161,547 results

1. CAG sHRPa-NRX

   Plasmid

   #73143

   Purpose

   Large fragment of split HRP fused to the extracellular terminus of neurexin3B. CAG promoter.

   Depositor

   Alice Ting

   Article

   Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

   Insert

   sHRPa-NRX
   

   Use

   Tags

   2x HA tag and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EF1a-DIO-hM4D(Gi)-mCherry

   Plasmid

   #50461

   Purpose

   Double floxed Gi-coupled hM4D DREADD fused with mCherry under the control of EF1a promoter

   Depositor

   Bryan Roth

   Insert

   hM4D(Gi)-mCherry (CHRM4 Human)
   

   Use

   AAV

   Tags

   HA and mCherry

   Expression

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   EF1a

   Available since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. CAG sHRPb-NLG

   Plasmid

   #73144

   Purpose

   Small fragment of split HRP fused to the extracellular terminus of NLG1. CAG promoter.

   Depositor

   Alice Ting

   Article

   Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

   Insert

   sHRPb-NLG (Nlgn1 Synthetic, Mouse)
   

   Use

   Tags

   V5 tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. SIRT4 Flag

   Plasmid

   #13815

   Purpose

   Mammalian expression of human SIRT4 with flag tag

   Depositor

   Eric Verdin

   Article

   North et al Mol Cell. 2003 Feb . 11(2):437-44.

   Insert

   SIRT4 (SIRT4 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL3-RARE-luciferase

   Plasmid

   #13458

   Depositor

   T. Michael Underhill

   Article

   Hoffman et al J Cell Biol. 2006 Jul 3. 174(1):101-13.

   Insert

   Retinoic Acid Receptor Response Element
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV INS2x mRFP-3MREs (AAV-beta)

   Plasmid

   #206928

   Purpose

   Beta cell-specific gene expression from AAV in human pancreatic islets

   Depositor

   Markus Grompe

   Article

   Chai et al Hum Gene Ther. 2022 Aug;33(15-16):789-80

   Insert

   mRFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   INSx2

   Available since

   Sept. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-HA-BHLHE40

   Plasmid

   #110154

   Purpose

   Expression of human BHLHE40 in mammalian cells

   Depositor

   Daniele Guardavaccaro

   Article

   Kim et al Mol Cell Biol. 2014 Nov 15;34(22):4177-8

   Insert

   BHLHE40 (basic helix-loop-helix family member e40) (BHLHE40 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. (559) pAAV EF-1a KRAB-SadCas9 U6-gRNA

   Plasmid

   #163024

   Purpose

   Expression of CRISPRi with U6-gRNA (Bsa1 sites)

   Depositor

   Tonia Rex

   Article

   Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

   Insert

   Mammalian codon-optimized SadCas9
   

   Use

   AAV

   Tags

   KRAB, SV40 polyA, and myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   hEF-1a

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pST39

   Plasmid

   #64009

   Purpose

   original polycistronic cloning vector for plasmid suite (generation I)

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBa-MARK2-eGFP

    Plasmid

    #66706

    Purpose

    mammalian expression vector for MARK2 with a C-terminal eGFP

    Depositor

    Marvin Bentley, Gary Banker

    Insert

    MARK2 (MARK2 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    chicken beta actin

    Available since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAVS1-Neo-TRE-CMV-Cre-rtTA

    Plasmid

    #165457

    Purpose

    Tetracycline inducible CRE expression from AAVS1 locus

    Depositor

    Madeline Lancaster

    Article

    Benito-Kwiecinski et al Cell. 2021 Apr 15;184(8):2084-2102.e19.

    Insert

    CRE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tight TRE promoter

    Available since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. PPRE X3-TK-luc

    Plasmid

    #1015

    Purpose

    PPAR reporter. 3xDR1 sites upstream of a luciferase reporter.

    Depositor

    Bruce Spiegelman

    Article

    Kim et al Proc Natl Acad Sci U S A 1998 Apr 14;95(

    Insert

    PPRE
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SBE-Luc

    Plasmid

    #16527

    Depositor

    Bert Vogelstein

    Article

    Zhou et al Mol Cell. 1998 Jul . 2(1):121-7.

    Insert

    Smad binding element
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pCMV 3xFLAG-GABARAP WT

    Plasmid

    #123097

    Purpose

    Expresses 3xFLAG-GABARAP wild-type in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMOS028: Mitycam calcium sensor (mitochondrial)

    Plasmid

    #163046

    Purpose

    Mitycam calcium sensor (mitochondrial)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    Mitycam
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1_MMP-9-HA

    Plasmid

    #121172

    Purpose

    Expression of mouse MMP9

    Depositor

    Shinwu Jeong, M. Elizabeth Fini

    Article

    Jeong et al Am J Pathol. 2012 May;180(5):2028-39. do

    Insert

    MMP9 (matrix metallopeptidase 9) (Mmp9 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    CMV immediate/early

    Available since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEC3105 (pSpy1C-PxylS2_ffluc)

    Plasmid

    #218512

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporter

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Insert

    ffluc
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    deletion of Plac_mrfp cassette

    Promoter

    PxylS2

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato

    Plasmid

    #60658

    Purpose

    Flp-dependent expression of mNaChBac-T2A-tdTomato

    Depositor

    Massimo Scanziani

    Article

    Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

    Has Service

    AAV8

    Insert

    FLEX-mNaChBac-T2A-tdTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1α

    Available since

    Nov. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCBC-DT1T2

    Plasmid

    #50590

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Insert

    T1, U626t plus, U629p, T2
    

    Use

    CRISPR; Pcr template

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLminP_Luc2P_RE52

    Plasmid

    #90397

    Purpose

    STAT1 - STAT1 gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    STAT1

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge