Addgene

Showing: 41 - 60 of 99166 results

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pOBCol3.6-GFPtpz

Plasmid

#110211

Purpose

Labels immature osteoblasts and connective tissue cells expressing type 1 collagen.

Depositor

David Rowe

Article

Kalajzic et al J Bone Miner Res. 2002 Jan;17(1):15-25.

Insert

eGFPtopaz

Use

Tags

None

Expression

Mammalian

Mutation

None

Promoter

3.6 kb rat type 1 collagen + first intron

Availability

Academic Institutions and Nonprofits only

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Designed VEGF-binding scFv G6des1

Plasmid

#110212

Purpose

Yeast Surface Display of G6des1

Depositor

Sarel Fleishman

Insert

G6des1

Use

Tags

cmyc tag

Expression

Yeast

Mutation

L A43P, L Y49F, L Y55F ,L Q89L, L T94V, L P95T, L…

Promoter

Availability

Academic Institutions and Nonprofits only

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Designed VEGF-binding scFv G6des13

Plasmid

#110213

Purpose

Yeast Surface Display of G6des13

Depositor

Sarel Fleishman

Insert

G6des13

Use

Tags

cmyc tag

Expression

Yeast

Mutation

L A43P, L Q89L, L T94D, L P95T, H Y59H, H Y95F

Promoter

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Flag-SPRTN-wt

Plasmid

#110214

Purpose

Expression in mammalian cells of full-length wild-type SPRTN protein with a Flag-tag at N-terminus.

Depositor

Ivana Marinovic-Terzic, Kristijan Ramadan

Article

Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:

Insert

SPRTN (SPRTN Human)

Use

Tags

Expression

Mammalian

Mutation

P296L (see depositor comments below)

Promoter

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Flag-SPRTN-E112A

Plasmid

#110215

Purpose

Expression in mammalian cells of of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, and Flag-tag at N-terminus.

Depositor

Ivana Marinovic-Terzic, Kristijan Ramadan

Article

Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:

Insert

SPRTN (SPRTN Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

E112A, P296L (see depositor comments below)

Promoter

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Flag-SPRTN-Y117C

Plasmid

#110216

Purpose

Expression in mammalian cells of of full-length SPRTN protein carrying Y117C mutation, a catalytically-impaired mutant, and Flag-tag at N-terminus.

Depositor

Ivana Marinovic-Terzic, Kristijan Ramadan

Article

Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

Insert

SPRTN (SPRTN Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Y117C, P296L (see depositor comments below)

Promoter

Availability

Academic Institutions and Nonprofits only

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pNIC-ZB-SPRTNwt-FL

Plasmid

#110217

Purpose

Expression in E. coli of full-length wild-type SPRTN protein, with His and ZB tags at N-terminus.

Depositor

Kristijan Ramadan

Article

Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

Insert

SPRTN (SPRTN Human)

Use

Tags

Expression

Bacterial

Mutation

P296L (see depositor comments below)

Promoter

Availability

Academic Institutions and Nonprofits only

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pNIC-ZB-SPRTN-E112A-FL

Plasmid

#110218

Purpose

Expression in E. coli of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, with His and ZB tags at N-terminus.

Depositor

Kristijan Ramadan

Article

Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

Insert

SPRTN (SPRTN Human)

Use

Tags

His6 and ZB

Expression

Bacterial

Mutation

E112A, P296L (see depositor comments below)

Promoter

Availability

Academic Institutions and Nonprofits only

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pNIC-ZB-SPRTN-Y117C-FL

Plasmid

#110219

Purpose

Expression in E. coli of full-length SPRTN protein carrying Y112A mutation, a catalytically impaired mutant, with His and ZB tags at N-terminus.

Depositor

Kristijan Ramadan

Article

Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

Insert

SPRTN (SPRTN Human)

Use

Tags

His6 and ZB

Expression

Bacterial

Mutation

Y117C, P296L (see depositor comments below)

Promoter

Availability

Academic Institutions and Nonprofits only

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pAW1

Plasmid

#110220

Purpose

integration of loxP-ura4+-loxM3 cassette

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Insert

ura4+ (ura4 Fission Yeast)

Use

Cre/Lox

Tags

Expression

Mutation

See depositor comments below.

Promoter

Availability

Academic Institutions and Nonprofits only

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pAW5

Plasmid

#110221

Purpose

Cre-expression plasmid

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pAW8

Plasmid

#110222

Purpose

Cre-expression plasmid for cassette exchange

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pAW8_Xho

Plasmid

#110223

Purpose

Cre-expression plasmid for cassette exchange but MCS modified compared to pAW8

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW12

Plasmid

#110224

Purpose

integration of ura4+-loxM3 cassette

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Insert

ura4+ (ura4 Fission Yeast)

Use

Cre/Lox

Tags

Expression

Mutation

See depositor comments below.

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW32

Plasmid

#110225

Purpose

integration of loxP site directly upstream of gene start site - contains S. pombe nmt1 promoter

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW33

Plasmid

#110226

Purpose

integration of loxP site directly upstream of gene start site - contains S. pombe nmt41 promoter

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW34

Plasmid

#110227

Purpose

integration of loxP site directly upstream of gene start site - contains S. pombe nmt81 promoter

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW41

Plasmid

#110228

Purpose

integration of loxP site a distance upstream of gene start site

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Insert

ura4+ (ura4 Fission Yeast)

Use

Cre/Lox

Tags

Expression

Mutation

See depositor comments below.

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW8_yEGFP

Plasmid

#110229

Purpose

Cre/Lox C-terminal tagging construct encoding codon-optimized green fluorescent protein

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW8_yECitrine

Plasmid

#110231

Purpose

Cre/Lox C-terminal tagging construct encoding codon-optimized yellow fluorescent protein

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge

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