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pTRE3G-marmoset cGASdeltaN-HA

Plasmid

#186863

Purpose

Doxycycline-dependent expression of marmoset cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Publication

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Marmoset cGAS truncation mutant (160-547 a.a.) with C-terminal HA tag

Use

Retroviral

Tags

HA

Mutation

N-terminal truncation

Promoter

TRE3G

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTRE3G-marmoset cGAS-HA

Plasmid

#186864

Purpose

Doxycycline-dependent expression of marmoset cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Publication

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Marmoset cGAS with C-terminal HA tag

Use

Retroviral

Tags

HA

Promoter

TRE3G

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pETSUMOSyt1C2AB(80 JuxtaK-421)

Plasmid

#209008

Purpose

Recombinant protein expression of truncated syt1 construct with Juxta K mutations

Depositor

Edwin Chapman

Publication

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Tags

6xHis-SUMO

Expression

Bacterial

Mutation

aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pETSUMOSyt1C2AB(143-421)

Plasmid

#209007

Purpose

Recombinant protein expression of truncated syt1 construct

Depositor

Edwin Chapman

Publication

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Tags

6xHis-SUMO

Expression

Bacterial

Mutation

aa 143-421 only

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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MOSMO_pcDNA6.2/EmGFP-Bsd

Plasmid

#176975

Purpose

Mammalian expression vector encoding MOSMO and EmGFP-Bsd

Depositor

Roger Reeves

Publication

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

MOSMO (C16orf52 Human)

Expression

Mammalian

Promoter

CMV

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmOskar_C

Plasmid

#146008

Purpose

Insect Expression of DmOskar

Depositor

Elisa Izaurralde

Insert

DmOskar (osk Fly)

Expression

Insect

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM321 - array co-injection fragment mosTI hygroR

Plasmid

#159828

Purpose

Single copy or array insertion by MosTI using split hygroR selection

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

array co-injection fragment mosTI hygroR

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMOS014E: Entry vector for: Grx1-roGFP2 glutathione redox sensor (cytosolic)

Plasmid

#163072

Purpose

Entry vector for: Grx1-roGFP2 glutathione redox sensor (cytosolic)

Depositor

Adam Cohen

Publication

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

Grx1-roGFP2

Use

Gateway Cloning

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMOS016E: Entry vector for: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)

Plasmid

#163075

Purpose

Entry vector for: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)

Depositor

Adam Cohen

Publication

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

roGFP2-Orp1

Use

Gateway Cloning

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMOS028E: Entry vector for: Mitycam calcium sensor (mitochondrial)

Plasmid

#163065

Purpose

Entry vector for: Mitycam calcium sensor (mitochondrial)

Depositor

Adam Cohen

Publication

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

Mitycam

Use

Gateway Cloning

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pSEM271 - MCS mosTI NeoR target

Plasmid

#159830

Purpose

Single copy or array insertion by MosTI using split NeoR selection

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

MCS mosTI NeoR target

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-GST-CHD5(Chromos)-FRAG

Plasmid

#68875

Purpose

IPTG inducible bacterial expression of CHD5 fragment

Depositor

Adrian Bracken

Publication

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5 (CHD5 Human)

Tags

GST

Expression

Bacterial

Mutation

only the two chromodomains of CHD5

Promoter

tac

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only
pSEM388 - mosTI unc-119 - NNN - mScarlet - tbb-2

Plasmid

#182348

Purpose

mScarlet fusion cloning vector for mosTI(unc-119). Transgene inserted upstream of mScarlet and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM390 - mosTI unc-119 - Peft-3 - NNN - tbb-2

Plasmid

#182350

Purpose

Ubiquitous expression cloning vector for mosTI(unc-119). Transgene inserted between Peft-3 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM391 - mosTI unc-119 - Pmex-5 - NNN - tbb-2

Plasmid

#182351

Purpose

Germline expression cloning vector for mosTI(unc-119). Transgene inserted between Pmex-5 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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Triple ORF FRT ifgMosaic Donor (IG135)

Plasmid

#99626

Purpose

To clone the 3 ORFs for FlpO dependent generation of ifgMosaics

Depositor

Rui Benedito

Publication

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

PGK-Neo

Use

Cre/Lox, Mouse Targeting, and Synthetic Biology

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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4xnr UAS ifgMosaic Donor vector (IG230)

Plasmid

#99631

Purpose

To clone the 3 ORFs for Gal4 dependent generation of ifgMosaics

Depositor

Rui Benedito

Publication

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

N-PhiM

Use

Cre/Lox

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSKI074

Plasmid

#11571

Depositor

Detlef Weigel

Publication

Weigel et al Plant Physiol. 2000 Apr . 122(4):1003-13

Insert

CaMV 35S enhancers

Use

Activation tagging

Expression

Plant

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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TvMT Thermosynechococcus vulcanus Metallothionein

Plasmid

#200299

Purpose

Bacterial expression plasmids for production of recombinant Thermosynechococcus vulcanus Metallothionein

Depositor

Artur Krężel

Publication

Mosna et al Metallomics. 2023 Oct 4;15(10):mfad061.

Insert

Gene of metallothionein with Chiting Binding Domanin-Intein

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Available since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-DmOskar-PC_Q

Plasmid

#147323

Purpose

Bacterial Expression of DmOskar-PC

Depositor

Elisa Izaurralde

Insert

DmOskar-PC (osk Fly)

Expression

Bacterial

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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