pCMV(CAT)T7-SB100 Citations (181)
Originally described in: Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables robust stable gene transfer in vertebrates.Mates L, Chuah MK, Belay E, Jerchow B, Manoj N, Acosta-Sanchez A, Grzela DP, Schmitt A, Becker K, Matrai J, Ma L, Samara-Kuko E, Gysemans C, Pryputniewicz D, Miskey C, Fletcher B, VandenDriessche T, Ivics Z, Izsvak Z Nat Genet. 2009 Jun;41(6):753-61. Epub 2009 May 3. PubMed Journal
Articles Citing pCMV(CAT)T7-SB100
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| Germline transgenesis in rodents by pronuclear microinjection of Sleeping Beauty transposons. Ivics Z, Mates L, Yau TY, Landa V, Zidek V, Bashir S, Hoffmann OI, Hiripi L, Garrels W, Kues WA, Bosze Z, Geurts A, Pravenec M, Rulicke T, Izsvak Z. Nat Protoc. 2014 Apr;9(4):773-93. doi: 10.1038/nprot.2014.008. Epub 2014 Mar 13. PubMed |
| Germline transgenesis in rabbits by pronuclear microinjection of Sleeping Beauty transposons. Ivics Z, Hiripi L, Hoffmann OI, Mates L, Yau TY, Bashir S, Zidek V, Landa V, Geurts A, Pravenec M, Rulicke T, Bosze Z, Izsvak Z. Nat Protoc. 2014 Apr;9(4):794-809. doi: 10.1038/nprot.2014.009. Epub 2014 Mar 13. PubMed |
| Germline transgenesis in pigs by cytoplasmic microinjection of Sleeping Beauty transposons. Ivics Z, Garrels W, Mates L, Yau TY, Bashir S, Zidek V, Landa V, Geurts A, Pravenec M, Rulicke T, Kues WA, Izsvak Z. Nat Protoc. 2014 Apr;9(4):810-27. doi: 10.1038/nprot.2014.010. Epub 2014 Mar 13. PubMed |
| Isolation and cultivation of naive-like human pluripotent stem cells based on HERVH expression. Wang J, Singh M, Sun C, Besser D, Prigione A, Ivics Z, Hurst LD, Izsvak Z. Nat Protoc. 2016 Feb;11(2):327-46. doi: 10.1038/nprot.2016.016. Epub 2016 Jan 21. PubMed |
| Reprogramming of Human Fibroblasts to Induced Pluripotent Stem Cells with Sleeping Beauty Transposon-Based Stable Gene Delivery. Sebe A, Ivics Z. Methods Mol Biol. 2016;1400:419-27. doi: 10.1007/978-1-4939-3372-3_26. PubMed |
| Increased risk of interstitial lung disease in children with a single R288K variant of ABCA3. Wittmann T, Frixel S, Hoppner S, Schindlbeck U, Schams A, Kappler MV, Hegermann J, Wrede C, Liebisch G, Vierzig A, Zacharasiewicz A, Kopp M, Poets CF, Baden W, Hartl D, Van Kaam AH, Lohse P, Aslanidis C, Zarbock R, Griese M. Mol Med. 2016 Feb 26. pii: molmed.2015.00244. doi: 10.2119/molmed.2015.00244. PubMed |
| Sleeping Beauty transposon-based system for rapid generation of HBV-replicating stable cell lines. Wu Y, Zhang TY, Fang LL, Chen ZX, Song LW, Cao JL, Yang L, Yuan Q, Xia NS. J Virol Methods. 2016 Aug;234:96-100. doi: 10.1016/j.jviromet.2016.04.010. Epub 2016 Apr 22. PubMed |
A bacterial phytochrome-based optogenetic system controllable with near-infrared light.
Kaberniuk AA, Shemetov AA, Verkhusha VV.
Nat Methods. 2016 May 9. doi: 10.1038/nmeth.3864.
PubMed
Associated Plasmids |
| Tools to explore ABCA3 mutations causing interstitial lung disease. Wittmann T, Schindlbeck U, Hoppner S, Kinting S, Frixel S, Kroner C, Liebisch G, Hegermann J, Aslanidis C, Brasch F, Reu S, Lasch P, Zarbock R, Griese M. Pediatr Pulmonol. 2016 May 13. doi: 10.1002/ppul.23471. PubMed |
| Efficient generation of transgenic cattle using the DNA transposon and their analysis by next-generation sequencing. Yum SY, Lee SJ, Kim HM, Choi WJ, Park JH, Lee WW, Kim HS, Kim HJ, Bae SH, Lee JH, Moon JY, Lee JH, Lee CI, Son BJ, Song SH, Ji SM, Kim SJ, Jang G. Sci Rep. 2016 Jun 21;6:27185. doi: 10.1038/srep27185. PubMed |
| Enhanced CAR T-cell engineering using non-viral Sleeping Beauty transposition from minicircle vectors. Monjezi R, Miskey C, Gogishvili T, Schleef M, Schmeer M, Einsele H, Ivics Z, Hudecek M. Leukemia. 2017 Jan;31(1):186-194. doi: 10.1038/leu.2016.180. Epub 2016 Jun 24. PubMed |
| Four Novel Splice-Switch Reporter Cell Lines: Distinct Impact of Oligonucleotide Chemistry and Delivery Vector on Biological Activity. Rocha CS, Lundin KE, Behlke MA, Zain R, El Andaloussi S, Smith CI. Nucleic Acid Ther. 2016 Dec;26(6):381-391. Epub 2016 Sep 15. PubMed |
A vector platform for the rapid and efficient engineering of stable complex transgenes.
Jackel C, Nogueira MS, Ehni N, Kraus C, Ranke J, Dohmann M, Noessner E, Nelson PJ.
Sci Rep. 2016 Oct 3;6:34365. doi: 10.1038/srep34365.
PubMed
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| Transposon-mediated Generation of Cellular and Mouse Models of Splicing Mutations to Assess the Efficacy of snRNA-based Therapeutics. Barbon E, Ferrarese M, van Wittenberghe L, Sanatine P, Ronzitti G, Collaud F, Colella P, Pinotti M, Mingozzi F. Mol Ther Nucleic Acids. 2016 Nov 29;5(11):e392. doi: 10.1038/mtna.2016.97. PubMed |
| A Novel Ideal Radionuclide Imaging System for Non-invasively Cell Monitoring built on Baculovirus Backbone by Introducing Sleeping Beauty Transposon. Lv J, Pan Y, Ju H, Zhou J, Cheng D, Shi H, Zhang Y. Sci Rep. 2017 Mar 6;7:43879. doi: 10.1038/srep43879. PubMed |
| Self-organized developmental patterning and differentiation in cerebral organoids. Renner M, Lancaster MA, Bian S, Choi H, Ku T, Peer A, Chung K, Knoblich JA. EMBO J. 2017 May 15;36(10):1316-1329. doi: 10.15252/embj.201694700. Epub 2017 Mar 10. PubMed |
| CRISPR/Cas9-based Pten knock-out and Sleeping Beauty Transposon-mediated Nras knock-in induces hepatocellular carcinoma and hepatic lipid accumulation in mice. Gao M, Liu D. Cancer Biol Ther. 2017 May 17:1-8. doi: 10.1080/15384047.2017.1323597. PubMed |
Guided self-organization and cortical plate formation in human brain organoids.
Lancaster MA, Corsini NS, Wolfinger S, Gustafson EH, Phillips AW, Burkard TR, Otani T, Livesey FJ, Knoblich JA.
Nat Biotechnol. 2017 Jul;35(7):659-666. doi: 10.1038/nbt.3906. Epub 2017 May 31.
PubMed
Associated Plasmids |
| Integration of Oncogenes via Sleeping Beauty as a Mouse Model of HPV16(+) Oral Tumors and Immunologic Control. Lin YH, Yang MC, Tseng SH, Jiang R, Yang A, Farmer E, Peng S, Henkle T, Chang YN, Hung CF, Wu TC. Cancer Immunol Res. 2018 Mar;6(3):305-319. doi: 10.1158/2326-6066.CIR-16-0358. Epub 2018 Jan 23. PubMed |
| Establishment of Cre-mediated HBV recombinant cccDNA (rcccDNA) cell line for cccDNA biology and antiviral screening assays. Wu M, Li J, Yue L, Bai L, Li Y, Chen J, Zhang X, Yuan Z. Antiviral Res. 2018 Apr;152:45-52. doi: 10.1016/j.antiviral.2018.02.007. Epub 2018 Feb 9. PubMed |
| Estrogen-dependent control and cell-to-cell variability of transcriptional bursting. Fritzsch C, Baumgartner S, Kuban M, Steinshorn D, Reid G, Legewie S. Mol Syst Biol. 2018 Feb 23;14(2):e7678. doi: 10.15252/msb.20177678. PubMed |
| Coordinated Control of mRNA and rRNA Processing Controls Embryonic Stem Cell Pluripotency and Differentiation. Corsini NS, Peer AM, Moeseneder P, Roiuk M, Burkard TR, Theussl HC, Moll I, Knoblich JA. Cell Stem Cell. 2018 Apr 5;22(4):543-558.e12. doi: 10.1016/j.stem.2018.03.002. PubMed |
| Novel lymphoid enhancer-binding factor 1-cytoglobin axis promotes extravasation of osteosarcoma cells into the lungs. Pongsuchart M, Kuchimaru T, Yonezawa S, Tran DTP, Kha NT, Hoang NTH, Kadonosono T, Kizaka-Kondoh S. Cancer Sci. 2018 Sep;109(9):2746-2756. doi: 10.1111/cas.13702. Epub 2018 Jul 24. PubMed |
| Genetically engineered cerebral organoids model brain tumor formation. Bian S, Repic M, Guo Z, Kavirayani A, Burkard T, Bagley JA, Krauditsch C, Knoblich JA. Nat Methods. 2018 Aug;15(8):631-639. doi: 10.1038/s41592-018-0070-7. Epub 2018 Jul 23. PubMed |
| A reliable murine model of bone metastasis by injecting cancer cells through caudal arteries. Kuchimaru T, Kataoka N, Nakagawa K, Isozaki T, Miyabara H, Minegishi M, Kadonosono T, Kizaka-Kondoh S. Nat Commun. 2018 Jul 30;9(1):2981. doi: 10.1038/s41467-018-05366-3. PubMed |
| Human iPSC-Derived Natural Killer Cells Engineered with Chimeric Antigen Receptors Enhance Anti-tumor Activity. Li Y, Hermanson DL, Moriarity BS, Kaufman DS. Cell Stem Cell. 2018 Aug 2;23(2):181-192.e5. doi: 10.1016/j.stem.2018.06.002. Epub 2018 Jun 28. PubMed |
| A multi-state model of chemoresistance to characterize phenotypic dynamics in breast cancer. Howard GR, Johnson KE, Rodriguez Ayala A, Yankeelov TE, Brock A. Sci Rep. 2018 Aug 13;8(1):12058. doi: 10.1038/s41598-018-30467-w. PubMed |
| Expression and characterization of soluble epitope-defined major histocompatibility complex (MHC) from stable eukaryotic cell lines. Wooster AL, Anderson TS, Lowe DB. J Immunol Methods. 2019 Jan;464:22-30. doi: 10.1016/j.jim.2018.10.006. Epub 2018 Oct 19. PubMed |
| Purity by design: Reducing impurities in bioproduction by stimulus-controlled global translational downregulation of non-product proteins. Bojar D, Fuhrer T, Fussenegger M. Metab Eng. 2019 Mar;52:110-123. doi: 10.1016/j.ymben.2018.11.007. Epub 2018 Nov 20. PubMed |
| A novel spontaneous hepatocellular carcinoma mouse model for studying T-cell exhaustion in the tumor microenvironment. Liu YT, Tseng TC, Soong RS, Peng CY, Cheng YH, Huang SF, Chuang TH, Kao JH, Huang LR. J Immunother Cancer. 2018 Dec 7;6(1):144. doi: 10.1186/s40425-018-0462-3. PubMed |
| High-performance gene expression and knockout tools using sleeping beauty transposon system. Hu K, Li Y, Wu W, Chen H, Chen Z, Zhang Y, Guo Y, Dong Y. Mob DNA. 2018 Nov 26;9:33. doi: 10.1186/s13100-018-0139-y. eCollection 2018. PubMed |
Mechanical loading of desmosomes depends on the magnitude and orientation of external stress.
Price AJ, Cost AL, Ungewiss H, Waschke J, Dunn AR, Grashoff C.
Nat Commun. 2018 Dec 11;9(1):5284. doi: 10.1038/s41467-018-07523-0.
PubMed
Associated Plasmids |
Creation of versatile cloning platforms for transgene expression and dCas9-based epigenome editing.
Haldeman JM, Conway AE, Arlotto ME, Slentz DH, Muoio DM, Becker TC, Newgard CB.
Nucleic Acids Res. 2018 Dec 27. pii: 5264291. doi: 10.1093/nar/gky1286.
PubMed
Associated Plasmids |
| MicroRNAs reinforce repression of PRC2 transcriptional targets independently and through a feed-forward regulatory network. Shivram H, Le SV, Iyer VR. Genome Res. 2019 Feb;29(2):184-192. doi: 10.1101/gr.238311.118. Epub 2019 Jan 16. PubMed |
| Implantation initiation of self-assembled embryo-like structures generated using three types of mouse blastocyst-derived stem cells. Zhang S, Chen T, Chen N, Gao D, Shi B, Kong S, West RC, Yuan Y, Zhi M, Wei Q, Xiang J, Mu H, Yue L, Lei X, Wang X, Zhong L, Liang H, Cao S, Belmonte JCI, Wang H, Han J. Nat Commun. 2019 Jan 30;10(1):496. doi: 10.1038/s41467-019-08378-9. PubMed |
| Ribosomal Proteins Regulate MHC Class I Peptide Generation for Immunosurveillance. Wei J, Kishton RJ, Angel M, Conn CS, Dalla-Venezia N, Marcel V, Vincent A, Catez F, Ferre S, Ayadi L, Marchand V, Dersh D, Gibbs JS, Ivanov IP, Fridlyand N, Coute Y, Diaz JJ, Qian SB, Staudt LM, Restifo NP, Yewdell JW. Mol Cell. 2019 Mar 21;73(6):1162-1173.e5. doi: 10.1016/j.molcel.2018.12.020. Epub 2019 Jan 31. PubMed |
| Cerebral organoids at the air-liquid interface generate diverse nerve tracts with functional output. Giandomenico SL, Mierau SB, Gibbons GM, Wenger LMD, Masullo L, Sit T, Sutcliffe M, Boulanger J, Tripodi M, Derivery E, Paulsen O, Lakatos A, Lancaster MA. Nat Neurosci. 2019 Apr;22(4):669-679. doi: 10.1038/s41593-019-0350-2. Epub 2019 Mar 18. PubMed |
| A SIINFEKL-Based System to Measure MHC Class I Antigen Presentation Efficiency and Kinetics. Dersh D, Yewdell JW, Wei J. Methods Mol Biol. 2019;1988:109-122. doi: 10.1007/978-1-4939-9450-2_9. PubMed |
| Chromogranin B regulates early-stage insulin granule trafficking from the Golgi in pancreatic islet beta-cells. Bearrows SC, Bauchle CJ, Becker M, Haldeman JM, Swaminathan S, Stephens SB. J Cell Sci. 2019 Jul 1;132(13). pii: jcs.231373. doi: 10.1242/jcs.231373. PubMed |
