1. pminiTol2

   Plasmid

   #31829

   Depositor

   Stephen Ekker

   Article

   Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. EcNGsC-DelFSH(307-309)-pKK223

   Plasmid

   #131377

   Purpose

   Low level expression of E.coli G.stearothermophillus MutY chimera protein with deletion of residues FSH 307-309 in Gs MutY.

   Depositor

   Martin Horvath

   Article

   Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

   Insert

   EcNGsC MutY chimera DelFSH(307-309)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Fusion of two MutY genes. Residues 1-225 are from…

   Promoter

   tac

   Available since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. EcNGsC-S308P-H309D-pKK223

   Plasmid

   #131378

   Purpose

   Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid changes S308P and H309D in Gs MutY.

   Depositor

   Martin Horvath

   Article

   Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

   Insert

   EcNGsC MutY chimera S308P H309D
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Fusion of two MutY genes. Residues 1-225 are from…

   Promoter

   tac

   Available since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. EcNGsC-F307A-S308A-pKK223

   Plasmid

   #131379

   Purpose

   Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid changes F307A and S308A in Gs MutY.

   Depositor

   Martin Horvath

   Article

   Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

   Insert

   EcNGsC MutY chimera F307A S308A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Fusion of two MutY genes. Residues 1-225 are fro…

   Promoter

   tac

   Available since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. 4RCI

   Plasmid

   #64653

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   YTHDF1:JMC02A-E04:C231829 (YTHDF1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   See comments

   Promoter

   T7

   Available since

   April 29, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits