hCas9 Citations (409)

Originally described in: RNA-Guided Human Genome Engineering via Cas9.
Mali P, Yang L, Esvelt KM, Aach J, Guell M, Dicarlo JE, Norville JE, Church GM Science. 2013 Jan 3. PubMed Journal

Articles Citing hCas9

Articles
Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. Farzadfard F, Perli SD, Lu TK. ACS Synth Biol. 2013 Sep 11. PubMed Associated Plasmids pTPGI_dCas9_VP64 pRPR1_gRNA_handle_RPR1t pCMV_dCas9_VP64 pU6_gRNA_handle_U6t pRPR1(1xTetO)_gRNA_handle_RPR1t More ...
Efficient generation of large-scale genome-modified mice using gRNA and CAS9 endonuclease. Fujii W, Kawasaki K, Sugiura K, Naito K. Nucleic Acids Res. 2013 Aug 30. PubMed Associated Plasmids pCAG-T3-hCAS-pA pCAG-T3-hCASeGFP-pA
A simplified and efficient germline-specific CRISPR/Cas9 system for Drosophila genomic engineering. Sebo ZL, Lee HB, Peng Y, Guo Y. Fly (Austin). 2014;8(1):52-7. doi: 10.4161/fly.26828. Epub 2013 Oct 18. PubMed
CRISPR/Cas9-mediated targeted mutagenesis in the liverwort Marchantia polymorpha L. Sugano SS, Shirakawa M, Takagi J, Matsuda Y, Shimada T, Hara-Nishimura I, Kohchi T. Plant Cell Physiol. 2014 Mar;55(3):475-81. doi: 10.1093/pcp/pcu014. Epub 2014 Jan 18. PubMed
CRISPR-Cas-mediated targeted genome editing in human cells. Yang L, Mali P, Kim-Kiselak C, Church G. Methods Mol Biol. 2014;1114:245-67. doi: 10.1007/978-1-62703-761-7_16. PubMed
Role of mouse and human autophagy proteins in IFN-gamma-induced cell-autonomous responses against Toxoplasma gondii. Ohshima J, Lee Y, Sasai M, Saitoh T, Su Ma J, Kamiyama N, Matsuura Y, Pann-Ghill S, Hayashi M, Ebisu S, Takeda K, Akira S, Yamamoto M. J Immunol. 2014 Apr 1;192(7):3328-35. doi: 10.4049/jimmunol.1302822. Epub 2014 Feb 21. PubMed
High-precision robotic microcontact printing (R-muCP) utilizing a vision guided selectively compliant articulated robotic arm. McNulty JD, Klann T, Sha J, Salick M, Knight GT, Turng LS, Ashton RS. Lab Chip. 2014 Jun 7;14(11):1923-30. doi: 10.1039/c3lc51137e. Epub 2014 Apr 24. PubMed
A high-coverage shRNA screen identifies TMEM129 as an E3 ligase involved in ER-associated protein degradation. van de Weijer ML, Bassik MC, Luteijn RD, Voorburg CM, Lohuis MA, Kremmer E, Hoeben RC, LeProust EM, Chen S, Hoelen H, Ressing ME, Patena W, Weissman JS, McManus MT, Wiertz EJ, Lebbink RJ. Nat Commun. 2014 May 8;5:3832. doi: 10.1038/ncomms4832. PubMed
Adenoviral vector delivery of RNA-guided CRISPR/Cas9 nuclease complexes induces targeted mutagenesis in a diverse array of human cells. Maggio I, Holkers M, Liu J, Janssen JM, Chen X, Goncalves MA. Sci Rep. 2014 May 29;4:5105. doi: 10.1038/srep05105. PubMed Associated Plasmids pAdSh.U6.gRNAS1 pAdSh.PGK.Cas9 pAdSh.U6.gRNAGFP
Engineering human tumour-associated chromosomal translocations with the RNA-guided CRISPR-Cas9 system. Torres R, Martin MC, Garcia A, Cigudosa JC, Ramirez JC, Rodriguez-Perales S. Nat Commun. 2014 Jun 3;5:3964. doi: 10.1038/ncomms4964. PubMed
Biallelic targeting of expressed genes in mouse embryonic stem cells using the Cas9 system. Zhang Y, Vanoli F, LaRocque JR, Krawczyk PM, Jasin M. Methods. 2014 Sep;69(2):171-8. doi: 10.1016/j.ymeth.2014.05.003. Epub 2014 Jun 12. PubMed
Rapid generation of mouse models with defined point mutations by the CRISPR/Cas9 system. Inui M, Miyado M, Igarashi M, Tamano M, Kubo A, Yamashita S, Asahara H, Fukami M, Takada S. Sci Rep. 2014 Jun 23;4:5396. doi: 10.1038/srep05396. PubMed
Allele-specific genome editing and correction of disease-associated phenotypes in rats using the CRISPR-Cas platform. Yoshimi K, Kaneko T, Voigt B, Mashimo T. Nat Commun. 2014 Jun 26;5:4240. doi: 10.1038/ncomms5240. PubMed Associated Plasmids pT7-gRNA:Tyr (albino) pT7-gRNA:Tyr (wild) pT7-gRNA:Asip pT7-gRNA:kit-1 pT7-gRNA:kit-2-1 More ...
CRISPR/Cas9-Directed Genome Editing of Cultured Cells. Yang L, Yang JL, Byrne S, Pan J, Church GM. Curr Protoc Mol Biol. 2014 Jul 1;107:31.1.1-31.1.17. doi: 10.1002/0471142727.mb3101s107. PubMed
Whole-genome sequencing analysis reveals high specificity of CRISPR/Cas9 and TALEN-based genome editing in human iPSCs. Smith C, Gore A, Yan W, Abalde-Atristain L, Li Z, He C, Wang Y, Brodsky RA, Zhang K, Cheng L, Ye Z. Cell Stem Cell. 2014 Jul 3;15(1):12-3. doi: 10.1016/j.stem.2014.06.011. PubMed
A single blastocyst assay optimized for detecting CRISPR/Cas9 system-induced indel mutations in mice. Sakurai T, Watanabe S, Kamiyoshi A, Sato M, Shindo T. BMC Biotechnol. 2014 Jul 21;14:69. doi: 10.1186/1472-6750-14-69. PubMed
PVT1 dependence in cancer with MYC copy-number increase. Tseng YY, Moriarity BS, Gong W, Akiyama R, Tiwari A, Kawakami H, Ronning P, Reuland B, Guenther K, Beadnell TC, Essig J, Otto GM, O'Sullivan MG, Largaespada DA, Schwertfeger KL, Marahrens Y, Kawakami Y, Bagchi A. Nature. 2014 Aug 7;512(7512):82-6. doi: 10.1038/nature13311. Epub 2014 Jun 22. PubMed
In vitro and in vivo growth suppression of human papillomavirus 16-positive cervical cancer cells by CRISPR/Cas9. Zhen S, Hua L, Takahashi Y, Narita S, Liu YH, Li Y. Biochem Biophys Res Commun. 2014 Aug 8;450(4):1422-6. doi: 10.1016/j.bbrc.2014.07.014. Epub 2014 Jul 17. PubMed
Using RNA-seq and targeted nucleases to identify mechanisms of drug resistance in acute myeloid leukemia. Rathe SK, Moriarity BS, Stoltenberg CB, Kurata M, Aumann NK, Rahrmann EP, Bailey NJ, Melrose EG, Beckmann DA, Liska CR, Largaespada DA. Sci Rep. 2014 Aug 13;4:6048. doi: 10.1038/srep06048. PubMed
Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector. Kabadi AM, Ousterout DG, Hilton IB, Gersbach CA. Nucleic Acids Res. 2014 Aug 13. pii: gku749. PubMed Associated Plasmids phH1-gRNA pmU6-gRNA phU6-gRNA ph7SK-gRNA pLV hUbC-Cas9-T2A-GFP More ...
