Skip to main content
Holiday Schedule: Addgene will be closed November 24th & 25th for the Thanksgiving Holiday. Order processing and shipping may be delayed during this week. For questions about estimated ship dates, please feel free to track your order status or contact [email protected].
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pGP-CMV-GCaMP6s Citations (126)

Plasmid Article: Ultrasensitive fluorescent proteins for imaging neuronal activity.
Chen TW, Wardill TJ, Sun Y, Pulver SR, Renninger SL, Baohan A, Schreiter ER, Kerr RA, Orger MB, Jayaraman V, Looger LL, Svoboda K, Kim DS Nature. 2013 Jul 18;499(7458):295-300. doi: 10.1038/nature12354.
PubMed Journal

Articles Citing pGP-CMV-GCaMP6s

Articles
A polarized Ca(2+), diacylglycerol and STIM1 signalling system regulates directed cell migration. Tsai FC, Seki A, Yang HW, Hayer A, Carrasco S, Malmersjo S, Meyer T. Nat Cell Biol. 2014 Feb;16(2):133-44. doi: 10.1038/ncb2906. Epub 2014 Jan 26. PubMed

Associated Plasmids

Fast gene transfer into the adult zebrafish brain by herpes simplex virus 1 (HSV-1) and electroporation: methods and optogenetic applications. Zou M, De Koninck P, Neve RL, Friedrich RW. Front Neural Circuits. 2014 May 6;8:41. doi: 10.3389/fncir.2014.00041. eCollection 2014. PubMed
Activation of TRPV1 mediates thymic stromal lymphopoietin release via the Ca2+/NFAT pathway in airway epithelial cells. Jia X, Zhang H, Cao X, Yin Y, Zhang B. FEBS Lett. 2014 Aug 25;588(17):3047-54. doi: 10.1016/j.febslet.2014.06.018. Epub 2014 Jun 12. PubMed
Descending control of swim posture by a midbrain nucleus in zebrafish. Thiele TR, Donovan JC, Baier H. Neuron. 2014 Aug 6;83(3):679-91. doi: 10.1016/j.neuron.2014.04.018. Epub 2014 Jul 24. PubMed
Exome sequencing reveals mutation in GJA1 as a cause of keratoderma-hypotrichosis-leukonychia totalis syndrome. Wang H, Cao X, Lin Z, Lee M, Jia X, Ren Y, Dai L, Guan L, Zhang J, Lin X, Zhang J, Chen Q, Feng C, Zhou EY, Yin J, Xu G, Yang Y. Hum Mol Genet. 2015 Jan 1;24(1):243-50. doi: 10.1093/hmg/ddu442. Epub 2014 Aug 28. PubMed
Melatonin signaling controls circadian swimming behavior in marine zooplankton. Tosches MA, Bucher D, Vopalensky P, Arendt D. Cell. 2014 Sep 25;159(1):46-57. doi: 10.1016/j.cell.2014.07.042. PubMed
Simvastatin potently induces calcium-dependent apoptosis of human leiomyoma cells. Borahay MA, Kilic GS, Yallampalli C, Snyder RR, Hankins GD, Al-Hendy A, Boehning D. J Biol Chem. 2014 Dec 19;289(51):35075-86. doi: 10.1074/jbc.M114.583575. Epub 2014 Oct 30. PubMed
Spontaneous Activity Drives Local Synaptic Plasticity In Vivo. Winnubst J, Cheyne JE, Niculescu D, Lohmann C. Neuron. 2015 Jul 15;87(2):399-410. doi: 10.1016/j.neuron.2015.06.029. PubMed
Use of genetically-encoded calcium indicators for live cell calcium imaging and localization in virus-infected cells. Perry JL, Ramachandran NK, Utama B, Hyser JM. Methods. 2015 Nov 15;90:28-38. doi: 10.1016/j.ymeth.2015.09.004. Epub 2015 Sep 3. PubMed
Two-photon fluorescence lifetime imaging of primed SNARE complexes in presynaptic terminals and beta cells. Takahashi N, Sawada W, Noguchi J, Watanabe S, Ucar H, Hayashi-Takagi A, Yagishita S, Ohno M, Tokumaru H, Kasai H. Nat Commun. 2015 Oct 6;6:8531. doi: 10.1038/ncomms9531. PubMed
Reciprocal Regulation of Mitochondrial Dynamics and Calcium Signaling in Astrocyte Processes. Jackson JG, Robinson MB. J Neurosci. 2015 Nov 11;35(45):15199-213. doi: 10.1523/JNEUROSCI.2049-15.2015. PubMed
A comparison of fluorescent Ca(2+) indicators for imaging local Ca(2+) signals in cultured cells. Lock JT, Parker I, Smith IF. Cell Calcium. 2015 Dec;58(6):638-48. doi: 10.1016/j.ceca.2015.10.003. Epub 2015 Oct 29. PubMed
Miro1 Regulates Activity-Driven Positioning of Mitochondria within Astrocytic Processes Apposed to Synapses to Regulate Intracellular Calcium Signaling. Stephen TL, Higgs NF, Sheehan DF, Al Awabdh S, Lopez-Domenech G, Arancibia-Carcamo IL, Kittler JT. J Neurosci. 2015 Dec 2;35(48):15996-6011. doi: 10.1523/JNEUROSCI.2068-15.2015. PubMed
Activation and inhibition of tph2 serotonergic neurons operate in tandem to influence larval zebrafish preference for light over darkness. Cheng RK, Krishnan S, Jesuthasan S. Sci Rep. 2016 Feb 12;6:20788. doi: 10.1038/srep20788. PubMed
In Vivo Killing Capacity of Cytotoxic T Cells Is Limited and Involves Dynamic Interactions and T Cell Cooperativity. Halle S, Keyser KA, Stahl FR, Busche A, Marquardt A, Zheng X, Galla M, Heissmeyer V, Heller K, Boelter J, Wagner K, Bischoff Y, Martens R, Braun A, Werth K, Uvarovskii A, Kempf H, Meyer-Hermann M, Arens R, Kremer M, Sutter G, Messerle M, Forster R. Immunity. 2016 Feb 16;44(2):233-45. doi: 10.1016/j.immuni.2016.01.010. Epub 2016 Feb 9. PubMed
Transient Oxygen/Glucose Deprivation Causes a Delayed Loss of Mitochondria and Increases Spontaneous Calcium Signaling in Astrocytic Processes. O'Donnell JC, Jackson JG, Robinson MB. J Neurosci. 2016 Jul 6;36(27):7109-27. doi: 10.1523/JNEUROSCI.4518-15.2016. PubMed
Unconventional secretory processing diversifies neuronal ion channel properties. Hanus C, Geptin H, Tushev G, Garg S, Alvarez-Castelao B, Sambandan S, Kochen L, Hafner AS, Langer JD, Schuman EM. Elife. 2016 Sep 28;5. pii: e20609. doi: 10.7554/eLife.20609. PubMed
Unstable neurons underlie a stable learned behavior. Liberti WA 3rd, Markowitz JE, Perkins LN, Liberti DC, Leman DP, Guitchounts G, Velho T, Kotton DN, Lois C, Gardner TJ. Nat Neurosci. 2016 Oct 10. doi: 10.1038/nn.4405. PubMed