| Potential limitations of the Sleeping Beauty transposon use in gene expression studies. Sowinska W, Wawro M, Solecka A, Kasza A. Acta Biochim Pol. 2019 Jul 12;66(3):263-268. doi: 10.18388/abp.2019_2839. PubMed |
| Cancer cell population growth kinetics at low densities deviate from the exponential growth model and suggest an Allee effect. Johnson KE, Howard G, Mo W, Strasser MK, Lima EABF, Huang S, Brock A. PLoS Biol. 2019 Aug 5;17(8):e3000399. doi: 10.1371/journal.pbio.3000399. eCollection 2019 Aug. PubMed |
| Elevated UCP1 levels are sufficient to improve glucose uptake in human white adipocytes. Tews D, Pula T, Funcke JB, Jastroch M, Keuper M, Debatin KM, Wabitsch M, Fischer-Posovszky P. Redox Biol. 2019 Sep;26:101286. doi: 10.1016/j.redox.2019.101286. Epub 2019 Jul 27. PubMed |
| Systematic quantification of the anion transport function of pendrin (SLC26A4) and its disease-associated variants. Wasano K, Takahashi S, Rosenberg SK, Kojima T, Mutai H, Matsunaga T, Ogawa K, Homma K. Hum Mutat. 2020 Jan;41(1):316-331. doi: 10.1002/humu.23930. Epub 2019 Oct 26. PubMed |
| Induction of metabolic quiescence defines the transitional to follicular B cell switch. Farmer JR, Allard-Chamard H, Sun N, Ahmad M, Bertocchi A, Mahajan VS, Aicher T, Arnold J, Benson MD, Morningstar J, Barmettler S, Yuen G, Murphy SJH, Walter JE, Ghebremichael M, Shalek AK, Batista F, Gerszten R, Pillai S. Sci Signal. 2019 Oct 22;12(604). pii: 12/604/eaaw5573. doi: 10.1126/scisignal.aaw5573. PubMed |
| Malignant ascites: a source of therapeutic protein against ovarian cancer? Ribaux P, Britan A, Thumann G, Delie F, Petignat P, Cohen M. Oncotarget. 2019 Oct 15;10(57):5894-5905. doi: 10.18632/oncotarget.27185. eCollection 2019 Oct 15. PubMed |
| Mouse Trmt2B protein is a dual specific mitochondrial metyltransferase responsible for m(5)U formation in both tRNA and rRNA. Laptev I, Shvetsova E, Levitskii S, Serebryakova M, Rubtsova M, Bogdanov A, Kamenski P, Sergiev P, Dontsova O. RNA Biol. 2020 Apr;17(4):441-450. doi: 10.1080/15476286.2019.1694733. Epub 2019 Nov 27. PubMed |
| Tumor suppressor properties of the small C-terminal domain phosphatases in non-small cell lung cancer. Krasnov GS, Puzanov GA, Afanasyeva MA, Dashinimaev EB, Vishnyakova KS, Beniaminov AD, Adzhubei AA, Kondratieva TT, Yegorov YE, Senchenko VN. Biosci Rep. 2019 Dec 20;39(12). pii: 221348. doi: 10.1042/BSR20193094. PubMed |
| Individual Cells Can Resolve Variations in Stimulus Intensity along the IGF-PI3K-AKT Signaling Axis. Gross SM, Dane MA, Bucher E, Heiser LM. Cell Syst. 2019 Dec 18;9(6):580-588.e4. doi: 10.1016/j.cels.2019.11.005. Epub 2019 Dec 11. PubMed |
| Proteolytic Processing of Neuregulin 2. Czarnek M, Bereta J. Mol Neurobiol. 2019 Dec 14. pii: 10.1007/s12035-019-01846-9. doi: 10.1007/s12035-019-01846-9. PubMed |
Cell fitness screens reveal a conflict between LINE-1 retrotransposition and DNA replication.
Ardeljan D, Steranka JP, Liu C, Li Z, Taylor MS, Payer LM, Gorbounov M, Sarnecki JS, Deshpande V, Hruban RH, Boeke JD, Fenyo D, Wu PH, Smogorzewska A, Holland AJ, Burns KH.
Nat Struct Mol Biol. 2020 Feb;27(2):168-178. doi: 10.1038/s41594-020-0372-1. Epub 2020 Feb 10.
PubMed
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| CD226(hi)CD8(+) T Cells Are a Prerequisite for Anti-TIGIT Immunotherapy. Jin HS, Ko M, Choi DS, Kim JH, Lee DH, Kang SH, Kim I, Lee HJ, Choi EK, Kim KP, Yoo C, Park Y. Cancer Immunol Res. 2020 Jul;8(7):912-925. doi: 10.1158/2326-6066.CIR-19-0877. Epub 2020 Apr 7. PubMed |
| Bump-and-Hole Engineering Identifies Specific Substrates of Glycosyltransferases in Living Cells. Schumann B, Malaker SA, Wisnovsky SP, Debets MF, Agbay AJ, Fernandez D, Wagner LJS, Lin L, Li Z, Choi J, Fox DM, Peh J, Gray MA, Pedram K, Kohler JJ, Mrksich M, Bertozzi CR. Mol Cell. 2020 Jun 4;78(5):824-834.e15. doi: 10.1016/j.molcel.2020.03.030. Epub 2020 Apr 22. PubMed |
| Cyclin A triggers Mitosis either via the Greatwall kinase pathway or Cyclin B. Hegarat N, Crncec A, Suarez Peredo Rodriguez MF, Echegaray Iturra F, Gu Y, Busby O, Lang PF, Barr AR, Bakal C, Kanemaki MT, Lamond AI, Novak B, Ly T, Hochegger H. EMBO J. 2020 Jun 2;39(11):e104419. doi: 10.15252/embj.2020104419. Epub 2020 Apr 30. PubMed |
| Tracking of an Oral Salmonella-Based Vaccine for Type 1 Diabetes in Non-obese Diabetic Mice. Mbongue JC, Alhoshani A, Rawson J, Garcia PA, Gonzalez N, Ferreri K, Kandeel F, Husseiny MI. Front Immunol. 2020 Apr 28;11:712. doi: 10.3389/fimmu.2020.00712. eCollection 2020. PubMed |
| Prophase-Specific Perinuclear Actin Coordinates Centrosome Separation and Positioning to Ensure Accurate Chromosome Segregation. Stiff T, Echegaray-Iturra FR, Pink HJ, Herbert A, Reyes-Aldasoro CC, Hochegger H. Cell Rep. 2020 May 26;31(8):107681. doi: 10.1016/j.celrep.2020.107681. PubMed |
| MEIS-mediated suppression of human prostate cancer growth and metastasis through HOXB13-dependent regulation of proteoglycans. VanOpstall C, Perike S, Brechka H, Gillard M, Lamperis S, Zhu B, Brown R, Bhanvadia R, Vander Griend DJ. Elife. 2020 Jun 18;9. pii: 53600. doi: 10.7554/eLife.53600. PubMed |
| METTL15 interacts with the assembly intermediate of murine mitochondrial small ribosomal subunit to form m4C840 12S rRNA residue. Laptev I, Shvetsova E, Levitskii S, Serebryakova M, Rubtsova M, Zgoda V, Bogdanov A, Kamenski P, Sergiev P, Dontsova O. Nucleic Acids Res. 2020 Aug 20;48(14):8022-8034. doi: 10.1093/nar/gkaa522. PubMed |
CASC3 promotes transcriptome-wide activation of nonsense-mediated decay by the exon junction complex.
Gerbracht JV, Boehm V, Britto-Borges T, Kallabis S, Wiederstein JL, Ciriello S, Aschemeier DU, Kruger M, Frese CK, Altmuller J, Dieterich C, Gehring NH.
