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pSpCas9n(BB)-2A-Puro (PX462) V2.0 Citations (56)

Plasmid Article: Genome engineering using the CRISPR-Cas9 system.
Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24.
PubMed Journal

Articles Citing pSpCas9n(BB)-2A-Puro (PX462) V2.0

Articles
HPF1/C4orf27 Is a PARP-1-Interacting Protein that Regulates PARP-1 ADP-Ribosylation Activity. Gibbs-Seymour I, Fontana P, Rack JG, Ahel I. Mol Cell. 2016 May 5;62(3):432-42. doi: 10.1016/j.molcel.2016.03.008. Epub 2016 Apr 7. PubMed
IQGAP1 Binds to Yes-associated Protein (YAP) and Modulates Its Transcriptional Activity. Sayedyahossein S, Li Z, Hedman AC, Morgan CJ, Sacks DB. J Biol Chem. 2016 Sep 9;291(37):19261-73. doi: 10.1074/jbc.M116.732529. Epub 2016 Jul 20. PubMed
Comprehensive Protocols for CRISPR/Cas9-based Gene Editing in Human Pluripotent Stem Cells. Santos DP, Kiskinis E, Eggan K, Merkle FT. Curr Protoc Stem Cell Biol. 2016 Aug 17;38:5B.6.1-5B.6.60. doi: 10.1002/cpsc.15. PubMed
Generation of a TLE3 heterozygous knockout human embryonic stem cell line using CRISPR-Cas9. Bara AM, Messana A, Herring A, Hazelbaker DZ, Eggan K, Barrett LE. Stem Cell Res. 2016 Sep;17(2):441-443. doi: 10.1016/j.scr.2016.09.008. Epub 2016 Sep 15. PubMed
Long non-coding RNAs transcribed by ERV-9 LTR retrotransposon act in cis to modulate long-range LTR enhancer function. Hu T, Pi W, Zhu X, Yu M, Ha H, Shi H, Choi JH, Tuan D. Nucleic Acids Res. 2017 May 5;45(8):4479-4492. doi: 10.1093/nar/gkx055. PubMed
Hypermethylated LTR retrotransposon exhibits enhancer activity. Hu T, Zhu X, Pi W, Yu M, Shi H, Tuan D. Epigenetics. 2017 Mar 4;12(3):226-237. doi: 10.1080/15592294.2017.1289300. Epub 2017 Feb 6. PubMed
Lipid transport by TMEM24 at ER-plasma membrane contacts regulates pulsatile insulin secretion. Lees JA, Messa M, Sun EW, Wheeler H, Torta F, Wenk MR, De Camilli P, Reinisch KM. Science. 2017 Feb 17;355(6326). pii: eaah6171. doi: 10.1126/science.aah6171. PubMed
Allele-specific ablation rescues electrophysiological abnormalities in a human iPS cell model of long-QT syndrome with a CALM2 mutation. Yamamoto Y, Makiyama T, Harita T, Sasaki K, Wuriyanghai Y, Hayano M, Nishiuchi S, Kohjitani H, Hirose S, Chen J, Yokoi F, Ishikawa T, Ohno S, Chonabayashi K, Motomura H, Yoshida Y, Horie M, Makita N, Kimura T. Hum Mol Genet. 2017 May 1;26(9):1670-1677. doi: 10.1093/hmg/ddx073. PubMed
Serine 392 phosphorylation modulates p53 mitochondrial translocation and transcription-independent apoptosis. Castrogiovanni C, Waterschoot B, De Backer O, Dumont P. Cell Death Differ. 2018 Jan;25(1):190-203. doi: 10.1038/cdd.2017.143. Epub 2017 Sep 22. PubMed
Parallel genome-wide screens identify synthetic viable interactions between the BLM helicase complex and Fanconi anemia. Moder M, Velimezi G, Owusu M, Mazouzi A, Wiedner M, Ferreira da Silva J, Robinson-Garcia L, Schischlik F, Slavkovsky R, Kralovics R, Schuster M, Bock C, Ideker T, Jackson SP, Menche J, Loizou JI. Nat Commun. 2017 Nov 1;8(1):1238. doi: 10.1038/s41467-017-01439-x. PubMed
Evaluation and validation of commercial antibodies for the detection of Shb. Vanli G, Cuesta-Marban A, Widmann C. PLoS One. 2017 Dec 1;12(12):e0188311. doi: 10.1371/journal.pone.0188311. eCollection 2017. PubMed
Versatile single-step-assembly CRISPR/Cas9 vectors for dual gRNA expression. Adikusuma F, Pfitzner C, Thomas PQ. PLoS One. 2017 Dec 6;12(12):e0187236. doi: 10.1371/journal.pone.0187236. eCollection 2017. PubMed