Associated Plasmids

Generation of transgenic marmosets expressing genetically encoded calcium indicators. Park JE, Zhang XF, Choi SH, Okahara J, Sasaki E, Silva AC. Sci Rep. 2016 Oct 11;6:34931. doi: 10.1038/srep34931. PubMed
L-type calcium channels regulate filopodia stability and cancer cell invasion downstream of integrin signalling. Jacquemet G, Baghirov H, Georgiadou M, Sihto H, Peuhu E, Cettour-Janet P, He T, Perala M, Kronqvist P, Joensuu H, Ivaska J. Nat Commun. 2016 Dec 2;7:13297. doi: 10.1038/ncomms13297. PubMed
Local and Use-Dependent Effects of beta-Amyloid Oligomers on NMDA Receptor Function Revealed by Optical Quantal Analysis. Sinnen BL, Bowen AB, Gibson ES, Kennedy MJ. J Neurosci. 2016 Nov 9;36(45):11532-11543. PubMed
Design and mechanistic insight into ultrafast calcium indicators for monitoring intracellular calcium dynamics. Helassa N, Podor B, Fine A, Torok K. Sci Rep. 2016 Dec 6;6:38276. doi: 10.1038/srep38276. PubMed

Associated Plasmids

Genetically encoded calcium indicators for studying long-term calcium dynamics during apoptosis. Garcia MI, Chen JJ, Boehning D. Cell Calcium. 2017 Jan 3. pii: S0143-4160(16)30167-1. doi: 10.1016/j.ceca.2016.12.010. PubMed
Optogenetic Control of Synaptic Composition and Function. Sinnen BL, Bowen AB, Forte JS, Hiester BG, Crosby KC, Gibson ES, Dell'Acqua ML, Kennedy MJ. Neuron. 2017 Feb 8;93(3):646-660.e5. doi: 10.1016/j.neuron.2016.12.037. Epub 2017 Jan 26. PubMed

Associated Plasmids

Lipid transport by TMEM24 at ER-plasma membrane contacts regulates pulsatile insulin secretion. Lees JA, Messa M, Sun EW, Wheeler H, Torta F, Wenk MR, De Camilli P, Reinisch KM. Science. 2017 Feb 17;355(6326). pii: eaah6171. doi: 10.1126/science.aah6171. PubMed
The schizophrenia- and autism-associated gene, transcription factor 4 regulates the columnar distribution of layer 2/3 prefrontal pyramidal neurons in an activity-dependent manner. Page SC, Hamersky GR, Gallo RA, Rannals MD, Calcaterra NE, Campbell MN, Mayfield B, Briley A, Phan BN, Jaffe AE, Maher BJ. Mol Psychiatry. 2018 Feb;23(2):304-315. doi: 10.1038/mp.2017.37. Epub 2017 Mar 14. PubMed
Astrocyte Ca2+ Influx Negatively Regulates Neuronal Activity. Zhang YV, Ormerod KG, Littleton JT. eNeuro. 2017 Mar 10;4(2). pii: ENEURO.0340-16.2017. doi: 10.1523/ENEURO.0340-16.2017. eCollection 2017 Mar-Apr. PubMed
Heterogeneity of Calcium Responses to Secretagogues in Corticotrophs From Male Rats. Romano N, McClafferty H, Walker JJ, Le Tissier P, Shipston MJ. Endocrinology. 2017 Jun 1;158(6):1849-1858. doi: 10.1210/en.2017-00107. PubMed
THADA Regulates the Organismal Balance between Energy Storage and Heat Production. Moraru A, Cakan-Akdogan G, Strassburger K, Males M, Mueller S, Jabs M, Muelleder M, Frejno M, Braeckman BP, Ralser M, Teleman AA. Dev Cell. 2017 Apr 10;41(1):72-81.e6. doi: 10.1016/j.devcel.2017.03.016. PubMed
Activation of the hypothalamic feeding centre upon visual prey detection. Muto A, Lal P, Ailani D, Abe G, Itoh M, Kawakami K. Nat Commun. 2017 Apr 20;8:15029. doi: 10.1038/ncomms15029. PubMed
The Sec14-like phosphatidylinositol transfer proteins Sec14l3/SEC14L2 act as GTPase proteins to mediate Wnt/Ca2+ signaling. Gong B, Shen W, Xiao W, Meng Y, Meng A, Jia S. Elife. 2017 May 2;6. pii: e26362. doi: 10.7554/eLife.26362. PubMed
Serotonergic Projections Govern Postnatal Neuroblast Migration. Garcia-Gonzalez D, Khodosevich K, Watanabe Y, Rollenhagen A, Lubke JHR, Monyer H. Neuron. 2017 May 3;94(3):534-549.e9. doi: 10.1016/j.neuron.2017.04.013. PubMed
TRPM7 Is Involved in Volume Regulation in Salivary Glands. Kim JM, Choi S, Park K. J Dent Res. 2017 Aug;96(9):1044-1050. doi: 10.1177/0022034517708766. Epub 2017 May 12. PubMed
Thermogenetic neurostimulation with single-cell resolution. Ermakova YG, Lanin AA, Fedotov IV, Roshchin M, Kelmanson IV, Kulik D, Bogdanova YA, Shokhina AG, Bilan DS, Staroverov DB, Balaban PM, Fedotov AB, Sidorov-Biryukov DA, Nikitin ES, Zheltikov AM, Belousov VV. Nat Commun. 2017 May 22;8:15362. doi: 10.1038/ncomms15362. PubMed
A calcium- and light-gated switch to induce gene expression in activated neurons. Lee D, Hyun JH, Jung K, Hannan P, Kwon HB. Nat Biotechnol. 2017 Jun 26. doi: 10.1038/nbt.3902. PubMed

Associated Plasmids

TRPM7 senses oxidative stress to release Zn(2+) from unique intracellular vesicles. Abiria SA, Krapivinsky G, Sah R, Santa-Cruz AG, Chaudhuri D, Zhang J, Adstamongkonkul P, DeCaen PG, Clapham DE. Proc Natl Acad Sci U S A. 2017 Jul 25;114(30):E6079-E6088. doi: 10.1073/pnas.1707380114. Epub 2017 Jul 10. PubMed
Pancreatic Islet Blood Flow Dynamics in Primates. Diez JA, Arrojo E Drigo R, Zheng X, Stelmashenko OV, Chua M, Rodriguez-Diaz R, Fukuda M, Kohler M, Leibiger I, Tun SBB, Ali Y, Augustine GJ, Barathi VA, Berggren PO. Cell Rep. 2017 Aug 8;20(6):1490-1501. doi: 10.1016/j.celrep.2017.07.039. PubMed
Tanycytes control the hormonal output of the hypothalamic-pituitary-thyroid axis. Muller-Fielitz H, Stahr M, Bernau M, Richter M, Abele S, Krajka V, Benzin A, Wenzel J, Kalies K, Mittag J, Heuer H, Offermanns S, Schwaninger M. Nat Commun. 2017 Sep 7;8(1):484. doi: 10.1038/s41467-017-00604-6. PubMed
Visualisation of an nsPEF induced calcium wave using the genetically encoded calcium indicator GCaMP in U87 human glioblastoma cells. Carr L, Bardet SM, Arnaud-Cormos D, Leveque P, O'Connor RP. Bioelectrochemistry. 2018 Feb;119:68-75. doi: 10.1016/j.bioelechem.2017.09.003. Epub 2017 Sep 8. PubMed
Functionally redundant control of cardiac hypertrophic signaling by inositol 1,4,5-trisphosphate receptors. Garcia MI, Karlstaedt A, Chen JJ, Amione-Guerra J, Youker KA, Taegtmeyer H, Boehning D. J Mol Cell Cardiol. 2017 Nov;112:95-103. doi: 10.1016/j.yjmcc.2017.09.006. Epub 2017 Sep 18. PubMed
Different developmental histories of beta-cells generate functional and proliferative heterogeneity during islet growth. Singh SP, Janjuha S, Hartmann T, Kayisoglu O, Konantz J, Birke S, Murawala P, Alfar EA, Murata K, Eugster A, Tsuji N, Morrissey ER, Brand M, Ninov N. Nat Commun. 2017 Sep 22;8(1):664. doi: 10.1038/s41467-017-00461-3. PubMed

Associated Plasmids

MTA promotes chemotaxis and chemokinesis of immune cells through distinct calcium-sensing receptor signaling pathways. Chang F, Kim JM, Choi Y, Park K. Biomaterials. 2018 Jan;150:14-24. doi: 10.1016/j.biomaterials.2017.10.009. Epub 2017 Oct 4. PubMed
Activity-dependent expression of Channelrhodopsin at neuronal synapses. Gobbo F, Marchetti L, Jacob A, Pinto B, Binini N, Pecoraro Bisogni F, Alia C, Luin S, Caleo M, Fellin T, Cancedda L, Cattaneo A. Nat Commun. 2017 Nov 20;8(1):1629. doi: 10.1038/s41467-017-01699-7. PubMed
Neurotransmitter-mediated activity spatially controls neuronal migration in the zebrafish cerebellum. Theisen U, Hennig C, Ring T, Schnabel R, Koster RW. PLoS Biol. 2018 Jan 4;16(1):e2002226. doi: 10.1371/journal.pbio.2002226. eCollection 2018 Jan. PubMed
A dual-ligand-modulable fluorescent protein based on UnaG and calmodulin. Shitashima Y, Shimozawa T, Asahi T, Miyawaki A. Biochem Biophys Res Commun. 2018 Feb 12;496(3):872-879. doi: 10.1016/j.bbrc.2018.01.134. Epub 2018 Feb 1. PubMed
Efficient differentiation of cardiomyocytes and generation of calcium-sensor reporter lines from nonhuman primate iPSCs. Lin Y, Liu H, Klein M, Ostrominski J, Hong SG, Yada RC, Chen G, Navarengom K, Schwartzbeck R, San H, Yu ZX, Liu C, Linask K, Beers J, Qiu L, Dunbar CE, Boehm M, Zou J. Sci Rep. 2018 Apr 12;8(1):5907. doi: 10.1038/s41598-018-24074-y. PubMed
Cortical Circuit Activity Evokes Rapid Astrocyte Calcium Signals on a Similar Timescale to Neurons. Stobart JL, Ferrari KD, Barrett MJP, Gluck C, Stobart MJ, Zuend M, Weber B. Neuron. 2018 May 16;98(4):726-735.e4. doi: 10.1016/j.neuron.2018.03.050. Epub 2018 Apr 26. PubMed
NMDA Receptor Signaling Is Important for Neural Tube Formation and for Preventing Antiepileptic Drug-Induced Neural Tube Defects. Sequerra EB, Goyal R, Castro PA, Levin JB, Borodinsky LN. J Neurosci. 2018 May 16;38(20):4762-4773. doi: 10.1523/JNEUROSCI.2634-17.2018. Epub 2018 Apr 30. PubMed
Filopodia Conduct Target Selection in Cortical Neurons Using Differences in Signal Kinetics of a Single Kinase. Mao YT, Zhu JX, Hanamura K, Iurilli G, Datta SR, Dalva MB. Neuron. 2018 May 16;98(4):767-782.e8. doi: 10.1016/j.neuron.2018.04.011. Epub 2018 May 3. PubMed
Auto-FPFA: An Automated Microscope for Characterizing Genetically Encoded Biosensors. Nguyen TA, Puhl HL 3rd, Pham AK, Vogel SS. Sci Rep. 2018 May 9;8(1):7374. doi: 10.1038/s41598-018-25689-x. PubMed
Activation of airway epithelial bitter taste receptors by Pseudomonas aeruginosa quinolones modulates calcium, cyclic-AMP, and nitric oxide signaling. Freund JR, Mansfield CJ, Doghramji LJ, Adappa ND, Palmer JN, Kennedy DW, Reed DR, Jiang P, Lee RJ. J Biol Chem. 2018 Jun 22;293(25):9824-9840. doi: 10.1074/jbc.RA117.001005. Epub 2018 May 10. PubMed
Top-down descending facilitation of spinal sensory excitatory transmission from the anterior cingulate cortex. Chen T, Taniguchi W, Chen QY, Tozaki-Saitoh H, Song Q, Liu RH, Koga K, Matsuda T, Kaito-Sugimura Y, Wang J, Li ZH, Lu YC, Inoue K, Tsuda M, Li YQ, Nakatsuka T, Zhuo M. Nat Commun. 2018 May 14;9(1):1886. doi: 10.1038/s41467-018-04309-2. PubMed
Voltage-dependent Ca(2+) channels promote branching morphogenesis of salivary glands by patterning differential growth. Kim JM, Choi S, Lee SW, Park K. Sci Rep. 2018 May 15;8(1):7566. doi: 10.1038/s41598-018-25957-w. PubMed
A Critical Neurodevelopmental Role for L-Type Voltage-Gated Calcium Channels in Neurite Extension and Radial Migration. Kamijo S, Ishii Y, Horigane SI, Suzuki K, Ohkura M, Nakai J, Fujii H, Takemoto-Kimura S, Bito H. J Neurosci. 2018 Jun 13;38(24):5551-5566. doi: 10.1523/JNEUROSCI.2357-17.2018. Epub 2018 May 17. PubMed
Activity-Dependent Actin Remodeling at the Base of Dendritic Spines Promotes Microtubule Entry. Schatzle P, Esteves da Silva M, Tas RP, Katrukha EA, Hu HY, Wierenga CJ, Kapitein LC, Hoogenraad CC. Curr Biol. 2018 Jul 9;28(13):2081-2093.e6. doi: 10.1016/j.cub.2018.05.004. Epub 2018 Jun 14. PubMed
OFF-responses of interneurons optimize avoidance behaviors depending on stimulus strength via electrical synapses. Hori S, Oda S, Suehiro Y, Iino Y, Mitani S. PLoS Genet. 2018 Jun 25;14(6):e1007477. doi: 10.1371/journal.pgen.1007477. eCollection 2018 Jun. PubMed

Associated Plasmids

Characterization of developmental and molecular factors underlying release heterogeneity at Drosophila synapses. Akbergenova Y, Cunningham KL, Zhang YV, Weiss S, Littleton JT. Elife. 2018 Jul 10;7. pii: 38268. doi: 10.7554/eLife.38268. PubMed
Cryo-EM structure of the insect olfactory receptor Orco. Butterwick JA, Del Marmol J, Kim KH, Kahlson MA, Rogow JA, Walz T, Ruta V. Nature. 2018 Aug;560(7719):447-452. doi: 10.1038/s41586-018-0420-8. Epub 2018 Aug 15. PubMed
Color opponency with a single kind of bistable opsin in the zebrafish pineal organ. Wada S, Shen B, Kawano-Yamashita E, Nagata T, Hibi M, Tamotsu S, Koyanagi M, Terakita A. Proc Natl Acad Sci U S A. 2018 Oct 30;115(44):11310-11315. doi: 10.1073/pnas.1802592115. Epub 2018 Oct 15. PubMed
Neural circuitry of a polycystin-mediated hydrodynamic startle response for predator avoidance. Bezares-Calderon LA, Berger J, Jasek S, Veraszto C, Mendes S, Guhmann M, Almeda R, Shahidi R, Jekely G. Elife. 2018 Dec 14;7. pii: 36262. doi: 10.7554/eLife.36262. PubMed
GABA and glutamate neurons in the VTA regulate sleep and wakefulness. Yu X, Li W, Ma Y, Tossell K, Harris JJ, Harding EC, Ba W, Miracca G, Wang D, Li L, Guo J, Chen M, Li Y, Yustos R, Vyssotski AL, Burdakov D, Yang Q, Dong H, Franks NP, Wisden W. Nat Neurosci. 2019 Jan;22(1):106-119. doi: 10.1038/s41593-018-0288-9. Epub 2018 Dec 17. PubMed

Associated Plasmids

Calcium oscillations coordinate feather mesenchymal cell movement by SHH dependent modulation of gap junction networks. Li A, Cho JH, Reid B, Tseng CC, He L, Tan P, Yeh CY, Wu P, Li Y, Widelitz RB, Zhou Y, Zhao M, Chow RH, Chuong CM. Nat Commun. 2018 Dec 18;9(1):5377. doi: 10.1038/s41467-018-07661-5. PubMed
Spatially Stable Mitochondrial Compartments Fuel Local Translation during Plasticity. Rangaraju V, Lauterbach M, Schuman EM. Cell. 2019 Jan 10;176(1-2):73-84.e15. doi: 10.1016/j.cell.2018.12.013. Epub 2019 Jan 3. PubMed
Small-Molecule Agonists of Ae. aegypti Neuropeptide Y Receptor Block Mosquito Biting. Duvall LB, Ramos-Espiritu L, Barsoum KE, Glickman JF, Vosshall LB. Cell. 2019 Feb 7;176(4):687-701.e5. doi: 10.1016/j.cell.2018.12.004. PubMed
Analysis of neural activity with fluorescent protein biosensors. Burke RD, Yaguchi S. Methods Cell Biol. 2019;151:519-526. doi: 10.1016/bs.mcb.2018.10.010. Epub 2018 Dec 3. PubMed
Step-function luminopsins for bimodal prolonged neuromodulation. Berglund K, Fernandez AM, Gutekunst CN, Hochgeschwender U, Gross RE. J Neurosci Res. 2019 Apr 7. doi: 10.1002/jnr.24424. PubMed

