We narrowed to 3,355 results for: phage

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pCAG-Beatrix

Plasmid

#167528

Purpose

Dual-color fluorescent reporter for the creation and detection of Cre-dependent tunable mosaics in floxed mouse lines and cells.

Depositor

Gian Michele Ratto

Article

Trovato et al Nat Commun. 2020 Dec 3;11(1):6194. doi:

Insert

Beatrix

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Promoter

CAG

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-αIFNα-ab

Plasmid

#110799

Purpose

Expression and secretion of an scFv antibody (clone) 4EA1 against all mouse interferon α subtypes in mammalian cells

Depositor

Thomas Böldicke

Article

Bussow et al PLoS One. 2019 Apr 16;14(4):e0215062. do

Insert

antibody against mouse interferon alpha

Tags

ER signal peptide, His6 tag, and myc epitope tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-αIFNα-ib

Plasmid

#110798

Purpose

Expression of an intrabody derived from the monoclonal antibody 4EA1 against all mouse interferon α subtypes in mammalian cells

Depositor

Thomas Böldicke

Article

Bussow et al PLoS One. 2019 Apr 16;14(4):e0215062. do

Insert

intrabody against mouse interferon alpha

Tags

ER retention signal (SEKDEL), ER signal peptide, …

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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BE3-P2A-EGFP (pJUL977)

Plasmid

#123612

Purpose

CAG promoter expression plasmid for rAPOBEC1-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP.

Depositor

Keith Joung

Article

Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

Insert

BE3-P2A-EGFP

Expression

Mammalian

Mutation

D10A in SpCas9

Promoter

CAG

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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eA3A-BE3 (pRZ206)

Plasmid

#131315

Purpose

CAG promoter expression plasmid for hA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

Insert

hA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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hA3A-BE3 (pRZ215)

Plasmid

#131314

Purpose

CAG promoter expression plasmid for hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

Insert

hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pMCSF-R(DD)-luc

Plasmid

#12424

Depositor

Dong-Er Zhang

Article

Zhang et al Mol Cell Biol. 1994 Dec . 14(12):8085-95

Insert

M-CSF receptor promoter fragment (CSF1R Human)

Use

Luciferase

Mutation

Deletion from bp-86 to -37 (created a KpnI site i…

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
pCMV/myc/ER-αT2ib

Plasmid

#110769

Purpose

Expresses ER intrabody against mouse and human TLR2

Depositor

Thomas Böldicke

Article

Kirschning et al BMC Biotechnol. 2010 Apr 13;10:31. doi:

Insert

intrabody against mouse and human TLR2

Tags

ER retention signal (SEKDEL), ER signal peptide, …

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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hAID-BE3 (pRZ352)

Plasmid

#131316

Purpose

CAG promoter expression plasmid for hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

Insert

hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pMCSF-R(mA)-luc

Plasmid

#12421

Depositor

Dong-Er Zhang

Article

Zhang et al Mol Cell Biol. 1996 Mar . 16(3):1231-40.

Insert

M-CSF receptor promoter fragment (CSF1R Human)

Use

Luciferase

Mutation

C/EBP binding site mutation (created a KpnI site …

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-FRET_PST-C

Plasmid

#228570

Purpose

Expressed a FRET sensor for determining proteolytic cleavage of the C-terminal side of Pancreastatin

Depositor

Geert van den Bogaart

Article

Ioannidis et al J Endocr Soc. 2025 May 15;9(7):bvaf090.

Insert

Chromogranin-A (CHGA Human)

Tags

mCitrine and mScarlet-I

Expression

Mammalian

Mutation

FRET sensor; see explanatory note

Promoter

cmv

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FRET_PST-N

Plasmid

#228571

Purpose

Expressed a FRET sensor for determining proteolytic cleavage of the N-terminal side of Pancreastatin

Depositor

Geert van den Bogaart

Article

Ioannidis et al J Endocr Soc. 2025 May 15;9(7):bvaf090.

Insert

Chromogranin-A (CHGA Human)

Tags

mCitrine and mScarlet-I

Expression

Mammalian

Mutation

FRET sensor; see explanatory note

Promoter

cmv

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FRET_CST-C

Plasmid

#228572

Purpose

Expressed a FRET sensor for determining proteolytic cleavage of the C-terminal side of Catestatin

Depositor

Geert van den Bogaart

Article

Ioannidis et al J Endocr Soc. 2025 May 15;9(7):bvaf090.

Insert

Chromogranin-A (CHGA Human)

Tags

mCitrine and mScarlet-I

Expression

Mammalian

Mutation

FRET sensor; see explanatory note

Promoter

cmv

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FRET_CST-N

Plasmid

#228573

Purpose

Expressed a FRET sensor for determining proteolytic cleavage of the N-terminal side of Catestatin

Depositor

Geert van den Bogaart

Article

Ioannidis et al J Endocr Soc. 2025 May 15;9(7):bvaf090.

Insert

Chromogranin-A (CHGA Human)

Tags

mCitrine and mScarlet-I

Expression

Mammalian

Mutation

FRET sensor; see explanatory note

Promoter

cmv

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet-MerTK_KRmutant-PTPN1

Plasmid

#196447

Purpose

Expression of MerTK Kinase Domain in E. coli and surface entropy reduction mutant for crystallization, coexpress PTPN1

Depositor

Byung Il Lee

Article

Bae et al Exp Mol Med. 2022 Sep;54(9):1450-1460. d

Inserts

Tyrosine-Protein Kinase Mer (MERTK Human)
PTPN1 (PTPN1 Human)

Expression

Bacterial

Mutation

K591R, K639R, K702R, K865R

Promoter

T7 promoter

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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psgRNA-2xMS2-lacO

Plasmid

#181909

Purpose

Single guide RNA with 2XMS2 loops targeting bacterial lac operator

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

sgRNA-2XMS2-lacO

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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psgRNA-2xMS2-mSAT

Plasmid

#181908

Purpose

Single guide RNA with 2XMS2 loops targeting mouse major satellite repeats

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

sgRNA-2XMS2-mSAT

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-IDO1-p2a-eGFP

Plasmid

#187026

Purpose

Expresses IDO1 and GFP (seperately) in mammalian cells

Depositor

Geert van den Bogaart

Article

Maassen et al Free Radic Biol Med. 2022 Aug 1;188:287-

Insert

Indoleamine 2,3-dioxygenase 1 (IDO1 Human)

Tags

P2A-GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pLex307-UCOE-SFFV-puroR-MCP-HSF1-VIRF2

Plasmid

#218557

Purpose

Encodes SFFV promoter-driven MCP-HSF1-VIRF2 with an N-terminal bipartite SV40 NLS, along with puromycin resistance

Depositor

Alejandro Chavez

Article

Giddins et al Nat Commun. 2025 Nov 20;16(1):11114. doi

Insert

MCP-HSF1-VIRF2

Use

Lentiviral

Tags

SV40NLS

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pBFC0984

Plasmid

#231992

Purpose

CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with 2xBsaI spacer cloning site

Depositor

Brady Cress

Article

Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d

Inserts

dRfxCas13d
crRNA with 2xBsaI spacer

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119 and pTet

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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