165,652 results

1. pC0073 EiCsm6 His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115268

   Purpose

   Expresses EiCsm6 from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   EiCsm6
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   Sept. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-EF1A-CD19.8h.8TM.28.3z

   Plasmid

   #200670

   Purpose

   Modular CD8hinge-CD8transmembrane-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

   Depositor

   Michele Bernasconi

   Article

   Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

   Insert

   FMC63-CD8HD-CD8TM-CD28-CD3z-P2A-EGFP (CD8A, CD28, CD247 Human)
   

   Use

   Lentiviral

   Tags

   MycTag

   Expression

   Mammalian

   Promoter

   EF1a-short

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. EGFP-Cyclin A2

   Plasmid

   #215048

   Purpose

   Expresses EGFP-Cyclin A2 in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CCNA2 (CCNA2 Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-C1-UBF

   Plasmid

   #26672

   Depositor

   Sui Huang

   Article

   Chen et al J Cell Biol. 2001 Apr 2. 153(1):169-76.

   Insert

   UBF1 (UBTF Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Nov. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

5. TFORF3232

   Plasmid

   #144708

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   JUNB (JUNB Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF0756

   Plasmid

   #141685

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   JUND (JUND Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. lenti-NLS-mTagBFP2

   Plasmid

   #216128

   Purpose

   Lentiviral vector for expressing mTagBFP2 localizing to the nucleus

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

   Insert

   EF1a-NuclearLocalizationSignal-mTagBFP2-WPRE
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDRF1-GW ymNeongreen-T2A-mCherry

   Plasmid

   #125700

   Purpose

   Produces equimolar expression of yeast codon optimized mNeongreen and mCherry

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymNeongreen-T2A-mCherry
   

   Expression

   Yeast

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-N1-Flag-humanRBM47

   Plasmid

   #134607

   Purpose

   Plasmid bearing RBM47 coding sequence. Flag tagged is useful for IP and WB assays.

   Depositor

   Silvestro Conticello

   Article

   Chieca et al bioRxiv 2021

   Insert

   Flag-humanRBM47 (RBM47 Human)
   

   Tags

   flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMX/cyclinB1-GFP (27)

    Plasmid

    #26061

    Depositor

    Jonathon Pines

    Article

    Hagting et al Curr Biol. 1999 Jul 1. 9(13):680-9.

    Insert

    Cyclin B1 (CCNB1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Aug. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-mRhubarb720

    Plasmid

    #197212

    Purpose

    Expresses the protein of mRhubarb720 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    mRhubarb720
    

    Use

    AAV

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDrDERusk

    Plasmid

    #213703

    Purpose

    Vector for red-light-induced bacterial gene expression

    Depositor

    Andreas Moeglich

    Article

    Meier et al Nat Commun. 2024 Jun 10;15(1):4876. doi:

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    May 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET3a-hUBA1

    Plasmid

    #63571

    Purpose

    Expression of human UBA1 tagged with His

    Depositor

    Titia Sixma

    Article

    Pichler et al Nat Struct Mol Biol. 2005 Mar;12(3):264-

    Insert

    UBA1 (UBA1 Human)
    

    Expression

    Bacterial

    Mutation

    Isoform 2 (1018 amino acids)

    Promoter

    T7

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGAMA-YAP

    Plasmid

    #74942

    Purpose

    Lentiviral over-expression vector for YAP

    Depositor

    Miguel Ramalho-Santos

    Article

    Qin et al Cell Rep. 2016 Mar 15;14(10):2301-12. do

    Insert

    YAP (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    T2A-mCherry

    Promoter

    EF1α

    Available Since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-ICUE4

    Plasmid

    #181846

    Purpose

    Fourth-generation FRET-based Indicator of cAMP Using Epac. Genetically encoded cAMP indicator. Contains high-sensitivity Epac Q270E mutation.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    ICUE4 (RAPGEF3 Human)
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Glutamine 122 in Epac1 domain mutated to glutamat…

    Promoter

    CMV

    Available Since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEC3109 (pSpy1C-Plac(1)_ffluc)

    Plasmid

    #218514

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Insert

    ffluc
    

    Use

    Luciferase

    Expression

    Bacterial

    Mutation

    deletion of mrfp cassette, mutagenesis of the RBS…

    Promoter

    Plac(1)

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-AKAP-mtagBFP2-UBC6

    Plasmid

    #105007

    Purpose

    Mammalian Expression of mtagBFP2

    Depositor

    Franck Polleux

    Article

    Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

    Insert

    mtagBFP2
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-CMV-GFP-W

    Plasmid

    #196046

    Purpose

    lentiviral expression of the green fluorescent protein driven by cytomegalovirus virus promoter

    Depositor

    Darrell Kotton

    Article

    Wilson et al J Clin Invest. 2010 Jan . 120(1):379-89.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    eGFP

    Promoter

    CMV

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTiGc

    Plasmid

    #78314

    Purpose

    Mycobacterial dual reporter plasmid for fluorescence dilution. Description: hsp60-gfp, hsp60(ribo)-turboFP635 (inducible TurboFP635 under control of theophylline - inducible riboswitch).

    Depositor

    Samantha Sampson

    Article

    Mouton et al Microbiology. 2016 Mar 30. doi: 10.1099/

    Inserts

    TurboFP635
    GFP
    

    Use

    Synthetic Biology; Mycobacterial replicating vect…

    Expression

    Bacterial

    Promoter

    hsp60 and hsp60(ribo)

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pORTMAGE-Ec1

    Plasmid

    #138474

    Purpose

    Enables high-efficiency recombineering in E. coli

    Depositor

    George Church

    Article

    Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

    Inserts

    CspRecT
    mutL E32K
    

    Expression

    Bacterial

    Mutation

    E32K

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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