Associated Plasmids

An Optogenetic Controllable T Cell System for Hepatocellular Carcinoma Immunotherapy. Zhao B, Wang Y, Tan X, Zheng X, Wang F, Ke K, Zhang C, Liao N, Dang Y, Shi Y, Zheng Y, Gao Y, Li Q, Liu X, Liu J. Theranostics. 2019 Mar 6;9(7):1837-1850. doi: 10.7150/thno.27051. eCollection 2019. PubMed
Electronic Cigarette Vapor with Nicotine Causes Airway Mucociliary Dysfunction Preferentially via TRPA1 Receptors. Chung S, Baumlin N, Dennis JS, Moore R, Salathe SF, Whitney PL, Sabater J, Abraham WM, Kim MD, Salathe M. Am J Respir Crit Care Med. 2019 Jun 7. doi: 10.1164/rccm.201811-2087OC. PubMed
Reversible silencing of endogenous receptors in intact brain tissue using 2-photon pharmacology. Pittolo S, Lee H, Llado A, Tosi S, Bosch M, Bardia L, Gomez-Santacana X, Llebaria A, Soriano E, Colombelli J, Poskanzer KE, Perea G, Gorostiza P. Proc Natl Acad Sci U S A. 2019 Jul 2;116(27):13680-13689. doi: 10.1073/pnas.1900430116. Epub 2019 Jun 13. PubMed
Transient Receptor Potential V Channels Are Essential for Glucose Sensing by Aldolase and AMPK. Li M, Zhang CS, Zong Y, Feng JW, Ma T, Hu M, Lin Z, Li X, Xie C, Wu Y, Jiang D, Li Y, Zhang C, Tian X, Wang W, Yang Y, Chen J, Cui J, Wu YQ, Chen X, Liu QF, Wu J, Lin SY, Ye Z, Liu Y, Piao HL, Yu L, Zhou Z, Xie XS, Hardie DG, Lin SC. Cell Metab. 2019 Sep 3;30(3):508-524.e12. doi: 10.1016/j.cmet.2019.05.018. Epub 2019 Jun 13. PubMed

Associated Plasmids

Live calcium imaging of Aedes aegypti neuronal tissues reveals differential importance of chemosensory systems for life-history-specific foraging strategies. Bui M, Shyong J, Lutz EK, Yang T, Li M, Truong K, Arvidson R, Buchman A, Riffell JA, Akbari OS. BMC Neurosci. 2019 Jun 17;20(1):27. doi: 10.1186/s12868-019-0511-y. PubMed
Optochemogenetics Stimulation of Transplanted iPS-NPCs Enhances Neuronal Repair and Functional Recovery after Ischemic Stroke. Ping Yu S, Tung JK, Wei ZZ, Chen D, Berglund K, Zhong W, Zhang JY, Gu X, Song M, Gross RE, Lin SZ, Wei L. J Neurosci. 2019 Jul 1. pii: JNEUROSCI.2010-18.2019. doi: 10.1523/JNEUROSCI.2010-18.2019. PubMed
Rotavirus Calcium Dysregulation Manifests as Dynamic Calcium Signaling in the Cytoplasm and Endoplasmic Reticulum. Chang-Graham AL, Perry JL, Strtak AC, Ramachandran NK, Criglar JM, Philip AA, Patton JT, Estes MK, Hyser JM. Sci Rep. 2019 Jul 25;9(1):10822. doi: 10.1038/s41598-019-46856-8. PubMed

