We narrowed to 15,748 results for: pET

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Arabidopsis thaliana Atm3 d80 pET21a+

Plasmid

#173045

Purpose

Expresses AtAtm3 in E.coli

Depositor

Douglas Rees

Article

Fan et al Elife. 2022 Mar 25;11. pii: 76140. doi:

Insert

Arabidopsis thaliana Atm3 (ABCB25 Mustard Weed)

Tags

6xHis tag

Expression

Bacterial

Mutation

with N-terminal 80 amino acids deletion

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pET51b(+)-NADPH-LUCID-1 (C50 5.1 nM)

Plasmid

#117908

Purpose

Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 5.1 nM

Depositor

Kai Johnsson

Article

Yu et al Science. 2018 Sep 14;361(6407):1122-1126

Insert

SNAP_Pro30_[cpNLuc23_24]eDHFR

Tags

His-tag and Strep-tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pET23A-Fucosidase-E1_10125-Ruminococcus_gnavus-His

Plasmid

#207665

Purpose

Expresses fucosidase E1_10125 from Ruminococcus gnavus in BL21 cells, purified using His-tag

Depositor

Robert de Vries

Article

Spruit et al J Virol. 2024 Apr 16;98(4):e0194123. doi

Insert

Fucosidase E1_10125

Tags

His-tag

Expression

Bacterial

Mutation

amino acids 34-584 were used

Promoter

T7 promoter

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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FL MALT1 Catalytic Mutant in pET29b-Strep

Plasmid

#48969

Purpose

Expresses Full length catalytic mutant MALT1 in e.coli

Depositor

Guy Salvesen

Article

Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

Insert

MALT1 isoform A (MALT1 Human)

Tags

His and Strep

Expression

Bacterial

Mutation

Catalytic Mutant C464A

Promoter

T7

Available Since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only
pET28a-ybbr-HIS-ddFLN4-Fgß

Plasmid

#101719

Purpose

wildtype Fgß peptide with ddFLN4 fingerprint domain with N-terminal ybbr tag for covalent immobilization - resulting in weak forces

Depositor

Hermann Gaub

Article

Milles et al Science. 2018 Mar 30;359(6383):1527-1533

Insert

ddFLN4 fingerprint, Fgß peptide (FGB Human, Dictyostelium discoideum)

Tags

HIS and ybbr

Expression

Bacterial

Mutation

C18S in ddFLN4

Promoter

T7

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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HOXB4 (human) HA-TAT-tag pET

Plasmid

#8526

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB4 (HOXB4 Human)

Tags

HA

Expression

Bacterial

Mutation

replace T7 and HIS tags of pET with an HA tag;

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
BPV00605 pET21GG2-His(6)-BECN(1-265)

Plasmid

#136686

Purpose

Expresses the Beclin-1 IDR+CC domains (a.a. 1-265) with a N-terminal hexa-Histidine tag

Depositor

Colin Garvie

Article

Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66

Insert

Beclin-1 IDR + CC (BECN1 Human)

Tags

Hexa-Histadine

Expression

Bacterial

Mutation

DNA optimized for E. coli expression

Promoter

T7

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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PET-B-SpCas9-HF1-NLS-6xHis

Plasmid

#207385

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-SpCas9-HF1

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

N497A, R661A, Q695A, Q926A, amino acids 1005-1013…

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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HOXB7 (human) HIS-tag pET

Plasmid

#8538

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB7 (HOXB7 Human)

Tags

His and T7

Expression

Bacterial

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
human KANK1 Ankyrin Repeats pET151

Plasmid

#164053

Purpose

Expresses human KANK1 Ank domain in bacterial cells

Depositor

Ben Goult

Insert

Kidney Ankyrin Repeat-Containing Protein 1 (KANK1 Human)

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pET-3a-His-MBP-TEV-VC_Cas1_WT

Plasmid

#120364

Purpose

Expresses wildtype V-C Cas1

Depositor

Jennifer Doudna

Article

Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

Insert

VC_Cas1

Use

CRISPR

Expression

Bacterial

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET-3a-His-MBP-TEV-VD_Cas1_WT

Plasmid

#120365

Purpose

Expresses wildtype V-D Cas1

Depositor

Jennifer Doudna

Article

Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

Insert

VD_Cas1

Use

CRISPR

Expression

Bacterial

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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HOXB1 (human) HIS-tag pET

Plasmid

#8520

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXB1 (HOXB1 Human)

Tags

His and T7

Expression

Bacterial

Mutation

PCR from cDNA clone

Available Since

July 6, 2006

Availability

Academic Institutions and Nonprofits only
pET-45b-NFYC-IDR-mEGFP

Plasmid

#185015

Purpose

Bacterial Expression of 6xHis NFYC IDR Sirius-GFP Fusion Protein

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

NFYC IDR (Nfyc Mouse)

Expression

Bacterial

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pET29-hMIGA2(306-593)-6xhis

Plasmid

#192837

Purpose

Express codon-optimized human MIGA2 fragment 306-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteria

Depositor

Karin Reinisch

Article

Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

Insert

Codon-optimized human Mitoguardin 2 (MIGA2 Human)

Tags

6xhis

Expression

Bacterial

Promoter

T7

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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HIV-1 integrase W235F in pET-15b

Plasmid

#61681

Purpose

HIV-1 integrase W235F in pET-15b

Depositor

Robert Craigie

Article

Li et al EMBO J. 2006 Mar 22;25(6):1295-304. Epub

Insert

HIV-1 integrase W235F

Tags

His

Mutation

W235F

Promoter

T7

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only
HOXB3 (human) HIS-tag pET

Plasmid

#8524

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXB3 (HOXB3 Human)

Tags

His and T7

Expression

Bacterial

Mutation

Contains 5 additional amino acids before ATG from…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
HOXC8 (murine) HIS-tag pET

Plasmid

#8567

Depositor

Corey Largman

Insert

HOXC8 (Hoxc8 Mouse)

Tags

His and T7

Expression

Bacterial

Mutation

makes protein by Western

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pET28a SUMO Nup53xl 162-267

Plasmid

#86002

Purpose

bacterial expression of his6 and SUMO tagged RRM motif of Xenopus laevis Nup53

Depositor

Wolfram Antonin

Type

Empty backbone

Tags

SUMO

Expression

Bacterial

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pET-28a-His6-SUMO-VAMP8

Plasmid

#135553

Purpose

Express mouse VAMP8 in E. coli BL21, purified via HisPur Ni-NTA Resin

Depositor

Jingshi Shen

Article

Yu et al Methods Mol Biol. 2019;1860:237-249. doi

Insert

VAMP8

Tags

6xHis tag, thrombin recognition and cleavage site…

Expression

Bacterial

Mutation

No ATG start codon

Promoter

T7 promoter

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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