3xflag-APEX2-LRRK2
(Plasmid #164622)

• Purpose: Expresses LRRK2 tagged with APEX2 and 3xFLAG
• Depositing Lab: Mark Cookson
• Publication: Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/eabb2454. doi: 10.1126/sciadv.abb2454. Print 2020 Nov.

Backbone

• Vector backbone: CMV-3xFLAG-APEX2-pDEST
• Backbone size w/o insert (bp): 7180
• Total vector size (bp): 13136
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LRRK2
• Alt name: Leucine-rich repeat serine/threonine-protein kinase 2
• Alt name: PARK8
• Alt name: Dardarin
• Species: H. sapiens (human)
• Insert Size (bp): 7584
• GenBank ID: NM_198578.4 NM_198578.4
• Promoter: CMV
• Tags / Fusion Proteins:
  • APEX2 (N terminal on insert)
  • 3xFLAG (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: ATGGCTAGTGGCAGCTGTCAGGGGTGCGAAGAG
• 3′ sequencing primer: TTACTCAACAGATGTTCGTCTCATTTTTTCAGCTAATTCTTTTCTCACTTCAATGTGTT

Resource Information

• Supplemental Documents:
  • 3xFLAG7.1-APEX2-LRRK2.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry