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Showing: 1 - 20 of 576 results
  1. Mammalian RNAi Tools

    Type
    Collection
    ...expression and knockdown. Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P. Nat Methods. 2006. Feb;3(2...
  2. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Updated
    Oct. 13, 2019, 9:18 p.m.
    .... What aspect of GeoCas9 are you most excited about? Thermostability? Longevity in human plasma? Something...cocaine esterase engineered to have increased thermostability also had a longer lifetime in human blood. ...lower than optimal temperatures. GeoCas9’s thermostability could also be used with in vitro molecular ...CRISPRing! References Harrington, Lucas, et al. "A thermostable Cas9 with increased lifetime in human plasma... thermostable cas9...
  3. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Updated
    Jan. 3, 2020, 7:47 p.m.
    ...post. There’s so many great tips for getting the most from your conference experience so we unfortunately...them all. What are your top tips for getting the most out of your scientific conference? Resources on...
  4. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Updated
    Nov. 18, 2019, 12:30 a.m.
    ...reproducibility. Read on for some tips for getting the most out of your lentiviral transduction experiments....point to consider is how the virus is being titered. Most titering approaches transduce into highly permissive...
  5. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    Updated
    Dec. 31, 2019, 2:26 p.m.
    ... other Addgene news Find the most recent plasmid blog posts Find the most recent CRISPR blog posts Resources...
  6. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

    Type
    Blog Post
    Updated
    Oct. 14, 2019, 1:26 a.m.
    .... Our most popular kit of the year was the Golden Gate TALEN Kit, which continues to be our most requested...distributed through Addgene over our first 10 years. 10 most distributed plasmid technologies pSV-PGC1 - Ok,...Fire's set of vectors for C. elegans research was our most requested item of 2005. shRNA - In 2006, we saw ...two plasmids from Didier Trono's lab are our two most popular plasmids of the past 10 years. Induced Pluripotent... Yamanaka's lab and James Thomson's lab were the most frequently requested. Ubiquitin - One particular...interest in from 2008 to 2009 was ubiquitin. The most requested ubiquitin plasmids were pRK5-HA-Ubiquitin-WT...
  7. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type
    Blog Post
    Updated
    Nov. 12, 2019, 2 p.m.
    ... the Quake Lab. Identifying IgnaviCas9, a hyperthermostable Cas9 This year it was wonderful to learn that...Ignavibacteriae phylum genome. IgnaviCas9 is a hyperthermostable Cas9 CRISPR nuclease that is active up to ...optimal sgRNA spacer length of 22-25 nts, and is thermostable between 31-100°C. They published their finding... in a bioRxiv article and in PNAS. Two other thermostable Cas9 enzymes have previously been characterized...ThermoCas9, IgnaviCas9 is a type II-C Cas9. IgnaviCas9 is most similar to a type II-C Cas9 from Parvibaculum lavamentivorans...What can you do with IgnaviCas9? Why would a thermostable Cas9 be useful? It can enable applications where...steps in the protocol. IgnaviCas9’s incredible thermostability makes the enzyme particularly useful. Additional...
  8. Plasmids for Stem Cell Research

    Type
    Collection
    ...2011 Oct 23;13(11):1353-60. doi: 10.1038/ncb2366. MOS OCT4-2A-SOX2 (Homo sapiens) Mammalian Expression ...
  9. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...2016 Jan 18. doi: 10.1038/nbt.3437. MOS gRNA (BRDN0001147046) MOS (Homo sapiens) Mammalian Expression,...2016 Jan 18. doi: 10.1038/nbt.3437. MOS gRNA (BRDN0001146861) MOS (Homo sapiens) Mammalian Expression,...2016 Jan 18. doi: 10.1038/nbt.3437. MOS gRNA (BRDN0001146393) MOS (Homo sapiens) Mammalian Expression,... CRISPR, Synthetic Biology Cate Selection of chromosomal DNA libraries using a multiplex CRISPR system...Adenoviral Ventura In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system. Nature...versatile reporter system for CRISPR-mediated chromosomal rearrangements. Genome Biol. 2015 May 28;16(1...
  10. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    Updated
    Oct. 19, 2019, 1:38 p.m.
    ...created and designed for the Hall of Human Life at the MOS. (If you want a fun refresher on the Central Dogma...scientists, part of the Addgene family Perhaps the most heartfelt portion of the entire night was when Melina...
  11. Luciferase Plasmids

    Type
    Collection
    ...promoter ORF57 Promoter (Other) Glaunsinger 120519 RL-Mos Renilla luciferase reporter Renilla luciferase (Mus...gene in vitro and in vivo to use in biosensors. The most common types of luciferase found in Addgene’s collection...
  12. Worm Expression Resources

