169,086 results

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XLone-GFP

Plasmid

#96930

Purpose

Tunable and temporal expression control of GFP

Depositor

Xiaojun Lian

Article

Randolph et al Sci Rep. 2017 May 8;7(1):1549. doi: 10.1

Insert

Green Fluorescent Protein

Expression

Mammalian

Promoter

TRE3GS

Available Since

Aug. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pEFIRES-P-EGFP-ADRP

Plasmid

#87157

Purpose

Fluorescent protein localized to lipid droplets (LDs)

Depositor

Elina Ikonen

Article

Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

Insert

ADRP (PLIN2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

mCherry-R TTGGTCACCTTCAGCTTGG

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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evoBE4-SpRY

Plasmid

#226854

Purpose

Expresses evoBE4max-SpRY base editor in mammalian cells with eGFP marker

Depositor

Alexis Komor

Article

Osgood et al Methods Enzymol. 2025;712:351-404. doi:

Insert

evoBE4max-SpRY

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pBFC0619

Plasmid

#186690

Purpose

VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargo

Depositor

Jennifer Doudna

Article

Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

Inserts

tnsA
tnsB
tnsC
tnsD
tniQ
cas8-cas5 fusion
cas7
cas6
Vc_2xBsaI_NT (Guide Stuffer) crRNA
Tn7 transposon
GmR+sfGFP VcDART Cargo

Expression

Bacterial

Promoter

PLac

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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eeBxb1

Plasmid

#222339

Purpose

Plasmid for expressing evolved and engineered Bxb1 in the mammalian cells

Depositor

David Liu

Article

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

Codon-optimized evolved and engineered Bxb1

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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CMV-Mito4x-GCaMP6f

Plasmid

#127870

Purpose

Fluorescent reporter for mitochondrial matrix Ca2+

Depositor

Timothy Ryan

Article

Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

Insert

4 repeats of COX8 signal peptide plus GCaMP6f

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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PPRE X3-TK-luc

Plasmid

#1015

Purpose

PPAR reporter. 3xDR1 sites upstream of a luciferase reporter.

Depositor

Bruce Spiegelman

Article

Kim et al Proc Natl Acad Sci U S A 1998 Apr 14;95(

Insert

PPRE

Use

Luciferase

Expression

Mammalian

Available Since

Nov. 3, 2005

Availability

Academic Institutions and Nonprofits only

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GFP-Rab5B

Plasmid

#61802

Purpose

Human Rab5B fluorescently tagged with GFP on the N-terminus for mammalian expression

Depositor

Gia Voeltz

Article

Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

Insert

RAB5B (RAB5B Human)

Tags

GFP

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3 Basic Vector

Plasmid

#212936

Purpose

Firefly luciferase vector for investigating regions controlling transcription

Depositor

Debrya Groskreutz

Type

Empty backbone

Use

Luciferase

Mutation

WT

Available Since

Jan. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHAGE-ESR1-Y537S

Plasmid

#116374

Purpose

Lentiviral expression of ESR1 Y537S

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ESR1 (ESR1 Human)

Use

Lentiviral

Mutation

Y537S

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-PE7

Plasmid

#214812

Purpose

Mammalian expression of SpCas9 PE7 prime editor

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pTXB1-Tn5

Plasmid

#60240

Purpose

IPTG-inducible expression of Transposon Tn5 fused to Mxe Intein and Chitin-binding domain.

Depositor

Rickard Sandberg

Article

Picelli et al Genome Res. 2014 Jul 30. pii: gr.177881.

Insert

tnpA from pBam1

Tags

Mxe intein - Chitin-binding domain

Expression

Bacterial

Mutation

E54K, L372P

Promoter

T7 promoter

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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lenti dCAS-VP64_Blast

Plasmid

#61425

Purpose

3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE)

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Has Service

Lentiviral Prep

Insert

dCAS9(D10A, N863A)-VP64_2A_Blast

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and N863A in Cas9

Promoter

EF1A

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV R-CEPIA3mt

Plasmid

#140464

Purpose

Red fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)

Depositor

Masamitsu Iino

Article

Kanemaru et al Sci Rep. 2020 Feb 18;10(1):2835. doi: 10

Insert

R-CEPIA3mt

Tags

Myc

Expression

Mammalian

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-mitoGFP

Plasmid

#44385

Depositor

Pantelis Tsoulfas

Article

Kitay et al Hum Mol Genet. 2013 Jan 24.

Insert

mitoGFP (COX8A Human)

Use

Lentiviral

Tags

Cox8 targeting sequence and GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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pV238-vPE

Plasmid

#225258

Purpose

Mammalian expression of vPE

Depositor

Vikash Chauhan, Robert Langer, Phil Sharp

Article

Chauhan et al Nature. 2025 Sep 17. doi: 10.1038/s41586

Insert

vPE

Use

CRISPR

Expression

Mammalian

Mutation

R221K K848A H982A N1317R

Promoter

CMV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pSBbi 41BBL-CD86 mIL-15

Plasmid

#183245

Purpose

Expression of human 41BBL, CD86, and membrane bound human IL-15 (IL-15-IL-15Ra) in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells

Depositor

Alexander McLellan

Article

Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

Insert

41BBL, CD86, mbIL-15 (IL-15-IL-15Ra)

Expression

Mammalian

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pKC117_pLenti_TetOff_MVP_IRES_PABPC1_INT

Plasmid

#242113

Purpose

Tet- or Dox-repressible expression of MVP and PABPC1 fused to INT (human PARP4 fragment)

Depositor

Fei Chen

Article

Chao et al Science. 2026 Jan 15:eadz9353. doi: 10.1

Insert

MVP and PAB4L-PARP4 fragment (MVP, PABPC1 Synthetic)

Use

Lentiviral

Mutation

PABPC1 aa 1-370, PARP4 aa 1562-1724

Promoter

TRE3G

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pSIRV-NF-kB-eCFP

Plasmid

#118094

Purpose

Flourescent reporter for NF-kB activity

Depositor

Peter Steinberger

Article

Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

Insert

eCFP

Use

Retroviral

Promoter

minimal promoter with NF-kB responsive element

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA

Plasmid

#61591

Purpose

A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.

Depositor

Feng Zhang

Article

Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

Inserts

hSaCas9
Chimeric guide for SaCas9

Use

AAV and CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Available Since

Feb. 16, 2015

Availability

Academic Institutions and Nonprofits only

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