165,652 results

1. pCRISPRi_Mxi1_yl_NHEJ

   Plasmid

   #91249

   Purpose

   CRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HR

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

   Inserts

   Codon optimized dCas9-Mxi1
   KU70 sgRNA expression cassette
   KU80a sgRNA expression cassette
   KU80b sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Promoter

   SCR1'-tRNA and UAS1B8-TEF(136)

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF2815

   Plasmid

   #144244

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   FOSB (FOSB Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. BB3cN_pGAP_23*_pLAT1_Cas9

   Plasmid

   #104906

   Purpose

   hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC

   Depositor

   Brigitte Gasser

   Article

   Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

   Inserts

   HH - FS23 linker - HDV
   human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Feb. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Ef1a_Large T-antigen_Ires_Puro

   Plasmid

   #18922

   Depositor

   Linzhao Cheng

   Article

   Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

   Insert

   Large T Antigen (SV40gp6 Simian virus 40)
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 4, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA6a-link-yoSuperfolderGFP-Kan

   Plasmid

   #44901

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized SuperfolderGFP with Kan selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoSuperfolderGFP
   

   Expression

   Yeast

   Available Since

   May 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-28a_6H-TAQ_E602D

   Plasmid

   #166944

   Purpose

   Bacterial expression of Taq DNA polymerase

   Depositor

   Robert Tjian

   Article

   Graham et al PLoS One. 2021 Feb 3;16(2):e0246647. doi

   Insert

   Thermus aquaticus DNA polymerase
   

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   E602D

   Promoter

   T7

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3-SREBP-1-GFP (A317-318; A341)

   Plasmid

   #204198

   Purpose

   Mammalian expression of SREBP-1 (isoform 1)

   Depositor

   Allan Weissman

   Article

   Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

   Insert

   SREBP-1 (SREBF1 Human)
   

   Tags

   3xHA, eGFP and T7x2

   Expression

   Mammalian

   Mutation

   Gly to Ala 317,318,341

   Available Since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pC3.1_CMV_oROS-G

   Plasmid

   #216111

   Purpose

   Expresses the genetically encoded green fluorescent hydrogen peroxide sensor in mamalian cells.

   Depositor

   Andre Berndt

   Article

   Lee et al bioRxiv 2024.01.31.578117

   Insert

   oROS-G
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. GW1-rogRFP2

   Plasmid

   #207876

   Purpose

   Mammalian expression of the red fluorescent rogRFP2 redox sensor

   Depositor

   Mathew Tantama

   Article

   Radhakrishnan et al ACS Chem Neurosci. 2020 Sep 2;11(17):266

   Insert

   rogRFP2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNAKv10.1mVenus

    Plasmid

    #85705

    Purpose

    Kv10.1 fused mVenus

    Depositor

    Luis Pardo

    Article

    Chen et al PLoS One. 2011 May 3;6(5):e19257. doi: 1

    Insert

    KCNH1 (KCNH1 Human)
    

    Tags

    mVenus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-Luc

    Plasmid

    #45968

    Depositor

    Ralf Kuehn

    Insert

    Luciferase
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMOS008E: Entry vector for: GCaMP6F calcium sensor (cytosolic)

    Plasmid

    #163064

    Purpose

    Entry vector for: GCaMP6F calcium sensor (cytosolic)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    GCaMP6F
    

    Use

    Gateway entry vector

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC18-mini-Tn7

    Plasmid

    #64953

    Purpose

    mini-Tn7 base vector

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Aug. 14, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pDM1081

    Plasmid

    #108981

    Purpose

    Knockout cassette Hygromycin

    Depositor

    Rob Kay

    Article

    Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

    Insert

    Knockout cassette Hygromycin
    

    Use

    Cre/Lox; Dictyostelium discoideum knockout vector

    Promoter

    coaA

    Available Since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. TFORF3192

    Plasmid

    #144668

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ERG (ERG Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. mTagBFP2-CD151-7

    Plasmid

    #55282

    Purpose

    Localization: Tetraspanin/Membrane, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    CD151 (CD151 Human)
    

    Tags

    mTagBFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 7, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pFUW-tetO-Relb

    Plasmid

    #139810

    Purpose

    doxycycline-inducible expression of mouse Relb in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    Relb (Relb Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE-mCMV

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. myr-palm-mCherry2

    Plasmid

    #197910

    Purpose

    Monomer expression vector with plasma membrane localization signal

    Depositor

    Salvatore Chiantia

    Article

    Petrich et al Biophys J. 2021 Dec 21;120(24):5478-5490

    Insert

    myristoylated-palmitoylated-mCherry2
    

    Expression

    Mammalian

    Mutation

    none

    Available Since

    July 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-LSSA12

    Plasmid

    #186545

    Purpose

    LSSA12 fluorescent protein (ex/em = 398/511 nm): cytosolic expression in mammalian cells

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    LSSA12
    

    Expression

    Mammalian

    Mutation

    hfYFP-G65S/Y203I/Q204E/E222D/R223F

    Promoter

    CMV

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1 - BirA* (AA141-321) - HA - NIPP1

    Plasmid

    #86885

    Purpose

    C-terminal part of the split-BioID

    Depositor

    Mathieu Bollen

    Article

    Munter et al FEBS Lett. 2016 Dec 29. doi: 10.1002/187

    Insert

    BirA* (AA141-321)- HA- NIPP1]
    

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    BirA Amino Acids 141-321

    Available Since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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