We narrowed to 9,514 results for: try

1. pEPQD1CB0774

   Plasmid

   #177064

   Purpose

   MoClo Level 1, position 1, transcriptional unit for transient expression of tryptophan decarboxylase (CrTDC) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   tryptophan decarboxylase (CrTDC) from Catharanthus roseus
   

   Use

   Synthetic Biology

   Available Since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEPQD1CB0773

   Plasmid

   #177063

   Purpose

   MoClo Level 1, position 1, transcriptional unit for transient expression of tryptophan decarboxylase (CrTDC) from Catharanthus roseus driven by 35S promoter

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   tryptophan decarboxylase (CrTDC) from Catharanthus roseus
   

   Use

   Synthetic Biology

   Available Since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDule2-pCNF

   Plasmid

   #85495

   Purpose

   Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.

   Depositor

   Ryan Mehl

   Article

   Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62

   Insert

   para-cyanophenylalanine Mj synthetase
   

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32L L65V F108W Q109M D158G I159P

   Promoter

   lpp (constitutive)

   Available Since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. piggyBAC-H2B FR-MQV

   Plasmid

   #194079

   Purpose

   Expresses nuclear-localized FR-MQV in mammalian cells

   Depositor

   Ralph Jimenez

   Article

   Mukherjee et al Biochemistry. 2020 Oct 6;59(39):3669-368

   Insert

   FR-MQV
   

   Tags

   Histone H2B

   Expression

   Mammalian

   Mutation

   Methionine 42 to Glutamine, Cystiene 159 to Valin…

   Promoter

   CMV7

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDule-pCNF

   Plasmid

   #85494

   Purpose

   Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.

   Depositor

   Ryan Mehl

   Article

   Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62

   Insert

   para-cyanophenylalanine Mj synthetase
   

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32L L65V F108W Q109M D158G I159P

   Promoter

   lpp (constitutive)

   Available Since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-UBC-hAAT-IRES-ZsGreen-W

   Plasmid

   #126702

   Purpose

   lentiviral bicistronic exprression of both human alpha-1 anitrypsin and ZsGreen

   Depositor

   Darrell Kotton

   Article

   Wilson et al Am J Respir Cell Mol Biol. 2008 Aug . 39

   Insert

   Alpha-1 antitrypsin (hAAT aka SERPINA1) and ZsGreen; "AAT-IRES-ZSGreen" (SERPINA1 Human)
   

   Use

   Lentiviral

   Promoter

   UBC

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. tdTomato/pTREX-b

   Plasmid

   #68709

   Purpose

   Expresses tdTomato in pTREX-b vector which confers resistance to blasticidin. This vector is used for cloning a specific sgRNA by BamHI, to transfect Cas9-expressing Trypanosoma cruzi epimastigotes.

   Depositor

   Roberto Docampo

   Article

   Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

   Insert

   tdTomato
   

   Use

   Expression of tdtomato red fluorescence protein i…

   Available Since

   Oct. 8, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. H1x

   Plasmid

   #218111

   Purpose

   Encodes mus musculus histone 1 in Gateway entry vector

   Depositor

   Peter Hamilton

   Article

   Kim et al Transl Psychiatry. 2024 Jun 5;14(1):239.

   Insert

   Histone H1x (H1f10 Mouse)
   

   Use

   Gateway cloning entry vector

   Expression

   Bacterial

   Available Since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. KG#797

   Plasmid

   #110916

   Purpose

   Expresses the active zone - enriched protein Sentryn-GFP in a subset of cholinergic ventral nerve cord motor neurons

   Depositor

   Kenneth Miller

   Article

   Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:

   Inserts

   unc-129 promoter
   STRN-1 (Sentryn)-GFP
   unc-54 3' control region with 1 intron just upstream and 1 intron in control region
   

   Tags

   GFP (contains 3 artificial introns)

   Expression

   Bacterial

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. SpIMPDH

    Plasmid

    #206980

    Purpose

    Streptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.

    Depositor

    Vern Schramm

    Article

    Glockzin et al Biochemistry. 2022 Oct 4;61(19):2088-210

    Insert

    SpIMPDH (guaB Streptococcus pyogenes)
    

    Tags

    His tag (with TEV cleavage site)

    Expression

    Bacterial

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET26b(+)-ATI_QconCAT_1

    Plasmid

    #163957

    Purpose

    Exression of ATI QconCAT protein in E. coli expression strains (BL21). Encodes a concatemer of tryptic peptides from wheat amylase/trypsin inhibitors. Used as standard for LC-MS-based quantification.

    Depositor

    Stefan Tenzer

    Article

    Sielaff et al J Proteome Res. 2021 Jan 28. doi: 10.102

    Insert

    ATI QconCAT
    

    Tags

    polyhistidine tag

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUB39

    Plasmid

    #26300

    Depositor

    George Cross

    Insert

    VSG117 (Lister 427-4) cDNA
    

    Use

    Trypanosome inducible expression vector

    Available Since

    Sept. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pME-QF2

    Plasmid

    #83307

    Purpose

    codes for QF2 (truncated QF) in a gateway middle entry backbone.

    Depositor

    Marnie Halpern

    Insert

    QF
    

    Available Since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAIP4_2H7W

    Plasmid

    #234220

    Purpose

    Heterologous protein expression of chagasin in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    2H7W (Tc00.1047053506801.80 Trypanosoma cruzi)
    

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. PP-ASL

    Plasmid

    #40749

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    Trypanosoma Brucei Adenylosuccinate Lyase
    

    Tags

    6xHis TEV

    Expression

    Bacterial

    Available Since

    Oct. 18, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

16. Tol2-zTRAP

    Plasmid

    #42236

    Depositor

    Stephen L Johnson

    Article

    Tryon et al Genesis. 2012 Dec 19. doi: 10.1002/dvg.2

    Insert

    EGFP-Rpl10a (rpl10a Zebrafish)
    

    Tags

    EGFP

    Available Since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLEW100cre-EP1-6G

    Plasmid

    #33344

    Depositor

    George Cross

    Article

    Scahill et al Mol Biochem Parasitol. 2008 Jan;157(1):7

    Insert

    cre recombinase
    

    Use

    Trypanosoma brucei expression vector

    Available Since

    Dec. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJK383

    Plasmid

    #72474

    Purpose

    Produces Thermoplasma acidophilum citrate synthase with Asp317>Gly mutant (TpCS-D317G)

    Depositor

    T. J. Kappock

    Insert

    citrate synthase
    

    Expression

    Bacterial

    Mutation

    changes tryptophan-17 to phenylalanine, tryptopha…

    Promoter

    recA

    Available Since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. Tol2-ftyrp>TRAP

    Plasmid

    #42299

    Depositor

    Stephen L Johnson

    Article

    Tryon et al Genesis. 2012 Dec 19. doi: 10.1002/dvg.2

    Insert

    EGFP-Rpl10a (rpl10a Zebrafish)
    

    Tags

    EGFP

    Promoter

    Takifugu rubripes tyrosine related protein 1 (tyr…

    Available Since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT7-stl

    Plasmid

    #51721

    Purpose

    conditional gene knockdowns in Trypanosoma brucei

    Depositor

    Arthur Gunzl

    Article

    Brandenburg et al EMBO J. 2007 Nov 28;26(23):4856-66. Epub

    Type

    Empty backbone

    Use

    Conditional gene knockdown in t. brucei

    Promoter

    T7 (2X)

    Available Since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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