Prevention of muscular dystrophy in mice by CRISPR/Cas9-mediated editing of germline DNA. Long C, McAnally JR, Shelton JM, Mireault AA, Bassel-Duby R, Olson EN. Science. 2014 Sep 5;345(6201):1184-1188. doi: 10.1126/science.1254445. Epub 2014 Aug 14. PubMed
The CRISPR/Cas9 System Facilitates Clearance of the Intrahepatic HBV Templates In Vivo. Lin SR, Yang HC, Kuo YT, Liu CJ, Yang TY, Sung KC, Lin YY, Wang HY, Wang CC, Shen YC, Wu FY, Kao JH, Chen DS, Chen PJ. Mol Ther Nucleic Acids. 2014 Aug 19;3:e186. doi: 10.1038/mtna.2014.38. PubMed
Adenoviral vector DNA for accurate genome editing with engineered nucleases. Holkers M, Maggio I, Henriques SF, Janssen JM, Cathomen T, Goncalves MA. Nat Methods. 2014 Oct;11(10):1051-7. doi: 10.1038/nmeth.3075. Epub 2014 Aug 24. PubMed
Nascent RNA interaction keeps PRC2 activity poised and in check. Kaneko S, Son J, Bonasio R, Shen SS, Reinberg D. Genes Dev. 2014 Sep 15;28(18):1983-8. doi: 10.1101/gad.247940.114. Epub 2014 Aug 28. PubMed
Improving the specificity and efficacy of CRISPR/CAS9 and gRNA through target specific DNA reporter. Zhang JH, Pandey M, Kahler JF, Loshakov A, Harris B, Dagur PK, Mo Y, Simonds WF. J Biotechnol. 2014 Sep 2. pii: S0168-1656(14)00838-4. doi: 10.1016/j.jbiotec.2014.08.033. PubMed Associated Plasmids psAAVS1-T2
Precise gene deletion and replacement using the CRISPR/Cas9 system in human cells. Zheng Q, Cai X, Tan MH, Schaffert S, Arnold CP, Gong X, Chen CZ, Huang S. Biotechniques. 2014 Sep 1;57(3):115-24. doi: 10.2144/000114196. eCollection 2014. PubMed Associated Plasmids pUC-H1-gRNA
Simple knockout by electroporation of engineered endonucleases into intact rat embryos. Kaneko T, Sakuma T, Yamamoto T, Mashimo T. Sci Rep. 2014 Oct 1;4:6382. doi: 10.1038/srep06382. PubMed
Mouse Genome Editing Using the CRISPR/Cas System. Harms DW, Quadros RM, Seruggia D, Ohtsuka M, Takahashi G, Montoliu L, Gurumurthy CB. Curr Protoc Hum Genet. 2014 Oct 1;83:15.7.1-15.7.27. doi: 10.1002/0471142905.hg1507s83. PubMed Associated Plasmids pBGK
Generation of CRISPR/Cas9-mediated gene-targeted pigs via somatic cell nuclear transfer. Zhou X, Xin J, Fan N, Zou Q, Huang J, Ouyang Z, Zhao Y, Zhao B, Liu Z, Lai S, Yi X, Guo L, Esteban MA, Zeng Y, Yang H, Lai L. Cell Mol Life Sci. 2015 Mar;72(6):1175-84. doi: 10.1007/s00018-014-1744-7. Epub 2014 Oct 2. PubMed
Improved genome editing in human cell lines using the CRISPR method. Munoz IM, Szyniarowski P, Toth R, Rouse J, Lachaud C. PLoS One. 2014 Oct 10;9(10):e109752. doi: 10.1371/journal.pone.0109752. eCollection 2014. PubMed
IFI16 restricts HSV-1 replication by accumulating on the hsv-1 genome, repressing HSV-1 gene expression, and directly or indirectly modulating histone modifications. Johnson KE, Bottero V, Flaherty S, Dutta S, Singh VV, Chandran B. PLoS Pathog. 2014 Nov 6;10(11):e1004503. doi: 10.1371/journal.ppat.1004503. eCollection 2014 Nov. PubMed
Off-target assessment of CRISPR-Cas9 guiding RNAs in human iPS and mouse ES cells. Tan EP, Li Y, Velasco-Herrera Mdel C, Yusa K, Bradley A. Genesis. 2015 Feb;53(2):225-36. doi: 10.1002/dvg.22835. Epub 2014 Dec 10. PubMed
Assaying break and nick-induced homologous recombination in mammalian cells using the DR-GFP reporter and Cas9 nucleases. Vriend LE, Jasin M, Krawczyk PM. Methods Enzymol. 2014;546:175-91. doi: 10.1016/B978-0-12-801185-0.00009-X. PubMed
Efficient and allele-specific genome editing of disease loci in human iPSCs. Smith C, Abalde-Atristain L, He C, Brodsky BR, Braunstein EM, Chaudhari P, Jang YY, Cheng L, Ye Z. Mol Ther. 2015 Mar;23(3):570-7. doi: 10.1038/mt.2014.226. Epub 2014 Nov 24. PubMed
Kinetic pathway of 40S ribosomal subunit recruitment to hepatitis C virus internal ribosome entry site. Fuchs G, Petrov AN, Marceau CD, Popov LM, Chen J, O'Leary SE, Wang R, Carette JE, Sarnow P, Puglisi JD. Proc Natl Acad Sci U S A. 2015 Jan 13;112(2):319-25. doi: 10.1073/pnas.1421328111. Epub 2014 Dec 16. PubMed
Fanconi anemia gene editing by the CRISPR/Cas9 system. Osborn MJ, Gabriel R, Webber BR, DeFeo AP, McElroy AN, Jarjour J, Starker CG, Wagner JE, Joung JK, Voytas DF, von Kalle C, Schmidt M, Blazar BR, Tolar J. Hum Gene Ther. 2015 Feb;26(2):114-26. doi: 10.1089/hum.2014.111. PubMed
Gene Targeting Through Homologous Recombination in Monkey Embryonic Stem Cells Using CRISPR/Cas9 System. Zhu S, Rong Z, Lu X, Xu Y, Fu X. Stem Cells Dev. 2015 May 15;24(10):1147-9. doi: 10.1089/scd.2014.0507. Epub 2015 Feb 25. PubMed
Harnessing the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated Cas9 system to disrupt the hepatitis B virus. Zhen S, Hua L, Liu YH, Gao LC, Fu J, Wan DY, Dong LH, Song HF, Gao X. Gene Ther. 2015 May;22(5):404-12. doi: 10.1038/gt.2015.2. Epub 2015 Feb 5. PubMed
Production of Gene-Corrected Adult Beta Globin Protein in Human Erythrocytes Differentiated from Patient iPSCs After Genome Editing of the Sickle Point Mutation. Huang X, Wang Y, Yan W, Smith C, Ye Z, Wang J, Gao Y, Mendelsohn L, Cheng L. Stem Cells. 2015 May;33(5):1470-9. doi: 10.1002/stem.1969. PubMed
A genome-wide analysis of Cas9 binding specificity using ChIP-seq and targeted sequence capture. O'Geen H, Henry IM, Bhakta MS, Meckler JF, Segal DJ. Nucleic Acids Res. 2015 Mar 31;43(6):3389-404. doi: 10.1093/nar/gkv137. Epub 2015 Feb 20. PubMed
CRISPR/Cas9 as tool for functional study of genes involved in preimplantation embryo development. Kwon J, Namgoong S, Kim NH. PLoS One. 2015 Mar 16;10(3):e0120501. doi: 10.1371/journal.pone.0120501. eCollection 2015. PubMed
