163,486 results

1. pLSB-KAN

   Plasmid

   #166700

   Purpose

   Combined sgRNA/Cas9 pLSB vector with kanMX6 (G418) marker. Golden Gate-ready for cloning custom sgRNA sequences.

   Depositor

   Robin Allshire

   Article

   Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do

   Inserts

   tRNA promoter : sgRNA cassette
   adh15 promoter : Cas9 codon-optimised for S. pombe
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   adh15 promoter and tRNA Ser promoter

   Available Since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1-GGamma9-GFP2

   Plasmid

   #140991

   Purpose

   Encodes a G gamma subunit (GNG9) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma9-GFP2 (GNGT2 Human)
   

   Use

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-PE6f

   Plasmid

   #207856

   Purpose

   Mammalian expression of PE6f prime editor

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   PE6f
   

   Use

   Tags

   SV40 bpNLS and SV40 bpNLS, c-Myc NLS

   Expression

   Mammalian

   Mutation

   Cas9(H840A, E471K, H99R, I632V, H721Y, D645N, K91…

   Promoter

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pT3TS-spCas9 ABE-Umax-ex2-rest1

   Plasmid

   #222143

   Purpose

   Adenine base editor with modified TadA variant for extended window and restricted A-to-G base editing in zebrafish

   Depositor

   Gaurav Varshney

   Article

   Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

   Insert

   Modified TadA-nSpCas9
   

   Use

   CRISPR; Zebrafish expression

   Tags

   Bipartite NLS

   Expression

   Mutation

   Promoter

   T3

   Available Since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. EF06R

   Plasmid

   #42940

   Depositor

   Eline Luning Prak

   Article

   Farkash et al Nucleic Acids Res. 2006 Feb 28;34(4):119

   Insert

   Human L1 element (L1RE1 Human)
   

   Use

   Tags

   EGFP (opposite strand)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV MIE, L1 5'UTR

   Available Since

   April 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. elavl3:hArr-TEV;he1.1:YFP

   Plasmid

   #218855

   Purpose

   D. rerio elavl3 promoter driving expression of human Arrestin-TEV protease fusion protein for use in trans-Tango.

   Depositor

   Marnie Halpern

   Article

   Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

   Insert

   hArr
   

   Use

   Tags

   human Arrestin-Tobbaco Etch Virus protease fusion…

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. lentiCRISPRv2 blast

   Plasmid

   #98293

   Purpose

   Variant of lentiCRISPRv2 that confers blasticidin S resistance

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF-1a

   Available Since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1_nV5-AMOTL1

   Plasmid

   #172998

   Purpose

   constitutive expression of V5-tagged AMOTL1

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   V5-AMOTL1 (AMOTL1 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ABE9

   Plasmid

   #194208

   Purpose

   Mammalian expression of ABE9

   Depositor

   Dali Li

   Article

   Chen et al Nat Chem Biol. 2022 Oct 13. pii: 10.1038

   Insert

   TadA-8e (N108Q/L145T)-nSpCas9
   

   Use

   CRISPR

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV hSyn FLEx-loxp Kir2.1-2A-GFP

    Plasmid

    #161574

    Purpose

    Expression of Kir2.1-2A-GFP in a Cre-dependent fashion

    Depositor

    Kevin Beier, Robert Malenka

    Article

    Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

    Insert

    Kir2.1-2A-GFP (Kcnj2 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available Since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pT7-EGFP-C1-HsXRN1_K

    Plasmid

    #146784

    Purpose

    Mammalian Expression of HsXRN1

    Depositor

    Elisa Izaurralde

    Article

    Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

    Insert

    HsXRN1 (XRN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSpCas9n(BB)-2A-Puro (PX462) V2.0

    Plasmid

    #62987

    Purpose

    Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Promoter

    Available Since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PB-TAC-ERP2-(N-HA)KRAS_G12D

    Plasmid

    #201254

    Purpose

    Dox-inducible overexpression of HA-tagged KRASG12D and mCherry in human cells

    Depositor

    Alexander Kleger

    Article

    Breunig et al Cell Stem Cell. 2021 Jun 3;28(6):1105-11

    Insert

    KRAS (KRAS Human)
    

    Use

    Piggybac transposon

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    changed Gly (G) 12 to Asp (D)

    Promoter

    TRE

    Available Since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0

    Plasmid

    #174081

    Purpose

    Expression of orthogonal Mb tRNA and tRNA synthetase for incorporation of tetrazine3.0 in eukaryotic cells.

    Depositor

    Ryan Mehl

    Article

    Jang et al J Am Chem Soc. 2020 Apr 22;142(16):7245-

    Insert

    NES-Flag-R2-84 M. barkeri tRNA synthetase for tetrazine3.0
    

    Use

    Tags

    NES-Flag

    Expression

    Mammalian

    Mutation

    L270G, Y271L, N311G, C313A

    Promoter

    CMV

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFLAG-CMV-hErk1

    Plasmid

    #49328

    Purpose

    Expresses FLAG-tagged human Erk1

    Depositor

    Melanie Cobb

    Insert

    Erk1 (MAPK3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. STING-miRFP680

    Plasmid

    #200940

    Purpose

    STING fused with miRFP680 for live cell imaging

    Depositor

    Nir Hacohen

    Article

    Liu et al Science. 2023 Aug 4;381(6657):508-514. d

    Inserts

    STING (STING1 Human)
    miRFP680
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-ADAM10-HA

    Plasmid

    #65106

    Purpose

    Expression of HA tagged ADAM10 in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

    Insert

    ADAM10 (ADAM10 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTRKH3-ermGFP

    Plasmid

    #27169

    Depositor

    Michela Lizier

    Article

    Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

    Insert

    erythromycin ribosomal methylase promoter region fused with modified green fluorescent protein 5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    presence of an additional 183-bp region at N term…

    Promoter

    Available Since

    Feb. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAVS1-PDi-CRISPRn

    Plasmid

    #73500

    Purpose

    Dox-inducible CRISPR nuclease (CRISPRn) knock in construct into the AAVS1 locus

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Inserts

    Cas9
    rtTA
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and TRE

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CAG-mCherry

    Plasmid

    #108685

    Purpose

    Strong CAG promoter with mCherry expression for transfection efficacy screening

    Depositor

    Jordan Green

    Article

    Mishra et al Regen Eng Transl Med. 2019 Sep;6(3):273-

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge