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Lifeact-3xmNG::TRP1

Plasmid

#227593

Purpose

Integration vector for expressing Lifeact-3xmNeonGreen

Depositor

Bruce Goode

Publication

Wirshing et al Mol Biol Cell. 2024 Sep 1;35(9):mr7. doi

Insert

Lifeact-3xmNeonGreen::TRP1

Tags

3xmNeonGreen

Expression

Yeast

Mutation

Truncated ABP140 (aa 1-17 only)

Promoter

Abp140

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pGK415-luxTA2-4F

Plasmid

#165971

Purpose

Expresses luxTA(2-4F) mutant

Depositor

Jun Ishii

Publication

Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

Insert

luxR

Use

Synthetic Biology

Tags

Nuclear localization signal and three copies of V…

Expression

Yeast

Mutation

S116Y, W201R, codon-optimized for expression in y…

Promoter

pPGK1 mutant

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pAH233

Plasmid

#121144

Purpose

Expression of gRNA with BbsI placeholder for target cloning

Depositor

Katsunori Tanaka

Publication

Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

Insert

gRNA cassette

Use

CRISPR

Expression

Yeast

Promoter

S.pombe rrk1

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pYes2-TaPCS1

Plasmid

#49767

Purpose

Expresses wheat phytochelatin synthase (TaPCS1) in Saccharomyces Cerevisiae

Depositor

Julian Schroeder

Publication

Clemens et al EMBO J. 1999 Jun 15;18(12):3325-33.

Insert

TaPCS1 (PCS1 Triticum aestivum)

Expression

Yeast

Promoter

GAL1

Available since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pYes2-AtPCS1

Plasmid

#49766

Purpose

Expresses Arabidopsis phytochelatin synthase (AtPCS1) in Saccharomyces Cerevisiae

Depositor

Julian Schroeder

Publication

Clemens et al EMBO J. 1999 Jun 15;18(12):3325-33.

Insert

AtPCS1 (PCS1 Mustard Weed)

Expression

Yeast

Promoter

GAL1

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
pKan

Plasmid

#254147

Purpose

Yeast-E.coli shuttle plasmid_2-micron circle-based

Depositor

Melanie Dobson

Publication

Mereshchuk et al G3 (Bethesda). 2026 Feb 18:jkag012. doi:

Type

Empty backbone

Use

Yeast-e.coli shuttle plasmid

Tags

None

Available since

May 25, 2026

Availability

Academic Institutions and Nonprofits only

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pSB3-Kan

Plasmid

#254146

Purpose

Yeast-E.coli shuttle plasmid_ Z. rouxii pSB3-based

Depositor

Melanie Dobson

Publication

Mereshchuk et al G3 (Bethesda). 2026 Feb 18:jkag012. doi:

Type

Empty backbone

Tags

none

Expression

Yeast

Available since

May 20, 2026

Availability

Academic Institutions and Nonprofits only

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pFa6-TID-FRB-3HA-KanMX6

Plasmid

#228020

Purpose

For TurboID-FRB integration; yeast BioID with KanMX6 marker

Depositor

Joseph Reese

Publication

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FRB

Tags

HA tag

Expression

Yeast

Available since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pFa6-TID-FRB-3HA-HIS3MX6

Plasmid

#228021

Purpose

For TurboID-FRB integration; yeast BioID with HIS3MX6 marker

Depositor

Joseph Reese

Publication

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FRB

Tags

HA tag

Expression

Yeast

Available since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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SJZ2-TRP1

Plasmid

#228256

Purpose

Template plasmid for yeast gene knockout, uses auxotrophic marker TRP1 (Kluyveromyces lactis)

Depositor

Zhiping Xie

Publication

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

TRP1

Expression

Yeast

Promoter

BBC1 S.c.

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pETURALEU

Plasmid

#223207

Purpose

Plasmid for selection/counterslection in budding yeast, containing URA3 and LEU2 markers

Depositor

Sahand Jamal Rahi

Publication

Stojkovic et al G3 (Bethesda). 2024 Dec 17:jkae297. doi:

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pYTK001_E2Aq(2nd)

Plasmid

#190911

Purpose

2A peptide E2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Publication

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

E2A

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK001_O2Aq(2nd)

Plasmid

#190918

Purpose

2A peptide O2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Publication

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

O2A

Use

Synthetic Biology

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK001_P2Aq(1st)

Plasmid

#190912

Purpose

2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use as the first 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Publication

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

P2A

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK001_O2Aq(1st)

Plasmid

#190917

Purpose

2A peptide O2A cloned in yeast MoClo entry vector (pYTK001) for use as the first 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Publication

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

O2A

Use

Synthetic Biology

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK001_O2Ab

Plasmid

#183083

Purpose

2A peptide O2A cloned in yeast MoClo entry vector (pYTK001) for use in bi-cistronic and as the first 2A peptide in tri-cistronic construct assembly

Depositor

Zhen Wang

Publication

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

O2A

Use

Synthetic Biology

Expression

Bacterial

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK001_P2Ab

Plasmid

#183084

Purpose

2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use in bi-cistronic and as the first 2A peptide in tri-cistronic construct assembly

Depositor

Zhen Wang

Publication

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

P2A

Use

Synthetic Biology

Expression

Bacterial

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK001_E2Aq(1st)

Plasmid

#183088

Purpose

2A peptide E2A cloned in yeast MoClo entry vector (pYTK001) for use as the first 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Publication

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

E2A

Use

Synthetic Biology

Expression

Bacterial

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK001_P2Aq(2nd)

Plasmid

#183089

Purpose

2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Publication

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

P2A

Use

Synthetic Biology

Expression

Bacterial

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pRS42K_PCP-VP64_A-PP7

Plasmid

#177156

Purpose

Expresses transactivator (PCP-VP64) and scRNA (A-PP7) in yeast cells

Depositor

Gautam Dantas

Publication

Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

Inserts

PCP-VP64
A-PP7

Tags

SV40 NLS

Expression

Yeast

Promoter

ADH1 promoter and SNR52 promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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