161,838 results

1. Tet-pLKO-puro

   Plasmid

   #21915

   Purpose

   3rd generation lentiviral plasmid for inducible expression of shRNA; puromycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.

   Depositor

   Dmitri Wiederschain

   Article

   Wiederschain et al Cell Cycle. 2009 Feb 1. 8(3):498-504.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-PE7

   Plasmid

   #214812

   Purpose

   Mammalian expression of SpCas9 PE7 prime editor

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7
   

   Use

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB533-42B3mutAC2-sfGFP

   Plasmid

   #186777

   Purpose

   RNAP2 Ser2ph-mintbody [42B3(R78K/A80T/M95V)-scFv] expression vector for mammalian cells

   Depositor

   Hiroshi Kimura

   Article

   Uchino et al J Cell Biol. 2022 Feb 7;221(2). pii: 212

   Insert

   42B3mutAC2-scFv
   

   Use

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV x EF1a_EKAREN5-ires-puro

   Plasmid

   #167821

   Purpose

   Optimized EKAREV FRET biosensor sensor for ERK

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN5
   

   Use

   Lentiviral

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W

   Promoter

   EF1a

   Available since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. EKAREN5-gl

   Plasmid

   #225958

   Purpose

   The YPet and mTurquoise sequence cassettes in EKAREN5(Addgene 167821) were replaced with a synonymous codon variant of YPet and mTurquoise-gl and subcloned into pCSII lentiviral backbone.

   Depositor

   Toru Hiratsuka

   Article

   Weisheng et al Cell Struct Funct. 2024 Dec 18. doi: 10.

   Insert

   EKAREN5-gl
   

   Use

   Lentiviral

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1-alpha promoter

   Available since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLJC5-3XHA-EGFP-PEX26

   Plasmid

   #139054

   Purpose

   HA-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.

   Depositor

   David Sabatini

   Article

   Ray et al iScience. 2020 May 22;23(5):101109. doi:

   Insert

   3XHA-EGFP-PEX26
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   UBC

   Available since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-luciferase-P2A-Neo

   Plasmid

   #105621

   Purpose

   3rd generation lentiviral plasmid for expression of luciferase with Neo resistance

   Depositor

   Christopher Vakoc

   Article

   Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

   Insert

   Luciferase
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-p300

   Plasmid

   #23252

   Depositor

   Warner Greene

   Article

   Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

   Insert

   p300 (EP300 Human)
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDH-3xFLAG-TERT

   Plasmid

   #51631

   Purpose

   Mammalian expression of 3xFLAG tagged TERT, N-terminal.

   Depositor

   Steven Artandi

   Article

   Zhong et al Cell. 2012 Aug 3;150(3):481-94. doi: 10.

   Insert

   TERT (TERT Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. FKBP-V5-AP-nes_pLX304

    Plasmid

    #120912

    Purpose

    Lentiviral vector expressing FKBP-V5-AP-NES in mammalian cytosol

    Depositor

    Alice Ting

    Article

    Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

    Insert

    FKBP-V5-AP-NES
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. HA-Halotag-FRB-EX-nes_pLX304

    Plasmid

    #120913

    Purpose

    Lentiviral vector expressing HA-Halotag-FRB-EX-NES in mammalian cytosol

    Depositor

    Alice Ting

    Article

    Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

    Insert

    HA-Halotag-FRB-EX-NES
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. myc-mTOR

    Plasmid

    #1861

    Depositor

    David Sabatini

    Article

    Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

    Insert

    mTOR (Mtor Rat)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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13. U6-BbsI-GGsite-mCherry-Puro

    Plasmid

    #221232

    Purpose

    Cloning vector for sgRNA with mCherry and Puro

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. CF-Ins54p

    Plasmid

    #20155

    Depositor

    Tobias Meyer

    Article

    Suh et al Science. 2006 Dec 1. 314(5804):1454-7.

    Insert

    CFP-FKBP-Inp54p (INP54 Budding Yeast)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Stop codon at position 331.

    Promoter

    Available since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21 gamma-tubulin

    Plasmid

    #101825

    Purpose

    production of gamma-tubulin in bacteria

    Depositor

    Ana Carrera, Maria Alvarado-Kristensson

    Article

    Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

    Insert

    gamma-tubulin 1 (TUBG1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Whole protein

    Promoter

    T7

    Available since

    Oct. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. CF-Inp(D281A)

    Plasmid

    #20156

    Depositor

    Tobias Meyer

    Article

    Suh et al Science. 2006 Dec 1. 314(5804):1454-7.

    Insert

    CFP-FKBP-Inp54p (D281A) (INP54 Budding Yeast)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Stop codon at position 331. D281A mutation.

    Promoter

    Available since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. CSII-PPM1H-mApple

    Plasmid

    #198473

    Purpose

    Mammalian expression of PPM1H-mApple

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    PPM1H-mApple (PPM1H Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-EF1a-IRES-Puro

    Plasmid

    #85132

    Purpose

    lentiviral backbone

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV(CAT)T7-SB100

    Plasmid

    #34879

    Depositor

    Zsuzsanna Izsvak

    Article

    Mates et al Nat Genet. 2009 Jun;41(6):753-61. Epub 2

    Insert

    SB100X transposase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-19b_MMLV-RT

    Plasmid

    #153312

    Purpose

    Bacterial expression of MMLV reverse transcriptase

    Depositor

    Drew Endy, Philippa Marrack

    Insert

    MMLV Reverse Transcriptase
    

    Use

    Tags

    10x His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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