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pEN_TGmiRc3

Plasmid

#25749

Purpose

Entry vector for cloning miR30-based shRNA driven by TRE promoter with GFP coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Tags

GFP

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::CreERT2-bGHpA

Plasmid

#62444

Purpose

MXS_chaining vector with PGK::CreERT2-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

cassette containing the tamoxifen inducible Cre recombinase with PGK Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_CMV::CreERT2-bGHpA

Plasmid

#62443

Purpose

MXS_chaining vector with CMV::CreERT2-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

cassette containing the tamoxifen inducible Cre recombinase with CMV Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 Orf9c

Plasmid

#158379

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 Orf9c

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 28, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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EmptyTranscriptionalReporter

Plasmid

#62453

Purpose

MXS_chaining vector with 2 cassettes

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Inserts

CMVmin::NLS-Citrine-bGHpA
CMV::NLS-Cherry-bGHpA

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0068

Plasmid

#177025

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

major latex protein-like

Use

Synthetic Biology

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pGAGmEos2-N1

Plasmid

#46372

Depositor

Suliana Manley

Article

Gunzenhauser et al Nano Lett. 2012 Sep 12;12(9):4705-10. do

Insert

Gag

Tags

mEos2

Expression

Mammalian

Mutation

Rev independentHXB2 Gag sequence

Promoter

CMV

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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pETM41-EcPPXc

Plasmid

#38329

Depositor

Florian Freimoser

Article

Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

Insert

E. coli polyphosphatase (ppx Escherichia coli)

Tags

His, MBP, and TEV

Expression

Bacterial

Mutation

C-terminus of E. coli polyphosphatase (described …

Promoter

T7

Available Since

Aug. 6, 2012

Availability

Academic Institutions and Nonprofits only
pET21a - APX

Plasmid

#89618

Purpose

Expresses recombinant 6HIS tagged T. cruzi ascorbate-dependent tryparedoxin peroxidase protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Ascorbate dependent Tryparedoxin peroxidase

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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pME-AKT-PH

Plasmid

#67674

Purpose

Gateway entry clone containing AKT-PH with no stop codon

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

AKT-PH

Use

Gateway multisite middle entry clone

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pKM263-EcPPXc

Plasmid

#38328

Depositor

Florian Freimoser

Article

Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

Insert

E. coli polyphosphatase (ppx Escherichia coli)

Tags

6xHIS, GST, and TEV

Expression

Bacterial

Mutation

C-terminus of E. coli polyphosphatase (described …

Promoter

T7

Available Since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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BcLOV-mCh-SOS

Plasmid

#208279

Purpose

Expresses BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Article

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

BcLOV-mCh-SOS

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pFBCPDL6

Plasmid

#67285

Purpose

CPD photolyase from Drosophila with Flag tag in pFastbac vector. Intermediate in insect cell expression

Depositor

Aziz Sancar

Article

Selby et al Biochemistry. 2012 Jan 10;51(1):167-71.

Insert

CPD photolyase (phr Fly)

Use

Bacterial vector, insert not expressed

Tags

Flag

Mutation

V275E*

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF4A02

Plasmid

#71021

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Ube3a (Ube3a Fly)

Use

Gateway entry vector

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
MAC-SARS-CoV-2 Orf3b

Plasmid

#158373

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 Orf3b

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 26, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR221-Firefly

Plasmid

#79495

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Firefly Luciferase CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

Firefly Luciferase

Use

Multisite gateway entry clone

Promoter

no Promoter

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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MXS_CMV::NeoR-bGHpA

Plasmid

#62437

Purpose

MXS_chaining vector with CMV::NeoR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against Neomycin with CMV Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::PuroR-bGHpA

Plasmid

#62440

Purpose

MXS_chaining vector with PGK::PuroR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against Puromycin with PGK Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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IO001: pMVP (R4-R3) eGFP

Plasmid

#121730

Purpose

pMVP R4-R3 entry plasmid, contains eGFP for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eGFP

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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p3E-ML1N*2(7Q)

Plasmid

#67679

Purpose

Multisite gateway entry clone for adding C-terminal fusions of p3E-ML1N*2(7Q)

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

ML1N*2(7Q)

Use

Gateway multisite 3' entry clone

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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