Nucleic Acids Res. 2020 Jul 4. pii: 5867419. doi: 10.1093/nar/gkaa564.
PubMed
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| Adenosine triphosphate binding cassette subfamily C member 1 (ABCC1) overexpression reduces APP processing and increases alpha- versus beta-secretase activity, in vitro. Jepsen WM, De Both M, Siniard AL, Ramsey K, Piras IS, Naymik M, Henderson A, Huentelman MJ. Biol Open. 2021 Jan 25;10(1). pii: bio.054627. doi: 10.1242/bio.054627. PubMed |
| Engineering carotenoid production in mammalian cells for nutritionally enhanced cell-cultured foods. Stout AJ, Mirliani AB, Soule-Albridge EL, Cohen JM, Kaplan DL. Metab Eng. 2020 Nov;62:126-137. doi: 10.1016/j.ymben.2020.07.011. Epub 2020 Sep 2. PubMed |
Optimization of S. aureus dCas9 and CRISPRi Elements for a Single Adeno-Associated Virus that Targets an Endogenous Gene.
Backstrom JR, Sheng J, Wang MC, Bernardo-Colon A, Rex TS.
Mol Ther Methods Clin Dev. 2020 Sep 6;19:139-148. doi: 10.1016/j.omtm.2020.09.001. eCollection 2020 Dec 11.
PubMed
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| Redirecting Vesicular Transport to Improve Nonviral Delivery of Molecular Cargo. Mao M, Chang CC, Pickar-Oliver A, Cervia LD, Wang L, Ji J, Liton PB, Gersbach CA, Yuan F. Adv Biosyst. 2020 Aug;4(8):e2000059. doi: 10.1002/adbi.202000059. Epub 2020 Jul 21. PubMed |
| Recent evolution of a TET-controlled and DPPA3/STELLA-driven pathway of passive DNA demethylation in mammals. Mulholland CB, Nishiyama A, Ryan J, Nakamura R, Yigit M, Gluck IM, Trummer C, Qin W, Bartoschek MD, Traube FR, Parsa E, Ugur E, Modic M, Acharya A, Stolz P, Ziegenhain C, Wierer M, Enard W, Carell T, Lamb DC, Takeda H, Nakanishi M, Bultmann S, Leonhardt H. Nat Commun. 2020 Nov 24;11(1):5972. doi: 10.1038/s41467-020-19603-1. PubMed |
| IRF-1 expressed in the inner cell mass of the porcine early blastocyst enhances the pluripotency of induced pluripotent stem cells. Shi B, Gao D, Zhong L, Zhi M, Weng X, Xu J, Li J, Du X, Xin Y, Gao J, Zhu Q, Cao S, Liu Z, Han J. Stem Cell Res Ther. 2020 Nov 27;11(1):505. doi: 10.1186/s13287-020-01983-2. PubMed |
| CD4 T-cell immune stimulation of HER2 + breast cancer cells alters response to trastuzumab in vitro. Song PN, Mansur A, Dugger KJ, Davis TR, Howard G, Yankeelov TE, Sorace AG. Cancer Cell Int. 2020 Nov 10;20(1):544. doi: 10.1186/s12935-020-01625-w. PubMed |
| Iron overload reduces adiponectin receptor expression via a ROS/FOXO1-dependent mechanism leading to adiponectin resistance in skeletal muscle cells. Dahyaleh K, Sung HK, Prioriello M, Rengasamy P, Lam NH, Kim JB, Gross S, Sweeney G. J Cell Physiol. 2021 Jul;236(7):5339-5351. doi: 10.1002/jcp.30240. Epub 2021 Jan 11. PubMed |
| Functional Evaluation of a Rare Variant c.516G>C (p.Trp172Cys) in the GJB2 (Connexin 26) Gene Associated with Nonsyndromic Hearing Loss. Maslova EA, Orishchenko KE, Posukh OL. Biomolecules. 2021 Jan 5;11(1). pii: biom11010061. doi: 10.3390/biom11010061. PubMed |
| Structurally silent peptide anchor modifications allosterically modulate T cell recognition in a receptor-dependent manner. Smith AR, Alonso JA, Ayres CM, Singh NK, Hellman LM, Baker BM. Proc Natl Acad Sci U S A. 2021 Jan 26;118(4). pii: 2018125118. doi: 10.1073/pnas.2018125118. PubMed |
| Proarrhythmic Electrical Remodeling by Noncardiomyocytes at Interfaces With Cardiomyocytes Under Oxidative Stress. Zhao Y, Iyer S, Tavanaei M, Nguyen NT, Lin A, Nguyen TP. Front Physiol. 2021 Feb 2;11:622613. doi: 10.3389/fphys.2020.622613. eCollection 2020. PubMed |
| HIF1alpha epigenetically repressed macrophages via CRISPR/Cas9-EZH2 system for enhanced cancer immunotherapy. Dong Y, Zhang S, Gao X, Yin D, Wang T, Li Z, Wan Z, Wei M, Luo Y, Yang G, Liu L. Bioact Mater. 2021 Feb 20;6(9):2870-2880. doi: 10.1016/j.bioactmat.2021.02.008. eCollection 2021 Sep. PubMed |
| SARS-CoV-2 seroassay optimization and performance in a population with high background reactivity in Mali. Sagara I, Woodford J, Dicko A, Zeguime A, Doucoure M, Kwan J, Zaidi I, Doritchamou J, Snow-Smith M, Alani N, Renn J, Kosik I, Holly J, Yewdell J, Esposito D, Sadtler K, Duffy P. medRxiv. 2021 Mar 12. doi: 10.1101/2021.03.08.21252784. PubMed |
| NLRC5 regulates expression of MHC-I and provides a target for anti-tumor immunity in transmissible cancers. Ong CEB, Patchett AL, Darby JM, Chen J, Liu GS, Lyons AB, Woods GM, Flies AS. J Cancer Res Clin Oncol. 2021 Jul;147(7):1973-1991. doi: 10.1007/s00432-021-03601-x. Epub 2021 Apr 2. PubMed |
An efficient vector-based CRISPR/Cas9 system in an Oreochromis mossambicus cell line using endogenous promoters.
Hamar J, Kultz D.
Sci Rep. 2021 Apr 12;11(1):7854. doi: 10.1038/s41598-021-87068-3.
PubMed
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| SOD1 regulates ribosome biogenesis in KRAS mutant non-small cell lung cancer. Wang X, Zhang H, Sapio R, Yang J, Wong J, Zhang X, Guo JY, Pine S, Van Remmen H, Li H, White E, Liu C, Kiledjian M, Pestov DG, Steven Zheng XF. Nat Commun. 2021 Apr 15;12(1):2259. doi: 10.1038/s41467-021-22480-x. PubMed |
| Smac mimetics and TRAIL cooperate to induce MLKL-dependent necroptosis in Burkitt's lymphoma cell lines. Koch A, Jeiler B, Roedig J, van Wijk SJL, Dolgikh N, Fulda S. Neoplasia. 2021 May;23(5):539-550. doi: 10.1016/j.neo.2021.03.003. Epub 2021 May 7. PubMed |
| Missense mutations in small muscle protein X-linked (SMPX) cause distal myopathy with protein inclusions. Johari M, Sarparanta J, Vihola A, Jonson PH, Savarese M, Jokela M, Torella A, Piluso G, Said E, Vella N, Cauchi M, Magot A, Magri F, Mauri E, Kornblum C, Reimann J, Stojkovic T, Romero NB, Luque H, Huovinen S, Lahermo P, Donner K, Comi GP, Nigro V, Hackman P, Udd B. Acta Neuropathol. 2021 Aug;142(2):375-393. doi: 10.1007/s00401-021-02319-x. Epub 2021 May 11. PubMed |
One-step multiplex toolkit for efficient generation of conditional gene silencing human cell lines.
Yeung TK, Lau HW, Ma HT, Poon RYC.
Mol Biol Cell. 2021 May 12:mbcE21020051. doi: 10.1091/mbc.E21-02-0051.
PubMed
Associated Plasmids |
| Tumor rejection properties of gp100209-specific T cells correlate with T cell receptor binding affinity towards the wild type rather than anchor-modified antigen. Alonso JA, Smith AR, Baker BM. Mol Immunol. 2021 Jul;135:365-372. doi: 10.1016/j.molimm.2021.05.001. Epub 2021 May 11. PubMed |
| WSX1 act as a tumor suppressor in hepatocellular carcinoma by downregulating neoplastic PD-L1 expression. Wu M, Xia X, Hu J, Fowlkes NW, Li S. Nat Commun. 2021 Jun 9;12(1):3500. doi: 10.1038/s41467-021-23864-9. PubMed |
| Highly-potent, synthetic APOBEC3s restrict HIV-1 through deamination-independent mechanisms. McDonnell MM, Karvonen SC, Gaba A, Flath B, Chelico L, Emerman M. PLoS Pathog. 2021 Jun 25;17(6):e1009523. doi: 10.1371/journal.ppat.1009523. eCollection 2021 Jun. PubMed |
| Development of a Novel Mouse Model of Spontaneous High-Risk HPVE6/E7-Expressing Carcinoma in the Cervicovaginal Tract. Henkle TR, Lam B, Kung YJ, Lin J, Tseng SH, Ferrall L, Xing D, Hung CF, Wu TC. Cancer Res. 2021 Sep 1;81(17):4560-4569. doi: 10.1158/0008-5472.CAN-21-0399. Epub 2021 Jul 2. PubMed |
| Constructing a Smartphone-Controlled Semiautomatic Theranostic System for Glucose Homeostasis in Diabetic Mice. Yu G, Yu Y, Ye H. Methods Mol Biol. 2021;2312:141-158. doi: 10.1007/978-1-0716-1441-9_9. PubMed |
| Identifying oligodendrocyte enhancers governing Plp1 expression. Kim D, An H, Fan C, Park Y. Hum Mol Genet. 2021 Jul 6. pii: 6316230. doi: 10.1093/hmg/ddab184. PubMed |
| alpha-Synuclein fibrils subvert lysosome structure and function for the propagation of protein misfolding between cells through tunneling nanotubes. Dilsizoglu Senol A, Samarani M, Syan S, Guardia CM, Nonaka T, Liv N, Latour-Lambert P, Hasegawa M, Klumperman J, Bonifacino JS, Zurzolo C. PLoS Biol. 2021 Jul 20;19(7):e3001287. doi: 10.1371/journal.pbio.3001287. eCollection 2021 Jul. PubMed |
| Latent TGFbeta-binding proteins regulate UCP1 expression and function via TGFbeta2. Halbgebauer D, Roos J, Funcke JB, Neubauer H, Hamilton BS, Simon E, Amri EZ, Debatin KM, Wabitsch M, Fischer-Posovszky P, Tews D. Mol Metab. 2021 Nov;53:101336. doi: 10.1016/j.molmet.2021.101336. Epub 2021 Sep 1. PubMed |
| Drebrin Regulates Acetylcholine Receptor Clustering and Organization of Microtubules at the Postsynaptic Machinery. Alvarez-Suarez P, Nowak N, Protasiuk-Filipunas A, Yamazaki H, Proszynski TJ, Gawor M. Int J Mol Sci. 2021 Aug 30;22(17). pii: ijms22179387. doi: 10.3390/ijms22179387. PubMed |
| Control of Spontaneous HPV16 E6/E7 Expressing Oral Cancer in HLA-A2 (AAD) Transgenic Mice with Therapeutic HPV DNA Vaccine. Tseng SH, Liu L, Peng S, Kim J, Ferrall L, Hung CF, Wu T-. J Biomed Sci. 2021 Sep 13;28(1):63. doi: 10.1186/s12929-021-00759-x. PubMed |
| Overexpression of lncRNAs with endogenous lengths and functions using a lncRNA delivery system based on transposon. Zhang Y, Huang YX, Jin X, Chen J, Peng L, Wang DL, Li Y, Yao XY, Liao JY, He JH, Hu K, Lu D, Guo Y, Yin D. J Nanobiotechnology. 2021 Oct 2;19(1):303. doi: 10.1186/s12951-021-01044-7. PubMed |
| Non-targeting control for MISSION shRNA library silences SNRPD3 leading to cell death or permanent growth arrest. Czarnek M, Sarad K, Karas A, Kochan J, Bereta J. Mol Ther Nucleic Acids. 2021 Sep 15;26:711-731. doi: 10.1016/j.omtn.2021.09.004. eCollection 2021 Dec 3. PubMed |
| Variations in cell-surface ACE2 levels alter direct binding of SARS-CoV-2 Spike protein and viral infectivity: Implications for measuring Spike protein interactions with animal ACE2 orthologs. Kazemi S, Lopez-Munoz AD, Holly J, Jin L, Yewdell JW, Dolan BP. bioRxiv. 2021 Oct 22. doi: 10.1101/2021.10.21.465386. PubMed |
| Here and there: the double-side transgene localization. Salnikov PA, Khabarova AA, Koksharova GS, Mungalov RV, Belokopytova PS, Pristyazhnuk IE, Nurislamov AR, Somatich P, Gridina MM, Fishman VS. Vavilovskii Zhurnal Genet Selektsii. 2021 Oct;25(6):607-612. doi: 10.18699/VJ21.068. PubMed |
| Therapeutic potential of parkin as a tumor suppressor via transcriptional control of cyclins in glioblastoma cell and animal models. Rouland L, Duplan E, Ramos Dos Santos L, Bernardin A, Katula KS, Manfioletti G, Idbaih A, Checler F, Alves da Costa C. Theranostics. 2021 Nov 1;11(20):10047-10063. doi: 10.7150/thno.57549. eCollection 2021. PubMed |
| IPSC-Derived Human Neurons with GCaMP6s Expression Allow In Vitro Study of Neurophysiological Responses to Neurochemicals. Galiakberova AA, Surin AM, Bakaeva ZV, Sharipov RR, Zhang D, Dorovskoy DA, Shakirova KM, Fisenko AP, Dashinimaev EB. Neurochem Res. 2022 Apr;47(4):952-966. doi: 10.1007/s11064-021-03497-6. Epub 2021 Dec 2. PubMed |
| A single intranasal dose of a live-attenuated parainfluenza virus-vectored SARS-CoV-2 vaccine is protective in hamsters. Liu X, Luongo C, Matsuoka Y, Park HS, Santos C, Yang L, Moore IN, Afroz S, Johnson RF, Lafont BAP, Martens C, Best SM, Munster VJ, Holly J, Yewdell JW, Le Nouen C, Munir S, Buchholz UJ. Proc Natl Acad Sci U S A. 2021 Dec 14;118(50):e2109744118. doi: 10.1073/pnas.2109744118. PubMed |
| A targetable LIFR-NF-kappaB-LCN2 axis controls liver tumorigenesis and vulnerability to ferroptosis. Yao F, Deng Y, Zhao Y, Mei Y, Zhang Y, Liu X, Martinez C, Su X, Rosato RR, Teng H, Hang Q, Yap S, Chen D, Wang Y, Chen MM, Zhang M, Liang H, Xie D, Chen X, Zhu H, Chang JC, You MJ, Sun Y, Gan B, Ma L. Nat Commun. 2021 Dec 17;12(1):7333. doi: 10.1038/s41467-021-27452-9. PubMed |
