161,600 results

1. pLenti Lifeact-mRuby2 PuroR

   Plasmid

   #84386

   Purpose

   Lentiviral expression of mRuby2-tagged Lifeact with Puromycin selection in cells including neurons

   Depositor

   Ghassan Mouneimne

   Insert

   Lifeact
   

   Use

   Lentiviral

   Tags

   mRuby2

   Expression

   Mutation

   Promoter

   CMV immediate early

   Available since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. mAID-BRD4 donor

   Plasmid

   #140650

   Purpose

   BRD4 tagging with mAID

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   BRD4 (BRD4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-Kir2.1WT-2A-mScarlet-KASH

   Plasmid

   #203840

   Purpose

   Kir2.1 expression for silencing neurons and mScarlet-KASH expression

   Depositor

   Ivo Spiegel

   Article

   Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

   Insert

   Kir2.1 (Kcnj2 Mouse)
   

   Use

   AAV

   Tags

   2A-mScarlet-KASH

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pC1-CMV-DAAO-NES

   Plasmid

   #141107

   Purpose

   Expresses DAAO with nuclear export signal in mammalian cells

   Depositor

   Vsevolod Belousov

   Article

   Bogdanova et al Curr Protoc Chem Biol. 2017 Jun 19;9(2):

   Insert

   DAAO-NES
   

   Use

   Tags

   Nuclear export signal

   Expression

   Mammalian

   Mutation

   deleted DAAO amino acids 367-368

   Promoter

   CMV

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. p5E-mpeg1.1

   Plasmid

   #75023

   Purpose

   Can be used to drive expression in zebrafish microglia.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Insert

   macrophage expressed 1, tandem duplicate 1 (mpeg1.1 Zebrafish)
   

   Use

   5' entry vector for multisite gateway three …

   Tags

   Expression

   Mutation

   Promoter

   mpeg1.1

   Available since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKS1

   Plasmid

   #84079

   Purpose

   Expresses the non-toxic fragment C of tetanus toxin

   Depositor

   Neil Fairweather

   Article

   Sinha et al Mol Microbiol. 2000 Sep;37(5):1041-51.

   Insert

   Tetanus neurotoxin fragment C
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-hMC4R

   Plasmid

   #179315

   Purpose

   Express human melanocortin 4 receptor (GFP tagged) in mammalian cells

   Depositor

   Christian Gruber

   Article

   Muratspahic et al ACS Pharmacol Transl Sci. 2023 Sep 26;6(

   Insert

   melanocortin 4 receptor (MC4R Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro

   Plasmid

   #73022

   Purpose

   Luminescent and fluorescent dual reporter

   Depositor

   Kazuhiro Oka

   Insert

   Nluc-P2A-copGFP-T2A-Puro
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1

   Available since

   Jan. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3/UBC9

   Plasmid

   #20082

   Depositor

   Edward Yeh

   Article

   Gong et al J Biol Chem. 1997 Nov 7;272(45):28198-20

   Insert

   UBC9 (UBE2I Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-DN-hCUL4B-FLAG

    Plasmid

    #15822

    Depositor

    Wade Harper

    Article

    Jin et al Methods Enzymol. 2005 . 399():287-309.

    Insert

    Cullin4B (CUL4B Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Dominant negative: N594

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIGneo

    Plasmid

    #52116

    Purpose

    a bicistronic IRES-GFP containing vector for mammalian expression (CMV promoter)

    Depositor

    Dario Vignali

    Type

    Empty backbone

    Use

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-DN-hCUL5-FLAG

    Plasmid

    #15823

    Depositor

    Wade Harper

    Article

    Jin et al Methods Enzymol. 2005 . 399():287-309.

    Insert

    Cullin5 (CUL5 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Dominant negative: N441

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS2+-eGFP

    Plasmid

    #221710

    Purpose

    Expression of eGFP in zebrafish embryos.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    eGFP
    

    Use

    Fish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-Kir2.1-T2A-tdTomato

    Plasmid

    #60598

    Purpose

    Expresses Kir2.1-T2A-tdTomato

    Depositor

    Massimo Scanziani

    Article

    Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

    Insert

    Kir2.1-T2A-tdTomato (Kcnj2 Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pXR001: EF1a-CasRx-2A-EGFP

    Plasmid

    #109049

    Purpose

    CasRx (NLS-RfxCas13d-NLS) with 2A-EGFP for RNA targeting

    Depositor

    Patrick Hsu

    Article

    Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

    Insert

    CasRx
    

    Use

    CRISPR and Lentiviral

    Tags

    2A-eGFP, HA, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    May 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYPQ223 (dAsCpf1-SRDX)

    Plasmid

    #86209

    Purpose

    Deactivated AsCpf1-SRDX repressor fusion; transcriptional repressor dCpf1 Gateway entry plasmid

    Depositor

    Yiping Qi

    Article

    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

    Insert

    dAsCpf1
    

    Use

    Gateway compatible cpf1 vectors

    Tags

    NLS and SRDX repressor and NLS

    Expression

    Plant

    Mutation

    D908A

    Promoter

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRISPomyces-2

    Plasmid

    #61737

    Purpose

    Streptomyces expression of codon-optimized Cas9 and custom gRNA

    Depositor

    Huimin Zhao

    Article

    Cobb et al ACS Synth Biol. 2014 Dec 8.

    Inserts

    sSpCas9
    gRNA cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    BbsI-flanked lacZ cassette inserted in place of s…

    Promoter

    gapdhp(EL) and rpsL(XC)-BbsI

    Available since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-DN-hCUL2-FLAG

    Plasmid

    #15819

    Depositor

    Wade Harper

    Article

    Jin et al Methods Enzymol. 2005 . 399():287-309.

    Insert

    Cullin2 (CUL2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Dominant negative: N427

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. p8.91

    Plasmid

    #187441

    Purpose

    Lentiviral packaging plasmid expressing gag and pol.

    Depositor

    Simon Davis

    Article

    Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

    Inserts

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pC0046-EF1a-PspCas13b-NES-HIV

    Plasmid

    #103862

    Purpose

    Expresses PspCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    PspCas13b
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and HIV NES

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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