165,652 results

1. pRS314-T7-GFP

   Plasmid

   #33130

   Depositor

   Michael Rosbash

   Article

   Dower et al RNA. 2002 May;8(5):686-97.

   Insert

   GFP with TDH3 5' and 3' UTR (gfp )
   

   Expression

   Yeast

   Promoter

   T7

   Available Since

   Dec. 20, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOpen-NotIR (GC)

   Plasmid

   #165507

   Purpose

   Type II restriction enzyme that recognizes the double-stranded sequence GCGGCCGC and cleaves after C-1.

   Depositor

   FreeGenes Project, Drew Endy, Jennifer Molloy

   Insert

   NotI (GC-rich recognition site)
   

   Use

   Synthetic Biology

   Available Since

   July 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pCDNA3.1-FLAG-SF3B1-WT

   Plasmid

   #82576

   Purpose

   Expresses a codon-optimized ORF of human SF3B1 (wild-type)

   Depositor

   Manoj Pillai

   Article

   Kesarwani et al Oncogene. 2016 Aug 15. doi: 10.1038/onc.

   Insert

   human SF3B1-WT (SF3B1 Human)
   

   Tags

   FLAG peptide

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. hGFAP-GFP

   Plasmid

   #40592

   Depositor

   Albee Messing

   Article

   Zhuo et al Dev Biol. 1997 Jul 1;187(1):36-42.

   Insert

   GFP-S65T
   

   Use

   Mouse Targeting

   Tags

   MP-1 fragment (mouse protamine 1 intron and polya…

   Expression

   Bacterial and Mammalian

   Mutation

   Construct contains a mutated, humanized hGFP(S65T…

   Promoter

   hGFAP promoter

   Available Since

   Oct. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. BB3cK_pGAP_23*_pPFK300_Cas9

   Plasmid

   #104910

   Purpose

   hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418

   Depositor

   Brigitte Gasser

   Article

   Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

   Inserts

   HH - FS23 linker - HDV
   human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   April 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBMNb-cALFA

   Plasmid

   #159991

   Purpose

   For the protein expression with ALFA tag at C-terminus in mammalian cells

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Type

   Empty backbone

   Tags

   HRV3C protease cleavage site-ALFA tag-twin Strep …

   Expression

   Mammalian

   Mutation

   CMV promoter, WPRE

   Available Since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. NES-Grx1-roGFP2

   Plasmid

   #236551

   Purpose

   Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in the cytoplasm.

   Depositor

   Pablo Rivera-Fuentes

   Insert

   NES-Grx1-roGFP2 (NES Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. PZac2.1 gfaABC1D-NLS-SaCas9-3xHA-NLS bGH

   Plasmid

   #178960

   Purpose

   Expresses saCas9 protein specifically in astrocytes

   Depositor

   Baljit Khakh

   Article

   Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

   Insert

   Staphylococcus aureus Cas9 (SaCas9)
   

   Use

   AAV

   Tags

   3x HA

   Promoter

   gfaABC1D (astrocyte specific)

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. miABE

   Plasmid

   #205412

   Purpose

   Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Yingsi Zhou

   Article

   Han et al Nat Methods. 2023 May 25. doi: 10.1038/s

   Insert

   pU6-_RNA*-pCAG-bpNLS-TadA8e(V106W)-32aa-OgeuIscB*(D61A)-GS-bpNLS-GS-TadA8e(V106W)-bpNLS-bGH polyA-pCMV-mCherry-AmpR
   

   Expression

   Mammalian

   Mutation

   D61A+E85R+H369R+S387R+S457R

   Promoter

   hU6, CAG, CMV

   Available Since

   Dec. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUNIV backbone

    Plasmid

    #24705

    Depositor

    Cynthia Czajkowski

    Article

    Venkatachalan et al Pflugers Arch. 2007 Apr . 454(1):155-63.

    Type

    Empty backbone

    Use

    Xenopus oocyte expression

    Expression

    Bacterial, Mammalian, and Y…

    Available Since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEJS2602_AAV-Nme2-ABE8e-i1(V106W)-U6-2xSapI

    Plasmid

    #201684

    Purpose

    All-in-one AAV plasmid expressing Nme2-ABE8e-i1(V106W) and U6 sgRNA cassette. SapI enables cloning new spacers.

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

    Insert

    Nme2-ABE8e-i1 (V106W)
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    U1a

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. GPRC6A-Tango

    Plasmid

    #66386

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPRC6A (GPRC6A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. Pzac2.1 U6-control sgRNA1, 2, 3_gfaABC1D mcherry SV40

    Plasmid

    #179120

    Purpose

    Expresses control sgRNAs under the U6 promoter and mcherry protein specifically in astrocytes

    Depositor

    Baljit Khakh

    Article

    Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

    Insert

    control sgRNA
    

    Use

    AAV

    Promoter

    U6

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti_EF1a_EV

    Plasmid

    #183116

    Purpose

    Empty vector control for pLenti_EF1a constructs

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    Small multiple cloning site
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a_His-TEV-EiCsm6

    Plasmid

    #172487

    Purpose

    Bacterial expression of codon-optimized His-EiCsm6 with a TEV protease cleavage site.

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

    Insert

    Enterococcus italicus Csm6 (codon optimized)
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7 promoter and lac operator

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV_miniENVLPE

    Plasmid

    #232431

    Purpose

    Expression plasmid to produce miniENVLPE particles

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    miniENVLPE
    

    Expression

    Mammalian

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pp-mCAT1

    Plasmid

    #83948

    Purpose

    This plasmid co-expressed with VSVG allows the production of mCAT-1 gesicles rendering target cells permissive to transduction with Ecotropic lenti/retrovectors

    Depositor

    Vincent Lotteau, Philippe Mangeot

    Article

    Mangeot et al Mol Ther. 2011 Sep;19(9):1656-66. doi: 1

    Insert

    mCAT1 (Slc7a1 )
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGRG36-Kn-PA1-GFP

    Plasmid

    #79088

    Purpose

    pGRG36-Kn-PA1-GFP is a Kanamycine resistance plasmid used to integrate a GFP expression cassette into the chromosome of E.coli, described in Yang et al. 2016 (Pending publication Pubmed ID)

    Depositor

    Frank Rosenzweig

    Article

    Yang et al Appl Environ Microbiol. 2020 Apr 1;86(8)

    Insert

    GFP
    

    Use

    Bacterial chromosome integration and expression o…

    Expression

    Bacterial

    Promoter

    PA1

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGAMA-empty

    Plasmid

    #74755

    Purpose

    Mammalian lentiviral vector

    Depositor

    Miguel Ramalho-Santos

    Article

    Qin et al Cell Rep. 2016 Mar 15;14(10):2301-12. do

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    May 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR223-LRPPRC

    Plasmid

    #23521

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    LRPPRC (LRPPRC Human)
    

    Use

    Gateway donor vector

    Mutation

    G246A; deleted E579

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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