| Cell Surface SARS-CoV-2 Nucleocapsid Protein Modulates Innate and Adaptive Immunity. Lopez-Munoz AD, Kosik I, Holly J, Yewdell JW. bioRxiv. 2021 Dec 13. doi: 10.1101/2021.12.10.472169. PubMed |
| Multifunctional barcoding with ClonMapper enables high-resolution study of clonal dynamics during tumor evolution and treatment. Gutierrez C, Al'Khafaji AM, Brenner E, Johnson KE, Gohil SH, Lin Z, Knisbacher BA, Durrett RE, Li S, Parvin S, Biran A, Zhang W, Rassenti L, Kipps TJ, Livak KJ, Neuberg D, Letai A, Getz G, Wu CJ, Brock A. Nat Cancer. 2021 Jul;2(7):758-772. doi: 10.1038/s43018-021-00222-8. Epub 2021 Jul 12. PubMed |
| Signaling by the tyrosine kinase Yes promotes liver cancer development. Guegan JP, Lapouge M, Voisin L, Saba-El-Leil MK, Tanguay PL, Levesque K, Bregeon J, Mes-Masson AM, Lamarre D, Haibe-Kains B, Trinh VQ, Soucy G, Bilodeau M, Meloche S. Sci Signal. 2022 Jan 18;15(717):eabj4743. doi: 10.1126/scisignal.abj4743. Epub 2022 Jan 18. PubMed |
| SOX2 mediates metabolic reprogramming of prostate cancer cells. de Wet L, Williams A, Gillard M, Kregel S, Lamperis S, Gutgesell LC, Vellky JE, Brown R, Conger K, Paner GP, Wang H, Platz EA, De Marzo AM, Mu P, Coloff JL, Szmulewitz RZ, Vander Griend DJ. Oncogene. 2022 Feb;41(8):1190-1202. doi: 10.1038/s41388-021-02157-x. Epub 2022 Jan 24. PubMed |
| Genomic context sensitivity of insulator function. Ribeiro-Dos-Santos AM, Hogan MS, Luther RD, Brosh R, Maurano MT. Genome Res. 2022 Mar;32(3):425-436. doi: 10.1101/gr.276449.121. Epub 2022 Jan 26. PubMed |
| In vivo CRISPR screening identifies BAZ2 chromatin remodelers as druggable regulators of mammalian liver regeneration. Jia Y, Li L, Lin YH, Gopal P, Shen S, Zhou K, Yu X, Sharma T, Zhang Y, Siegwart DJ, Ready JM, Zhu H. Cell Stem Cell. 2022 Mar 3;29(3):372-385.e8. doi: 10.1016/j.stem.2022.01.001. Epub 2022 Jan 31. PubMed |
| shRNAs targeting mouse Adam10 diminish cell response to proinflammatory stimuli independently of Adam10 silencing. Czarnek M, Stalinska K, Sarad K, Bereta J. Biol Open. 2022 Mar 15;11(3). pii: 274200. doi: 10.1242/bio.059092. Epub 2022 Mar 4. PubMed |
| Transposon-mediated glial cell line-derived neurotrophic factor overexpression in human adipose tissue-derived mesenchymal stromal cells: A potential approach for neuroregenerative medicine? Rasinska J, Klein C, Stahn L, Maidhof F, Pfeffer A, Schreyer S, Gossen M, Kurtz A, Steiner B, Hemmati-Sadeghi S. J Tissue Eng Regen Med. 2022 Jun;16(6):515-529. doi: 10.1002/term.3296. Epub 2022 Mar 12. PubMed |
| Dominant negative effects of SCN5A missense variants. O'Neill MJ, Muhammad A, Li B, Wada Y, Hall L, Solus JF, Short L, Roden DM, Glazer AM. Genet Med. 2022 Jun;24(6):1238-1248. doi: 10.1016/j.gim.2022.02.010. Epub 2022 Mar 16. PubMed |
| A Dual-Reporter Platform for Screening Tumor-Targeted Extracellular Vesicles. Kanada M, Linenfelser L, Cox E, Gilad AA. Pharmaceutics. 2022 Feb 22;14(3):475. doi: 10.3390/pharmaceutics14030475. PubMed |
| Quantification of long-term doxorubicin response dynamics in breast cancer cell lines to direct treatment schedules. Howard GR, Jost TA, Yankeelov TE, Brock A. PLoS Comput Biol. 2022 Mar 31;18(3):e1009104. doi: 10.1371/journal.pcbi.1009104. eCollection 2022 Mar. PubMed |
| Fibroblast growth factor 19 stimulates water intake. Ursic-Bedoya J, Chavey C, Desandre G, Meunier L, Dupuy AM, Gonzalez-Dopeso Reyes I, Tordjmann T, Assenat E, Hibner U, Gregoire D. Mol Metab. 2022 Jun;60:101483. doi: 10.1016/j.molmet.2022.101483. Epub 2022 Mar 31. PubMed |
| Targeted Transposition of Minicircle DNA Using Single-Chain Antibody Conjugated Cyclodextrin-Modified Poly (Propylene Imine) Nanocarriers. Jugel W, Tietze S, Daeg J, Appelhans D, Broghammer F, Aigner A, Karimov M, Schackert G, Temme A. Cancers (Basel). 2022 Apr 11;14(8). pii: cancers14081925. doi: 10.3390/cancers14081925. PubMed |
| Application of transposon systems in the transgenesis of bovine somatic and germ cells. Kwon DH, Gim GM, Eom KH, Lee JH, Jang G. BMC Vet Res. 2022 Apr 27;18(1):156. doi: 10.1186/s12917-022-03252-1. PubMed |
| Generation of Mature DENVs via Genetic Modification and Directed Evolution. Tse LV, Meganck RM, Dong S, Adams LE, White LJ, Mallory ML, Jadi R, de Silva AM, Baric RS. mBio. 2022 Jun 28;13(3):e0038622. doi: 10.1128/mbio.00386-22. Epub 2022 Apr 28. PubMed |
| Nonlinear mechanics of human mitotic chromosomes. Meijering AEC, Sarlos K, Nielsen CF, Witt H, Harju J, Kerklingh E, Haasnoot GH, Bizard AH, Heller I, Broedersz CP, Liu Y, Peterman EJG, Hickson ID, Wuite GJL. Nature. 2022 May;605(7910):545-550. doi: 10.1038/s41586-022-04666-5. Epub 2022 May 4. PubMed |
| Molecular Subgroups of Intrahepatic Cholangiocarcinoma Discovered by Single-Cell RNA Sequencing-Assisted Multiomics Analysis. Bao X, Li Q, Chen J, Chen D, Ye C, Dai X, Wang Y, Li X, Rong X, Cheng F, Jiang M, Zhu Z, Ding Y, Sun R, Liu C, Huang L, Jin Y, Li B, Lu J, Wu W, Guo Y, Fu W, Langley SR, Tano V, Fang W, Guo T, Sheng J, Zhao P, Ruan J. Cancer Immunol Res. 2022 Jul 1;10(7):811-828. doi: 10.1158/2326-6066.CIR-21-1101. PubMed |
| Engineering Functional Membrane-Membrane Interfaces by InterSpy. Moghimianavval H, Patel C, Mohapatra S, Hwang SW, Kayikcioglu T, Bashirzadeh Y, Liu AP, Ha T. Small. 2022 May 26:e2202104. doi: 10.1002/smll.202202104. PubMed |
MicroID2: A Novel Biotin Ligase Enables Rapid Proximity Dependent Proteomics.
Johnson BS, Chafin L, Farkas D, Adair J, Elhance A, Farkas L, Bednash JS, Londino JD.
Mol Cell Proteomics. 2022 Jun 7:100256. doi: 10.1016/j.mcpro.2022.100256.
