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pcDNA3 APEX2-NES Citations (29)

Plasmid Article: Directed evolution of APEX2 for electron microscopy and proximity labeling.
Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, Ting AY Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179.
PubMed Journal

Articles Citing pcDNA3 APEX2-NES

Phosphorylation of NS5A Serine-235 is essential to hepatitis C virus RNA replication and normal replication compartment formation. Eyre NS, Hampton-Smith RJ, Aloia AL, Eddes JS, Simpson KJ, Hoffmann P, Beard MR. Virology. 2016 Apr;491:27-44. doi: 10.1016/j.virol.2016.01.018. Epub 2016 Feb 10. PubMed
Proximity-dependent biotin labeling in yeast using the engineered ascorbate peroxidase APEX2. Hwang J, Espenshade PJ. Biochem J. 2016 Jun 7. pii: BCJ20160106. PubMed

Associated Plasmids

Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens. Marceau CD, Puschnik AS, Majzoub K, Ooi YS, Brewer SM, Fuchs G, Swaminathan K, Mata MA, Elias JE, Sarnow P, Carette JE. Nature. 2016 Jul 7;535(7610):159-63. doi: 10.1038/nature18631. Epub 2016 Jun 17. PubMed
Static Clathrin Assemblies at the Peripheral Vacuole-Plasma Membrane Interface of the Parasitic Protozoan Giardia lamblia. Zumthor JP, Cernikova L, Rout S, Kaech A, Faso C, Hehl AB. PLoS Pathog. 2016 Jul 20;12(7):e1005756. doi: 10.1371/journal.ppat.1005756. eCollection 2016 Jul. PubMed
A Chemically Programmed Proximal Ligand Enhances the Catalytic Properties of a Heme Enzyme. Green AP, Hayashi T, Mittl PR, Hilvert D. J Am Chem Soc. 2016 Sep 7;138(35):11344-52. doi: 10.1021/jacs.6b07029. Epub 2016 Aug 26. PubMed
Development of a Proximity Labeling System to Map the Chlamydia trachomatis Inclusion Membrane. Rucks EA, Olson MG, Jorgenson LM, Srinivasan RR, Ouellette SP. Front Cell Infect Microbiol. 2017 Feb 15;7:40. doi: 10.3389/fcimb.2017.00040. eCollection 2017. PubMed
Multidimensional Tracking of GPCR Signaling via Peroxidase-Catalyzed Proximity Labeling. Paek J, Kalocsay M, Staus DP, Wingler L, Pascolutti R, Paulo JA, Gygi SP, Kruse AC. Cell. 2017 Apr 6;169(2):338-349.e11. doi: 10.1016/j.cell.2017.03.028. PubMed

Associated Plasmids

An Approach to Spatiotemporally Resolve Protein Interaction Networks in Living Cells. Lobingier BT, Huttenhain R, Eichel K, Miller KB, Ting AY, von Zastrow M, Krogan NJ. Cell. 2017 Apr 6;169(2):350-360.e12. doi: 10.1016/j.cell.2017.03.022. PubMed
The ciliary membrane-associated proteome reveals actin-binding proteins as key components of cilia. Kohli P, Hohne M, Jungst C, Bertsch S, Ebert LK, Schauss AC, Benzing T, Rinschen MM, Schermer B. EMBO Rep. 2017 Sep;18(9):1521-1535. doi: 10.15252/embr.201643846. Epub 2017 Jul 14. PubMed
Electron microscopy using the genetically encoded APEX2 tag in cultured mammalian cells. Martell JD, Deerinck TJ, Lam SS, Ellisman MH, Ting AY. Nat Protoc. 2017 Sep;12(9):1792-1816. doi: 10.1038/nprot.2017.065. Epub 2017 Aug 10. PubMed
Production and evaluation of parathyroid hormone receptor1 ligands with intrinsic or assembled peroxidase domains. Charest-Morin X, Poubelle PE, Marceau F. Sci Rep. 2017 Oct 12;7(1):13099. doi: 10.1038/s41598-017-13548-0. PubMed
Bifunctional fusion proteins containing the sequence of the bradykinin homologue maximakinin: activities at the rat bradykinin B2 receptor. Charest-Morin X, Lodge R, Marceau F. Can J Physiol Pharmacol. 2018 May;96(5):459-470. doi: 10.1139/cjpp-2017-0692. Epub 2018 Feb 7. PubMed
Galectins Control mTOR in Response to Endomembrane Damage. Jia J, Abudu YP, Claude-Taupin A, Gu Y, Kumar S, Choi SW, Peters R, Mudd MH, Allers L, Salemi M, Phinney B, Johansen T, Deretic V. Mol Cell. 2018 Apr 5;70(1):120-135.e8. doi: 10.1016/j.molcel.2018.03.009. PubMed

