159,258 results

1. pXR003: CasRx gRNA cloning backbone

   Plasmid

   #109053

   Purpose

   hU6-driven expression of guide RNAs compatible with CasRx. 5' processed DR followed by BbsI sites for guide cloning.

   Depositor

   Patrick Hsu

   Article

   Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. HA-Sos1

   Plasmid

   #32920

   Depositor

   Dafna Bar-Sagi

   Article

   Yang et al J Biol Chem. 1995 Aug 4;270(31):18212-5.

   Insert

   SOS1 (SOS1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. NLS-mCherry-LEXY (pDN122)

   Plasmid

   #72655

   Purpose

   Blue light-inducible nuclear export of mCherry

   Depositor

   Barbara Di Ventura, Roland Eils

   Article

   Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

   Insert

   NLS-mCherry-LEXY
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40

   Plasmid

   #100852

   Purpose

   AAV expression of Cre recombinase-activated jRGECO1a, a red fluorescent calcium sensor protein, from the CAG promoter

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Has Service

   AAV1

   Insert

   jRGECO1a
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. CL20_mEGFP_CBFB_MYH11

   Plasmid

   #205787

   Purpose

   Express mEGFP-tagged fusion protein, CBFB_MYH11 from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   CBFB_MYH11 (CBFB, MYH11 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLPT107

   Plasmid

   #85525

   Purpose

   Repressilator with triple reporter

   Depositor

   Johan Paulsson

   Article

   Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

   Inserts

   mCfp
   mVenus
   tetR
   lacI
   cI
   mKate2
   

   Use

   Synthetic Biology

   Tags

   ssrA

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. myc-KIF5B

   Plasmid

   #127617

   Purpose

   N-terminal myc-tagged mouse KIF5B

   Depositor

   Josef Kittler

   Article

   Twelvetrees et al Neuron. 2010 Jan 14;65(1):53-65. doi: 10

   Insert

   KIF5B (Kif5b Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT7-7 asyn E46K

   Plasmid

   #105728

   Purpose

   Bacterial expression of mutant human alpha synuclein

   Depositor

   Hilal Lashuel

   Article

   Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

   Insert

   alpha-synuclein E46K (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E46K

   Promoter

   T7

   Available since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHSN6I01

   Plasmid

   #50587

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   dCas9-KRAB
   gRNA scaffold
   

   Use

   CRISPR; Plant expression

   Tags

   3xFLAG, HA, and NLS

   Expression

   Mutation

   dCas9 that is defective in DNA cleavage; the maiz…

   Promoter

   2×35Sp and AtU6-26p

   Available since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-hARΔ142–337

    Plasmid

    #89106

    Purpose

    Mammalian expression of human androgen receptor with deletion of amino acids 142-337 N-terminal transactivation function 1

    Depositor

    Elizabeth Wilson

    Article

    Titus et al PLoS One. 2012;7(1):e30192. doi: 10.1371

    Insert

    hAR delta 142-337 (AR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hAR delta 142-337

    Promoter

    CMV

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

11. pINDUCER21-ERG

    Plasmid

    #51301

    Purpose

    Expression of ERG after doxycycline administration

    Depositor

    George Daley

    Article

    Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

    Insert

    v-ets avian erythroblastosis virus E26 oncogene homolog (ERG Human)
    

    Use

    Lentiviral

    Tags

    n/a

    Expression

    Mutation

    full length ORF

    Promoter

    Available since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV hSYN DIO mGluR1-myc-FLAG

    Plasmid

    #166227

    Purpose

    AAV expressing cre-dependent mGluR1-myc-FLAG

    Depositor

    Jason Aoto

    Article

    Cai et al Neuron. 2021 Apr 7;109(7):1137-1149.e5.

    Insert

    metabotropic glutamate receptor 1 (Grm1 Mouse)
    

    Use

    AAV and Cre/Lox

    Tags

    Myc and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCerulean

    Plasmid

    #24756

    Depositor

    Martin Fraunholz

    Article

    Paprotka et al J Microbiol Methods. 2010 Aug 10. ():.

    Insert

    Cerulean
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pCMV-hARdelta120-472

    Plasmid

    #89088

    Purpose

    mammalian expression of human androgen receptor with deletion of 120-472 amino acid residues

    Depositor

    Elizabeth Wilson

    Article

    Bai et al Mol Cell Biol. 2008 Mar;28(6):1947-63. d

    Insert

    hAR-120-472 N-term deletion (AR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hAR-120-472

    Promoter

    CMV

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5/FRT-HA-hM3D(Gq)

    Plasmid

    #45547

    Purpose

    Expresses hM3D (Gq) neuronal activator

    Depositor

    Bryan Roth

    Article

    Armbruster et al Proc Natl Acad Sci U S A. 2007 Mar 20;10

    Insert

    hM3D (Gq)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 PRDM16

    Plasmid

    #15503

    Depositor

    Bruce Spiegelman

    Article

    Seale et al Cell Metab. 2007 Jul . 6(1):38-54.

    Insert

    PRDM16 (Prdm16 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. ML1Nwt_in_pEGFP-C1

    Plasmid

    #67797

    Purpose

    Mammalian expression vector driving Mouse ML1Nwt (2xML1N domain wildtype version) fused to EGFP

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    ML1N*2-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRDA_887

    Plasmid

    #201164

    Purpose

    Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65 and NLS

    Depositor

    John Doench

    Article

    Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

    Insert

    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHCMV_DENV1_PrME

    Plasmid

    #207263

    Purpose

    Mammalian expression of Dengue virus 1 PrME protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    DENV1_PrME (POLY Dengue virus 1)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAGD335

    Plasmid

    #219499

    Purpose

    pAGD248 derivative with the retron Eco1 msr, sfGFP flanked by 2 BsaI sites , and the rest of the retron recombineering operon (Eco1 RT, CspRect, MutL-E32K) downstream of the pBad promoter

    Depositor

    Seth Shipman

    Article

    Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

    Insert

    Eco1 msr, sfGFP, Eco1 RT, CspRecT, MutLE32K
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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