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pET21-pelB-LaG94-1

Plasmid

#228961

Purpose

For bacterial expression of anti-GFP nanobody LaG94-1, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-1

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaMIgG-9

Plasmid

#228993

Purpose

For bacterial expression of anti-mouse IgG nanobody LaMIgG-9, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaMIgG-9

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaTdT-44

Plasmid

#228981

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-44, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-44

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaTdT-45

Plasmid

#228982

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-45, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-45

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaMIgG-8

Plasmid

#228992

Purpose

For bacterial expression of anti-mouse IgG nanobody LaMIgG-8, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaMIgG-8

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaTdT-10

Plasmid

#228978

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-10, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-10

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaG94-4

Plasmid

#228964

Purpose

For bacterial expression of anti-GFP nanobody LaG94-4, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-4

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-GST-5

Plasmid

#228987

Purpose

For bacterial expression of anti-GST nanobody GST-5, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

GST-5

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-GST-6

Plasmid

#228988

Purpose

For bacterial expression of anti-GST nanobody GST-6, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

GST-6

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaTdT-8

Plasmid

#228976

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-8, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-8

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaTdT-9

Plasmid

#228977

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-9, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-9

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGG-PIP61

Plasmid

#196799

Purpose

Contains HRM1 promoter

Depositor

Marc Somssich

Publication

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

HYPERSENSITIVE RESPONSE MARKER 1 (AT5G17760 Mustard Weed)

Use

Greengate (gg) entry vector

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP67

Plasmid

#196805

Purpose

Contains SULTR4;1 promoter

Depositor

Marc Somssich

Publication

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

SULFATE TRANSPORTER 4.1 (SULTR4;1 Mustard Weed)

Use

Greengate (gg) entry vector

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP34

Plasmid

#196772

Purpose

Contains HPCA1 promoter

Depositor

Marc Somssich

Publication

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

HP-INDUCED Ca2+ INCREASES 1 (AT5G49760 Mustard Weed)

Use

Greengate (gg) entry vector

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP43

Plasmid

#196781

Purpose

Contains WRKY53 promoter

Depositor

Marc Somssich

Publication

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

WRKY DNA-BINDING PROTEIN 53 (WRKY53 Mustard Weed)

Use

Greengate (gg) entry vector

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP05

Plasmid

#196743

Purpose

Contains SCOOP12 promoter

Depositor

Marc Somssich

Publication

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

PRECURSOR OF SERINE-RICH ENDOGENOUS PEPTIDE 12 (AT5G44585 Mustard Weed)

Use

Greengate (gg) entry vector

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP08

Plasmid

#196746

Purpose

Contains CIPP1 promoter

Depositor

Marc Somssich

Publication

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

CERK1-INTERACTING PROTEIN PHOSPHATASE 1 (AT1G34750 Mustard Weed)

Use

Greengate (gg) entry vector

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pACB113

Plasmid

#222212

Purpose

For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the W15C mutation and a C-terminal His tag

Depositor

Gregg Beckham

Publication

Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

Insert

fghA(W15C)

Tags

6x His

Expression

Bacterial

Mutation

Changed Tryptophan 15 to Cysteine

Promoter

T7

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-HsNRF2 (NFE2L2)

Plasmid

#194302

Purpose

Multisite gateway entry clone for expression of human NRF2 (NFE2L2) with fusion tag at the N-terminus. Parton lab clone KRS

Depositor

Rob Parton

Publication

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

NFE2L2 (NFE2L2 Human)

Use

Multisite gateway entry vector

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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p3E-DrCavin1b

Plasmid

#194292

Purpose

Multisite gateway entry clone for expression of codon optimised zebrafish cavin1b with fusion tag at the N-terminus. Parton lab clone KXJ

Depositor

Rob Parton

Publication

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

cavin1b (cavin1b Zebrafish)

Use

Multisite gateway entry vector

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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