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Showing: 301 - 320 of 9101 results

  1. pCSDest2-2XNLS-SpCas9-WT-NLS-3XHA-NLS-TALentry

    Plasmid
    #69232
    Purpose
    Wild type SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)
    Depositor
    Scot Wolfe
    Article
    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n
    Insert
    SpCas9
    Use
    CRISPR
    Tags
    2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    69232-plasmid-map-sequence-id-122311

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  5. pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo

    Plasmid
    #183411
    Purpose
    piggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.
    Depositor
    Azim Surani
    Article
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Insert
    Tet-on 3G
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1A
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183411-plasmid-map-sequence-id-361257

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  9. pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALentry

    Plasmid
    #69233
    Purpose
    R1335K mutant SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)
    Depositor
    Scot Wolfe
    Article
    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n
    Insert
    SpCas9
    Use
    CRISPR
    Tags
    2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…
    Expression
    Mammalian
    Mutation
    Arginine 1335 is changed to Lysine
    Promoter
    Available since
    Nov. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    69233-plasmid-map-sequence-id-122312

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  17. pRosetta

    Plasmid
    #59700
    Purpose
    Lentiviral vector for use as a standard for titering experiments when trying to compare vectors with different selection markers or to determine appropriate antibiotic conditions.
    Depositor
    David Root, John Doench
    Inserts
    Puro
    BlastR
    EGFP
    Use
    Lentiviral
    Tags
    2A
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    59700-plasmid-map-sequence-id-140560

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  18. pHEE2E-TRI

    Plasmid
    #71288
    Purpose
    Egg cell-specific promoter-controlled expression 3×FLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, TRY, and CPC
    Depositor
    Qi-Jun Chen
    Article
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    sgRNA targeting TRY and CPC genes
    sgRNA targeting ETC2 gene
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71288-plasmid-map-sequence-id-166343

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Showing: 301 - 320 of 9101 results