pT2-CAG-fGFP
(Plasmid #108714)

• Purpose: Transposon donor plasmid for long-term expression of membrane targetted GFP
• Depositing Lab: Madeline Lancaster
• Depositing Organization: MRC Laboratory of Molecular Biology
  Located in the United Kingdom of Great Britain and Northern Ireland
• Publication: Lancaster et al Nat Biotechnol. 2017 Jul;35(7):659-666. doi: 10.1038/nbt.3906. Epub 2017 May 31.
• Citations:

  5 Articles

Backbone

• Vector backbone: pT2HB
• Backbone manufacturer: Perry Hackett (Addgene plasmid 26557)
• Modifications to backbone: This plasmid was modified so that it included additional restriction sites and attR sites that would allow the insertion of sequences using Gateway cloning.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EGFP
• Species: Synthetic; Aequorea victoria (Jellyfish)
• Promoter: CAG
• Tags / Fusion Proteins:
  • farnesylation signal (C terminal on insert)
  • 6xHis (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The source plasmid with GFP was a gift from Safa Lucken-Ardjomande Häsler (member of the Harvey McMahon group at the MRC LMB). Dr. Häsler cloned the gene from the pEGFP-N3 plasmid, originally received from Clontech.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

DNA (Catalog # 108714-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 108714-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.