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PHAGE-P CMVt N-HA hE6-AP I (AA 1-64)

Plasmid

#119310

Purpose

lentiviral vector for expression of HA-Tagged E6-AP I AA 1-64 in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP I (UBE3A Human)

Use

Lentiviral

Tags

HA

Mutation

Only AA 1-64

Promoter

CMV

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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p8180 pHAGE N-V5 PTPN14 ΔPPXY3/4

Plasmid

#221647

Purpose

Constitutive ectopic expression of human PTPN14 ΔPPXY3/4 mutant

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

PTPN14 ΔPPXY3/4 (PTPN14 Human)

Use

Lentiviral

Tags

V5

Mutation

deletion of the third and fourth PPXY motifs as d…

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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p8291 MSCV-Neo C-HA 18E7 ΔDLLC

Plasmid

#184523

Purpose

Expression of HPV18 E7 ΔDLLC

Depositor

Elizabeth White

Article

Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

Insert

HPV18 E7 ΔDLLC

Use

Retroviral

Tags

HA

Mutation

deletion of DLLC

Promoter

MSCV LTR

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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p8195 MSCV-P C-FlagHA 6E7 R77S

Plasmid

#163309

Purpose

Expresses HPV6 E7 R77S

Depositor

Elizabeth White

Article

Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

Insert

HPV6 E7 R77S

Use

Retroviral

Tags

FlagHA

Mutation

R77S

Promoter

MSCV LTR

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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p8194 MSCV-P C-FlagHA 31E7 R77S

Plasmid

#163308

Purpose

Expresses HPV31 E7 R77S

Depositor

Elizabeth White

Article

Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

Insert

HPV31 E7 R77S

Use

Retroviral

Tags

FlagHA

Mutation

R77S

Promoter

MSCV LTR

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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p8193 MSCV-P C-FlagHA 18E7 R84S

Plasmid

#163307

Purpose

Expresses HPV18 E7 R84S

Depositor

Elizabeth White

Article

Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

Insert

HPV18 E7 R84S

Use

Retroviral

Tags

FlagHA

Mutation

R84S

Promoter

MSCV LTR

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pNCMV MmuPV1 E6 R130A

Plasmid

#87654

Purpose

Flag/HA tagged MmuPV1 E6 R130A

Depositor

Karl Munger

Article

Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

Insert

MmuPV1 E6

Tags

Flag and HA

Expression

Mammalian

Mutation

R130A

Promoter

CMV

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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NanoBRET Assay Donor Vector, E6AP

Plasmid

#237134

Purpose

Express E6AP-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

E6AP (UBE3A Human)

Tags

NanoLuc (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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p8224 pLIX-PTPN14 ΔPPXY1/2

Plasmid

#221644

Purpose

Doxycycline-inducible overexpression of human PTPN14 ΔPPXY1/2 mutant

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

PTPN14 ΔPPXY1/2 (PTPN14 Human)

Use

Lentiviral

Tags

HA

Mutation

deletion of the first and second (tandem) PPXY mo…

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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p8552 pLIX-PTPN14 ΔPPXY3/4

Plasmid

#221645

Purpose

Doxycycline-inducible overexpression of human PTPN14 ΔPPXY3/4 mutant

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

PTPN14 ΔPPXY3/4 (PTPN14 Human)

Use

Lentiviral

Tags

HA

Mutation

deletion of the third and fourth PPXY motifs as d…

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
p8215 pHAGE N-V5 PTPN14 ΔPPXY1/2

Plasmid

#221646

Purpose

Constitutive ectopic expression of human PTPN14 ΔPPXY1/2 mutant

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

PTPN14 ΔPPXY1/2 (PTPN14 Human)

Use

Lentiviral

Tags

V5

Mutation

deletion of the first and second (tandem) PPXY mo…

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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p8391 LentiCRISPRv2 Neo sgPTPN14-1

Plasmid

#221651

Purpose

Expression of sgRNA targeting the locus of human PTPN14

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

sgPTPN14-1 (PTPN14 Human)

Use

CRISPR and Lentiviral

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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p8392 LentiCRISPRv2 Neo sgPTPN14-3

Plasmid

#221652

Purpose

Expression of sgRNA targeting the locus of human PTPN14

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

sgPTPN14-3 (PTPN14 Human)

Use

CRISPR and Lentiviral

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-optimized E6AP F727D

Plasmid

#165101

Purpose

ecoli optimized expression of a GST fusion of Human E6AP F727D

Depositor

Arthur Haas

Article

Ronchi et al J Biol Chem. 2014 Jan 10;289(2):1033-48.

Insert

E6AP F727D (UBE3A Human)

Tags

GST from plasmid.

Expression

Bacterial

Mutation

codon optimized for E. coli expression

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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p8220 MSCV-Neo C-HA 18E7 R84S

Plasmid

#163312

Purpose

Expresses HPV18 E7 R84S

Depositor

Elizabeth White

Article

Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

Insert

HPV18 E7 R84S

Use

Retroviral

Tags

HA

Mutation

R84S

Promoter

MSCV LTR

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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PHAGE-P CMVt N-HA hE6-AP I (G20V)

Plasmid

#119308

Purpose

lentiviral vector for expression of HA-Tagged E6-AP I (G20V) in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP I (G20V) (UBE3A Human)

Use

Lentiviral

Tags

HA

Mutation

G20V

Promoter

CMV

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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PHAGE-P CMVt N-HA hE6-AP I (C21Y)

Plasmid

#119304

Purpose

lentiviral vector for expression of HA-Tagged E6-AP I (C21Y) in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP I (C21Y) (UBE3A Human)

Use

Lentiviral

Tags

HA

Mutation

C21Y

Promoter

CMV

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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PHAGE-P CMVt N-HA hE6-AP I (AA 55-376)

Plasmid

#119313

Purpose

lentiviral vector for expression of HA-Tagged E6-AP I AA 55-376 in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP I (UBE3A Human)

Use

Lentiviral

Tags

HA

Mutation

Only AA 55-376

Promoter

CMV

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PHAGE-P CMVt N-HA hE6-AP I (R39H)

Plasmid

#119309

Purpose

lentiviral vector for expression of HA-Tagged E6-AP I (R39H) in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP I (R39H) (UBE3A Human)

Use

Lentiviral

Tags

HA

Mutation

G20V

Promoter

CMV

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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PHAGE-P CMVt N-HA hE6-AP I (AA 55-852)

Plasmid

#119314

Purpose

lentiviral vector for expression of HA-Tagged E6-AP I AA 55-852 in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP I (UBE3A Human)

Use

Lentiviral

Tags

HA

Mutation

Only AA 55-852

Promoter

CMV

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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