    Type
    Collection
    ... elegans) Kimble 34874 pCFJ601 - Peft-3 Mos1 transposase Mos1 transposase expression vector for use in...65840 XW82 MosSCI-QUAS::Δpes10::GFP 5XQUAS-delta-pes-10::GFP (Other) Shen 65841 XW83 MosSCI-Pmig-13::QF...) Jorgensen 19332 pJL43.1 - Pglh-2::MosTase::glh-2utr Pglh-2::Mos1 transposase (Caenorhabditis elegans...Jorgensen 19333 pJL44 - Phsp16.48::MosTase::glh-2utr Phsp-16::Mos1 transposase (Caenorhabditis elegans...elegans) Jorgensen 34874 pCFJ601 - Peft-3 Mos1 transposase Mos1 transpoase (Caenorhabditis elegans) Jorgensen...Minimal Mos1, NeoR, [4-3] Gateway (Drosophila melanogaster) Jorgensen 44474 pCFJ908 Minimal Mos1, PuroR...Minimal Mos1, peel-1, [4-3] Gateway (Drosophila melanogaster) Jorgensen 44480 pCFJ909 Minimal Mos1, unc-...
  13. Parkinson's Disease Plasmid Resource

    Type
    Collection
    ...Homo sapiens) Baculovirus pBACgus_SUMOstar 6xHis, HisTEV MJFF pBACgus_Sumostar/LRRK2 1867-2186_G2019S SUMO...Homo sapiens) Baculovirus pBACgus_SUMOstar 6xHis, HisTEV MJFF pBACgus_Sumostar/LRRK2 1867-2186_C2024S/C2025S...Homo sapiens) Baculovirus pBACgus_SUMOstar 6xHis, HisTEV MJFF pBACgus_Sumostar/LRRK2 1867-2186_T2031E SUMO...Homo sapiens) Baculovirus pBACgus_SUMOstar 6xHis, HisTEV MJFF pBACgus_Sumostar/LRRK2 1867-2141 SUMO (Synthetic...Homo sapiens) Baculovirus pBACgus_SUMOstar 6xHis, HisTEV MJFF pBACgus_Sumostar/LRRK2 1867-2161 SUMO (Synthetic...Homo sapiens) Baculovirus pBACgus_SUMOstar 6xHis, HisTEV MJFF pBACgus_Sumostar/LRRK2 1867-2176 SUMO (Synthetic...Homo sapiens) Baculovirus pBACgus_SUMOstar 6xHis, HisTEV MJFF pBACgus_Sumostar/LRRK2 1867-2161_G2019S SUMO...
  14. CRISPR Guide

    Type
    Collection
    ... and adaptability, CRISPR has rapidly become the most popular genome engineering approach. Engineered ...ease of generating gRNAs makes CRISPR one of the most scalable genome editing technologies. This advantage...repair (HDR) pathway The NHEJ repair pathway is the most active repair mechanism, and it frequently causes... information, jump to Plan Your Experiment ). In most cases, NHEJ gives rise to small indels in the target...using conjugation and stably integrated into the chromosome. Browse Plasmids: Activate , Repress/Interfere...and synthesis, as well as the ability to target almost any genomic locus, make CRISPR the ideal genome... viability, and tumor metastasis. Currently, the most popular method for conducting genome-wide screens...
  15. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ...repetitive and non-repetitive genomic loci, as well as chromosome painting in living cells. Visualizing a specific...labeled proteins to the given region. For example, chromosome-specific repetitive loci can be efficiently visualized...co-delivering multiple gRNAs that tile the locus. Chromosome painting requires delivery of hundreds of gRNAs...gRNAs with target sites throughout the chromosome. Mammalian Plasmid Gene/Insert Promoter Selectable Marker...) EFS Pederson Multicolor CRISPR labeling of chromosomal loci in human cells. Proc Natl Acad Sci U S A... SSFV Pederson Multicolor CRISPR labeling of chromosomal loci in human cells. Proc Natl Acad Sci U S A...CMV-TO Pederson Multicolor CRISPR labeling of chromosomal loci in human cells. Proc Natl Acad Sci U S A...
  16. Synthetic Biology - Strains

    Type
    Collection
    ...Kanamycin Isaacs Precise manipulation of bacterial chromosomes by conjugative assembly genome engineering. Nat...Spectinomycin Isaacs Precise manipulation of bacterial chromosomes by conjugative assembly genome engineering. Nat...Tetracycline Isaacs Precise manipulation of bacterial chromosomes by conjugative assembly genome engineering. Nat...HL 713 MG 1655 + lacIq_KanR inserted into the chromosome at the intS site none (Other) Kanamycin Lim Disruption... 28. HL 751 MG 1655 + lacIq inserted into the chromosome at the intS site + Δhfq_KanR none (Other) Kanamycin... 28. HL 852 MG 1655 + lacIq inserted into the chromosome at the intS site + ΔdsrA_KanR none (Other) Kanamycin...28. HL 1120 MG 1655 + lacIq inserted into the chromosome at the intS site + ΔmicC + Δhfq_KanR none (Other...
Showing: 1 - 20 of 576 results