localized FRB

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Insert

   Fusion of mCherry, FKBP, and the DH-domain of ARHGEF1 (amino acids 380-630) (ARHGEF1 Human)
   

   Use

   Tags

   mCherry-FKBP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-mCherry-FKBP-GEF(TIAM1)

   Plasmid

   #85156

   Purpose

   acute activation of Rac through rapamycin-induced translocation to membrane-localized FRB

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Insert

   Fusion of mCherry, FKBP, and the DH-domain of human TIAM1 (amino acids 1012-1250)
   

   Use

   Tags

   mCherry-FKBP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV pCAG-FLEX-mScarlet-WPRE

   Plasmid

   #99280

   Purpose

   Can be used to generate AAV virus that will express mScarlet in the presence of Cre

   Depositor

   Rylan Larsen

   Insert

   mScarlet
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG PSAM4-5HT3 HC IRES EGFP

   Plasmid

   #119740

   Purpose

   Chemogenetic activator expression plasmid

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2019 Mar 14. pii: science.aav52

   Insert

   PSAM4 a75HT3 HC (homo) IRES eGFP (CHRNA7, HTR3A Human, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L131G, Q139L, Y217F, R420Q, R424D, R428A

   Promoter

   CAG

   Available since

   March 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG PSAM4 GlyR IRES EGFP

   Plasmid

   #119739

   Purpose

   Chemogenetic inhibitor expression plasmid

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2019 Mar 14. pii: science.aav52

   Insert

   PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L131G, Q139L, Y217F

   Promoter

   CAG

   Available since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV1 for Split-PE, SpCas9 nickase (PKC1001)

   Plasmid

   #190111

   Purpose

   AAV1 for expression of nickase SpCas9(H840A) for Split-PE use

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

   Insert

   nSpCas9
   

   Use

   AAV

   Tags

   bpNLS

   Expression

   Mutation

   H840A

   Promoter

   EF1a

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG PSAM4 5HT3 LC IRES GFP

   Plasmid

   #123066

   Purpose

   Chemogenetic activator expression plasmid

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2019 Mar 14. pii: science.aav52

   Insert

   PSAM4 5HT3 LC IRES GFP (Htr3a Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L131G Q139L Y217F

   Promoter

   CAG

   Available since

   April 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-pCALM1-EYFP-WPRE-bGHpA

   Plasmid

   #213971

   Purpose

   AAV expression of EYFP from pCALM1 promoter

   Depositor

   Zhonghua Lu

   Article

   Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

   Insert

   pCALM1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-SpCas9(H840A)-T2A-EGFP

    Plasmid

    #221231

    Purpose

    Express nCas9 nickase (H840A) with GFP reporter

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    SpCas9(H840A)-T2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H840A

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. CROPseq-EFS-HypaSpCas9-P2A-puro

    Plasmid

    #106965

    Purpose

    single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen

    Depositor

    Alex Hewitt

    Insert

    HypaSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcs2+spCas9 ABE-Umax-nanos

    Plasmid

    #222142

    Purpose

    Adenine base editor with modified TadA variant for germline A-to-G base editing in zebrafish

    Depositor

    Gaurav Varshney

    Article

    Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

    Insert

    Modified TadA-nSpCas9 with nanos UTR
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Bipartite NLS

    Expression

    Mutation

    Promoter

    SP6

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

    Plasmid

    #139987

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV pCAG-FLEX-EGFP-WPRE

    Plasmid

    #51502

    Purpose

    Can be used to generate AAV virus that will express EGFP in the presence of Cre

    Depositor

    Hongkui Zeng

    Article

    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. R26-pCAG-Ceru-10unit-BGHpA

    Plasmid

    #158387

    Purpose

    10-unit intMEMOIR recording array for mammalian cells

    Depositor

    Michael Elowitz

    Article

    Chow et al Science. 2021 Apr 9;372(6538). pii: 372/

    Insert

    intMEMOIR 10-unit array
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAMBIA-FLS2prom::FLS2-3xmyc-GFP

    Plasmid

    #86157

    Purpose

    FLS2-GFP expresssion

    Depositor

    Silke Robatzek

    Article

    Robatzek et al Genes Dev. 2006 Mar 1;20(5):537-42.

    Insert

    FLS2 (FLS2 Mustard Weed)
    

    Use

    Tags

    3xmyc and GFP

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSpCas9nucl-T2A-mCherry - BAKgRNA1- BAKgRNA2

    Plasmid

    #167295

    Purpose

    Plasmid encoding for 2 gRNAs targeting the human BAK gene and a CMV driven nuclease Cas9 followed by self-cleaving mCherry

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    BAK (BAK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-CBE4max-SpRYc-P2A-EGFP

    Plasmid

    #208336

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRYc

    Depositor

    Pranam Chatterjee

    Article

    Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:

    Insert

    human codon optimized CBE4max Cas9 variant named SpRYc with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    Sc++ (1-1119), SpRY (1111-1368), D10A

    Promoter

    CAG

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSpCas9nucl-T2A-EGFP - BAXgRNA1- BAXgRNA2

    Plasmid

    #167296

    Purpose

    Plasmid encoding for 2 gRNAs targeting the human BAX gene and a CMV driven nuclease Cas9 followed by self-cleaving EGFP

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    BAX (BAX Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCA14-pHR-SFFV-GFP-RPA43

    Plasmid

    #199445

    Purpose

    Expression of GFP-RPA43 in mammalian cells

    Depositor

    Baohui Chen

    Article

    Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

    Insert

    RPA43 (POLR1F Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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