...expression and knockdown. Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P. Nat Methods. 2006. Feb;3(2...

...immune system’s response to foreign bodies, are the most commonly used affinity reagents. Antibodies are ...against the antigen, packaged, and sold. This is the most cost-efficient way to produce antibodies, with the... IgA2 IgD Coexpressed with IgM on the surface of most immature B cells; function is unknown Monomer with...anaphylactic shock Monomer with a valency of 2 IgG The most abundant isotype in blood, also found in tissues...Produced during the primary immune response; found mostly in blood Pentamer with a valency of 10 Antigen ... for exact storage requirements. As a guideline, most antibodies can be stored at 4 °C for short-term ...allow detection of the antibody:protein complex. The most commonly used types of signaling molecules are horseradish...

... and adaptability, CRISPR has rapidly become the most popular genome engineering approach. Engineered ...ease of generating gRNAs makes CRISPR one of the most scalable genome editing technologies. This advantage...repair (HDR) pathway The NHEJ repair pathway is the most active repair mechanism, and it frequently causes... information, jump to Plan Your Experiment ). In most cases, NHEJ gives rise to small indels in the target...using conjugation and stably integrated into the chromosome. Browse Plasmids: Activate , Repress/Interfere...and synthesis, as well as the ability to target almost any genomic locus, make CRISPR the ideal genome... viability, and tumor metastasis. Currently, the most popular method for conducting genome-wide screens...

...CRISPR/Cas9 in Caenorhabditis elegans. Mos1 Based Transgenesis miniMos Transgenesis Kit - Erik Jorgensen Lab... related miniMos plasmids can be found on the Jorgensen Lab page. More information on miniMos and MosSCI...

...dollars spent each year. Unfortunately, the causes of most neurodegenerative diseases are only partly understood...no effective therapies to prevent, treat, or cure most of these disorders. An integrated study of the overlapping...introduction to the genetics associated with some of the most common disorders followed by a list of some of the...ten million people worldwide are living with PD, mostly people over the age of 60. There is presently no...Alzheimer’s Disease Alzheimer’s disease (AD) is the most common cause of dementia. Symptoms include memory...

...repetitive and non-repetitive genomic loci, as well as chromosome painting in living cells. Visualizing a specific...labeled proteins to the given region. For example, chromosome-specific repetitive loci can be efficiently visualized...co-delivering multiple gRNAs that tile the locus. Chromosome painting requires delivery of hundreds of gRNAs...gRNAs with target sites throughout the chromosome. Mammalian Plasmid Gene/Insert Promoter Selectable Marker...

... guides around the region to find the most efficient guide (most guides would work, but they sometimes...multiplexing CRISPR to target multiple genome loci, the most efficient and easiest way is to co-transfect several...homology arms be for efficient recombination? The most we've tried to insert so far has been 1kb. We used...

...addresses", where dCas9 is going to bind on the chromosome. When dCas9 binds to a gene, it blocks the progression...Preprint at bioRxiv Detailed information about chromosomal integration of pLC97 can be found in this article...article: St-Pierre et al., One-Step Cloning and Chromosomal Integration of DNA, ACS Synthetic Biology, 2013...

...localization) the C - t erminal D omain (CTD) The most common missense mutations cluster in the MBD and...R168X, R255X, R270X, R294X, and R306C) account for almost 46% of all Rett syndrome cases. Research Milestones... and the direction and degree of skewing of X-chromosome inactivation. J Med Genet . 44, 148–152. (Link...

...Parkinson's Michael J Fox Foundation MJFF 40386 pBACgus_Sumostar/LRRK2 1867-2186 LRRK2 His, TEV polH Parkinson's...Parkinson's Michael J Fox Foundation MJFF 40387 pBACgus_Sumostar/LRRK2 1867-2186_G2019S LRRK2 His, TEV polH...Parkinson's Michael J Fox Foundation MJFF 40388 pBACgus_Sumostar/LRRK2 1867-2186_C2024S/C2025S LRRK2 His, TEV...Parkinson's Michael J Fox Foundation MJFF 40389 pBACgus_Sumostar/LRRK2 1867-2186_T2031E LRRK2 His, TEV polH...Parkinson's Michael J Fox Foundation MJFF 40390 pBACgus_Sumostar/LRRK2 1867-2141 LRRK2 His, TEV polH Parkinson's...Parkinson's Michael J Fox Foundation MJFF 40391 pBACgus_Sumostar/LRRK2 1867-2161 LRRK2 His, TEV polH Parkinson's...Parkinson's Michael J Fox Foundation MJFF 40392 pBACgus_Sumostar/LRRK2 1867-2176 LRRK2 His, TEV polH Parkinson's...

...presence of specific molecules or other factors. Most often, a conformational change in the sensing region... astrocyte calcium signaling in the hippocampal mossy fiber pathway. Neuron. 2014 Apr 16;82(2):413-29....from ATP-Mg Hydrolysis Contributes to Mitotic Chromosome Condensation. Curr Biol. 2018 Feb 5;28(3):444...intracellular environment of S. cerevisiae . The MOSAIC Kit from the Cohen laboratory includes 19 lentiviral...

...Parasites such as Plasmodium (malaria), Toxoplasma (toxoplasmosis), Trypanosoma (African sleeping sickness), Cryptosporidium...homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs at a much lower...

...by ordering cloning grade DNA for over 200 of our most popular plasmids!...but helps to inform us of which plasmids would be most helpful to requesting scientists. Return to top ...

... provides information for the assembly standards most commonly used by part registries, including the ... Cloning Techniques Guide for an overview of the most common methods. BioBrick BioBrick-2 BglBrick Silver...

...range of dividing and non-dividing cell types with almost 100% efficiency. Unlike lentiviruses or other retroviruses...Adenoviruses can be produced by several methods, the most common of which involves homologous recombination...

...Cancer Impact p53 is the most frequently mutated gene (>50%) in human cancer; most of these mutations are...

...region of DNA in the genome of your favorite species almost as easily as correcting a typo in the latest draft...make arrays from 12-31 repeats. Perhaps one of the most appealing features of the TAL effector arrays is...

...(SP): This is the classical Cas9 protein used in most studies to date. Neisseria meningitidis (NM): Significantly...activator. Streptococcus thermophilus #1 (ST1): Almost as small as NM, ST1 consistently yields slightly...

...small GTPase and constitutively active Ras is the most common oncogene in human cancer....leading to cancer. Constitutively active Ras is the most common oncogene in human cancer, found in around...

...upload. Currently supports: Human, mouse, rat, marmoset, pig, chicken, frog (X. tropicalis and X. laevis...human, rat, rabbit, pig, possum, chicken, dog, mosquito, and stickleback. E-CRISP Identifies gRNA target...