We narrowed to 908 results for: pgem

1. pGEMT-Mef2c-tPT2A-GFP

   Plasmid

   #111771

   Purpose

   Bicistronic construct: Co-express two genes by 2A peptides

   Depositor

   Li Qian

   Article

   Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

   Insert

   Mef2c-tPT2A-GFP (Mef2c Mouse)
   

   Use

   Cloning intermediate

   Tags

   GFP

   Promoter

   T7

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEM-sgMUC1_dual-MTS-3x-Set I

   Plasmid

   #162763

   Purpose

   Separately expressing 3 unique sgMUC1_dual-MTS (SetI)

   Depositor

   Antony K Chen

   Article

   Mao et al Nucleic Acids Res. 2019 Nov 18;47(20):e1

   Insert

   sgMUC1_dual-MTS_1; sgMUC1_dual-MTS_2; sgMUC1_dual-MTS_3
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEMHE-mClover3-MAP4-MTBD

   Plasmid

   #171498

   Purpose

   T7 promotor drives in vitro transcription of mClover3-tagged mouse MAP4-Microtubule Binding Domain mRNA

   Depositor

   Melina Schuh

   Article

   So et al Science. 2019 Jun 28;364(6447). pii: 364

   Insert

   MAP4-MTBD (Map4 Mouse)
   

   Use

   Pgehme

   Tags

   mClover3

   Promoter

   T7

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEM-3XP3.RFP-tan

   Plasmid

   #83474

   Purpose

   RFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.

   Depositor

   Patricia Wittkopp

   Article

   Lamb et al Fly (Austin). 2016 Aug 5:1-12.

   Insert

   3XP3.RFP-tan
   

   Use

   CRISPR

   Expression

   Bacterial and Insect

   Promoter

   3XP3 eye-expression Promoter

   Available Since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEMHE-mCherry-mTrim21ΔRING-Box

   Plasmid

   #105523

   Purpose

   T7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted RING-box mRNA

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   TRIM21 (Trim21 Mouse)
   

   Use

   In vitro transcription

   Tags

   mCherry

   Mutation

   deletion of RING domain (aa124-462)

   Promoter

   T7

   Available Since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. Rab27a cds in pGem-T Easy

   Plasmid

   #80546

   Purpose

   Can be used for in situ hybridisation or other purposes

   Depositor

   Rob Parton

   Article

   Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

   Insert

   rab27a (rab27a Zebrafish)
   

   Available Since

   April 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEM mouse PEA3 [TJ#200]

   Plasmid

   #16346

   Depositor

   Thomas Jessell

   Insert

   PEA3 (Etv4 Mouse)
   

   Expression

   Bacterial

   Available Since

   Feb. 4, 2009

   Availability

   Academic Institutions and Nonprofits only

8. pGEMHE-mEGFP-mTrim21ΔRING-Box

   Plasmid

   #105520

   Purpose

   T7 promotor drives in vitro transcription of mEGFP-tagged mouse TRIM21 with deleted RING-box mRNA

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   TRIM21 (Trim21 Mouse)
   

   Use

   In vitro transcription

   Tags

   mEGFP

   Mutation

   deletion of RING domain (aa124-462)

   Promoter

   T7

   Available Since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEMT-Map7-1333-end

   Plasmid

   #46070

   Depositor

   Edgar Gomes

   Article

   Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

   Insert

   Map7-1333-end (Map7 Mouse)
   

   Expression

   Bacterial

   Available Since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEM-sgMUC4_dual-MTS-3x-Set I

    Plasmid

    #162762

    Purpose

    Separately expressing 3 unique sgMUC4_dual-MTS (SetI)

    Depositor

    Antony K Chen

    Article

    Mao et al Nucleic Acids Res. 2019 Nov 18;47(20):e1

    Insert

    sgMUC4_dual-MTS_1; sgMUC4_dual-MTS_2; sgMUC4_dual-MTS_3
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEM-and1 in situ probe

    Plasmid

    #52102

    Purpose

    actinodin 1 and1 antisense in situ probe linearize w/ ApaI SP6 pol

    Depositor

    Didier Stainier

    Article

    Nolte et al Proteomics. 2015 Feb;15(4):739-51. doi:

    Insert

    actinodin 1 in situ probe (and1 Zebrafish)
    

    Expression

    Bacterial

    Available Since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEM-HE-Venus-bPAC(F198Y)

    Plasmid

    #165487

    Purpose

    Xenopus oocyte expression of soluble photoactivatable adenylyl cyclase derived from bPAC with reduced dark activity

    Depositor

    Thomas Oertner

    Article

    Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

    Insert

    Venus-bPAC(F198Y)-myc
    

    Use

    Cdna expression, xenopus oocyte

    Tags

    Myc and Venus

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEM-T Easy-Gbait-hs-QF2

    Plasmid

    #122563

    Purpose

    Integration of QF2 sequence using the CRISPR/Cas9 system.

    Depositor

    Marnie Halpern

    Article

    Choi et al Elife. 2021 Dec 8;10:e72345. doi: 10.755

    Insert

    Gbait-hsp70-QF2-SV40pA
    

    Use

    Cloning

    Available Since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEMT-halo-HA-p2a-neoR

    Plasmid

    #161977

    Purpose

    homologous donor template for endogenous tagging

    Depositor

    Joanna Wysocka

    Article

    Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

    Insert

    GAlinker-HA-halo-HA-p2a-neoR
    

    Available Since

    Jan. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEMT-puroR-p2a-HA-halo

    Plasmid

    #161979

    Purpose

    homologous donor template for endogenous tagging

    Depositor

    Joanna Wysocka

    Article

    Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

    Insert

    puroR-p2a-HA-halo-GSlinker
    

    Available Since

    Jan. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEM-T Easy-Gbait-hs-Cre

    Plasmid

    #122562

    Purpose

    Integration of Cre sequence using the CRISPR/Cas9 system.

    Depositor

    Marnie Halpern

    Article

    Choi et al Elife. 2021 Dec 8;10:e72345. doi: 10.755

    Insert

    Gbait-hsp70-Cre-SV40pA
    

    Use

    Cloning

    Available Since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEM-sgIGR_dual-MTS-3x-Set I

    Plasmid

    #162764

    Purpose

    Separately expressing 3 unique sgIGR_dual-MTS (SetI)

    Depositor

    Antony K Chen

    Article

    Mao et al Nucleic Acids Res. 2019 Nov 18;47(20):e1

    Insert

    sgIGR_dual-MTS_1; sgIGR_dual-MTS_2; sgIGR_dual-MTS_3
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Rab18b cds in pGem-T Easy

    Plasmid

    #80539

    Purpose

    Can be used for in situ hybridisation or other purposes

    Depositor

    Rob Parton

    Article

    Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

    Insert

    rab18b (rab18b Zebrafish)
    

    Available Since

    April 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. Rab18a cds in pGem-T Easy

    Plasmid

    #80538

    Purpose

    Can be used for in situ hybridisation or other purposes

    Depositor

    Rob Parton

    Article

    Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

    Insert

    rab18a (rab18a Zebrafish)
    

    Available Since

    April 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEMT-Pax7bait-T2a-Cherry-pA

    Plasmid

    #111154

    Purpose

    knock in bait for Axolotl Pax7 locus

    Depositor

    Elly Tanaka

    Article

    Fei et al Proc Natl Acad Sci U S A. 2017 Nov 21;11

    Insert

    Pax7bait-T2a-Cherry-pA
    

    Expression

    Mammalian

    Mutation

    wt

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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