Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 4,853 results for: codon optimized

Clear keywords
Showing: 3481 - 3500 of 4853 results

  1. pLenti-nSpCas9-BEACON2

    Plasmid
    #209041
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where BEACON2 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Publication
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    BEACON2-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209041-plasmid-map-sequence-id-419488

    Enlarge

  2. pYPQ289-RVR

    Plasmid
    #138127
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized ErCas12a with D513R, K519V and N523R mutations, without promoter
    Depositor
    Yiping Qi
    Publication
    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:
    Insert
    ErCas12a RVR variant
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    D513R, K519V and N523R
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    138127-plasmid-map-sequence-id-272088

    Enlarge

  3. pYPQ284-v1

    Plasmid
    #138118
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with D172R mutation, without promoter
    Depositor
    Yiping Qi
    Publication
    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:
    Insert
    Mb2Cas12a v1
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    D172R
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    138118-plasmid-map-sequence-id-272075

    Enlarge

  4. pYPQ223-STU

    Plasmid
    #138109
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized, catalytically deactivated AsCas12a-SRDX repressor without promoter and terminator
    Depositor
    Yiping Qi
    Publication
    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:
    Insert
    dAsCas12a-SRDX
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    NLS
    Expression
    Plant
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    138109-plasmid-map-sequence-id-272024

    Enlarge

  5. pCJ206

    Plasmid
    #162679
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating the E226T mutation to the putative lipase box.
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), incorporating the E226T mutation to the putative lipase box
    Tags
    His
    Expression
    Bacterial
    Mutation
    E226T; codon optimized for expression in E. coli …
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    162679-plasmid-map-sequence-id-321456

    Enlarge

  6. pCJ197

    Plasmid
    #162670
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S131G mutation.
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1
    Tags
    His
    Expression
    Bacterial
    Mutation
    S131G; codon optimized for expression in E. coli …
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    162670-plasmid-map-sequence-id-321452

    Enlarge

  9. pAAV-CaMKII-Cx34.7-M1-2xHA-T2A-mEmerald

    Plasmid
    #255636
    Purpose
    Encodes M. americana Cx34.7, codon optimized for mouse expression with c-terminally fused 2x hemagglutinin (HA) tag, T2A self-cleaving peptide and mEmerald. Mutations E214K and E223K. CaMKII promoter.
    Depositor
    Kafui Dzirasa
    Publication
    Ransey et al Nature. 2026 May 13. doi: 10.1038/s41586
    Insert
    Cx34.7
    Use
    AAV
    Tags
    2xHA-T2A-mEmerald
    Mutation
    E214K,E223K
    Promoter
    CaMKII
    Available since
    June 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    255636-plasmid-map-sequence-id-512735

    Enlarge

  12. pAAV-CaMKII-Cx34.7-M1-mEmerald

    Plasmid
    #255632
    Purpose
    Encodes M. americana Cx34.7, codon optimized for mouse expression, with 8 amino acid linker and c-terminally fused mEmerald tag. Mutations of E214K and E223K. Expression driven by CaMKII promoter.
    Depositor
    Kafui Dzirasa
    Publication
    Ransey et al Nature. 2026 May 13. doi: 10.1038/s41586
    Insert
    Cx34.7
    Use
    AAV
    Tags
    mEmerald
    Mutation
    E214K,E223K
    Promoter
    CaMKII
    Available since
    May 26, 2026
    Availability
    Academic Institutions and Nonprofits only
    255632-plasmid-map-sequence-id-511284

    Enlarge

  13. pAAV-CaMKII-Cx35-M1-FLAG-T2A-mCherry

    Plasmid
    #255639
    Purpose
    Encodes M. americana Cx35, codon optimized for mouse expression and c-terminally fused FLAG tag, T2A self-cleaving peptide and mCherry. Mutation of K221E. Expression driven by CaMKII promoter.
    Depositor
    Kafui Dzirasa
    Publication
    Ransey et al Nature. 2026 May 13. doi: 10.1038/s41586
    Insert
    Cx35
    Use
    AAV
    Tags
    FLAG-T2A-mCherry
    Mutation
    K221E
    Promoter
    CaMKII
    Available since
    May 26, 2026
    Availability
    Academic Institutions and Nonprofits only
    255639-plasmid-map-sequence-id-511283

    Enlarge

  14. pAAV-EF1⍺-DIO-Cx34.7-M1-mEmerald

    Plasmid
    #255634
    Purpose
    Encodes M. americana Cx34.7, codon optimized for mouse expression, with 8 amino acid linker and c-terminally fused mEmerald tag. Mutations of E214K and E223K. Expression driven by EF1a promoter.
    Depositor
    Kafui Dzirasa
    Publication
    Ransey et al Nature. 2026 May 13. doi: 10.1038/s41586
    Insert
    Cx34.7
    Use
    AAV
    Tags
    mEmerald
    Mutation
    E214K,E223K
    Promoter
    EF1a
    Available since
    May 26, 2026
    Availability
    Academic Institutions and Nonprofits only
    255634-plasmid-map-sequence-id-511281

    Enlarge

  15. pAAV-hSyn-Cx35-M1-FLAG-T2A-mCherry

    Plasmid
    #255640
    Purpose
    Encodes M. americana Cx35, codon optimized for mouse expression and c-terminally fused FLAG tag, T2A self-cleaving peptide and mCherry. Mutation of K221E. Expression driven by hSyn promoter.
    Depositor
    Kafui Dzirasa
    Publication
    Ransey et al Nature. 2026 May 13. doi: 10.1038/s41586
    Insert
    Cx35
    Use
    AAV
    Tags
    FLAG-T2A-mCherry
    Mutation
    K221E
    Promoter
    hSyn
    Available since
    May 26, 2026
    Availability
    Academic Institutions and Nonprofits only
    255640-plasmid-map-sequence-id-511280

    Enlarge

  16. pMXs-IRES-blast 3xFlag-GFP-SLC45A1(E774A, L778A, L779A)

    Plasmid
    #228523
    Purpose
    SLC45A1(E774A, L778A, L779A) codon-optimized orf with n-terminal 3xFlag and GFP in pMXs-IRES-Blast vector
    Depositor
    Monther Abu-Remaileh
    Publication
    Ghoochani et al Cell. 2026 Feb 5;189(3):765-782.e31. doi
    Insert
    SLC45A1 (SLC45A1 Human)
    Use
    Retroviral
    Tags
    Flag and GFP
    Mutation
    At position 774, glutamic acid (E) is replaced by…
    Available since
    April 21, 2026
    Availability
    Academic Institutions and Nonprofits only
    228523-plasmid-map-sequence-id-454855

    Enlarge

  19. EFS-SpdCas9-Dnmt3A/3L-V1

    Plasmid
    #222498
    Purpose
    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V1 (R887E) followed by P2A-mCherry
    Depositor
    Isaac Hilton
    Publication
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Insert
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V1 (R887E) engineered fusion
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; R887E in Dnmt3A
    Promoter
    EFS
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222498-plasmid-map-sequence-id-441135

    Enlarge

  20. EFS-SpdCas9-Dnmt3A/3L-V2

    Plasmid
    #222499
    Purpose
    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V2 (E814G) followed by P2A-mCherry
    Depositor
    Isaac Hilton
    Publication
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Insert
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V2 (E814G) engineered fusion
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E814G in Dnmt3A
    Promoter
    EFS
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222499-plasmid-map-sequence-id-441136

    Enlarge

Showing: 3481 - 3500 of 4853 results