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pSA_0_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP11x7

Plasmid

#199490

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Publication

Miles et al Nat Commun. 2024 Jun 12;15(1):5011. doi:

Insert

Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP11x7

Use

CRISPR

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSA_0_mTagBFP2-T2A-sGFP1-10_synCoTC

Plasmid

#199475

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Publication

Miles et al Nat Commun. 2024 Jun 12;15(1):5011. doi:

Insert

mTagBFP2-T2A-sGFP1-10

Use

CRISPR

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSA_0_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2

Plasmid

#199484

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Publication

Miles et al Nat Commun. 2024 Jun 12;15(1):5011. doi:

Insert

Gal4vp16/4xnrUAS-mKate2

Use

CRISPR

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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kank3_pJet1.2

Plasmid

#121981

Purpose

for generating kank3 riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Publication

Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

Insert

kank3 (kank3 Zebrafish)

Use

Cloning vector

Promoter

T7

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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kank4_pJet1.2

Plasmid

#121982

Purpose

for generating kank4 riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Publication

Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

Insert

kank4 (kank4 Zebrafish)

Use

Cloning vector

Promoter

T7

Available since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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ptdTomato-N1-CMVL-ikk1FL(C179A)-tdTomato

Plasmid

#105644

Purpose

Zebrafish Inhibitor of kappa B kinase alpha with mutation at position 179 from cysteine to alanine

Depositor

Sandra Rieger

Publication

Lisse et al J Cell Sci. 2017 Mar 1;130(5):975-988. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CMVL-ikk1FL(C179A)-tdTomato (chuk Zebrafish)

Tags

tdTomato

Expression

Mammalian

Mutation

CMV:ikk1FL(C178A)-tdTomato

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only
1208 p3e-long etv2 3’UTR

Plasmid

#49010

Purpose

Zebrafish Long length etv2 3' UTR in gateway 3' Entry vector

Depositor

Nathan Lawson

Publication

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

ets variant gene 2 (etsrp Zebrafish)

Use

Gateway 3' entry

Mutation

Longest 3' UTR

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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800 pME-Bozozok

Plasmid

#49008

Purpose

full length coding sequence for zebrafish bozozok gene in gateway middle entry vector

Depositor

Nathan Lawson

Publication

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

dharma (dharma )

Use

Gateway middle entry

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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592 p3e-EST etv2 3’UTR

Plasmid

#49003

Purpose

Zebrafish EST annotated length etv2 3' UTR in gateway 3' Entry vector

Depositor

Nathan Lawson

Publication

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

ets variant gene 2 (etsrp Zebrafish)

Use

Gateway 3' entry

Mutation

only the 3' UTR

Promoter

3' gateway entry vector

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pDestTol2-QUAS:GFP

Plasmid

#184811

Purpose

Used to generate the Tg(QUAS:GFP)c578 transgenic line

Depositor

Marnie Halpern

Publication

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

QUAS:GFP-pA

Use

Zebrafish tol2 transgenesis

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pME DN-E-cadherin

Plasmid

#135209

Purpose

Dominate Negative zebrafish e-cadherin label

Depositor

David Tobin

Publication

Cronan et al Immunity. 2016 Oct 18;45(4):861-876. doi

Insert

CDH1

Use

Synthetic Biology

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pRM14

Plasmid

#163693

Purpose

hsp70 promoter inducible expression of DHB:mNeonGreen-P2A-H2B:mScarlet

Depositor

David Matus

Publication

Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

DHB:mNeonGreen-P2A-H2B:mScarlet (HELB, H2BC11 Human)

Use

Unspecified; Zebrafish expression

Tags

mNeonGreen and mScarlet

Promoter

HSP70

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pRM15

Plasmid

#163694

Purpose

hsp70 promoter inducible expression of DHB:mScarlet-P2A-H2B:miRFP670

Depositor

David Matus

Publication

Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

DHB:mScarlet-P2A-H2B:miRFP670 (HELB, H2BC11 Human)

Use

Zebrafish expression

Tags

mScarlet and miRFP670

Promoter

HSP70

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-ING2

Plasmid

#109502

Purpose

Muscle specific zebrafish expression of mKate2 fused to 3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised. Binds PtdIns5P

Depositor

Rob Parton

Publication

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actc1b-mKate2-ING2

Use

Zebrafish plasmids

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2_HA_dreAdar1

Plasmid

#250540

Purpose

In vitro transcription (SP6 promoter) or mammalian expression (CMV promoter) of the catalytic domain of zebrafish Adar1

Depositor

Daniel Cifuentes

Publication

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

HA_dreAdar1

Use

Synthetic Biology

Available since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-SERCA1

Plasmid

#109498

Purpose

Muscle specific zebrafish expression of mKate2 fused to sarcoplasmic/endoplasmic calcium ATPase 1

Depositor

Rob Parton

Publication

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

SERCA1 (atp2a1 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Mutation

Please see Depositor Comments

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-actn2-mKate2

Plasmid

#109751

Purpose

Muscle specific zebrafish expression of mKate2 fused to alpha actinin 2

Depositor

Rob Parton

Publication

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

alpha actinin 2

Use

Zebrafish plasmids

Tags

mKate2

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-CDC42-DN

Plasmid

#109532

Purpose

Muscle specific zebrafish expression of mKate2 fused to dominant negative CDC42

Depositor

Rob Parton

Publication

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CDC42 (cdc42 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Mutation

DN

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-RhoAa-DN

Plasmid

#109526

Purpose

Muscle specific zebrafish expression of mKate2 fused to dominant negative RhoAa

Depositor

Rob Parton

Publication

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

RhoAa (rhoaa Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Mutation

DN

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-EEA1

Plasmid

#109508

Purpose

Muscle specific zebrafish expression of mKate2 fused to early endosome associated 1

Depositor

Rob Parton

Publication

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

EEA1 (eea1 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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