Selection of a hepatitis C virus with altered entry factor requirements reveals a genetic interaction between the E1 glycoprotein and claudins. Hopcraft SE, Evans MJ. Hepatology. 2015 Mar 29. doi: 10.1002/hep.27815. PubMed
Interaction of the Clostridium difficile Binary Toxin CDT and Its Host Cell Receptor, Lipolysis-stimulated Lipoprotein Receptor (LSR). Hemmasi S, Czulkies BA, Schorch B, Veit A, Aktories K, Papatheodorou P. J Biol Chem. 2015 May 29;290(22):14031-44. doi: 10.1074/jbc.M115.650523. Epub 2015 Apr 16. PubMed
Functional validation of mouse tyrosinase non-coding regulatory DNA elements by CRISPR-Cas9-mediated mutagenesis. Seruggia D, Fernandez A, Cantero M, Pelczar P, Montoliu L. Nucleic Acids Res. 2015 May 26;43(10):4855-67. doi: 10.1093/nar/gkv375. Epub 2015 Apr 20. PubMed
Nanoparticles that deliver triplex-forming peptide nucleic acid molecules correct F508del CFTR in airway epithelium. McNeer NA, Anandalingam K, Fields RJ, Caputo C, Kopic S, Gupta A, Quijano E, Polikoff L, Kong Y, Bahal R, Geibel JP, Glazer PM, Saltzman WM, Egan ME. Nat Commun. 2015 Apr 27;6:6952. doi: 10.1038/ncomms7952. PubMed
Cas9-mediated targeting of viral RNA in eukaryotic cells. Price AA, Sampson TR, Ratner HK, Grakoui A, Weiss DS. Proc Natl Acad Sci U S A. 2015 May 12;112(19):6164-9. doi: 10.1073/pnas.1422340112. Epub 2015 Apr 27. PubMed
Sequential cancer mutations in cultured human intestinal stem cells. Drost J, van Jaarsveld RH, Ponsioen B, Zimberlin C, van Boxtel R, Buijs A, Sachs N, Overmeer RM, Offerhaus GJ, Begthel H, Korving J, van de Wetering M, Schwank G, Logtenberg M, Cuppen E, Snippert HJ, Medema JP, Kops GJ, Clevers H. Nature. 2015 May 7;521(7550):43-7. doi: 10.1038/nature14415. Epub 2015 Apr 29. PubMed
An alternative pluripotent state confers interspecies chimaeric competency. Wu J, Okamura D, Li M, Suzuki K, Luo C, Ma L, He Y, Li Z, Benner C, Tamura I, Krause MN, Nery JR, Du T, Zhang Z, Hishida T, Takahashi Y, Aizawa E, Kim NY, Lajara J, Guillen P, Campistol JM, Esteban CR, Ross PJ, Saghatelian A, Ren B, Ecker JR, Izpisua Belmonte JC. Nature. 2015 May 21;521(7552):316-21. doi: 10.1038/nature14413. Epub 2015 May 6. PubMed
The Nuclear DNA Sensor IFI16 Acts as a Restriction Factor for Human Papillomavirus Replication through Epigenetic Modifications of the Viral Promoters. Lo Cigno I, De Andrea M, Borgogna C, Albertini S, Landini MM, Peretti A, Johnson KE, Chandran B, Landolfo S, Gariglio M. J Virol. 2015 Aug 1;89(15):7506-20. doi: 10.1128/JVI.00013-15. Epub 2015 May 13. PubMed
ATRX represses alternative lengthening of telomeres. Napier CE, Huschtscha LI, Harvey A, Bower K, Noble JR, Hendrickson EA, Reddel RR. Oncotarget. 2015 Jun 30;6(18):16543-58. PubMed
Modulation of Gene Expression by Polymer Nanocapsule Delivery of DNA Cassettes Encoding Small RNAs. Yan M, Wen J, Liang M, Lu Y, Kamata M, Chen IS. PLoS One. 2015 Jun 2;10(6):e0127986. doi: 10.1371/journal.pone.0127986. eCollection 2015. PubMed
Generation of mutant mice via the CRISPR/Cas9 system using FokI-dCas9. Hara S, Tamano M, Yamashita S, Kato T, Saito T, Sakuma T, Yamamoto T, Inui M, Takada S. Sci Rep. 2015 Jun 9;5:11221. doi: 10.1038/srep11221. PubMed
Molecular characterization of LC3-associated phagocytosis reveals distinct roles for Rubicon, NOX2 and autophagy proteins. Martinez J, Malireddi RK, Lu Q, Cunha LD, Pelletier S, Gingras S, Orchard R, Guan JL, Tan H, Peng J, Kanneganti TD, Virgin HW, Green DR. Nat Cell Biol. 2015 Jul;17(7):893-906. doi: 10.1038/ncb3192. Epub 2015 Jun 22. PubMed
Haploid Genetic Screen Reveals a Profound and Direct Dependence on Cholesterol for Hantavirus Membrane Fusion. Kleinfelter LM, Jangra RK, Jae LT, Herbert AS, Mittler E, Stiles KM, Wirchnianski AS, Kielian M, Brummelkamp TR, Dye JM, Chandran K. MBio. 2015 Jun 30;6(4):e00801. doi: 10.1128/mBio.00801-15. PubMed
CCAR2/DBC1 is required for Chk2-dependent KAP1 phosphorylation and repair of DNA damage. Magni M, Ruscica V, Restelli M, Fontanella E, Buscemi G, Zannini L. Oncotarget. 2015 Jul 10;6(19):17817-31. PubMed
MiT/TFE transcription factors are activated during mitophagy downstream of Parkin and Atg5. Nezich CL, Wang C, Fogel AI, Youle RJ. J Cell Biol. 2015 Aug 3;210(3):435-50. doi: 10.1083/jcb.201501002. PubMed
Direct Injection of CRISPR/Cas9-Related mRNA into Cytoplasm of Parthenogenetically Activated Porcine Oocytes Causes Frequent Mosaicism for Indel Mutations. Sato M, Koriyama M, Watanabe S, Ohtsuka M, Sakurai T, Inada E, Saitoh I, Nakamura S, Miyoshi K. Int J Mol Sci. 2015 Aug 3;16(8):17838-56. doi: 10.3390/ijms160817838. PubMed
CRISPR mediated somatic cell genome engineering in the chicken. Veron N, Qu Z, Kipen PA, Hirst CE, Marcelle C. Dev Biol. 2015 Nov 1;407(1):68-74. doi: 10.1016/j.ydbio.2015.08.007. Epub 2015 Aug 13. PubMed
Cell-Cycle Control of Bivalent Epigenetic Domains Regulates the Exit from Pluripotency. Singh AM, Sun Y, Li L, Zhang W, Wu T, Zhao S, Qin Z, Dalton S. Stem Cell Reports. 2015 Sep 8;5(3):323-36. doi: 10.1016/j.stemcr.2015.07.005. Epub 2015 Aug 13. PubMed
Knockout of cGAS and STING Rescues Virus Infection of Plasmid DNA-Transfected Cells. Langereis MA, Rabouw HH, Holwerda M, Visser LJ, van Kuppeveld FJ. J Virol. 2015 Nov;89(21):11169-73. doi: 10.1128/JVI.01781-15. Epub 2015 Aug 26. PubMed
Cas9 gRNA engineering for genome editing, activation and repression. Kiani S, Chavez A, Tuttle M, Hall RN, Chari R, Ter-Ovanesyan D, Qian J, Pruitt BW, Beal J, Vora S, Buchthal J, Kowal EJ, Ebrahimkhani MR, Collins JJ, Weiss R, Church G. Nat Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580. PubMed Associated Plasmids AAV_NLS-dSaCas9-NLS-VPR AAV_NLS-SaCas9-NLS-VPR hCas9-VPR M-ST1-VPR gRNA_tdtomato_reporter_1_ST1_2x_binding More ...
