pCS2 LRP6-APEX2-Ires-eGFP-PAC
(Plasmid
#180142)
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Purposeused to biotinylate targets that are recruited near the receptor during Wnt signaling at different time periods
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Depositing Lab
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Depositing OrganizationUniversity of California, Los Angeles (UCLA)
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 180142 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 180142-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 180142-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCS2
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Backbone manufacturerpromega
- Backbone size w/o insert (bp) 4092
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHuman LRP6
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SpeciesH. sapiens (human)
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Entrez GeneLRP6 (a.k.a. ADCAD2, EVR8, STHAG7)
- Promoter SP6
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer SP6
- 3′ sequencing primer T3
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 180142-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 180142-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2 LRP6-APEX2-Ires-eGFP-PAC was a gift from Edward De Robertis (Addgene plasmid # 180142 ; http://n2t.net/addgene:180142 ; RRID:Addgene_180142) -
For your References section:
Wnt-inducible Lrp6-APEX2 interacting proteins identify ESCRT machinery and Trk-fused gene as components of the Wnt signaling pathway. Colozza G, Jami-Alahmadi Y, Dsouza A, Tejeda-Munoz N, Albrecht LV, Sosa EA, Wohlschlegel JA, De Robertis EM. Sci Rep. 2020 Dec 9;10(1):21555. doi: 10.1038/s41598-020-78019-5. 10.1038/s41598-020-78019-5 PubMed 33299006