Associated Plasmids

Phosphoproteomics reveals conserved exercise-stimulated signaling and AMPK regulation of store-operated calcium entry. Nelson ME, Parker BL, Burchfield JG, Hoffman NJ, Needham EJ, Cooke KC, Naim T, Sylow L, Ling NX, Francis D, Norris DM, Chaudhuri R, Oakhill JS, Richter EA, Lynch GS, Stockli J, James DE. EMBO J. 2019 Dec 16;38(24):e102578. doi: 10.15252/embj.2019102578. Epub 2019 Aug 5. PubMed
Remotely controlled chemomagnetic modulation of targeted neural circuits. Rao S, Chen R, LaRocca AA, Christiansen MG, Senko AW, Shi CH, Chiang PH, Varnavides G, Xue J, Zhou Y, Park S, Ding R, Moon J, Feng G, Anikeeva P. Nat Nanotechnol. 2019 Oct;14(10):967-973. doi: 10.1038/s41565-019-0521-z. Epub 2019 Aug 19. PubMed
Design and development of stapled transmembrane peptides that disrupt the activity of G-protein-coupled receptor oligomers. Botta J, Bibic L, Killoran P, McCormick PJ, Howell LA. J Biol Chem. 2019 Nov 8;294(45):16587-16603. doi: 10.1074/jbc.RA119.009160. Epub 2019 Aug 29. PubMed
High-throughput-compatible assays using a genetically-encoded calcium indicator. Wu N, Nishioka WK, Derecki NC, Maher MP. Sci Rep. 2019 Sep 3;9(1):12692. doi: 10.1038/s41598-019-49070-8. PubMed
Oligodendrocytes express synaptic proteins that modulate myelin sheath formation. Hughes AN, Appel B. Nat Commun. 2019 Sep 11;10(1):4125. doi: 10.1038/s41467-019-12059-y. PubMed
Shank Proteins Couple the Endocytic Zone to the Postsynaptic Density to Control Trafficking and Signaling of Metabotropic Glutamate Receptor 5. Scheefhals N, Catsburg LAE, Westerveld ML, Blanpied TA, Hoogenraad CC, MacGillavry HD. Cell Rep. 2019 Oct 8;29(2):258-269.e8. doi: 10.1016/j.celrep.2019.08.102. PubMed
Tau Misfolding Efficiently Propagates between Individual Intact Hippocampal Neurons. Hallinan GI, Vargas-Caballero M, West J, Deinhardt K. J Neurosci. 2019 Nov 27;39(48):9623-9632. doi: 10.1523/JNEUROSCI.1590-19.2019. Epub 2019 Oct 28. PubMed
A centrosome-localized calcium signal is essential for mammalian cell mitosis. Helassa N, Nugues C, Rajamanoharan D, Burgoyne RD, Haynes LP. FASEB J. 2019 Dec;33(12):14602-14610. doi: 10.1096/fj.201901662R. Epub 2019 Nov 2. PubMed
Genetic Indicators for Calcium Signaling Studies in Toxoplasma gondii. Vella SA, Calixto A, Asady B, Li ZH, Moreno SNJ. Methods Mol Biol. 2020;2071:187-207. doi: 10.1007/978-1-4939-9857-9_11. PubMed
Ca2+ transients in melanocyte dendrites and dendritic spine-like structures evoked by cell-to-cell signaling. Belote RL, Simon SM. J Cell Biol. 2020 Jan 6;219(1). pii: 132739. doi: 10.1083/jcb.201902014. PubMed
A Dual Reporter EndoC-betaH1 Human beta-Cell Line for Efficient Quantification of Calcium Flux and Insulin Secretion. Cardenas-Diaz FL, Leavens KF, Kishore S, Osorio-Quintero C, Chen YJ, Stanger BZ, Wang P, French D, Gadue P. Endocrinology. 2020 Feb 1;161(2). pii: 5710816. doi: 10.1210/endocr/bqaa005. PubMed
The Effects of the Anti-aging Protein Klotho on Mucociliary Clearance. Garth J, Easter M, Skylar Harris E, Sailland J, Kuenzi L, Chung S, Dennis JS, Baumlin N, Adewale AT, Rowe SM, King G, Faul C, Barnes JW, Salathe M, Krick S. Front Med (Lausanne). 2020 Jan 24;6:339. doi: 10.3389/fmed.2019.00339. eCollection 2019. PubMed
Biolistic transfection and expression analysis of acute cortical slices. Hamad MIK, Daoud S, Petrova P, Rabaya O, Jbara A, Melliti N, Stichmann S, Reiss G, Herz J, Forster E. J Neurosci Methods. 2020 May 1;337:108666. doi: 10.1016/j.jneumeth.2020.108666. Epub 2020 Feb 28. PubMed
Agonist-mediated switching of ion selectivity in TPC2 differentially promotes lysosomal function. Gerndt S, Chen CC, Chao YK, Yuan Y, Burgstaller S, Scotto Rosato A, Krogsaeter E, Urban N, Jacob K, Nguyen ONP, Miller MT, Keller M, Vollmar AM, Gudermann T, Zierler S, Schredelseker J, Schaefer M, Biel M, Malli R, Wahl-Schott C, Bracher F, Patel S, Grimm C. Elife. 2020 Mar 16;9. pii: 54712. doi: 10.7554/eLife.54712. PubMed
Non-apoptotic cell death induced by opening the large conductance mechanosensitive channel MscL in hepatocellular carcinoma HepG2 cells. Wen X, Tang S, Hong F, Wang X, Chen S, Hong L, Ye J, Li X, Shang S, Jiang M, Mao J, Shu Q, Li Y. Biomaterials. 2020 Aug;250:120061. doi: 10.1016/j.biomaterials.2020.120061. Epub 2020 Apr 25. PubMed
Procedures for Culturing and Genetically Manipulating Murine Hippocampal Postnatal Neurons. Moutin E, Hemonnot AL, Seube V, Linck N, Rassendren F, Perroy J, Compan V. Front Synaptic Neurosci. 2020 Apr 30;12:19. doi: 10.3389/fnsyn.2020.00019. eCollection 2020. PubMed
Rapid 3D Enhanced Resolution Microscopy Reveals Diversity in Dendritic Spinule Dynamics, Regulation, and Function. Zaccard CR, Shapiro L, Martin-de-Saavedra MD, Pratt C, Myczek K, Song A, Forrest MP, Penzes P. Neuron. 2020 Aug 5;107(3):522-537.e6. doi: 10.1016/j.neuron.2020.04.025. Epub 2020 May 27. PubMed
Development of an L-type Ca(2+) channel-dependent Ca(2+) transient during the radial migration of cortical excitatory neurons. Horigane SI, Hamada S, Kamijo S, Yamada H, Yamasaki M, Watanabe M, Bito H, Ohtsuka T, Takemoto-Kimura S. Neurosci Res. 2020 Jun 26. pii: S0168-0102(20)30391-6. doi: 10.1016/j.neures.2020.06.003. PubMed
In situ electrochemical generation of nitric oxide for neuronal modulation. Park J, Jin K, Sahasrabudhe A, Chiang PH, Maalouf JH, Koehler F, Rosenfeld D, Rao S, Tanaka T, Khudiyev T, Schiffer ZJ, Fink Y, Yizhar O, Manthiram K, Anikeeva P. Nat Nanotechnol. 2020 Aug;15(8):690-697. doi: 10.1038/s41565-020-0701-x. Epub 2020 Jun 29. PubMed
Calcium spikes accompany cleavage furrow ingression and cell separation during fission yeast cytokinesis. Poddar A, Sidibe O, Ray A, Chen Q. Mol Biol Cell. 2021 Jan 1;32(1):15-27. doi: 10.1091/mbc.E20-09-0609. Epub 2020 Nov 11. PubMed
Neuronal Activity at Synapse Resolution: Reporters and Effectors for Synaptic Neuroscience. Gobbo F, Cattaneo A. Front Mol Neurosci. 2020 Oct 21;13:572312. doi: 10.3389/fnmol.2020.572312. eCollection 2020. PubMed
P2X-GCaMPs as Versatile Tools for Imaging Extracellular ATP Signaling. Ollivier M, Beudez J, Linck N, Grutter T, Compan V, Rassendren F. eNeuro. 2021 Jan 28;8(1). pii: ENEURO.0185-20.2020. doi: 10.1523/ENEURO.0185-20.2020. Print 2021 Jan-Feb. PubMed
STIM-Orai1 signaling regulates fluidity of cytoplasm during membrane blebbing. Aoki K, Harada S, Kawaji K, Matsuzawa K, Uchida S, Ikenouchi J. Nat Commun. 2021 Jan 20;12(1):480. doi: 10.1038/s41467-020-20826-5. PubMed
Pluripotency state regulates cytoneme selectivity and self-organization of embryonic stem cells. Junyent S, Reeves J, Gentleman E, Habib SJ. J Cell Biol. 2021 Apr 5;220(4). pii: 211816. doi: 10.1083/jcb.202005095. PubMed
Timescale of hole closure during plasma membrane repair estimated by calcium imaging and numerical modeling. Klenow MB, Heitmann ASB, Nylandsted J, Simonsen AC. Sci Rep. 2021 Feb 19;11(1):4226. doi: 10.1038/s41598-021-82926-6. PubMed
A modular tool to query and inducibly disrupt biomolecular condensates. Hernandez-Candia CN, Pearce S, Tucker CL. Nat Commun. 2021 Mar 22;12(1):1809. doi: 10.1038/s41467-021-22096-1. PubMed
Functional characterization of N-octyl 4-methylamphetamine variants and related bivalent compounds at the dopamine and serotonin transporters using Ca(2+) channels as sensors. Ruchala I, Battisti UM, Nguyen VT, Chen RY, Glennon RA, Eltit JM. Toxicol Appl Pharmacol. 2021 May 15;419:115513. doi: 10.1016/j.taap.2021.115513. Epub 2021 Mar 27. PubMed
Drugs that inhibit TMEM16 proteins block SARS-CoV-2 spike-induced syncytia. Braga L, Ali H, Secco I, Chiavacci E, Neves G, Goldhill D, Penn R, Jimenez-Guardeno JM, Ortega-Prieto AM, Bussani R, Cannata A, Rizzari G, Collesi C, Schneider E, Arosio D, Shah AM, Barclay WS, Malim MH, Burrone J, Giacca M. Nature. 2021 Jun;594(7861):88-93. doi: 10.1038/s41586-021-03491-6. Epub 2021 Apr 7. PubMed
Damage-Induced Calcium Signaling and Reactive Oxygen Species Mediate Macrophage Activation in Zebrafish. Sipka T, Peroceschi R, Hassan-Abdi R, Gross M, Ellett F, Begon-Pescia C, Gonzalez C, Lutfalla G, Nguyen-Chi M. Front Immunol. 2021 Mar 26;12:636585. doi: 10.3389/fimmu.2021.636585. eCollection 2021. PubMed
Protocol for live enhanced resolution confocal imaging of dendritic spinule dynamics in primary mouse cortical neuron culture. Zaccard CR, Kirchenbuechler D, Yoon S, Arvanitis C, Penzes P. STAR Protoc. 2021 Apr 5;2(2):100427. doi: 10.1016/j.xpro.2021.100427. eCollection 2021 Jun 18. PubMed
SARS-COV-2 induced Diarrhea is inflammatory, Ca (2+) Dependent and involves activation of calcium activated Cl channels. Donowitz M, Tse CM, Dokladny K, Rawat M, Horwitz I, Ye C, Kell A, Lin R, Lee S, Guo C, Tsai SJ, Cox A, Gould S, In J, Bradfute S, Zachos NC, Kovbasnjuk O. bioRxiv. 2021 Apr 28. doi: 10.1101/2021.04.27.441695. PubMed
Intravital imaging identifies the VEGF-TXA2 axis as a critical promoter of PGE2 secretion from tumor cells and immune evasion. Konishi Y, Ichise H, Watabe T, Oki C, Tsukiji S, Hamazaki Y, Murakawa Y, Takaori-Kondo A, Terai K, Matsuda M. Cancer Res. 2021 May 25. pii: 0008-5472.CAN-20-4245. doi: 10.1158/0008-5472.CAN-20-4245. PubMed

Associated Plasmids

Live imaging of adult neural stem cells in freely behaving mice using mini-endoscopes. Malvaut S, Marymonchyk A, Gengatharan A, Saghatelyan A. STAR Protoc. 2021 Jun 12;2(2):100596. doi: 10.1016/j.xpro.2021.100596. eCollection 2021 Jun 18. PubMed
Exendin-4 stimulates autophagy in pancreatic beta-cells via the RAPGEF/EPAC-Ca(2+)-PPP3/calcineurin-TFEB axis. Zummo FP, Krishnanda SI, Georgiou M, O'Harte FP, Parthsarathy V, Cullen KS, Honkanen-Scott M, Shaw JA, Lovat PE, Arden C. Autophagy. 2022 Apr;18(4):799-815. doi: 10.1080/15548627.2021.1956123. Epub 2021 Aug 2. PubMed
Rpsa Signaling Regulates Cortical Neuronal Morphogenesis via Its Ligand, PEDF, and Plasma Membrane Interaction Partner, Itga6. Blazejewski SM, Bennison SA, Ha NT, Liu X, Smith TH, Dougherty KJ, Toyo-Oka K. Cereb Cortex. 2021 Aug 4. pii: 6338536. doi: 10.1093/cercor/bhab242. PubMed
A subthreshold synaptic mechanism regulating BDNF expression and resting synaptic strength. Horvath PM, Chanaday NL, Alten B, Kavalali ET, Monteggia LM. Cell Rep. 2021 Aug 3;36(5):109467. doi: 10.1016/j.celrep.2021.109467. PubMed
The structural basis of odorant recognition in insect olfactory receptors. Del Marmol J, Yedlin MA, Ruta V. Nature. 2021 Sep;597(7874):126-131. doi: 10.1038/s41586-021-03794-8. Epub 2021 Aug 4. PubMed
Differential signalling induced by alpha7 nicotinic acetylcholine receptors in hippocampal dentate gyrus in vitro and in vivo. Cheng Q, Lamb P, Stevanovic K, Bernstein BJ, Fry SA, Cushman JD, Yakel JL. J Physiol. 2021 Oct;599(20):4687-4704. doi: 10.1113/JP280505. Epub 2021 Sep 28. PubMed
Activity-dependent regulation of mitochondrial motility in developing cortical dendrites. Silva CA, Yalnizyan-Carson A, Fernandez Busch MV, van Zwieten M, Verhage M, Lohmann C. Elife. 2021 Sep 7;10. pii: 62091. doi: 10.7554/eLife.62091. PubMed
The prevalence and specificity of local protein synthesis during neuronal synaptic plasticity. Sun C, Nold A, Fusco CM, Rangaraju V, Tchumatchenko T, Heilemann M, Schuman EM. Sci Adv. 2021 Sep 17;7(38):eabj0790. doi: 10.1126/sciadv.abj0790. Epub 2021 Sep 17. PubMed
Assessing the Wnt-reactivity of cytonemes of mouse embryonic stem cells using a bioengineering approach. Junyent S, Reeves J, Habib SJ. STAR Protoc. 2021 Sep 15;2(3):100813. doi: 10.1016/j.xpro.2021.100813. eCollection 2021 Sep 17. PubMed
Neurodegenerative processes accelerated by protein malnutrition and decelerated by essential amino acids in a tauopathy mouse model. Sato H, Takado Y, Toyoda S, Tsukamoto-Yasui M, Minatohara K, Takuwa H, Urushihata T, Takahashi M, Shimojo M, Ono M, Maeda J, Orihara A, Sahara N, Aoki I, Karakawa S, Isokawa M, Kawasaki N, Kawasaki M, Ueno S, Kanda M, Nishimura M, Suzuki K, Mitsui A, Nagao K, Kitamura A, Higuchi M. Sci Adv. 2021 Oct 22;7(43):eabd5046. doi: 10.1126/sciadv.abd5046. Epub 2021 Oct 22. PubMed
Nitric Oxide Synthase Neurons in the Preoptic Hypothalamus Are NREM and REM Sleep-Active and Lower Body Temperature. Harding EC, Ba W, Zahir R, Yu X, Yustos R, Hsieh B, Lignos L, Vyssotski AL, Merkle FT, Constandinou TG, Franks NP, Wisden W. Front Neurosci. 2021 Oct 14;15:709825. doi: 10.3389/fnins.2021.709825. eCollection 2021. PubMed
Biophysical and functional characterization of the human TAS1R2 sweet taste receptor overexpressed in a HEK293S inducible cell line. Belloir C, Brule M, Tornier L, Neiers F, Briand L. Sci Rep. 2021 Nov 15;11(1):22238. doi: 10.1038/s41598-021-01731-3. PubMed
Deciphering cell signaling networks with massively multiplexed biosensor barcoding. Yang JM, Chi WY, Liang J, Takayanagi S, Iglesias PA, Huang CH. Cell. 2021 Dec 9;184(25):6193-6206.e14. doi: 10.1016/j.cell.2021.11.005. Epub 2021 Nov 26. PubMed

Associated Plasmids

Calcium-dependent ESCRT recruitment and lysosome exocytosis maintain epithelial integrity during Candida albicans invasion. Westman J, Plumb J, Licht A, Yang M, Allert S, Naglik JR, Hube B, Grinstein S, Maxson ME. Cell Rep. 2022 Jan 4;38(1):110187. doi: 10.1016/j.celrep.2021.110187. PubMed
The transcription factor unc-130/FOXD3/4 contributes to the biphasic calcium response required to optimize avoidance behavior. Hori S, Mitani S. Sci Rep. 2022 Feb 3;12(1):1907. doi: 10.1038/s41598-022-05942-0. PubMed
Absolute measurement of cellular activities using photochromic single-fluorophore biosensors and intermittent quantification. Bierbuesse F, Bourges AC, Gielen V, Monkemoller V, Vandenberg W, Shen Y, Hofkens J, Berghe PV, Campbell RE, Moeyaert B, Dedecker P. Nat Commun. 2022 Apr 6;13(1):1850. doi: 10.1038/s41467-022-29508-w. PubMed
Live-Cell Imaging of the Contractile Velocity and Transient Intracellular Ca(2+) Fluctuations in Human Stem Cell-Derived Cardiomyocytes. Acharya A, Nemade H, Rajendra Prasad K, Khan K, Hescheler J, Blackburn N, Hemmersbach R, Papadopoulos S, Sachinidis A. Cells. 2022 Apr 9;11(8). pii: cells11081280. doi: 10.3390/cells11081280. PubMed
A Bioengineered In Vitro Model to Assess AAV-Based Gene Therapies for Cyclic GMP-Related Disorders. Pavlou M, Babutzka S, Michalakis S. Int J Mol Sci. 2022 Apr 20;23(9). pii: ijms23094538. doi: 10.3390/ijms23094538. PubMed
RNA supply drives physiological granule assembly in neurons. Bauer KE, Bargenda N, Schieweck R, Illig C, Segura I, Harner M, Kiebler MA. Nat Commun. 2022 May 19;13(1):2781. doi: 10.1038/s41467-022-30067-3. PubMed
Peripheral Nerve Injury Induces Changes in the Activity of Inhibitory Interneurons as Visualized in Transgenic GAD1-GCaMP6s Rats. Krishnan V, Wade-Kleyn LC, Israeli RR, Pelled G. Biosensors (Basel). 2022 Jun 1;12(6). pii: bios12060383. doi: 10.3390/bios12060383. PubMed
A Non-Canonical Role for IRE1alpha Links ER and Mitochondria as Key Regulators of Astrocyte Dysfunction: Implications in Methamphetamine use and HIV-Associated Neurocognitive Disorders. Proulx J, Stacy S, Park IW, Borgmann K. Front Neurosci. 2022 Jun 17;16:906651. doi: 10.3389/fnins.2022.906651. eCollection 2022. PubMed

If you have published an article using this plasmid, please email us at [email protected] to have your article added to this page.