Associated Plasmids

Replication Stress Shapes a Protective Chromatin Environment across Fragile Genomic Regions. Kim J, Sturgill D, Sebastian R, Khurana S, Tran AD, Edwards GB, Kruswick A, Burkett S, Hosogane EK, Hannon WW, Weyemi U, Bonner WM, Luger K, Oberdoerffer P. Mol Cell. 2018 Jan 4;69(1):36-47.e7. doi: 10.1016/j.molcel.2017.11.021. Epub 2017 Dec 14. PubMed
p62 filaments capture and present ubiquitinated cargos for autophagy. Zaffagnini G, Savova A, Danieli A, Romanov J, Tremel S, Ebner M, Peterbauer T, Sztacho M, Trapannone R, Tarafder AK, Sachse C, Martens S. EMBO J. 2018 Mar 1;37(5). pii: embj.201798308. doi: 10.15252/embj.201798308. Epub 2018 Jan 17. PubMed

Associated Plasmids

Activation of the Anti-Aging and Cognition-Enhancing Gene Klotho by CRISPR-dCas9 Transcriptional Effector Complex. Chen CD, Zeldich E, Li Y, Yuste A, Abraham CR. J Mol Neurosci. 2018 Feb;64(2):175-184. doi: 10.1007/s12031-017-1011-0. Epub 2018 Jan 19. PubMed
Removal of prolyl oligopeptidase reduces alpha-synuclein toxicity in cells and in vivo. Svarcbahs R, Julku UH, Norrbacka S, Myohanen TT. Sci Rep. 2018 Jan 24;8(1):1552. doi: 10.1038/s41598-018-19823-y. PubMed
BRD4 interacts with NIPBL and BRD4 is mutated in a Cornelia de Lange-like syndrome. Olley G, Ansari M, Bengani H, Grimes GR, Rhodes J, von Kriegsheim A, Blatnik A, Stewart FJ, Wakeling E, Carroll N, Ross A, Park SM, Bickmore WA, Pradeepa MM, FitzPatrick DR. Nat Genet. 2018 Mar;50(3):329-332. doi: 10.1038/s41588-018-0042-y. Epub 2018 Jan 29. PubMed
A peptide tag-specific nanobody enables high-quality labeling for dSTORM imaging. Virant D, Traenkle B, Maier J, Kaiser PD, Bodenhofer M, Schmees C, Vojnovic I, Pisak-Lukats B, Endesfelder U, Rothbauer U. Nat Commun. 2018 Mar 2;9(1):930. doi: 10.1038/s41467-018-03191-2. PubMed

