We narrowed to 4,183 results for: biorxiv
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Plasmid#238178PurposeLentiviral CRISPR guide vector expressing a ZNF296 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct CaptureDepositorInsertZNF296 sgRNA CRISPRa (ZNF296 Synthetic, Human)
UseCRISPR and LentiviralTagsGFPExpressionMutationPromoterAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
ptet-lux-Tn7T-Gm
Plasmid#226434PurposeThis vector features a tetracycline-inducible system to regulate luxABCDE reporter expression, optimized for efficiency in P. aeruginosa.DepositorInsertsoptimized TetR
luxABCDE
UseTagsExpressionBacterialMutationPromoterPcon and PtetAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW375 (PB) (ZNF35/IKZF1tar-6bp spacers)x4-H2B-BFP insul CMV-TO-GFP-deimlink-NZF
Plasmid#236177PurposePiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/IKZF1-driven BFP, and GFP-NZF dummy transcription factorDepositorInsertsUseTagsmTagBFP2ExpressionMammalianMutationPromoterCMV and four ZNF35/IKZF1 sitesAvailable sinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HA-GTSE1
Plasmid#224719PurposeFor expression of HA-tagged GTSE1 proteinDepositorInsertGTSE1 (GTSE1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
FUGW U6 gLacZ dCas9-KRAB-T2a-GFP
Plasmid#234883Purposenon-targeting CRISPRi controlDepositorInsertL1HS gRNA
UseCRISPR and LentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
FUGW U6 gL1HSg1dCas9-KRAB-T2a-GFP
Plasmid#234882PurposeL1HS-silencing plasmid (CRISPRi gRNA1)DepositorInsertL1HS-silencing plasmid (CRISPRi gRNA1)
UseCRISPR and LentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD -3D+3N
Plasmid#234614PurposeBacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 3 aspartates mutated to asparagineDepositorInserthnRNPA1_LCD_3DN (HNRNPA1 Human)
UseTags6xHis-TEVExpressionBacterialMutationD214N, D242N, D250NPromoterT7Available sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD -4D+4N
Plasmid#234611PurposeBacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 4 aspartates mutated to asparagineDepositorInserthnRNPA1_LCD_4DN (HNRNPA1 Human)
UseTags6xHis-TEVExpressionBacterialMutationD214N, D242N, D250N, D262NPromoterT7Available sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD -4D+4V
Plasmid#234610PurposeBacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 4 aspartates mutated to valineDepositorInserthnRNPA1_LCD_4DV (HNRNPA1 Human)
UseTags6xHis-TEVExpressionBacterialMutationD214V, D242V, D250V, D262VPromoterT7Available sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD -3D+3V
Plasmid#234613PurposeBacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 3 aspartates mutated to valineDepositorInserthnRNPA1_LCD_3DV (HNRNPA1 Human)
UseTags6xHis-TEVExpressionBacterialMutationD214V, D242V, D250VPromoterT7Available sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW373 (PB) (ZNF35/ZNF250tarv3-6bp spacers)x4-H2B-BFP insul CMV-TO-GFP-deimlink-NZF
Plasmid#236175PurposePiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/ZNF250-driven BFP, and GFP-NZF dummy transcription factorDepositorInsertsUseTagsmTagBFP2ExpressionMammalianMutationPromoterCMV and four ZNF35/ZNF250 sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW488 CMV-TO-NZF-(GGS)x8[G1W S3D S6T G7L S9L G11M G14N G17D G19V G22W]-FRB(T2098L)
Plasmid#236159PurposeExpress NZF transcriptional activation domain fused to a deimmunized linker and mTOR FRB rapamycin binding domain with the T098L mutation, under control of CMV promoter with two TetR binding sitesDepositorInsertNZF-deImmunLink-FRB
UseTagsExpressionMammalianMutationT2098L in FRBPromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW263 CMV-TO-ADAR2dd(E488Q, T501A)-PUF-9R(FLP-IN)
Plasmid#236126PurposePlasmid encoding ADAR2dd attached to PUF-9R under control of CMV promoter with two TetR binding sitesDepositorInsertADAR2dd-PUF-9R (ADARB1 Synthetic, Human)
UseTagsExpressionMammalianMutationE488Q, T501A in ADAR2ddPromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW499 CMV-TO-NZF-(GGS)x8[G1W S3D S6T G7L S9L G11M G14N G17D G19V G22W]-FRB(N2093W, T2098L)
Plasmid#236146PurposePlasmid encoding the NZF transcriptional activation domain attached by a deimmunized linker to FRB with N2093W and T098L mutations, under control of CMV promoter with two TetR binding sitesDepositorInsertNZF-deImmunLink-FRB
UseTagsExpressionMammalianMutationN2093W, T2098L in FRBPromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW374 (PB) (ZNF35/ZNF35tar-6bp spacers)x4-H2B-BFP insul CMV-TO-GFP-deimlink-NZF
Plasmid#236176PurposePiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/ZNF35-driven BFP, and GFP-NZF dummy transcription factorDepositorInsertsUseTagsmTagBFP2ExpressionMammalianMutationPromoterCMV and four ZNF35/ZNF35 sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
KL095 - pTRE3G-Flag-Halo-CTCF_ZF1_mutant-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor
Plasmid#232931PurposeVector to introduce a constitutive rtta3G cassette and a DOX-inducible Flag-Halo-mCTCF ZF1 mutant, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycin selection.DepositorInsertFlag-Halo-CTCF ZF1 deletion mutant
UseMouse TargetingTagsExpressionMammalianMutationDeleted amino acids 264-277PromoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_6xHis-TEV-mCherry-WIPI3
Plasmid#223763PurposeExpression of recombinant protein for purificationDepositorInsertWIPI3 (WDR45B Human)
UseTags6xHis-TEV-mCherryExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FKBP-mEGFP-WIPI3
Plasmid#223768PurposeStable expression and inducible recruitment of protein in mammalian cell cultureDepositorInsertWIPI3 (WDR45B Human)
UseLentiviralTagsFKBP-mEGFPExpressionMutationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_6xHis-TEV-mCherry-WIPI2d
Plasmid#223725PurposeExpression of recombinant protein for purificationDepositorInsertWIPI2d (WIPI2 Human)
UseTags6xHis-TEV-mCherryExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG_WIPI2d-TEV-GST
Plasmid#223799PurposeExpression of recombinant protein for purificationDepositorInsertWIPI2d (WIPI2 Human)
UseTagsTEV-GSTExpressionMammalianMutationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only