Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 22,520 results for: tes

Showing: 2581 - 2600 of 22520 results

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HA-MBP-TEVpCS-ADGRG6 CTFdeltaTA-FLAG

Plasmid

#217737

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGR6 (ADGRG6 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-846

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRL2 CTFdeltaTA-FLAG

Plasmid

#217740

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRL2 (ADGRL2 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-830

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-sgRNA(lib)-puro

Plasmid

#119976

Purpose

Lenti sgRNA cloning backbone with CMV-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Wensheng Wei

Article

Liu et al Nat Biotechnol. 2018 Nov 5. pii: nbt.428

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-B/c

Plasmid

#227962

Purpose

Entry plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

Depositor

Alberto Carbonell

Article

Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

B/c

Promoter

None

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRC1 CTFdeltaTA-FLAG

Plasmid

#217717

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

CELSR1 (CELSR1 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-2453

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRB2 CTFdeltaTA-FLAG

Plasmid

#217715

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRB2 (BAI2 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-917

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRV1 CTFdeltaTA-FLAG

Plasmid

#217743

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRV1 (ADGRV1 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-5896

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRL4 CTFdeltaTA-FLAG

Plasmid

#217742

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRL4 (ADGRL4 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-412

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRB3 CTFdeltaTA-FLAG

Plasmid

#217716

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRB3 (BAI3 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-862

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRG1 CTFdeltaTA-FLAG

Plasmid

#217732

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRG1 (ADGRG1 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-388

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRC2 CTFdeltaTA-FLAG

Plasmid

#217718

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

CELSR2 (CELSR2 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-2362

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRD1 CTFdeltaTA-FLAG

Plasmid

#217720

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRD1 (ADGRD1 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-550

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRG4 CTFdeltaTA-FLAG

Plasmid

#217735

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRG4 (ADGRG4 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-2727

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRG2 CTFdeltaTA-FLAG

Plasmid

#217733

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRG2 (ADGRG2 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-612

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pPLN-hFluc-WT-HA-GFP11-KDEL

Plasmid

#177722

Purpose

Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

hFluc

Tags

GFP11, HA, KDEL, and Prolactin Signal Sequence

Expression

Bacterial and Mammalian

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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NLS-AgDD

Plasmid

#80625

Purpose

create nuclear protein aggregates in mammalian cells

Depositor

Thomas Wandless

Article

Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

Insert

NLS-NES-L106P-sfGFP (FKBP1A Human)

Tags

MPPKKKRKVLALKLAGLDI

Expression

Mammalian

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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p403 – TDH3-MTS-mCherry-GFP1-10-His

Plasmid

#177730

Purpose

mCherry and the 1-10th β-strands of GFP (GFP1-10) fused to a mitochondria signal sequence for mitochondrial targeting in yeast plasmid with HIS marker

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

mCherry-GFP1-10

Tags

Mitochondrial Targeting Sequence

Expression

Bacterial and Yeast

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-NES-SspB-MCS-P2A-iLIDcaax

Plasmid

#173869

Purpose

Mammalian expression of mCherry-NES-SspB-P2A-iLIDcaax (opto-control) containing multiple cloning sites (MCS)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mCherry-NES-SspB-MCS-P2A-iLIDcaax

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pATZ-HA-GFP11

Plasmid

#177720

Purpose

ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to an HA tag and the eleventh β-strand of GFP

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

alpha-1-antitrypsin (SERPINA1 Human)

Tags

GFP11 and HA

Expression

Bacterial and Mammalian

Mutation

E342K (ATZ mutant)

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pcdna3 Flag Jip1b

Plasmid

#52123

Purpose

a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

Depositor

Roger Davis

Article

Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

Insert

JNK interacting protein-1b (JIP-1b) (Mapk8ip1 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

April 28, 2014

Availability

Academic Institutions and Nonprofits only

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