Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

171,784 results

Showing: 61 - 80 of 171784 results

  3. mCherry-Golgi-7

    Plasmid
    #55052
    Purpose
    Localization: Golgi Complex, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Golgi (B4GALT1 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    aa 1-82 of NM_001497.3
    Promoter
    CMV
    Available Since
    Oct. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    55052-plasmid-map-sequence-id-352458

    Enlarge

  7. pGP-AAV-syn-FLEX-Volton2-ST-WPRE

    Plasmid
    #172907
    Purpose
    AAV-mediated expression of voltage sensor under the Syn promoter, Cre-dependent expression; soma localization targeting sequence
    Depositor
    GENIE Project
    Article
    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-
    Has Service
    AAV1
    Insert
    Voltron2-ST
    Use
    AAV and Cre/Lox
    Tags
    soma localization targeting sequence
    Expression
    Mammalian
    Mutation
    A122D
    Promoter
    hSynapsin1
    Available Since
    Feb. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    172907-plasmid-map-sequence-id-353194

    Enlarge

  12. hCas9_D10A

    Plasmid
    #41816
    Purpose
    Expresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineering
    Depositor
    George Church
    Article
    Mali et al Science. 2013 Feb 15;339(6121):823-6. do
    Insert
    Cas9_D10A
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A nickase
    Promoter
    CMV
    Available Since
    Jan. 11, 2013
    Availability
    Academic Institutions and Nonprofits only
    41816-plasmid-map-sequence-id-116715

    Enlarge

  14. 3` CC: Hygro – H2B-GFP-N – loxP – mScarlet

    Plasmid
    #219565
    Purpose
    3` circularization cassette to induce Cre-mediated circularization of a genomic region flanked by loxP sites with H2B-GFP reconstitution and mScarlet expression from the chromosome
    Depositor
    Andrea Ventura
    Article
    Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586
    Insert
    HygroR_2A_H2B-GFP-N_loxP_mScarlet
    Use
    Unspecified
    Promoter
    EF-1a
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    219565-plasmid-map-sequence-id-438331

    Enlarge

  16. pVP144

    Plasmid
    #222081
    Purpose
    Single component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion and eforRED for plasmid eviction.
    Depositor
    Wayne Parrott
    Article
    Pennetti et al Biodes Res. 2025 Feb 27;7(1):100001. doi
    Insert
    dCas9-PmCDA1, sfGFP, sacB and eforRED
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    Jan. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    222081-plasmid-map-sequence-id-438346

    Enlarge

  17. lenti sgRNA(MS2)_puro optimized backbone

    Plasmid
    #73797
    Purpose
    optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available Since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73797-plasmid-map-sequence-id-196924

    Enlarge

  19. 3` CC: Hygro – GFP-N – loxP – mScarlet

    Plasmid
    #219564
    Purpose
    3` circularization cassette.To induce Cre-mediated circularization or inversion of a genomic region with concomitant GFP reconstitution and mScarlet expression from the chromosome
    Depositor
    Andrea Ventura
    Article
    Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586
    Insert
    3` CC: Hygro – GFP-N – loxP – mScarlet
    Use
    Unspecified
    Promoter
    EF1-a
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    219564-plasmid-map-sequence-id-438335

    Enlarge

Showing: 61 - 80 of 171784 results