We narrowed to 15,978 results for: grna

1. gRNA giantin/GOLGB1

   Plasmid

   #222315

   Purpose

   CRISPR/Cas9 close to the ATG of giantin/GOLGB1 gene.

   Depositor

   Franck Perez

   Article

   Fourriere et al J Cell Sci. 2016 Sep 1;129(17):3238-50.

   Insert

   gRNA targeting GOLB1 (GOLGB1 Human)
   

   Use

   CRISPR

   Available Since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-nucGFP11-3xPax7gRNA

   Plasmid

   #224570

   Purpose

   Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   GFP11-H2B
   

   Use

   CRISPR

   Tags

   Histone H2B (nuclear localization)

   Mutation

   Codon optimized

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHelper-CRISPRi-sgRNA

   Plasmid

   #221135

   Purpose

   Helper plasmid for sgRNA cloning for CRISPRi. sgRNA-scaffold with dCas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.

   Depositor

   Craig Roy

   Article

   Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

   Insert

   sgRNA-scaffold with dCas9 handle
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   J23119(SpeI)

   Available Since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTBL3401 BtoApegRNA-sfGFP

   Plasmid

   #226667

   Purpose

   PiggyBac cargo vector encoding B to A pegRNA marked with sfGFP

   Depositor

   Chang Liu

   Article

   Loveless et al bioRxiv 2021.11.05.467507

   Insert

   BtoA pegRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pICH86966::AtU6p::sgRNA:NbVPE1a/b

   Plasmid

   #223218

   Purpose

   Expresses an sgRNA targeting the NbVPE1a/b genes in Nicotiana benthamiana with an Arabidopsis U6 promoter

   Depositor

   Tsepo Tsekoa

   Article

   Singh et al Front Plant Sci. 2022 Aug 18;13:953654.

   Insert

   NbVPE1a/b
   

   Use

   CRISPR and Synthetic Biology

   Tags

   None

   Expression

   Plant

   Promoter

   Arabidopsis U6 promoter

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pICH86966::AtU6p::sgRNA:NbCysP6

   Plasmid

   #223217

   Purpose

   Expresses an sgRNA targeting the NbCysP6 gene in Nicotiana benthamiana with an Arabidopsis U6 promoter

   Depositor

   Tsepo Tsekoa

   Article

   Singh et al Front Plant Sci. 2022 Aug 18;13:953654.

   Insert

   NbCysP6 sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   None

   Expression

   Plant

   Promoter

   Arabidopsis U6 promoter

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. FgH1tUTG-sgRNA.FOXA-ZEB2_CRISPRd

   Plasmid

   #216170

   Purpose

   Express the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experiment

   Depositor

   Makiko Iwafuchi

   Article

   Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

   Insert

   sgRNA- FOXA.bs- ZEB2.locus
   

   Use

   Lentiviral

   Tags

   EGFP

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. FgH1tUTG-sgRNA.PRDM1-ZEB2_CRISPRd

   Plasmid

   #216171

   Purpose

   Express the gRNA targeting the PRDM1-binding site in the human ZEB2 locus for the CRISPRd experiment

   Depositor

   Makiko Iwafuchi

   Article

   Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

   Insert

   sgRNA- PRDM1.bs- ZEB2.locus (PRDM1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. FgH1tUTG-sgRNA.Control-ZEB2_CRISPRd

   Plasmid

   #216172

   Purpose

   Express the gRNA targeting the control site in the human ZEB2 locus for the CRISPRd experiment

   Depositor

   Makiko Iwafuchi

   Article

   Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

   Insert

   sgRNA- Control- ZEB2.locus
   

   Use

   Lentiviral

   Tags

   EGFP

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. LRG-sgRNA.FOXA-ZEB2_CRISPRd_v2

    Plasmid

    #216173

    Purpose

    Express the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experiment

    Depositor

    Makiko Iwafuchi

    Article

    Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

    Insert

    sgRNA- FOXA.bs- ZEB2.locus
    

    Use

    Lentiviral

    Tags

    EGFP

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. LentiCRISPRv2 EGFP sgRNA4

    Plasmid

    #164934

    Purpose

    Expresses EGFP sgRNA4 in mammalian cells

    Depositor

    Ji Luo

    Article

    Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

    Insert

    sgRNA4 targeting Enhanced green fluorescent protein (verified for knockout)
    

    Use

    Lentiviral

    Promoter

    EFS promoter

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDR274-gsc2-sgRNA

    Plasmid

    #184818

    Purpose

    Used to synthesize gRNA targeting exon 2 of the gsc2 gene

    Depositor

    Marnie Halpern

    Article

    Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

    Insert

    gsc2-sgRNA
    

    Use

    CRISPR; Template for sgrna synthesis

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. hPLD3-sgRNA-Cas9_mcherry

    Plasmid

    #199342

    Purpose

    encodes sgRNA for human PLD3 KO, (target Exon 7) plus Cas9-P2A-mCherry

    Depositor

    David Nemazee

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    P2A-RFP

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. hPLD4-sgRNA-Cas9_mcherry

    Plasmid

    #199343

    Purpose

    encodes sgRNA for human PLD4 KO, (target Exon 5) plus Cas9-P2A-mCherry

    Depositor

    David Nemazee

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    P2A-mCherry

    Expression

    Mammalian

    Mutation

    sgRNA: accagtagtatgaagccacg

    Promoter

    human U6

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. SHLD3 N-terminal sgRNA

    Plasmid

    #207094

    Purpose

    pX330 based plasmid for expression of Cas9 and the TTACTGCAGAATGACTACAG sgRNA to target the SHLD3 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    TTACTGCAGAATGACTACAG
    

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. NBS1 C-terminal sgRNA

    Plasmid

    #207092

    Purpose

    pX330 based plasmid for expression of Cas9 and the TAAAAAGGAGAAGATAACTG sgRNA to target the NBS1 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    TAAAAAGGAGAAGATAACTG
    

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. RNF169 C-terminal sgRNA

    Plasmid

    #207099

    Purpose

    pX330 based plasmid for expression of Cas9 and the ACACTTCATTAGGTGCTACT sgRNA to target the RNF169 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    ACACTTCATTAGGTGCTACT
    

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. SHLD1 N-terminal sgRNA

    Plasmid

    #207101

    Purpose

    pX330 based plasmid for expression of Cas9 and the ATGGCAGGACTATGGCAGCC sgRNA to target the SHLD1 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    ATGGCAGGACTATGGCAGCC
    

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. ATM C-terminal sgRNA

    Plasmid

    #207097

    Purpose

    pX330 based plasmid for expression of Cas9 and the TTTCTAAAGGCTGAATGAAA sgRNA to target the ATM locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    TTTCTAAAGGCTGAATGAAA
    

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. 3xMS2 TR knockin sgRNA 1

    Plasmid

    #207607

    Purpose

    sgRNA for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locus

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    cgactcgcccggcagcgcac
    

    Tags

    None

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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