Associated Plasmids

STING-Dependent Interferon-lambda1 Induction in HT29 Cells, a Human Colorectal Cancer Cell Line, After Gamma-Radiation. Chen J, Markelc B, Kaeppler J, Ogundipe VML, Cao Y, McKenna WG, Muschel RJ. Int J Radiat Oncol Biol Phys. 2018 May 1;101(1):97-106. doi: 10.1016/j.ijrobp.2018.01.091. Epub 2018 Feb 3. PubMed
Coordinated Control of mRNA and rRNA Processing Controls Embryonic Stem Cell Pluripotency and Differentiation. Corsini NS, Peer AM, Moeseneder P, Roiuk M, Burkard TR, Theussl HC, Moll I, Knoblich JA. Cell Stem Cell. 2018 Apr 5;22(4):543-558.e12. doi: 10.1016/j.stem.2018.03.002. PubMed
IKK1/2 protect human cells from TNF-mediated RIPK1-dependent apoptosis in an NF-kappaB-independent manner. Slotta C, Storm J, Pfisterer N, Henkel E, Kleinwachter S, Pieper M, Ruiz-Perera LM, Greiner JFW, Kaltschmidt B, Kaltschmidt C. Biochim Biophys Acta Mol Cell Res. 2018 Aug;1865(8):1025-1033. doi: 10.1016/j.bbamcr.2018.04.003. Epub 2018 Apr 7. PubMed
Galectin-3 modulates the polarized surface delivery of beta1-integrin in epithelial cells. Honig E, Ringer K, Dewes J, von Mach T, Kamm N, Kreitzer G, Jacob R. J Cell Sci. 2018 Jun 11;131(11). pii: jcs.213199. doi: 10.1242/jcs.213199. PubMed
FRMD8 promotes inflammatory and growth factor signalling by stabilising the iRhom/ADAM17 sheddase complex. Kunzel U, Grieve AG, Meng Y, Sieber B, Cowley SA, Freeman M. Elife. 2018 Jun 13;7. pii: 35012. doi: 10.7554/eLife.35012. PubMed
Integrin beta1 is an essential factor in vasculogenic mimicry of human cancer cells. Kawahara R, Niwa Y, Simizu S. Cancer Sci. 2018 Aug;109(8):2490-2496. doi: 10.1111/cas.13693. Epub 2018 Jul 9. PubMed
A new genetic tool to improve immune-compromised mouse models: Derivation and CRISPR/Cas9-mediated targeting of NRG embryonic stem cell lines. Martin Gonzalez J, Baudet A, Abelechian S, Bonderup K, d'Altri T, Porse B, Brakebusch C, Juliusson G, Cammenga J. Genesis. 2018 Sep;56(9):e23238. doi: 10.1002/dvg.23238. PubMed
The large GTPase Mx1 binds Kif5B for cargo transport along microtubules. Ringer K, Riehl J, Muller M, Dewes J, Hoff F, Jacob R. Traffic. 2018 Dec;19(12):947-964. doi: 10.1111/tra.12616. Epub 2018 Oct 23. PubMed
Universal Corneal Epithelial-Like Cells Derived from Human Embryonic Stem Cells for Cellularization of a Corneal Scaffold. Yang J, Park JW, Zheng D, Xu RH. Transl Vis Sci Technol. 2018 Oct 10;7(5):23. doi: 10.1167/tvst.7.5.23. eCollection 2018 Sep. PubMed
REX1 is the critical target of RNF12 in imprinted X chromosome inactivation in mice. Gontan C, Mira-Bontenbal H, Magaraki A, Dupont C, Barakat TS, Rentmeester E, Demmers J, Gribnau J. Nat Commun. 2018 Nov 12;9(1):4752. doi: 10.1038/s41467-018-07060-w. PubMed
SETD3 protein is the actin-specific histidine N-methyltransferase. Kwiatkowski S, Seliga AK, Vertommen D, Terreri M, Ishikawa T, Grabowska I, Tiebe M, Teleman AA, Jagielski AK, Veiga-da-Cunha M, Drozak J. Elife. 2018 Dec 11;7. pii: 37921. doi: 10.7554/eLife.37921. PubMed
FIP200 Claw Domain Binding to p62 Promotes Autophagosome Formation at Ubiquitin Condensates. Turco E, Witt M, Abert C, Bock-Bierbaum T, Su MY, Trapannone R, Sztacho M, Danieli A, Shi X, Zaffagnini G, Gamper A, Schuschnig M, Fracchiolla D, Bernklau D, Romanov J, Hartl M, Hurley JH, Daumke O, Martens S. Mol Cell. 2019 Apr 18;74(2):330-346.e11. doi: 10.1016/j.molcel.2019.01.035. Epub 2019 Mar 7. PubMed
Defined conditions for propagation and manipulation of mouse embryonic stem cells. Mulas C, Kalkan T, von Meyenn F, Leitch HG, Nichols J, Smith A. Development. 2019 Mar 26;146(6). pii: 146/6/dev173146. doi: 10.1242/dev.173146. PubMed
Differential roles of STAT1 and STAT2 in the sensitivity of JAK2V617F- vs. BCR-ABL-positive cells to interferon alpha. Schubert C, Allhoff M, Tillmann S, Maie T, Costa IG, Lipka DB, Schemionek M, Feldberg K, Baumeister J, Brummendorf TH, Chatain N, Koschmieder S. J Hematol Oncol. 2019 Apr 2;12(1):36. doi: 10.1186/s13045-019-0722-9. PubMed
TBX3 represses TBX2 under the control of the PRC2 complex in skeletal muscle and rhabdomyosarcoma. Oh TJ, Adhikari A, Mohamad T, Althobaiti A, Davie J. Oncogenesis. 2019 Apr 12;8(4):27. doi: 10.1038/s41389-019-0137-z. PubMed
Functional characterization of SLC26A3 c.392C>G (p.P131R) mutation in intestinal barrier function using CRISPR/CAS9-created cell models. Zhang N, Heruth DP, Wu W, Zhang LQ, Nsumu MN, Shortt K, Li K, Jiang X, Wang B, Friesen C, Li DY, Ye SQ. Cell Biosci. 2019 May 14;9:40. doi: 10.1186/s13578-019-0303-1. eCollection 2019. PubMed
Hypoosmotic stress induces R loop formation in nucleoli and ATR/ATM-dependent silencing of nucleolar transcription. Velichko AK, Petrova NV, Luzhin AV, Strelkova OS, Ovsyannikova N, Kireev II, Petrova NV, Razin SV, Kantidze OL. Nucleic Acids Res. 2019 Jul 26;47(13):6811-6825. doi: 10.1093/nar/gkz436. PubMed
Inducible Pluripotent Stem Cell-Derived Cardiomyocytes Reveal Aberrant Extracellular Regulated Kinase 5 and Mitogen-Activated Protein Kinase Kinase 1/2 Signaling Concomitantly Promote Hypertrophic Cardiomyopathy in RAF1-Associated Noonan Syndrome. Jaffre F, Miller CL, Schanzer A, Evans T, Roberts AE, Hahn A, Kontaridis MI. Circulation. 2019 Jul 16;140(3):207-224. doi: 10.1161/CIRCULATIONAHA.118.037227. Epub 2019 Jun 5. PubMed
Myosin-II mediated traction forces evoke localized Piezo1-dependent Ca(2+) flickers. Ellefsen KL, Holt JR, Chang AC, Nourse JL, Arulmoli J, Mekhdjian AH, Abuwarda H, Tombola F, Flanagan LA, Dunn AR, Parker I, Pathak MM. Commun Biol. 2019 Aug 7;2:298. doi: 10.1038/s42003-019-0514-3. eCollection 2019. PubMed
Targeted gene therapy in human-induced pluripotent stem cells from a patient with primary hyperoxaluria type 1 using CRISPR/Cas9 technology. Esteve J, Blouin JM, Lalanne M, Azzi-Martin L, Dubus P, Bidet A, Harambat J, Llanas B, Moranvillier I, Bedel A, Moreau-Gaudry F, Richard E. Biochem Biophys Res Commun. 2019 Oct 1;517(4):677-683. doi: 10.1016/j.bbrc.2019.07.109. Epub 2019 Aug 8. PubMed
Type I IFN protects cancer cells from CD8+ T cell-mediated cytotoxicity after radiation. Chen J, Cao Y, Markelc B, Kaeppler J, Vermeer JA, Muschel RJ. J Clin Invest. 2019 Oct 1;129(10):4224-4238. doi: 10.1172/JCI127458. PubMed
Developmental analyses of mouse embryos and adults using a non-overlapping tracing system for all three germ layers. Serizawa T, Isotani A, Matsumura T, Nakanishi K, Nonaka S, Shibata S, Ikawa M, Okano H. Development. 2019 Nov 4;146(21). pii: dev.174938. doi: 10.1242/dev.174938. PubMed

Associated Plasmids

Expression of 9-O- and 7,9-O-Acetyl Modified Sialic Acid in Cells and Their Effects on Influenza Viruses. Barnard KN, Wasik BR, LaClair JR, Buchholz DW, Weichert WS, Alford-Lawrence BK, Aguilar HC, Parrish CR. mBio. 2019 Dec 3;10(6). pii: mBio.02490-19. doi: 10.1128/mBio.02490-19. PubMed
Diminished expression of major histocompatibility complex facilitates the use of human induced pluripotent stem cells in monkey. Wang X, Lu M, Tian X, Ren Y, Li Y, Xiang M, Chen S. Stem Cell Res Ther. 2020 Aug 3;11(1):334. doi: 10.1186/s13287-020-01847-9. PubMed
Deficiency of Grx1 leads to high sensitivity of HeLaS3 cells to oxidative stress via excessive accumulation of intracellular oxidants including ROS. Zhao T, Zhang-Akiyama QM. Free Radic Res. 2020 Sep;54(8-9):585-605. doi: 10.1080/10715762.2020.1819994. Epub 2020 Sep 21. PubMed
STARR-seq identifies active, chromatin-masked, and dormant enhancers in pluripotent mouse embryonic stem cells. Peng T, Zhai Y, Atlasi Y, Ter Huurne M, Marks H, Stunnenberg HG, Megchelenbrink W. Genome Biol. 2020 Sep 10;21(1):243. doi: 10.1186/s13059-020-02156-3. PubMed
PLEKHG5 regulates autophagy, survival and MGMT expression in U251-MG glioblastoma cells. Witte KE, Slotta C, Lutkemeyer M, Kitke A, Coras R, Simon M, Kaltschmidt C, Kaltschmidt B. Sci Rep. 2020 Dec 14;10(1):21858. doi: 10.1038/s41598-020-77958-3. PubMed
miR-379 deletion ameliorates features of diabetic kidney disease by enhancing adaptive mitophagy via FIS1. Kato M, Abdollahi M, Tunduguru R, Tsark W, Chen Z, Wu X, Wang J, Chen ZB, Lin FM, Lanting L, Wang M, Huss J, Fueger PT, Chan D, Natarajan R. Commun Biol. 2021 Jan 4;4(1):30. doi: 10.1038/s42003-020-01516-w. PubMed
Pannexin 1 mutation found in melanoma tumor reduces phosphorylation, glycosylation, and trafficking of the channel-forming protein. Nouri-Nejad D, O'Donnell BL, Patil CS, Sanchez-Pupo RE, Johnston D, Sayedyahossein S, Jurcic K, Lau R, Gyenis L, Litchfield DW, Jackson MF, Gloor GB, Penuela S. Mol Biol Cell. 2021 Mar 1;32(5):376-390. doi: 10.1091/mbc.E19-10-0585. Epub 2021 Jan 6. PubMed
Myogenin is required for assembly of the transcription machinery on muscle genes during skeletal muscle differentiation. Adhikari A, Kim W, Davie J. PLoS One. 2021 Jan 19;16(1):e0245618. doi: 10.1371/journal.pone.0245618. eCollection 2021. PubMed
Spatial regulation of MCAK promotes cell polarization and focal adhesion turnover to drive robust cell migration. Zong H, Hazelbaker M, Moe C, Ems-McClung SC, Hu K, Walczak CE. Mol Biol Cell. 2021 Apr 1;32(7):590-604. doi: 10.1091/mbc.E20-05-0301. Epub 2021 Feb 10. PubMed
Prolyl Oligopeptidase Regulates Dopamine Transporter Oligomerization and Phosphorylation in a PKC- and ERK-Independent Manner. Julku UH, Jantti M, Svarcbahs R, Myohanen TT. Int J Mol Sci. 2021 Feb 10;22(4). pii: ijms22041777. doi: 10.3390/ijms22041777. PubMed
Advanced single-cell pooled CRISPR screening identifies C19orf53 required for cell proliferation based on mTORC1 regulators. Tang Y, Liao S, Liu G, Xiong X, Liu H, Li F, Tan Z, Kong X, Yin Y, Tan B. Cell Biol Toxicol. 2021 Feb 13. pii: 10.1007/s10565-021-09586-0. doi: 10.1007/s10565-021-09586-0. PubMed
TTL-Expression Modulates Epithelial Morphogenesis. Muller M, Ringer K, Hub F, Kamm N, Worzfeld T, Jacob R. Front Cell Dev Biol. 2021 Feb 5;9:635723. doi: 10.3389/fcell.2021.635723. eCollection 2021. PubMed
Pannexin 1 binds beta-catenin to modulate melanoma cell growth and metabolism. Sayedyahossein S, Huang K, Li Z, Zhang C, Kozlov AM, Johnston D, Nouri-Nejad D, Dagnino L, Betts DH, Sacks DB, Penuela S. J Biol Chem. 2021 Jan-Jun;296:100478. doi: 10.1016/j.jbc.2021.100478. Epub 2021 Feb 26. PubMed
Involvement of LH3 and GLT25D1 for glucosyl-galactosyl-hydroxylation on non-collagen-like domain of FGL1. Mori K, Suzuki T, Miura K, Dohmae N, Simizu S. Biochem Biophys Res Commun. 2021 Jun 30;560:93-98. doi: 10.1016/j.bbrc.2021.04.128. Epub 2021 May 10. PubMed
High-throughput screening identifies suppressors of mitochondrial fragmentation in OPA1 fibroblasts. Cretin E, Lopes P, Vimont E, Tatsuta T, Langer T, Gazi A, Sachse M, Yu-Wai-Man P, Reynier P, Wai T. EMBO Mol Med. 2021 Jun 7;13(6):e13579. doi: 10.15252/emmm.202013579. Epub 2021 May 20. PubMed
CRISPR/Cas9 Genome Engineering in Human Pluripotent Stem Cells for Modeling of Neurological Disorders. Canals I, Ahlenius H. Methods Mol Biol. 2021;2352:237-251. doi: 10.1007/978-1-0716-1601-7_16. PubMed

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