PubMed
Associated Plasmids |
| Increased LAMP1 Expression Enhances SARS-CoV-1 and SARS-CoV-2 Production in Vero-Derived Transgenic Cell Lines. Dolskiy AA, Grishchenko IV, Bodnev SA, Nazarenko AA, Smirnova AM, Matveeva AK, Bulychev LE, Ovchinnikova AS, Tregubchak TV, Zaykovskaya AV, Imatdinov IR, Pyankov OV, Gavrilova EV, Maksyutov RA, Yudkin DV. Mol Biol. 2022;56(3):463-468. doi: 10.1134/S0026893322030050. Epub 2022 Jun 3. PubMed |
| Novel Paired Cell Lines for the Study of Lipid Metabolism and Cancer Stemness of Hepatocellular Carcinoma. Cheng YH, Ko YC, Ku HJ, Huang CC, Yao YC, Liao YT, Chen YT, Huang SF, Huang LR. Front Cell Dev Biol. 2022 May 26;10:821224. doi: 10.3389/fcell.2022.821224. eCollection 2022. PubMed |
| Hepatocyte growth factor derived from senescent cells attenuates cell competition-induced apical elimination of oncogenic cells. Igarashi N, Miyata K, Loo TM, Chiba M, Hanyu A, Nishio M, Kawasaki H, Zheng H, Toyokuni S, Kon S, Moriyama K, Fujita Y, Takahashi A. Nat Commun. 2022 Jul 18;13(1):4157. doi: 10.1038/s41467-022-31642-4. PubMed |
| Compilation and evaluation of a fatty acid mimetics screening library. Ehrler JHM, Brunst S, Tjaden A, Kilu W, Heering J, Hernandez-Olmos V, Krommes A, Kramer JS, Steinhilber D, Schubert-Zsilavecz M, Muller S, Merk D, Proschak E. Biochem Pharmacol. 2022 Oct;204:115191. doi: 10.1016/j.bcp.2022.115191. Epub 2022 Jul 27. PubMed |
| Cell surface SARS-CoV-2 nucleocapsid protein modulates innate and adaptive immunity. Lopez-Munoz AD, Kosik I, Holly J, Yewdell JW. Sci Adv. 2022 Aug 5;8(31):eabp9770. doi: 10.1126/sciadv.abp9770. Epub 2022 Aug 3. PubMed |
| Identification of Estradiol Benzoate as an Inhibitor of HBx Using Inducible Stably Transfected HepG2 Cells Expressing HiBiT Tagged HBx. He J, Wu J, Chen J, Zhang S, Guo Y, Zhang X, Han J, Zhang Y, Guo Y, Lin Y, Yu W, Kong Y, Shen Z, Mao R, Zhang J. Molecules. 2022 Aug 6;27(15):5000. doi: 10.3390/molecules27155000. PubMed |
| The human EF1a promoter does not provide expression of the transgene in mice. Battulin N, Korablev A, Ryzhkova A, Smirnov A, Kabirova E, Khabarova A, Lagunov T, Serova I, Serov O. Transgenic Res. 2022 Oct;31(4-5):525-535. doi: 10.1007/s11248-022-00319-5. Epub 2022 Aug 12. PubMed |
| Targeting the mRNA endonuclease CPSF73 inhibits breast cancer cell migration, invasion, and self-renewal. Liu H, Heller-Trulli D, Moore CL. iScience. 2022 Jul 20;25(8):104804. doi: 10.1016/j.isci.2022.104804. eCollection 2022 Aug 19. PubMed |
| Variations in Cell Surface ACE2 Levels Alter Direct Binding of SARS-CoV-2 Spike Protein and Viral Infectivity: Implications for Measuring Spike Protein Interactions with Animal ACE2 Orthologs. Kazemi S, Lopez-Munoz AD, Holly J, Jin L, Yewdell JW, Dolan BP. J Virol. 2022 Sep 14;96(17):e0025622. doi: 10.1128/jvi.00256-22. Epub 2022 Aug 24. PubMed |
| Arc Regulates Transcription of Genes for Plasticity, Excitability and Alzheimer's Disease. Leung HW, Foo G, VanDongen A. Biomedicines. 2022 Aug 11;10(8):1946. doi: 10.3390/biomedicines10081946. PubMed |
| Quantifying the Effects of Combination Trastuzumab and Radiation Therapy in Human Epidermal Growth Factor Receptor 2-Positive Breast Cancer. Bloom MJ, Song PN, Virostko J, Yankeelov TE, Sorace AG. Cancers (Basel). 2022 Aug 31;14(17):4234. doi: 10.3390/cancers14174234. PubMed |
| Quantitative fragmentomics allow affinity mapping of interactomes. Gogl G, Zambo B, Kostmann C, Cousido-Siah A, Morlet B, Durbesson F, Negroni L, Eberling P, Jane P, Nomine Y, Zeke A, Ostergaard S, Monsellier E, Vincentelli R, Trave G. Nat Commun. 2022 Sep 17;13(1):5472. doi: 10.1038/s41467-022-33018-0. PubMed |
| Nuclear receptor ligand screening in an iPSC-derived in vitro blood-brain barrier model identifies new contributors to leptin transport. Shi Y, Kim H, Hamann CA, Rhea EM, Brunger JM, Lippmann ES. Fluids Barriers CNS. 2022 Sep 21;19(1):77. doi: 10.1186/s12987-022-00375-3. PubMed |
| Targeting the Phosphatidylserine-Immune Checkpoint with a Small-Molecule Maytansinoid Conjugate. Lo CF, Chiu TY, Liu YT, Pan PY, Liu KL, Hsu CY, Fang MY, Huang YC, Yeh TK, Hsu TA, Chen CT, Huang LR, Tsou LK. J Med Chem. 2022 Oct 13;65(19):12802-12824. doi: 10.1021/acs.jmedchem.2c00631. Epub 2022 Sep 26. PubMed |
| PtdIns(3,4)P2, Lamellipodin, and VASP coordinate actin dynamics during phagocytosis in macrophages. Montano-Rendon F, Walpole GFW, Krause M, Hammond GRV, Grinstein S, Fairn GD. J Cell Biol. 2022 Nov 7;221(11):e202207042. doi: 10.1083/jcb.202207042. Epub 2022 Sep 27. PubMed |
| Identifying an oligodendrocyte enhancer that regulates Olig2 expression. Fan C, Kim D, An H, Park Y. Hum Mol Genet. 2023 Feb 19;32(5):835-846. doi: 10.1093/hmg/ddac249. PubMed |
| Class II transactivator induces expression of MHC-I and MHC-II in transmissible Tasmanian devil facial tumours. Ong CEB, Cheng Y, Siddle HV, Lyons AB, Woods GM, Flies AS. Open Biol. 2022 Oct;12(10):220208. doi: 10.1098/rsob.220208. Epub 2022 Oct 19. PubMed |
| Cell-specific bioorthogonal tagging of glycoproteins. Cioce A, Calle B, Rizou T, Lowery SC, Bridgeman VL, Mahoney KE, Marchesi A, Bineva-Todd G, Flynn H, Li Z, Tastan OY, Roustan C, Soro-Barrio P, Rafiee MR, Garza-Garcia A, Antonopoulos A, Wood TM, Keenan T, Both P, Huang K, Parmeggian F, Snijders AP, Skehel M, Kjaer S, Fascione MA, Bertozzi CR, Haslam SM, Flitsch SL, Malaker SA, Malanchi I, Schumann B. Nat Commun. 2022 Oct 25;13(1):6237. doi: 10.1038/s41467-022-33854-0. PubMed |
| Using the Sleeping Beauty (SB) Transposon to Generate Stable Cells Producing Enveloped Virus-Like Particles (eVLPs) Pseudotyped with SARS-CoV-2 Proteins for Vaccination. Pszenny V, Tjhin E, Alves-Ferreira EVC, Spada S, Bouamr F, Nair V, Ganesan S, Grigg ME. Curr Protoc. 2022 Oct;2(10):e575. doi: 10.1002/cpz1.575. PubMed |