Associated Plasmids

C-BERST: defining subnuclear proteomic landscapes at genomic elements with dCas9-APEX2. Gao XD, Tu LC, Mir A, Rodriguez T, Ding Y, Leszyk J, Dekker J, Shaffer SA, Zhu LJ, Wolfe SA, Sontheimer EJ. Nat Methods. 2018 Jun;15(6):433-436. doi: 10.1038/s41592-018-0006-2. Epub 2018 May 7. PubMed
High-quality ultrastructural preservation using cryofixation for 3D electron microscopy of genetically labeled tissues. Tsang TK, Bushong EA, Boassa D, Hu J, Romoli B, Phan S, Dulcis D, Su CY, Ellisman MH. Elife. 2018 May 11;7. pii: 35524. doi: 10.7554/eLife.35524. PubMed
Proximity Labeling by a Recombinant APEX2-FGF1 Fusion Protein Reveals Interaction of FGF1 with the Proteoglycans CD44 and CSPG4. Zhen Y, Haugsten EM, Singh SK, Wesche J. Biochemistry. 2018 Jul 3;57(26):3807-3816. doi: 10.1021/acs.biochem.8b00120. Epub 2018 Jun 13. PubMed
Meloidogyne incognita PASSE-MURAILLE (MiPM) Gene Encodes a Cell-Penetrating Protein That Interacts With the CSN5 Subunit of the COP9 Signalosome. Bournaud C, Gillet FX, Murad AM, Bresso E, Albuquerque EVS, Grossi-de-Sa MF. Front Plant Sci. 2018 Jun 26;9:904. doi: 10.3389/fpls.2018.00904. eCollection 2018. PubMed
Correlated Light-Serial Scanning Electron Microscopy (CoLSSEM) for ultrastructural visualization of single neurons in vivo. Hirabayashi Y, Tapia JC, Polleux F. Sci Rep. 2018 Sep 27;8(1):14491. doi: 10.1038/s41598-018-32820-5. PubMed
High-resolution mapping of centromeric protein association using APEX-chromatin fibers. Kyriacou E, Heun P. Epigenetics Chromatin. 2018 Nov 16;11(1):68. doi: 10.1186/s13072-018-0237-6. PubMed
Proximity-CLIP provides a snapshot of protein-occupied RNA elements in subcellular compartments. Benhalevy D, Anastasakis DG, Hafner M. Nat Methods. 2018 Dec;15(12):1074-1082. doi: 10.1038/s41592-018-0220-y. Epub 2018 Nov 26. PubMed

Associated Plasmids

Mapping the Proteome of the Synaptic Cleft through Proximity Labeling Reveals New Cleft Proteins. Cijsouw T, Ramsey AM, Lam TT, Carbone BE, Blanpied TA, Biederer T. Proteomes. 2018 Nov 28;6(4). pii: proteomes6040048. doi: 10.3390/proteomes6040048. PubMed
Creation of versatile cloning platforms for transgene expression and dCas9-based epigenome editing. Haldeman JM, Conway AE, Arlotto ME, Slentz DH, Muoio DM, Becker TC, Newgard CB. Nucleic Acids Res. 2018 Dec 27. pii: 5264291. doi: 10.1093/nar/gky1286. PubMed

Associated Plasmids

Misfolded GPI-anchored proteins are escorted through the secretory pathway by ER-derived factors. Zavodszky E, Hegde RS. Elife. 2019 May 16;8. pii: 46740. doi: 10.7554/eLife.46740. PubMed
Proximity labeling reveals novel interactomes in live Drosophila tissue. Mannix KM, Starble RM, Kaufman RS, Cooley L. Development. 2019 Jul 18;146(14). pii: dev.176644. doi: 10.1242/dev.176644. PubMed
Parcas is the predominant Rab11-GEF for rhodopsin transport in Drosophila photoreceptors. Otsuka Y, Satoh T, Nakayama N, Inaba R, Yamashita H, Satoh AK. J Cell Sci. 2019 Aug 7;132(15). pii: jcs.231431. doi: 10.1242/jcs.231431. PubMed
The cysteine-free single mutant C32S of APEX2 is a highly expressed and active fusion tag for proximity labeling applications. Huang MS, Lin WC, Chang JH, Cheng CH, Wang HY, Mou KY. Protein Sci. 2019 Sep;28(9):1703-1712. doi: 10.1002/pro.3685. Epub 2019 Aug 6. PubMed
Proximity Labeling of the Chlamydia trachomatis Inclusion Membrane. Olson MG, Jorgenson LM, Widner RE, Rucks EA. Methods Mol Biol. 2019;2042:245-278. doi: 10.1007/978-1-4939-9694-0_17. PubMed
Proximity Labeling To Map Host-Pathogen Interactions at the Membrane of a Bacterium-Containing Vacuole in Chlamydia trachomatis-Infected Human Cells. Olson MG, Widner RE, Jorgenson LM, Lawrence A, Lagundzin D, Woods NT, Ouellette SP, Rucks EA. Infect Immun. 2019 Oct 18;87(11). pii: IAI.00537-19. doi: 10.1128/IAI.00537-19. Print 2019 Nov. PubMed
Plasticity of the Cullin-RING Ligase Repertoire Shapes Sensitivity to Ligand-Induced Protein Degradation. Mayor-Ruiz C, Jaeger MG, Bauer S, Brand M, Sin C, Hanzl A, Mueller AC, Menche J, Winter GE. Mol Cell. 2019 Aug 22;75(4):849-858.e8. doi: 10.1016/j.molcel.2019.07.013. PubMed

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