Self-Reporting Transposons Enable Simultaneous Readout of Gene Expression and Transcription Factor Binding in Single Cells.
Moudgil A, Wilkinson MN, Chen X, He J, Cammack AJ, Vasek MJ, Lagunas T Jr, Qi Z, Lalli MA, Guo C, Morris SA, Dougherty JD, Mitra RD
Cell. 2020 Aug 20;182(4):992-1008.e21. doi: 10.1016/j.cell.2020.06.037. Epub 2020 Jul 24. PubMed Article
Moudgil A, Wilkinson MN, Chen X, He J, Cammack AJ, Vasek MJ, Lagunas T Jr, Qi Z, Lalli MA, Guo C, Morris SA, Dougherty JD, Mitra RD
Cell. 2020 Aug 20;182(4):992-1008.e21. doi: 10.1016/j.cell.2020.06.037. Epub 2020 Jul 24. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 154884 | PB-SRT-Puro | piggyBac self-reporting transposon with puromycin marker for mammalian calling cards |
| 154885 | PB-SRT-tdTomato | piggyBac self-reporting transposon with tdTomato marker for mammalian calling cards |
| 154887 | SP1-SB100X | Sleeping Beauty transposase fused to SP1 |
| 154888 | SB-SRT-Puro | Sleeping Beauty self-reporting transposon with puromycin reporter |
| 154890 | BRD4 CRISPRi plasmid | Human BRD4 CRISPRi gRNA |