| Single-base resolution mapping of 2'-O-methylation sites by an exoribonuclease-enriched chemical method. Zhang P, Huang J, Zheng W, Chen L, Liu S, Liu A, Ye J, Zhou J, Chen Z, Huang Q, Liu S, Zhou K, Qu L, Li B, Yang J. Sci China Life Sci. 2023 Apr;66(4):800-818. doi: 10.1007/s11427-022-2210-0. Epub 2022 Oct 28. PubMed |
| MARCH5 regulates mitotic apoptosis through MCL1-dependent and independent mechanisms. Wang Y, Poon RYC. Cell Death Differ. 2023 Mar;30(3):753-765. doi: 10.1038/s41418-022-01080-2. Epub 2022 Nov 3. PubMed |
| A lineage tree-based hidden Markov model quantifies cellular heterogeneity and plasticity. Mohammadi F, Visagan S, Gross SM, Karginov L, Lagarde JC, Heiser LM, Meyer AS. Commun Biol. 2022 Nov 17;5(1):1258. doi: 10.1038/s42003-022-04208-9. PubMed |
| Structural basis for activation of DNMT1. Kikuchi A, Onoda H, Yamaguchi K, Kori S, Matsuzawa S, Chiba Y, Tanimoto S, Yoshimi S, Sato H, Yamagata A, Shirouzu M, Adachi N, Sharif J, Koseki H, Nishiyama A, Nakanishi M, Defossez PA, Arita K. Nat Commun. 2022 Nov 21;13(1):7130. doi: 10.1038/s41467-022-34779-4. PubMed |
| Structural basis for the unique multifaceted interaction of DPPA3 with the UHRF1 PHD finger. Hata K, Kobayashi N, Sugimura K, Qin W, Haxholli D, Chiba Y, Yoshimi S, Hayashi G, Onoda H, Ikegami T, Mulholland CB, Nishiyama A, Nakanishi M, Leonhardt H, Konuma T, Arita K. Nucleic Acids Res. 2022 Nov 28;50(21):12527-12542. doi: 10.1093/nar/gkac1082. PubMed |
| Intravital electrochemical nanosensor as a tool for the measurement of reactive oxygen/nitrogen species in liver diseases. Abakumova T, Vaneev A, Naumenko V, Shokhina A, Belousov V, Mikaelyan A, Balysheva K, Gorelkin P, Erofeev A, Zatsepin T. J Nanobiotechnology. 2022 Nov 24;20(1):497. doi: 10.1186/s12951-022-01688-z. PubMed |
| Construction of a Versatile, Programmable RNA-Binding Protein Using Designer PPR Proteins and Its Application for Splicing Control in Mammalian Cells. Yagi Y, Teramoto T, Kaieda S, Imai T, Sasaki T, Yagi M, Maekawa N, Nakamura T. Cells. 2022 Nov 8;11(22):3529. doi: 10.3390/cells11223529. PubMed |
| Pentatricopeptide Protein PTCD2 Regulates COIII Translation in Mitochondria of the HeLa Cell Line. Baleva MV, Chicherin I, Piunova U, Zgoda V, Patrushev MV, Levitskii S, Kamenski P. Int J Mol Sci. 2022 Nov 17;23(22):14241. doi: 10.3390/ijms232214241. PubMed |
| SRSF3 and HNRNPH1 Regulate Radiation-Induced Alternative Splicing of Protein Arginine Methyltransferase 5 in Hepatocellular Carcinoma. Wen C, Tian Z, Li L, Chen T, Chen H, Dai J, Liang Z, Ma S, Liu X. Int J Mol Sci. 2022 Nov 27;23(23):14832. doi: 10.3390/ijms232314832. PubMed |
| In vitro genome editing activity of Cas9 in somatic cells after random and transposon-based genomic Cas9 integration. Sollner JH, Sake HJ, Frenzel A, Lechler R, Herrmann D, Fuchs W, Petersen B. PLoS One. 2022 Dec 30;17(12):e0279123. doi: 10.1371/journal.pone.0279123. eCollection 2022. PubMed |
Stable production of recombinant SARS-CoV-2 receptor-binding domain in mammalian cells with co-expression of a fluorescent reporter and its validation as antigenic target for COVID-19 serology testing.
Arias-Arias JL, Molina-Castro SE, Monturiol-Gross L, Lomonte B, Corrales-Aguilar E.
Biotechnol Rep (Amst). 2023 Mar;37:e00780. doi: 10.1016/j.btre.2022.e00780. Epub 2022 Dec 31.
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| Bump-and-hole engineering of human polypeptide N-acetylgalactosamine transferases to dissect their protein substrates and glycosylation sites in cells. Calle B, Gonzalez-Rodriguez E, Mahoney KE, Cioce A, Bineva-Todd G, Tastan OY, Roustan C, Flynn H, Malaker SA, Schumann B. STAR Protoc. 2023 Jan 11;4(1):101974. doi: 10.1016/j.xpro.2022.101974. PubMed |
| The interferon stimulated gene-encoded protein HELZ2 inhibits human LINE-1 retrotransposition and LINE-1 RNA-mediated type I interferon induction. Luqman-Fatah A, Watanabe Y, Uno K, Ishikawa F, Moran JV, Miyoshi T. Nat Commun. 2023 Jan 13;14(1):203. doi: 10.1038/s41467-022-35757-6. PubMed |
| AMPK is required for recovery from metabolic stress induced by ultrasound microbubble treatment. Lo L, Uchenunu O, Botelho RJ, Antonescu CN, Karshafian R. iScience. 2022 Dec 28;26(2):105883. doi: 10.1016/j.isci.2022.105883. eCollection 2023 Feb 17. PubMed |
Construction of a Set of Novel Transposon Vectors for Efficient Silencing of Protein and lncRNA Genes via CRISPR Interference.
Czarnek M, Kochan J, Wawro M, Myrczek R, Bereta J.
Mol Biotechnol. 2023 Jan 28. doi: 10.1007/s12033-023-00675-5.
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| Cyclin A-CDK1 suppresses the expression of the CDK1 activator CDC25A to safeguard timely mitotic entry. Ng LY, Ma HT, Poon RYC. J Biol Chem. 2023 Mar;299(3):102957. doi: 10.1016/j.jbc.2023.102957. Epub 2023 Jan 28. PubMed |
| Inhibiting the Deubiquitinase UCHL1 Reduces SARS-CoV-2 Viral Uptake by ACE2. Bednash JS, Johns F, Farkas D, Elhance A, Adair J, Cress K, Yount JS, Kenney AD, Londino JD, Mallampalli RK. Am J Respir Cell Mol Biol. 2023 May;68(5):566-576. doi: 10.1165/rcmb.2022-0331OC. PubMed |
| Androgen receptor variant 7 exacerbates hepatocarcinogenesis in a c-MYC-driven mouse HCC model. Kido T, Lau YC. Oncogenesis. 2023 Feb 6;12(1):4. doi: 10.1038/s41389-023-00449-3. PubMed |
| The homeostatic function of Regnase-2 restricts neuroinflammation. Sowinska W, Wawro M, Biswas DD, Kochan J, Pustelny K, Solecka A, Gupta AS, Mockenhaupt K, Polak J, Kwinta B, Kordula T, Kasza A. FASEB J. 2023 Mar;37(3):e22798. doi: 10.1096/fj.202201978R. PubMed |
| Myelin Basic Protein Fragmentation by Engineered Human Proteasomes with Different Catalytic Phenotypes Revealed Direct Peptide Ligands of MS-Associated and Protective HLA Class I Molecules. Saratov GA, Vladimirov VI, Novoselov AL, Ziganshin RH, Chen G, Baymukhametov TN, Konevega AL, Belogurov AA Jr, Kudriaeva AA. Int J Mol Sci. 2023 Jan 20;24(3):2091. doi: 10.3390/ijms24032091. PubMed |
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