We narrowed to 3,756 results for: biorxiv

1. pLenti-MCS-3AID-HA-2A-mCherryBSD

   Plasmid

   #225700

   Purpose

   Empty backbone for lentiviral expression of AID degron tagged POI and mCherry fused BlasticidinR

   Depositor

   Lacra Bintu

   Article

   Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

   Type

   Empty backbone

   Use

   Lentiviral and Synthetic Biology

   Tags

   T2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   pEF1A

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-rTetR(SE)-VP64-2A-mCherryBSD

   Plasmid

   #225695

   Purpose

   Lentiviral expression of rTetR(SE) fused to VP64 and mCherry fused BlasticidinR

   Depositor

   Lacra Bintu

   Article

   Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

   Insert

   rTetR-VP64
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3xFLAG and T2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAS3-tdc-1P::AI::acc-1::SL2::his-24::tagRFP::unc-54-3'UTR

   Plasmid

   #225926

   Purpose

   Expressing ACC-1 in RIM neuron

   Depositor

   Andrew Leifer

   Article

   Kumar et al bioRxiv [Preprint]. 2024 Mar 27:2024.03.

   Insert

   ACC-1 (acc-1 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   tdc-1

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAS3-tdc-1P::AI::lgc-47::SL2::his-24::tagRFP::unc-54-3'UTR

   Plasmid

   #225925

   Purpose

   Expressing LGC-47 in RIM neuron

   Depositor

   Andrew Leifer

   Article

   Kumar et al bioRxiv [Preprint]. 2024 Mar 27:2024.03.

   Insert

   LGC-47 (lgc-47 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   tdc-1

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNMTMA_MBP-Z-Cav1-His6

   Plasmid

   #223701

   Purpose

   To produce z-spheres (caveospheres containing the anti-IgG Z-domain of protein A), Parton Lab clone LCU

   Depositor

   Rob Parton

   Article

   T. D. et al bioRxiv

   Insert

   MBP-Z-Cav1-His6
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB-cT3G-cERP2-SOD1

   Plasmid

   #222568

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of SOD1

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   SOD1 (SOD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPB-cT3G-cERP2-RB1

   Plasmid

   #222550

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of RB1

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   RB1 (RB1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-cT3G-cERP2-KDM5B

   Plasmid

   #222555

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of KDM5B

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   KDM5B (KDM5B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. LAMTOR1-APEX2

   Plasmid

   #215556

   Purpose

   Stable, inducible expression in lysosome

   Depositor

   Nevan Krogan

   Article

   Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

   Insert

   LAMTOR1-EGFP-Flag tag-APEX2-NES
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. CL7_2xNLS_iGeoCas9(C1)_2xNLS_His

    Plasmid

    #222987

    Purpose

    Bacterial expression of iGeoCas9(C1) construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

    Insert

    CL7_2xNLS_iGeoCas9(C1)_2xNLS_His
    

    Use

    CRISPR

    Tags

    CL7 and His

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223_NKX2-1_HD_160-223AA

    Plasmid

    #221477

    Purpose

    Gateway cloning of NKX2-1 N-terminal and C-terminal truncation (homeodomain only) mutation overexpression

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Deletion of 1-159AA N-terminal and 224-371AA C-te…

    Promoter

    Available since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Efg1-ΔDBD-Y115S/Y132S/Y139S

    Plasmid

    #216408

    Purpose

    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y115/Y132/Y139 to serine mutations

    Depositor

    Nicolas Fawzi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

    Insert

    Efg1-ΔDBD-Y115S/Y132S/Y139S
    

    Use

    Tags

    6xHis-tags

    Expression

    Bacterial

    Mutation

    changed Tyrosine 115, 132 and 139 to Serine, and …

    Promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLX-EF1a_NKX2-1_HD_160-223AA

    Plasmid

    #221489

    Purpose

    NKX2-1 N-terminal and C-terminal truncation (homeodomain only) mutation lentiviral overexpression

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Deletion of 1-159AA N-terminal and 224-371AA C-te…

    Promoter

    EF1a

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE-FKBP-GFP-OPTN

    Plasmid

    #208866

    Purpose

    Stably express GFP-tagged OPTN for CID induced mitophagy

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    OPTN (OPTN Human)
    

    Use

    Tags

    FKBP-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSCL475

    Plasmid

    #219503

    Purpose

    Express Ade2, Can1 and Faa1 retron msd donor-gRNAs from a single Gal7 promoter, separated by a Csy4 recognition sequence, with an msr expressed in trans

    Depositor

    Seth Shipman

    Article

    Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

    Insert

    Ade2, Can1 and Faa1 retron msd donor-gRNAs separated by a Csy4 recognition sequence, with an msr expressed in trans
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL7

    Available since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEF-IRES-puro6 +gal4DBDHIFCAD pGalO linker

    Plasmid

    #207172

    Purpose

    expression plasmid for Gal4DBD-HIF-CAD expression GalO linker

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Insert

    Gal4DBD-HIF1a-CAD GalO linker
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-tet2puro-gal4DBD-HIFCAD

    Plasmid

    #207173

    Purpose

    Dox Inducible Expression Plasmid for gal4DBD-HIF-CAD

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Insert

    Gal4DBD-HIF1a-CAD
    

    Use

    Lentiviral; Dox inducible

    Tags

    2x Myc

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA-MBP-TEVpCS-ADGRF3 CTF-FLAG

    Plasmid

    #217696

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRF3 (ADGRF3 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-752

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. HA-MBP-TEVpCS-ADGRE5 CTF-FLAG

    Plasmid

    #217693

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRE5 (ADGRE5 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-530

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. Efg1-ΔDBD-A51P/A56P/Q443P

    Plasmid

    #216402

    Purpose

    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q443 to proline mutations

    Depositor

    Nicolas Fawzi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

    Insert

    Efg1-ΔDBD-A51P/A56P/Q443P
    

    Use

    Tags

    6xHis-tags

    Expression

    Bacterial

    Mutation

    changed Alanine 51, 56 and Glutamine 443 to Proli…

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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