Research of methods to detect genomic mutations induced by CRISPR/Cas systems. Wang K, Mei DY, Liu QN, Qiao XH, Ruan WM, Huang T, Cao GS. J Biotechnol. 2015 Nov 20;214:128-32. doi: 10.1016/j.jbiotec.2015.09.029. Epub 2015 Sep 28. PubMed
Use of the HPRT gene to study nuclease-induced DNA double-strand break repair. Gravells P, Ahrabi S, Vangala RK, Tomita K, Brash JT, Brustle LA, Chung C, Hong JM, Kaloudi A, Humphrey TC, Porter AC. Hum Mol Genet. 2015 Dec 15;24(24):7097-110. doi: 10.1093/hmg/ddv409. Epub 2015 Sep 30. PubMed
Monoclonal antibody profiling of cell surface proteins associated with the viral biofilms on HTLV-1 transformed cells. Tarasevich A, Filatov A, Pichugin A, Mazurov D. Acta Virol. 2015 Sep;59(3):247-56. PubMed
Homology-directed repair in rodent zygotes using Cas9 and TALEN engineered proteins. Menoret S, De Cian A, Tesson L, Remy S, Usal C, Boule JB, Boix C, Fontaniere S, Creneguy A, Nguyen TH, Brusselle L, Thinard R, Gauguier D, Concordet JP, Cherifi Y, Fraichard A, Giovannangeli C, Anegon I. Sci Rep. 2015 Oct 7;5:14410. doi: 10.1038/srep14410. PubMed
Disruption of MeCP2 attenuates circadian rhythm in CRISPR/Cas9-based Rett syndrome model mouse. Tsuchiya Y, Minami Y, Umemura Y, Watanabe H, Ono D, Nakamura W, Takahashi T, Honma S, Kondoh G, Matsuishi T, Yagita K. Genes Cells. 2015 Dec;20(12):992-1005. doi: 10.1111/gtc.12305. Epub 2015 Oct 12. PubMed
Glucocorticoid receptor antagonism reverts docetaxel resistance in human prostate cancer. Kroon J, Puhr M, Buijs JT, van der Horst G, Hemmer DM, Marijt KA, Hwang MS, Masood M, Grimm S, Storm G, Metselaar JM, Meijer OC, Culig Z, van der Pluijm G. Endocr Relat Cancer. 2016 Jan;23(1):35-45. doi: 10.1530/ERC-15-0343. Epub 2015 Oct 19. PubMed
Effect of Multiple Clock Gene Ablations on the Circadian Period Length and Temperature Compensation in Mammalian Cells. Tsuchiya Y, Umemura Y, Minami Y, Koike N, Hosokawa T, Hara M, Ito H, Inokawa H, Yagita K. J Biol Rhythms. 2016 Feb;31(1):48-56. doi: 10.1177/0748730415613888. Epub 2015 Oct 28. PubMed
Simple Genome Editing of Rodent Intact Embryos by Electroporation. Kaneko T, Mashimo T. PLoS One. 2015 Nov 10;10(11):e0142755. doi: 10.1371/journal.pone.0142755. eCollection 2015. PubMed
Differentiation of human ESCs to retinal ganglion cells using a CRISPR engineered reporter cell line. Sluch VM, Davis CH, Ranganathan V, Kerr JM, Krick K, Martin R, Berlinicke CA, Marsh-Armstrong N, Diamond JS, Mao HQ, Zack DJ. Sci Rep. 2015 Nov 13;5:16595. doi: 10.1038/srep16595. PubMed
Simultaneous Reprogramming and Gene Correction of Patient Fibroblasts. Howden SE, Maufort JP, Duffin BM, Elefanty AG, Stanley EG, Thomson JA. Stem Cell Reports. 2015 Dec 8;5(6):1109-18. doi: 10.1016/j.stemcr.2015.10.009. Epub 2015 Nov 12. PubMed Associated Plasmids pSimple-miR302/367
Impaired PRC2 activity promotes transcriptional instability and favors breast tumorigenesis. Wassef M, Rodilla V, Teissandier A, Zeitouni B, Gruel N, Sadacca B, Irondelle M, Charruel M, Ducos B, Michaud A, Caron M, Marangoni E, Chavrier P, Le Tourneau C, Kamal M, Pasmant E, Vidaud M, Servant N, Reyal F, Meseure D, Vincent-Salomon A, Fre S, Margueron R. Genes Dev. 2015 Dec 15;29(24):2547-62. doi: 10.1101/gad.269522.115. Epub 2015 Dec 4. PubMed
Atg13 Is Essential for Autophagy and Cardiac Development in Mice. Kaizuka T, Mizushima N. Mol Cell Biol. 2015 Dec 7;36(4):585-95. doi: 10.1128/MCB.01005-15. PubMed
Genome editing through large insertion leads to the skipping of targeted exon. Uddin B, Chen NP, Panic M, Schiebel E. BMC Genomics. 2015 Dec 21;16(1):1082. doi: 10.1186/s12864-015-2284-8. PubMed
Filovirus receptor NPC1 contributes to species-specific patterns of ebolavirus susceptibility in bats. Ng M, Ndungo E, Kaczmarek ME, Herbert AS, Binger T, Kuehne AI, Jangra RK, Hawkins JA, Gifford RJ, Biswas R, Demogines A, James RM, Yu M, Brummelkamp TR, Drosten C, Wang LF, Kuhn JH, Muller MA, Dye JM, Sawyer SL, Chandran K. Elife. 2015 Dec 23;4. pii: e11785. doi: 10.7554/eLife.11785. PubMed
A possible aid in targeted insertion of large DNA elements by CRISPR/Cas in mouse zygotes. Nakao H, Harada T, Nakao K, Kiyonari H, Inoue K, Furuta Y, Aiba A. Genesis. 2015 Dec 29. doi: 10.1002/dvg.22914. PubMed
High content analysis platform for optimization of lipid mediated CRISPR-Cas9 delivery strategies in human cells. Steyer B, Carlson-Stevermer J, Angenent-Mari N, Khalil A, Harkness T, Saha K. Acta Biomater. 2015 Dec 30. pii: S1742-7061(15)30273-7. doi: 10.1016/j.actbio.2015.12.036. PubMed
Generation of Knock-In Pigs Carrying Oct4-tdTomato Reporter through CRISPR/Cas9-Mediated Genome Engineering. Lai S, Wei S, Zhao B, Ouyang Z, Zhang Q, Fan N, Liu Z, Zhao Y, Yan Q, Zhou X, Li L, Xin J, Zeng Y, Lai L, Zou Q. PLoS One. 2016 Jan 12;11(1):e0146562. doi: 10.1371/journal.pone.0146562. eCollection 2016. PubMed
Selection-free gene repair after adenoviral vector transduction of designer nucleases: rescue of dystrophin synthesis in DMD muscle cell populations. Maggio I, Stefanucci L, Janssen JM, Liu J, Chen X, Mouly V, Goncalves MA. Nucleic Acids Res. 2016 Jan 13. pii: gkv1540. PubMed
Golgi-Resident GTPase Rab30 Promotes the Biogenesis of Pathogen-Containing Autophagosomes. Oda S, Nozawa T, Nozawa-Minowa A, Tanaka M, Aikawa C, Harada H, Nakagawa I. PLoS One. 2016 Jan 15;11(1):e0147061. doi: 10.1371/journal.pone.0147061. eCollection 2016. PubMed
Conversion of embryonic stem cells into extraembryonic lineages by CRISPR-mediated activators. Wei S, Zou Q, Lai S, Zhang Q, Li L, Yan Q, Zhou X, Zhong H, Lai L. Sci Rep. 2016 Jan 19;6:19648. doi: 10.1038/srep19648. PubMed
ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes. Yoshimi K, Kunihiro Y, Kaneko T, Nagahora H, Voigt B, Mashimo T. Nat Commun. 2016 Jan 20;7:10431. doi: 10.1038/ncomms10431. PubMed Associated Plasmids pCas9-polyA
Redundant function of DNA ligase 1 and 3 in alternative end-joining during immunoglobulin class switch recombination. Masani S, Han L, Meek K, Yu K. Proc Natl Acad Sci U S A. 2016 Feb 2;113(5):1261-6. doi: 10.1073/pnas.1521630113. Epub 2016 Jan 19. PubMed
Ligase I and ligase III mediate the DNA double-strand break ligation in alternative end-joining. Lu G, Duan J, Shu S, Wang X, Gao L, Guo J, Zhang Y. Proc Natl Acad Sci U S A. 2016 Feb 2;113(5):1256-60. doi: 10.1073/pnas.1521597113. Epub 2016 Jan 19. PubMed
Cancer-associated SF3B1 mutations affect alternative splicing by promoting alternative branchpoint usage. Alsafadi S, Houy A, Battistella A, Popova T, Wassef M, Henry E, Tirode F, Constantinou A, Piperno-Neumann S, Roman-Roman S, Dutertre M, Stern MH. Nat Commun. 2016 Feb 4;7:10615. doi: 10.1038/ncomms10615. PubMed
A Vector with a Single Promoter for In Vitro Transcription and Mammalian Cell Expression of CRISPR gRNAs. Romanienko PJ, Giacalone J, Ingenito J, Wang Y, Isaka M, Johnson T, You Y, Mark WH. PLoS One. 2016 Feb 5;11(2):e0148362. doi: 10.1371/journal.pone.0148362. eCollection 2016. PubMed Associated Plasmids pU6T7 pU6T7G
Knock-in of large reporter genes in human cells via CRISPR/Cas9-induced homology-dependent and independent DNA repair. He X, Tan C, Wang F, Wang Y, Zhou R, Cui D, You W, Zhao H, Ren J, Feng B. Nucleic Acids Res. 2016 May 19;44(9):e85. doi: 10.1093/nar/gkw064. Epub 2016 Feb 4. PubMed Associated Plasmids single cut NH-donor double cut NH-donor 2a-copGFP(+HA) Sg-A sgRNA 1 GAPDH More ...
Direct Visualization of Ebola Virus Fusion Triggering in the Endocytic Pathway. Spence JS, Krause TB, Mittler E, Jangra RK, Chandran K. MBio. 2016 Feb 9;7(1):e01857-15. doi: 10.1128/mBio.01857-15. PubMed
Induction of site-specific chromosomal translocations in embryonic stem cells by CRISPR/Cas9. Jiang J, Zhang L, Zhou X, Chen X, Huang G, Li F, Wang R, Wu N, Yan Y, Tong C, Srivastava S, Wang Y, Liu H, Ying QL. Sci Rep. 2016 Feb 22;6:21918. doi: 10.1038/srep21918. PubMed
Regulation of Transcription Factor Yin Yang 1 by SET7/9-mediated Lysine Methylation. Zhang WJ, Wu XN, Shi TT, Xu HT, Yi J, Shen HF, Huang MF, Shu XY, Wang FF, Peng BL, Xiao RQ, Gao WW, Ding JC, Liu W. Sci Rep. 2016 Feb 23;6:21718. doi: 10.1038/srep21718. PubMed
T cell-specific inactivation of mouse CD2 by CRISPR/Cas9. Beil-Wagner J, Dossinger G, Schober K, vom Berg J, Tresch A, Grandl M, Palle P, Mair F, Gerhard M, Becher B, Busch DH, Buch T. Sci Rep. 2016 Feb 23;6:21377. doi: 10.1038/srep21377. PubMed
Transglutaminase 2 contributes to a TP53-induced autophagy program to prevent oncogenic transformation. Yeo SY, Itahana Y, Guo AK, Han R, Iwamoto K, Nguyen HT, Bao Y, Kleiber K, Wu YJ, Bay BH, Voorhoeve M, Itahana K. Elife. 2016 Mar 9;5:e07101. doi: 10.7554/eLife.07101. PubMed
Utilization of the CRISPR/Cas9 system for the efficient production of mutant mice using crRNA/tracrRNA with Cas9 nickase and FokI-dCas9. Terao M, Tamano M, Hara S, Kato T, Kinoshita M, Takada S. Exp Anim. 2016 Mar 14. PubMed
Distinct genetic control of homologous recombination repair of Cas9-induced double-strand breaks, nicks and paired nicks. Vriend LE, Prakash R, Chen CC, Vanoli F, Cavallo F, Zhang Y, Jasin M, Krawczyk PM. Nucleic Acids Res. 2016 Jun 20;44(11):5204-17. doi: 10.1093/nar/gkw179. Epub 2016 Mar 21. PubMed
A Traceless Selection: Counter-selection System That Allows Efficient Generation of Transposon and CRISPR-modified T-cell Products. Mezzadra R, Hollenstein A, Gomez-Eerland R, Schumacher TN. Mol Ther Nucleic Acids. 2016 Mar 22;5:e298. doi: 10.1038/mtna.2016.13. PubMed
Efficient genome editing of genes involved in neural crest development using the CRISPR/Cas9 system in Xenopus embryos. Liu Z, Cheng TT, Shi Z, Liu Z, Lei Y, Wang C, Shi W, Chen X, Qi X, Cai D, Feng B, Deng Y, Chen Y, Zhao H. Cell Biosci. 2016 Mar 31;6:22. doi: 10.1186/s13578-016-0088-4. eCollection 2016. PubMed
pEVL: A Linear Plasmid for Generating mRNA IVT Templates With Extended Encoded Poly(A) Sequences. Grier AE, Burleigh S, Sahni J, Clough CA, Cardot V, Choe DC, Krutein MC, Rawlings DJ, Jensen MC, Scharenberg AM, Jacoby K. Mol Ther Nucleic Acids. 2016 Apr 19;5:e306. doi: 10.1038/mtna.2016.21. PubMed
Multiplexed Targeted Genome Engineering Using a Universal Nuclease-Assisted Vector Integration System. Brown A, Woods WS, Perez-Pinera P. ACS Synth Biol. 2016 Jul 15;5(7):582-8. doi: 10.1021/acssynbio.6b00056. Epub 2016 May 9. PubMed
High Efficiency CRISPR/Cas9-mediated Gene Editing in Primary Human T-cells Using Mutant Adenoviral E4orf6/E1b55k "Helper" Proteins. Gwiazda KS, Grier AE, Sahni J, Burleigh SM, Martin U, Yang JG, Popp NA, Krutein MC, Khan IF, Jacoby K, Jensen MC, Rawlings DJ, Scharenberg AM. Mol Ther. 2016 Jun 28. doi: 10.1038/mt.2016.105. PubMed
CRISPR-Cas9 mediated modification of the NOD mouse genome with Ptpn22R619W mutation increases autoimmune diabetes. Lin X, Pelletier S, Gingras S, Rigaud S, Maine CJ, Marquardt K, Dai YD, Sauer K, Rodriguez AR, Martin G, Kupriyanov S, Jiang L, Yu L, Green DR, Sherman LA. Diabetes. 2016 Apr 26. pii: db160061. PubMed
Requirement for the Mitochondrial Pyruvate Carrier in Mammalian Development Revealed by a Hypomorphic Allelic Series. Bowman CE, Zhao L, Hartung T, Wolfgang MJ. Mol Cell Biol. 2016 May 23. pii: MCB.00166-16. PubMed
Activity of Menkes Disease Protein ATP7A is Essential for Redox Balance in Mitochondria. Bhattacharjee A, Yang H, Duffy M, Robinson E, Conrad-Antoville A, Lu YW, Capps T, Braiterman LT, Wolfgang MJ, Murphy MP, Yi L, Kaler SG, Lutsenko S, Ralle M. J Biol Chem. 2016 May 16. pii: jbc.M116.727248. PubMed
Footprintless disruption of prosurvival genes in aneuploid cancer cells using CRISPR/Cas9 technology. Krachulec JM, Sedlmeier G, Thiele W, Sleeman JP. Biochem Cell Biol. 2016 Jun;94(3):289-96. doi: 10.1139/bcb-2015-0150. Epub 2016 Jun 2. PubMed
Probing the impact of chromatin conformation on genome editing tools. Chen X, Rinsma M, Janssen JM, Liu J, Maggio I, Goncalves MA. Nucleic Acids Res. 2016 Jun 8. pii: gkw524. PubMed Associated Plasmids AW01_pU.CAG.eSpCas9(1.1).rBGpA
Viral Determinants of miR-122-Independent Hepatitis C Virus Replication. Hopcraft SE, Azarm KD, Israelow B, Leveque N, Schwarz MC, Hsu TH, Chambers MT, Sourisseau M, Semler BL, Evans MJ. mSphere. 2015 Nov 25;1(1). pii: e00009-15. doi: 10.1128/mSphere.00009-15. eCollection 2016 Jan-Feb. PubMed
Engineering of Isogenic Cells Deficient for MR1 with a CRISPR/Cas9 Lentiviral System: Tools To Study Microbial Antigen Processing and Presentation to Human MR1-Restricted T Cells. Laugel B, Lloyd A, Meermeier EW, Crowther MD, Connor TR, Dolton G, Miles JJ, Burrows SR, Gold MC, Lewinsohn DM, Sewell AK. J Immunol. 2016 Jun 15. pii: 1501402. PubMed
Identifying and retargeting transcriptional hot spots in the human genome. Cheng JK, Lewis AM, Kim DS, Dyess T, Alper HS. Biotechnol J. 2016 Jun 17. doi: 10.1002/biot.201600015. PubMed
CRISPR-Cas9-mediated efficient directed mutagenesis and RAD51-dependent and RAD51-independent gene targeting in the moss Physcomitrella patens. Collonnier C, Epert A, Mara K, Maclot F, Guyon-Debast A, Charlot F, White C, Schaefer DG, Nogue F. Plant Biotechnol J. 2016 Jul 1. doi: 10.1111/pbi.12596. PubMed
Preparation and Analysis of Native Chromatin-Modifying Complexes. Doyon Y, Cote J. Methods Enzymol. 2016;573:303-18. doi: 10.1016/bs.mie.2016.01.017. Epub 2016 Mar 16. PubMed
Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens. Marceau CD, Puschnik AS, Majzoub K, Ooi YS, Brewer SM, Fuchs G, Swaminathan K, Mata MA, Elias JE, Sarnow P, Carette JE. Nature. 2016 Jul 7;535(7610):159-63. doi: 10.1038/nature18631. Epub 2016 Jun 17. PubMed
Loss of LSR affects epithelial barrier integrity and tumor xenograft growth of CaCo-2 cells. Czulkies BA, Mastroianni J, Lutz L, Lang S, Schwan C, Schmidt G, Lassmann S, Zeiser R, Aktories K, Papatheodorou P. Oncotarget. 2017 Jun 6;8(23):37009-37022. doi: 10.18632/oncotarget.10425. PubMed
Microinjection-based generation of mutant mice with a double mutation and a 0.5 Mb deletion in their genome by the CRISPR/Cas9 system. Hara S, Kato T, Goto Y, Kubota S, Tamano M, Terao M, Takada S. J Reprod Dev. 2016 Oct 18;62(5):531-536. doi: 10.1262/jrd.2016-058. Epub 2016 Jul 8. PubMed
ARCN1 Mutations Cause a Recognizable Craniofacial Syndrome Due to COPI-Mediated Transport Defects. Izumi K, Brett M, Nishi E, Drunat S, Tan ES, Fujiki K, Lebon S, Cham B, Masuda K, Arakawa M, Jacquinet A, Yamazumi Y, Chen ST, Verloes A, Okada Y, Katou Y, Nakamura T, Akiyama T, Gressens P, Foo R, Passemard S, Tan EC, El Ghouzzi V, Shirahige K. Am J Hum Genet. 2016 Aug 4;99(2):451-9. doi: 10.1016/j.ajhg.2016.06.011. Epub 2016 Jul 28. PubMed
A Cas9 Variant for Efficient Generation of Indel-Free Knockin or Gene-Corrected Human Pluripotent Stem Cells. Howden SE, McColl B, Glaser A, Vadolas J, Petrou S, Little MH, Elefanty AG, Stanley EG. Stem Cell Reports. 2016 Sep 13;7(3):508-17. doi: 10.1016/j.stemcr.2016.07.001. Epub 2016 Aug 4. PubMed Associated Plasmids pDNR-SpCas9-Gem pDNR-SpCas9 pDNR-SpCas9-Cdt1 pSMART-sgRNA (Sp)
The Dual NOD1/NOD2 Agonism of Muropeptides Containing a Meso-Diaminopimelic Acid Residue. Dagil YA, Arbatsky NP, Alkhazova BI, L'vov VL, Mazurov DV, Pashenkov MV. PLoS One. 2016 Aug 11;11(8):e0160784. doi: 10.1371/journal.pone.0160784. eCollection 2016. PubMed
Application of NanoLuc to monitor the intrinsic promoter activity of GRP78 using the CRISPR/Cas9 system. Oh-Hashi K, Furuta E, Norisada J, Amaya F, Hirata Y, Kiuchi K. Genes Cells. 2016 Oct;21(10):1137-1143. doi: 10.1111/gtc.12401. Epub 2016 Aug 12. PubMed
Human TRAV1-2-negative MR1-restricted T cells detect S. pyogenes and alternatives to MAIT riboflavin-based antigens. Meermeier EW, Laugel BF, Sewell AK, Corbett AJ, Rossjohn J, McCluskey J, Harriff MJ, Franks T, Gold MC, Lewinsohn DM. Nat Commun. 2016 Aug 16;7:12506. doi: 10.1038/ncomms12506. PubMed
Single-cell RNA-seq reveals novel regulators of human embryonic stem cell differentiation to definitive endoderm. Chu LF, Leng N, Zhang J, Hou Z, Mamott D, Vereide DT, Choi J, Kendziorski C, Stewart R, Thomson JA. Genome Biol. 2016 Aug 17;17(1):173. doi: 10.1186/s13059-016-1033-x. PubMed Associated Plasmids T-2A-EGFP-PGK-Puro T-STOP sgRNA-1
CRISPR/Cas9 Targeted Gene Editing and Cellular Engineering in Fanconi Anemia. Osborn M, Lonetree CL, Webber BR, Patel D, Dunmire S, McElroy AN, DeFeo AP, MacMillan ML, Wagner J, Balzar BR, Tolar J. Stem Cells Dev. 2016 Aug 18. PubMed
Analysis of gene repair tracts from Cas9/gRNA double-stranded breaks in the human CFTR gene. Hollywood JA, Lee CM, Scallan MF, Harrison PT. Sci Rep. 2016 Aug 25;6:32230. doi: 10.1038/srep32230. PubMed
Comprehensive protein tyrosine phosphatase mRNA profiling identifies new regulators in the progression of glioma. Bourgonje AM, Verrijp K, Schepens JT, Navis AC, Piepers JA, Palmen CB, van den Eijnden M, Hooft van Huijsduijnen R, Wesseling P, Leenders WP, Hendriks WJ. Acta Neuropathol Commun. 2016 Sep 1;4(1):96. doi: 10.1186/s40478-016-0372-x. PubMed
GAPTrap: A Simple Expression System for Pluripotent Stem Cells and Their Derivatives. Kao T, Labonne T, Niclis JC, Chaurasia R, Lokmic Z, Qian E, Bruveris FF, Howden SE, Motazedian A, Schiesser JV, Costa M, Sourris K, Ng E, Anderson D, Giudice A, Farlie P, Cheung M, Lamande SR, Penington AJ, Parish CL, Thomson LH, Rafii A, Elliott DA, Elefanty AG, Stanley EG. Stem Cell Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 10.1016/j.stemcr.2016.07.015. PubMed Associated Plasmids pGAPTrap-Clover-Muro pGAPTrap-mtagBFP2-IRESMuro pGAPTrap-IRESMuro pGAPTrap-mtagtdTom_IRESMuro pGAPTrap-eGFP-IRESMeo More ...
Augmenting CRISPR applications in Drosophila with tRNA-flanked sgRNAs. Port F, Bullock SL. Nat Methods. 2016 Oct;13(10):852-4. doi: 10.1038/nmeth.3972. Epub 2016 Sep 5. PubMed Associated Plasmids pCFD5 pCFD6 pCFD7 act-AsCpf1
nc886, a non-coding RNA and suppressor of PKR, exerts an oncogenic function in thyroid cancer. Lee EK, Hong SH, Shin S, Lee HS, Lee JS, Park EJ, Choi SS, Min JW, Park D, Hwang JA, Johnson BH, Jeon SH, Kim IH, Lee YS, Lee YS. Oncotarget. 2016 Nov 15;7(46):75000-75012. doi: 10.18632/oncotarget.11852. PubMed
A Library of Infectious Hepatitis C Viruses with Engineered Mutations in the E2 Gene Reveals Growth-Adaptive Mutations That Modulate Interactions with Scavenger Receptor Class B Type I. Zuiani A, Chen K, Schwarz MC, White JP, Luca VC, Fremont DH, Wang D, Evans MJ, Diamond MS. J Virol. 2016 Nov 14;90(23):10499-10512. Print 2016 Dec 1. PubMed
Inheritable Silencing of Endogenous Genes by Hit-and-Run Targeted Epigenetic Editing. Amabile A, Migliara A, Capasso P, Biffi M, Cittaro D, Naldini L, Lombardo A. Cell. 2016 Sep 22;167(1):219-232.e14. doi: 10.1016/j.cell.2016.09.006. PubMed
Sphingosine 1-Phosphate Activation of EGFR As a Novel Target for Meningitic Escherichia coli Penetration of the Blood-Brain Barrier. Wang X, Maruvada R, Morris AJ, Liu JO, Wolfgang MJ, Baek DJ, Bittman R, Kim KS. PLoS Pathog. 2016 Oct 6;12(10):e1005926. doi: 10.1371/journal.ppat.1005926. eCollection 2016 Oct. PubMed
Genomic modelling of the ESR1 Y537S mutation for evaluating function and new therapeutic approaches for metastatic breast cancer. Harrod A, Fulton J, Nguyen VT, Periyasamy M, Ramos-Garcia L, Lai CF, Metodieva G, de Giorgio A, Williams RL, Santos DB, Gomez PJ, Lin ML, Metodiev MV, Stebbing J, Castellano L, Magnani L, Coombes RC, Buluwela L, Ali S. Oncogene. 2016 Oct 17. doi: 10.1038/onc.2016.382. PubMed
A Single Amino Acid Dictates Protein Kinase R Susceptibility to Unrelated Viral Antagonists. Carpentier KS, Esparo NM, Child SJ, Geballe AP. PLoS Pathog. 2016 Oct 25;12(10):e1005966. doi: 10.1371/journal.ppat.1005966. eCollection 2016 Oct. PubMed
The STX6-VTI1B-VAMP3 complex facilitates xenophagy by regulating the fusion between recycling endosomes and autophagosomes. Nozawa T, Minowa-Nozawa A, Aikawa C, Nakagawa I. Autophagy. 2017 Jan 2;13(1):57-69. doi: 10.1080/15548627.2016.1241924. Epub 2016 Oct 28. PubMed
Cas9 in Genetically Modified Food Is Unlikely to Cause Food Allergy. Nakajima O, Nishimaki-Mogami T, Kondo K. Biol Pharm Bull. 2016;39(11):1876-1880. PubMed
CRISPR/Cas9 editing of induced pluripotent stem cells for engineering inflammation-resistant tissues. Brunger JM, Zutshi A, Willard VP, Gersbach CA, Guilak F. Arthritis Rheumatol. 2016 Nov 3. doi: 10.1002/art.39982. PubMed
In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration. Suzuki K, Tsunekawa Y, Hernandez-Benitez R, Wu J, Zhu J, Kim EJ, Hatanaka F, Yamamoto M, Araoka T, Li Z, Kurita M, Hishida T, Li M, Aizawa E, Guo S, Chen S, Goebl A, Soligalla RD, Qu J, Jiang T, Fu X, Jafari M, Esteban CR, Berggren WT, Lajara J, Nunez-Delicado E, Guillen P, Campistol JM, Matsuzaki F, Liu GH, Magistretti P, Zhang K, Callaway EM, Zhang K, Belmonte JC. Nature. 2016 Dec 1;540(7631):144-149. doi: 10.1038/nature20565. Epub 2016 Nov 16. PubMed Associated Plasmids pCAG-1BPNLS-Cas9-1BPNLS pCAG-1BPNLS-Cas9-1BPNLS-2AGFP pCAGmCherry-gRNA Tubb3gRNA-mCherry pTubb3-MC More ...
