163,637 results

1. pA-His6-Tn5

   Plasmid

   #171871

   Purpose

   Expresses protein A and Tn5 Transposase fusion protein in bacterial cells

   Depositor

   Kaiwei Liang

   Article

   Wang et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabe

   Insert

   Protein A and hyperactive Tn5 transposase
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   Available Since

   July 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBB541

   Plasmid

   #27394

   Depositor

   Bernd Bukau

   Article

   de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

   Insert

   groESL (groL, groS E. coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCXWB-EBNA1

   Plasmid

   #37624

   Purpose

   Non-replicating episomal expression of EBNA1, which enhances transfection efficiency and expression from episomal plasmids

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

   Insert

   EBNA-1 (EBNA-1 human herpesvirus 4)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Dec. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-CMV-LoxP-DsRed-LoxP-eGFP

   Plasmid

   #65726

   Purpose

   Functions as a genetic Cre reporter that switches from DsRed expression to eGFP expression upon presence of Cre recombinase.

   Depositor

   Jacco van Rheenen

   Article

   Zomer et al Cell. 2015 May 21;161(5):1046-57. doi: 1

   Insert

   LoxP-DsRed-LoxP-eGFP
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-PE6d

   Plasmid

   #207854

   Purpose

   Mammalian expression of PE6d prime editor

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   PE6d
   

   Use

   Tags

   SV40 bpNLS and SV40 bpNLS, c-Myc NLS

   Expression

   Mammalian

   Mutation

   M-MLVRTDRNAseHrt(T128N, V223Y, D200C)

   Promoter

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. tFucci(CA)5

   Plasmid

   #153521

   Purpose

   New color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(CA)5.

   Depositor

   Atsushi Miyawaki

   Article

   Ando et al Cell Struct Funct. 2023 Jun 30. doi: 10.

   Insert

   tFucci(CA)5.
   

   Use

   Tags

   AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPR-HOT_tdTomato

   Plasmid

   #138567

   Purpose

   Universal Cleavable Donor Plasmid for tagging with tdTomato using NHEJ

   Depositor

   Hans Clevers

   Article

   Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

   Insert

   tdTomato
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. EZ-Tet-pLKO-Puro

   Plasmid

   #85966

   Purpose

   Tet/Dox inducible shRNA lentivirus with puromycin selection

   Depositor

   Cindy Miranti

   Article

   Frank et al BMC Biotechnol. 2017 Feb 28;17(1):24. do

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   H1 / TetO

   Available Since

   Feb. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. [#GS7-LV] DsRed GFP TEVp Zeo Switch - Recipient

   Plasmid

   #233507

   Purpose

   Lentiviral construct for the MitoTRACER genetic reporter to be expressed in the recipient cells

   Depositor

   Simon Grelet

   Article

   Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

   Insert

   DsRed loxP eGFP
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. Gal4-VP16

    Plasmid

    #71728

    Purpose

    plasmid expressing fusion construct of Gal4 DBD and VP16 AD

    Depositor

    Lea Sistonen

    Article

    Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

    Insert

    Gal4 DBD(1-147) (GAL4 Budding Yeast)
    

    Use

    Tags

    VP16 AD (413-490)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. H6GB1tevP53SS(1-393)

    Plasmid

    #200311

    Purpose

    Bacterial expression of human superstable p53 as His6GB1tev fusion

    Depositor

    Peter Wright

    Article

    Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His6-GB1-tev

    Expression

    Bacterial

    Mutation

    M133L, V203A, N239Y, N268D

    Promoter

    T7

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. dCas9-KRAB-MeCP2

    Plasmid

    #110821

    Purpose

    Improved dCas9 repressor-dCas9-KRAB-MeCP2

    Depositor

    Alejandro Chavez, George Church

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CMV

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. kiCAP-AAV-MyoAAV2A

    Plasmid

    #224440

    Purpose

    Rep/Cap plasmid for the production of MyoAAV 2A, a muscle-tropic AAV capsid in mice.

    Depositor

    Pardis Sabeti

    Article

    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

    Insert

    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    

    Use

    AAV

    Tags

    Expression

    Mutation

    RGDQTTL insert between amino acids 588 and 589 of…

    Promoter

    p41

    Available Since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. lentiCas9-EGFP

    Plasmid

    #63592

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9 protein and EGFP from EFS promoter. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang, Phil Sharp

    Article

    Chen et al Cell. 2015 Mar 12;160(6):1246-60. doi: 1

    Inserts

    Cas9
    EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG and P2A

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available Since

    April 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR-Traditional CAR (anti-CD19)

    Plasmid

    #192115

    Purpose

    Lentiviral delivery of Traditional CAR(anti-CD19)

    Depositor

    Wilson Wong

    Article

    Li et al Cancer Cell. 2022 Aug 26. pii: S1535-610

    Insert

    pHR-pEF1a-Traditional CAR (anti-CD19scFV_CD28_41BB_CD3z)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTNS2

    Plasmid

    #64968

    Purpose

    Tn7 transposase expression

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Insert

    tnsABCD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pCRISPR-Cas3

    Plasmid

    #209427

    Purpose

    Plasmid for CASCADE-Cas3 based genome engineering of streptomycetes

    Depositor

    Tilmann Weber

    Article

    Whitford et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

    Insert

    codon optimized minimal type I-C CASCADE-Cas3 from Pseudomonas aeruginosa
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PtipA; crRNA under control of PermE*

    Available Since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLEX_307

    Plasmid

    #41392

    Purpose

    Constitutive lentiviral expression, SV40-puro; EF1a-gateway-V5 tag

    Depositor

    David Root

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Nov. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pU6-PspCas13b-gRNA-Actb1216

    Plasmid

    #155368

    Purpose

    PspCas13b guide RNA positioned 8bp 5' of Actb 1216 for targeted m6A RNA methylation

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    PspCas13b gRNA targeting Actb 1216
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPgiCas13b

    Plasmid

    #184833

    Purpose

    Inducible expression of PgiCas13b nuclease in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    Cas13b nuclease from Porphyromonas gingivalis
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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