Construction of a Set of Novel Transposon Vectors for Efficient Silencing of Protein and lncRNA Genes via CRISPR Interference.
Czarnek M, Kochan J, Wawro M, Myrczek R, Bereta J
Mol Biotechnol. 2023 Jan 28. doi: 10.1007/s12033-023-00675-5. PubMed Article
Czarnek M, Kochan J, Wawro M, Myrczek R, Bereta J
Mol Biotechnol. 2023 Jan 28. doi: 10.1007/s12033-023-00675-5. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 196074 | pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-SapI | (Empty Backbone) Constitutive CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites. |
| 196075 | pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-A10 | Constitutive CRISPRi vector conferring blasticidin S resistance, expressing gRNA targeting mouse Adam10. |
| 196076 | pSBbi-Hyg-dCas9-KRAB-MeCP2_hU6-SapI | (Empty Backbone) Constitutive CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites. |
| 196077 | pSBbi-Hyg-dCas9-KRAB-MeCP2_hU6-A10 | Constitutive CRISPRi vector conferring hygromycin B resistance, expressing gRNA targeting mouse Adam10. |
| 196078 | pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-SapI | (Empty Backbone) Constitutive CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites. |
| 196079 | pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-A10 | Constitutive CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10. |
| 196080 | pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-SapI | (Empty Backbone) Inducible CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites. |
| 196081 | pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-A10 | Inducible CRISPRi vector conferring blasticidin S resistance, expressing gRNA targeting mouse Adam10. |
| 196082 | pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-SapI | (Empty Backbone) Inducible CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites. |
| 196083 | pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-A10 | Inducible CRISPRi vector conferring hygromycin B resistance, expressing gRNA targeting mouse Adam10. |
| 196084 | pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-SapI | (Empty Backbone) Inducible CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites. |
| 196085 | pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-A10 | Inducible CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10. |
| 196086 | pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-NORAD | Inducible CRISPRi vector conferring puromycin resistance, expressing gRNA targeting lncRNA NORAD. |