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AiP14937 - pAAV-AiE1367m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4937)

Plasmid

#224053

Purpose

AiE1367m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L2/3_IT neurons (note HPF dentate gyrus)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

SYFP2-P2A-3XFLAG-10aa-H2B

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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AiP12547 - pAAV-hsA2-AiE0140h-minRho-mTFP1-WPRE3-BGHpA (Alias: CN2547)

Plasmid

#224061

Purpose

AiE0140h is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Pvalb+ neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

mTFP1

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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AiP1923 - pAAV-AiE2455m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214582

Purpose

AiE2455m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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Rtn3S-GFP

Plasmid

#169035

Purpose

Express human Rtn3S in mammalian cells

Depositor

Gia Voeltz

Article

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

Rtn3S (RTN3 Human)

Tags

acGFP1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAB1677 REPAIR.t3

Plasmid

#176320

Purpose

CMV-HIVNES-GS-dCas13bt3- (GGS)2-huADAR2dd(E488Q) (REPAIR.t3 expression)

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

Inserts

human codon optimized Cas13bt3 (catalytically inactivated)
huADAR2dd(E488Q) (ADARB1 Human)

Use

CRISPR

Tags

HIV NES

Expression

Mammalian

Mutation

E488Q, only the deaminase domain (aa 276-702 are …

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pECE HA AMPKa1 AS

Plasmid

#69831

Purpose

Expresses Expresses AMPK alpha 1 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)

Tags

HA

Expression

Mammalian

Promoter

SV40 early promoter

Available Since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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hMLKL-Venus-Plasma

Plasmid

#106081

Purpose

expression in mammalian cells

Depositor

Douglas Green

Article

Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

Insert

hMLKL-Venus-Plasma (MLKL Human)

Use

Retroviral; Tet-on retrovirus

Tags

plasma membrane-targetting domain of H-Ras (aa 1-…

Expression

Mammalian

Promoter

dox-inducible

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pIRESh-GFPII-S-Dlk1

Plasmid

#108931

Purpose

GFP-tagged vector expressing secreted isoform of Dlk1

Depositor

Anne Ferguson-Smith

Article

Ferron et al Nature. 2011 Jul 20;475(7356):381-5. doi

Insert

Dlk1 (Dlk1 Mouse)

Tags

GFPII

Expression

Mammalian

Mutation

contains sequences from full length Dlk1A up to t…

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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3xFLAG-CUL3-pCMV7.1

Plasmid

#155021

Purpose

N-terminal 3xFLAG-tagged CUL3 in a pCMV7.1 vector

Depositor

Andre Catic

Article

Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

Insert

Cullin 3 (CUL3 Human)

Tags

3xFLAG

Expression

Bacterial

Promoter

pCMV7.1

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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CMV ArcLightCo (Q239)-T2A-nls-mCherry

Plasmid

#85806

Purpose

Genetically encoded voltage sensor ArcLight- codon optimized

Depositor

Vincent Pieribone

Article

Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

Insert

ArcLightCo-Q239

Tags

mCherry

Expression

Mammalian

Mutation

Ci-VSP contains R217Q mutation; super ecliptic pH…

Promoter

CMV

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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p5M-hPR-B

Plasmid

#89114

Purpose

mammalian expression of full-length human progesterone receptor-B in pCMV5 with 5 restriction sites deleted in polylinker

Depositor

Elizabeth Wilson

Article

Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24

Insert

human progesterone receptor-B (PGR Human)

Expression

Mammalian

Mutation

5 restriction sites deleted in polylinker

Promoter

CMV

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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1099-N-cadherin-AAA-YFP

Plasmid

#32237

Depositor

Kathleen Green

Article

Chen et al J Cell Biol. 2003 Nov 10;163(3):547-57.

Insert

N-cadherin (Cdh2 Mouse)

Tags

EYFP

Expression

Mammalian

Mutation

Glu–Glu–Asp (aa 780–782) ---> Ala–Ala–Ala

Promoter

CMV

Available Since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only
T7-RelA(K221R)

Plasmid

#23248

Depositor

Warner Greene

Article

Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

Insert

NFkB RelA (RELA Human)

Tags

T7 gp10

Expression

Mammalian

Mutation

K221R

Available Since

March 15, 2010

Availability

Academic Institutions and Nonprofits only
pDN-D2irC6kwh

Plasmid

#44725

Depositor

Gabor Balazsi

Article

Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1

Inserts

pCMV-D2i promoter
mCherry

Use

Synthetic Biology; Expression regulator/reporter

Tags

Rabbit β-globin intron II and WPRE

Expression

Mammalian

Mutation

Initiator motif (Inr) displaced relative to pCMV-…

Promoter

pCMV-D2i

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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AiP12674 - pAAV-AiE0086h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2674)

Plasmid

#230124

Purpose

AiE0086h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Boaz Levi

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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AiP15256 - pAAV-AiE0664m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN5256)

Plasmid

#229931

Purpose

AiE0664m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific brain cell types

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Available Since

Sept. 12, 2025

Availability

Academic Institutions and Nonprofits only

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AiP14703 - pAAV-AiE2135m-minBG-iCre(R297T)-BGHpA (Alias: CN4703)

Plasmid

#229925

Purpose

AiE2135m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific brain cell types

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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AiP11580 - pAAV-hsA2-AiE0086m-minRho-SYFP2-WPRE3-BGHpA (Alias: CN1580)

Plasmid

#230099

Purpose

AiE0086m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Boaz Levi

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minRho

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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AiP1747 - pAAV-AiE2430m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#220665

Purpose

AiE2430m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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AiP15291 - pAAV-AiE0710m-minBG-iCre(R297T)-BGHpA (Alias: CN5291)

Plasmid

#230510

Purpose

AiE0710m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Promoter

minBG

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge