159,258 results

1. pcDNA3.2/v5-DEST hGlut4

   Plasmid

   #18087

   Depositor

   Wolf Frommer

   Article

   Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

   Insert

   Glut4 (SLC2A4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-cTNT-Cre

   Plasmid

   #209785

   Purpose

   Expresses Cre by the specific cTNT promoter

   Depositor

   Yuxuan Guo

   Article

   Yang et al Circulation. 2024 May 28;149(22):1778-17

   Insert

   Cre
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   cTNT

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. STING-V1

   Plasmid

   #124262

   Purpose

   C-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments

   Depositor

   Eric Schirmer

   Insert

   TMEM173 (STING1 Human)
   

   Use

   Tags

   Venus1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDIRECT_22C

   Plasmid

   #91135

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:npt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. SF-SON IRES Tomato

   Plasmid

   #214403

   Purpose

   Expresses human SOX10, OLIG2, NKX6.2 and tdTomato

   Depositor

   Tanja Kuhlmann

   Article

   Ehrlich et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

   Insert

   SOX10, Olig2, NKX6.2 (SOX10, OLIG2, NKX6-2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   March 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-FLEX-RiboL1-jGCaMP8f

   Plasmid

   #192618

   Purpose

   AAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8f.

   Depositor

   Marianne Fyhn

   Article

   Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

   Insert

   FLEX-RiboL1-jGCaMP8f
   

   Use

   AAV and Cre/Lox

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. LeGO-Luc2

   Plasmid

   #154006

   Purpose

   Lentiviral vector expressing firefly luciferase (Luc2)

   Depositor

   Boris Fehse

   Article

   Ellinger et al Viruses. 2020 May 25;12(5). pii: v120505

   Insert

   Firefly Luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHCMV_RV_G

   Plasmid

   #207303

   Purpose

   Mammalian expression of Rabies virus G protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   RV_G (RABVgp4 Rabies virus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMD2.G-VSV-G-mut

   Plasmid

   #196508

   Purpose

   VSV-G mutant (K47Q, R354A)

   Depositor

   Howard Chang

   Article

   Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

   Insert

   VSV-G
   

   Use

   Tags

   NA

   Expression

   Mammalian

   Mutation

   K47Q, R354A

   Promoter

   CMV

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP_CD47_SU

    Plasmid

    #65473

    Purpose

    GFP tagged CD47 with its own short 3'UTR

    Depositor

    Christine Mayr

    Article

    Berkovits et al Nature. 2015 Apr 20. doi: 10.1038/nature

    Inserts

    Signal peptide of CD47
    GFP
    CD47 coding region continued and short 3’UTR.
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAVS1-Tet-OsTIR1(F74G)-V5

    Plasmid

    #158664

    Purpose

    Tet-OsTIR1(F74G)-V5 donor for integration at the AAVS1 locus

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    Tet-OsTIR1(F74G) AAVS1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    F74G

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHSE401

    Plasmid

    #62201

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    gRNA scaffold
    zCas9
    

    Use

    Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    maize codon optimized

    Promoter

    35S and AtU6-26p

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. Mut1-RS

    Plasmid

    #182881

    Purpose

    A pEvol-based plasmid containing one inducible (by arabinose) copy of the Mut1-RS aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.

    Depositor

    Miriam Amiram

    Article

    Hadar et al Chembiochem. 2021 Apr 16;22(8):1379-1384

    Insert

    Mut1-RS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAG423GPD-ccdB

    Plasmid

    #14150

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. dCas9-miniTurbo

    Plasmid

    #219822

    Purpose

    EF1α-driven expression of dCas9 fused to miniTurbo with GFP (Adapted from plasmid #153209)

    Depositor

    Philipp Maass

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    miniTurbo

    Expression

    Mammalian

    Mutation

    D10A/H841A

    Promoter

    EF1a

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. BCL2L1_pLX307

    Plasmid

    #98323

    Purpose

    Lentiviral expression of BCL2L1

    Depositor

    Sefi Rosenbluh, William Hahn

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    BCL2L1 (BCL2L1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. HA PKB T308D S473D pcDNA3

    Plasmid

    #14751

    Depositor

    Jim Woodgett

    Article

    Scheid et al Mol Cell Biol. 2002 Sep . 22(17):6247-60

    Insert

    PKB DD (AKT1 Bovine)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T308D S473D. Constitutively active mutant.

    Promoter

    Available since

    April 12, 2007

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pCX420

    Plasmid

    #229211

    Purpose

    Cytoplasmic-targeting Csm complex plasmid

    Depositor

    Jennifer Doudna

    Article

    Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

    Insert

    Csm1; Csm2; Csm3(RNase mut)-2xsfGFP; Csm4; Csm5; Cas6
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAR17_StrepI_hFASh_H8_pET22b

    Plasmid

    #122846

    Purpose

    Expresses human type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.

    Depositor

    Martin Grininger

    Article

    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

    Insert

    human FASN/ human fatty acid synthase (FASN Human)
    

    Use

    Tags

    His8 tag and Twin-Strep tag

    Expression

    Bacterial

    Mutation

    wildtype

    Promoter

    T7 promoter

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCW57.1 N-term GFP tTA

    Plasmid

    #107551

    Purpose

    All-in-one dox-repressible expression of cDNA with optional N-term GFP tag.

    Depositor

    David Sabatini

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    GFP (optional)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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