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Addgene
One-step multiplex toolkit for efficient generation of conditional gene silencing human cell lines.
Yeung TK, Lau HW, Ma HT, Poon RYC
Mol Biol Cell. 2021 May 12:mbcE21020051. doi: 10.1091/mbc.E21-02-0051. PubMed Article

Plasmids from Article

ID Plasmid Purpose
171677pUHD-SB-AID/HygTRE-controlled AID-fusion sleeping beauty transposon vector with selection marker (hygromycin)
171678pUHD-SB-AID/BlaTRE-controlled AID-fusion sleeping beauty transposon vector with selection marker (blasticidin)
171679pUHD-SB-mAID/HygTRE-controlled mAID-fusion sleeping beauty transposon vector with selection marker (hygromycin)
171680pUHD-SB-mAID/BlaTRE-controlled mAID-fusion sleeping beauty transposon vector with selection marker (blasticidin)
171681pUHD-SB-mAID/ZeoTRE-controlled mAID-fusion sleeping beauty transposon vector with selection marker (zeocin)
171682pSBbi-TIR1/Purconstitutive expression of TIR1-myc in sleeping beauty transposon vector with selection marker (puromycin)
171683pSBbi-TIR1-tTA/Purconstitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (puromycin)
171684pSBbi-TIR1-tTA/Neoconstitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (G418)
171685pSBbi-TIR1-tTA/Zeoconstitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (zeocin)

Antibodies from Article