We narrowed to 7,331 results for: 11

1. pET28a-R4-uAb

   Plasmid

   #101801

   Purpose

   Bacterial cell expression of the beta-galactosidase-specific ubiquibody, R4-uAb

   Depositor

   Matthew DeLisa

   Article

   Portnoff et al J Biol Chem. 2014 Mar 14;289(11):7844-55

   Insert

   R4-uAb
   

   Tags

   6xHis and Flag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMLS791

   Plasmid

   #188889

   Purpose

   Targeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker, Phsp16.41::Cre and Pmyo-2::2xNLS::cyOFP

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

   Insert

   floxed Cbr-unc-119 marker, Phsp16.41::Cre and Pmyo-2::2xNLS::cyOFP
   

   Expression

   Worm

   Mutation

   C. elegans codom optimization and intron addition

   Available Since

   Aug. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-nDsRedFL-miR124

   Plasmid

   #177806

   Purpose

   The pre-miR-124 sequence has been inserted into the EcoRV of pCAG-nDsRedFL-intron.

   Depositor

   Rikako Sanuki

   Article

   Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

   Insert

   miR-124
   

   Expression

   Mammalian

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIntegrase_10

   Plasmid

   #60581

   Purpose

   plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Integrase_10
   

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pBAD

   Available Since

   March 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. hisSUMO-ZFP57

   Plasmid

   #102716

   Purpose

   Express DNA binding domain of human Zinc finger protein 57 in E. coli

   Depositor

   Gary Stormo

   Article

   Zuo et al Sci Adv. 2017 Nov 17;3(11):eaao1799. doi

   Insert

   Zinc finger protein 57 (ZNF57 Human)
   

   Tags

   hisSUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIntegrase_11

   Plasmid

   #60582

   Purpose

   plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Integrase_11
   

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pBAD

   Available Since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTP443

   Plasmid

   #216361

   Purpose

   xNup98-Nb1t (+1Cys) Nanobody production

   Depositor

   Dirk Görlich

   Article

   Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

   Insert

   xNup98-Nb1t (+1Cys)
   

   Use

   Affinity Reagent/ Antibody

   Tags

   14xHis-NEDD8

   Expression

   Bacterial

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET22b-T5-FB-E25TAG

   Plasmid

   #176545

   Purpose

   Expresses IgG affinity protein FB with a TAG stop codon at E25 position

   Depositor

   Han Xiao

   Article

   Yu et al Bioconjug Chem. 2018 Nov 21;29(11):3522-

   Insert

   IgG affinity protein
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   change glutamic acid 25 to amber stop codon

   Promoter

   T5

   Available Since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pT7-agrBD-I

   Plasmid

   #53439

   Purpose

   T7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220

   Depositor

   Cynthia Collins

   Article

   Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

   Insert

   agrBD locus
   

   Use

   Synthetic Biology

   Tags

   V5 epitope, 6x HIS (not expressed on agrB or agrD…

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7-lac

   Available Since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pReporter_2

    Plasmid

    #60563

    Purpose

    A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Reporter_2
    

    Expression

    Bacterial

    Promoter

    BBa_J23119

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEGFP-C1-hRabin8 F201A

    Plasmid

    #26728

    Depositor

    Keith Mostov

    Article

    Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

    Insert

    Rabin8 (RAB3IP Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    F201A: GEF domain mutant

    Available Since

    Dec. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pEGFP-C1-hRabin8 L196A

    Plasmid

    #26727

    Depositor

    Keith Mostov

    Article

    Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

    Insert

    Rabin8 (RAB3IP Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    L196A: GEF domain mutant.

    Available Since

    Dec. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

13. 645 pCS2-etv2

    Plasmid

    #49005

    Purpose

    Mammalian Expression vector, In-situ probe template and in vitro transcription vector for zebrafish Etv2

    Depositor

    Nathan Lawson

    Article

    Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

    Insert

    ets variant gene 2 (etsrp Zebrafish)
    

    Use

    Invitro transcription, riboprobe template

    Expression

    Mammalian

    Available Since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA-NLS-ZapCmR2

    Plasmid

    #59012

    Purpose

    Nuclear zinc-binding FRET biosensor

    Depositor

    Amy Palmer

    Article

    Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

    Insert

    NLS-ZapCmR2 genetically encoded Zn(II) sensor
    

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    See comment

    Promoter

    CMV

    Available Since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pAG25-L-IFP-F[1] (clon 3)

    Plasmid

    #52899

    Purpose

    Plasmid template for PCR of cassettes for HR, used to introduce IFP F[1] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.

    Depositor

    Stephen Michnick

    Article

    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

    Insert

    L-IFP-F[1]
    

    Use

    Vector for homologous recombination

    Promoter

    TEF promoter

    Available Since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pAG32-L-IFP-F[2] (clon 3)

    Plasmid

    #52900

    Purpose

    Plasmid template for PCR of cassettes for HR, used to introduce IFP F[2] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.

    Depositor

    Stephen Michnick

    Article

    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

    Insert

    L-IFP-F[2]
    

    Use

    Vector for homologous recombination

    Promoter

    TEF promoter

    Available Since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pNLRP11major.MYC

    Plasmid

    #131196

    Purpose

    Mammalian expression of human NLRP11 188S

    Depositor

    Thomas Kufer

    Article

    Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

    Insert

    NLRP11 188S (NLRP11 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    188S

    Promoter

    pCMV

    Available Since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3 Tat K41A HA (P#37)

    Plasmid

    #14655

    Depositor

    Matija Peterlin

    Article

    Cujec et al Genes Dev. 1997 Oct 15. 11(20):2645-57.

    Insert

    Tat (tat HIV)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K41A

    Available Since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

19. FabF (KASII) pet28 N-terminal His Tag

    Plasmid

    #75017

    Purpose

    Expresses the ketosynthase FabF/KASII from the E. coli fatty acid synthase

    Depositor

    Michael Burkart

    Article

    Worthington et al ACS Chem Biol. 2006 Dec 20;1(11):687-91.

    Insert

    FabF (fabF E. coli)
    

    Tags

    His Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 21, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pEGFP-C1-hEFA6B

    Plasmid

    #206413

    Purpose

    Mammalian expression of hEFA6B

    Depositor

    Kazuhisa Nakayama, Hye-Won Shin

    Article

    Ueda et al FEBS Lett. 2013 Jun 5;587(11):1617-23. d

    Insert

    EFA6B (PSD4 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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