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Showing: 2821 - 2836 of 2836 results
  1. pcDNA6-BKαM3αM4-V5-His

    Plasmid
    #255912
    Purpose
    BKαM dual-repeat expressing plasmid in which αM3 (residues 44–649) is preceded by an N-terminal sequence (MGAAAA) containing a NotI site and linked to αM4 (residues 43–649) via a flexible linker.
    Depositor
    Insert
    KCNMA1 (partial) (KCNMA1 Human)
    Tags
    V5-6xHis
    Expression
    Mammalian
    Mutation
    contains αM3 (residues 44–649) preceded by an N-t…
    Available Since
    June 22, 2026
    Availability
    Academic Institutions and Nonprofits only
  2. pLCKO-CMV-SARS-CoV-2-S-Bel-P5_5-7-IRES-mNeonGreen-NES/PKI-P2A-Blasticidin

    Plasmid
    #237480
    Purpose
    Encodes full-length SARS-CoV-2 Spike protein (Belgium/GHB-03021 isolate), featuring the E484D substitution, which likely emerged during passaging and enhances TMEM106B binding.
    Depositor
    Insert
    SARS-CoV-2 Spike protein (Belgium/GHB-03021 isolate)
    Use
    Lentiviral
    Tags
    IRES-mNeonGreen-NES/PKI and P2A-Blasticidin
    Expression
    Mammalian
    Mutation
    S484D + S813I + deleted amino acids 68-76 + 676-6…
    Promoter
    CMV
    Available Since
    July 8, 2025
    Availability
    Academic Institutions and Nonprofits only
  3. pLV-RnSyt1-sgRNA2-dCas9-KRAB-TagBFP2 (identifier AAAA-0246)

    Plasmid
    #202555
    Purpose
    Synaptotagmin-1 sgRNA2 with TagBFP2
    Depositor
    Insert
    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCAGTACTCGCGTGCCTCGCACCGG) (Syt1 Rat)
    Use
    CRISPR and Lentiviral
    Tags
    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. pLV-RnSyt1-sgRNA3-dCas9-KRAB-TagBFP2 (Identifier AAAA-0247)

    Plasmid
    #202556
    Purpose
    Synaptotagmin-1 sgRNA3 with TagBFP2
    Depositor
    Insert
    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCTCCTCCTGCAGCGGCAGCATCGG) (Syt1 Rat)
    Use
    CRISPR and Lentiviral
    Tags
    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. pLV-RnSyt1-sgRNA1-dCas9-KRAB-TagBFP2 (identifier AAAA-0245)

    Plasmid
    #202554
    Purpose
    Synaptotagmin-1 sgRNA1 with TagBFP2
    Depositor
    Insert
    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ -CACCGCGTGCCTCGCACCGGTCCGCGG) (Syt1 R. norvegius (gRNA))
    Use
    CRISPR and Lentiviral
    Tags
    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. LifeAct-mNeonGreen -IRES- Talin head-SpyTag003

    Plasmid
    #210024
    Purpose
    Expression of LifeAct-mNeonGreen and Talin head(1-433)-SpyTag003 in mammalian cells
    Depositor
    Insert
    LifeAct-mNeonGreen co-expressed with Talin head(1-433)-SpyTag003
    Expression
    Mammalian
    Mutation
    Encephalomyocarditis virus internal ribosome entr…
    Promoter
    CMV
    Available Since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. pGGA-FT guide RNA1_7

    Plasmid
    #246797
    Purpose
    A GreenGate entry vector containing a guide RNA expression cassette targeting the AtFT gene with 7 different gRNAs
    Depositor
    Insert
    AtU6-1 promoter-FT guide RNA1-AtU6-1 promoter-FT guide RNA3- (FT Mustard Weed, Synthetic)
    Use
    CRISPR; Greengate cloning entry vector
    Expression
    Plant
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
  8. pcDNA3.1-N-Myc_DW164AA + DED/AAA USP7

    Plasmid
    #131256
    Purpose
    Mammalian expression of N-terminally Myc-tagged USP7, with mutations in the TRAF-like domain (DW164AA) and UBL2 (DE758AA_D764A)
    Depositor
    Insert
    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Contains point mutations that convert USP7 aspart…
    Available Since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
  9. pSems-leader-SNAPf-mXFPm-IFNAR1(28-557)

    Plasmid
    #192784
    Purpose
    Expression of SNAPf and mXFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Insert
    IFNAR1 (IFNAR1 Human)
    Tags
    Ig k-chain leader sequence, SNAPf-tag, and mXFPm
    Expression
    Mammalian
    Mutation
    mXFPm: tryptophan 66 to phenylalanine, gluatmic a…
    Promoter
    CMV
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  10. pDONR201-JIP60ml

    Plasmid
    #104959
    Purpose
    Encodes a modified, active, form of Hordeum vulgare c.v. Golden Promise Jasmonate Induced Protein 60 (JIP60)
    Depositor
    Insert
    Jasmonate Induced Protein 60 (JIP60)
    Use
    Gateway entry vector for lr recombination into ot…
    Mutation
    Replaced amino acids 164 to 187 with a methionine…
    Available Since
    March 1, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 2821 - 2836 of 2836 results