Glycogen Synthase Kinase 3 (GSK-3)-mediated Phosphorylation of Uracil N-Glycosylase 2 (UNG2) Facilitates the Repair of Floxuridine-induced DNA Lesions and Promotes Cell Survival. Baehr CA, Huntoon CJ, Hoang SM, Jerde CR, Karnitz LM. J Biol Chem. 2016 Dec 23;291(52):26875-26885. doi: 10.1074/jbc.M116.746081. Epub 2016 Nov 14. PubMed
Deletion of conserved sequences in IG-DMR at Dlk1-Gtl2 locus suggests their involvement in expression of paternally expressed genes in mice. Saito T, Hara S, Tamano M, Asahara H, Takada S. J Reprod Dev. 2016 Dec 1. PubMed
Two subunits of human ORC are dispensable for DNA replication and proliferation. Shibata E, Kiran M, Shibata Y, Singh S, Kiran S, Dutta A. Elife. 2016 Dec 1;5. pii: e19084. doi: 10.7554/eLife.19084. PubMed
Tumor Interferon Signaling Regulates a Multigenic Resistance Program to Immune Checkpoint Blockade. Benci JL, Xu B, Qiu Y, Wu TJ, Dada H, Twyman-Saint Victor C, Cucolo L, Lee DS, Pauken KE, Huang AC, Gangadhar TC, Amaravadi RK, Schuchter LM, Feldman MD, Ishwaran H, Vonderheide RH, Maity A, Wherry EJ, Minn AJ. Cell. 2016 Dec 1;167(6):1540-1554.e12. doi: 10.1016/j.cell.2016.11.022. PubMed
Genome-wide screen of cell-cycle regulators in normal and tumor cells identifies a differential response to nucleosome depletion. Sokolova M, Turunen M, Mortusewicz O, Kivioja T, Herr P, Vaharautio A, Bjorklund M, Taipale M, Helleday T, Taipale J. Cell Cycle. 2017 Jan 17;16(2):189-199. doi: 10.1080/15384101.2016.1261765. Epub 2016 Dec 8. PubMed
Knockout of a difficult-to-remove CHO host cell protein, lipoprotein lipase, for improved polysorbate stability in monoclonal antibody formulations. Chiu J, Valente KN, Levy NE, Min L, Lenhoff AM, Lee KH. Biotechnol Bioeng. 2017 May;114(5):1006-1015. doi: 10.1002/bit.26237. Epub 2016 Dec 27. PubMed
Overlapping roles for PARP1 and PARP2 in the recruitment of endogenous XRCC1 and PNKP into oxidized chromatin. Hanzlikova H, Gittens W, Krejcikova K, Zeng Z, Caldecott KW. Nucleic Acids Res. 2016 Dec 13. pii: gkw1246. PubMed
Simultaneous paralogue knockout using a CRISPR-concatemer in mouse small intestinal organoids. Andersson-Rolf A, Merenda A, Mustata RC, Li T, Dietmann S, Koo BK. Dev Biol. 2016 Oct 27. pii: S0012-1606(16)30479-1. doi: 10.1016/j.ydbio.2016.10.016. PubMed Associated Plasmids 2 gRNA concatmer 3 gRNA concatemer 4 gRNA concatemer
XRCC1 mutation is associated with PARP1 hyperactivation and cerebellar ataxia. Hoch NC, Hanzlikova H, Rulten SL, Tetreault M, Komulainen E, Ju L, Hornyak P, Zeng Z, Gittens W, Rey SA, Staras K, Mancini GM, McKinnon PJ, Wang ZQ, Wagner JD, Yoon G, Caldecott KW. Nature. 2017 Jan 5;541(7635):87-91. doi: 10.1038/nature20790. Epub 2016 Dec 21. PubMed
Generation of Hoxc13 knockout pigs recapitulates human ectodermal dysplasia-9. Han K, Liang L, Li L, Ouyang Z, Zhao B, Wang Q, Liu Z, Zhao Y, Ren X, Jiang F, Lai C, Wang K, Yan S, Huang L, Guo L, Zeng K, Lai L, Fan N. Hum Mol Genet. 2016 Dec 22. pii: ddw378. doi: 10.1093/hmg/ddw378. PubMed
Bcl-xL Affects Group A Streptococcus-Induced Autophagy Directly, by Inhibiting Fusion between Autophagosomes and Lysosomes, and Indirectly, by Inhibiting Bacterial Internalization via Interaction with Beclin 1-UVRAG. Nakajima S, Aikawa C, Nozawa T, Minowa-Nozawa A, Toh H, Nakagawa I. PLoS One. 2017 Jan 13;12(1):e0170138. doi: 10.1371/journal.pone.0170138. eCollection 2017. PubMed
RNA Helicase DDX5 Inhibits Reprogramming to Pluripotency by miRNA-Based Repression of RYBP and its PRC1-Dependent and -Independent Functions. Li H, Lai P, Jia J, Song Y, Xia Q, Huang K, He N, Ping W, Chen J, Yang Z, Li J, Yao M, Dong X, Zhao J, Hou C, Esteban MA, Gao S, Pei D, Hutchins AP, Yao H. Cell Stem Cell. 2017 Apr 6;20(4):462-477.e6. doi: 10.1016/j.stem.2016.12.002. Epub 2017 Jan 19. PubMed
A semisynthetic organism engineered for the stable expansion of the genetic alphabet. Zhang Y, Lamb BM, Feldman AW, Zhou AX, Lavergne T, Li L, Romesberg FE. Proc Natl Acad Sci U S A. 2017 Feb 7;114(6):1317-1322. doi: 10.1073/pnas.1616443114. Epub 2017 Jan 23. PubMed
CRISPR/Cas9-Induced (CTGCAG)n Repeat Instability in the Myotonic Dystrophy Type 1 Locus: Implications for Therapeutic Genome Editing. van Agtmaal EL, Andre LM, Willemse M, Cumming SA, van Kessel IDG, van den Broek WJAA, Gourdon G, Furling D, Mouly V, Monckton DG, Wansink DG, Wieringa B. Mol Ther. 2017 Jan 4;25(1):24-43. doi: 10.1016/j.ymthe.2016.10.014. Epub 2017 Jan 4. PubMed
One-step generation of conditional and reversible gene knockouts. Andersson-Rolf A, Mustata RC, Merenda A, Kim J, Perera S, Grego T, Andrews K, Tremble K, Silva JC, Fink J, Skarnes WC, Koo BK. Nat Methods. 2017 Jan 30. doi: 10.1038/nmeth.4156. PubMed Associated Plasmids pUC118-FLIP-Puro pUC118-FLIP-FlpE-Puro pUC118-mCtnnb1-FLIP-Puro pUC118-mCtnnb1-FLIP-FlpE-Puro gRNA-mCtnnb1
Enhancement of single guide RNA transcription for efficient CRISPR/Cas-based genomic engineering. Ui-Tei K, Maruyama S, Nakano Y. Genome. 2017 Jun;60(6):537-545. doi: 10.1139/gen-2016-0127. Epub 2017 Jan 26. PubMed Associated Plasmids pSuperCRISPR-sgRNA-RL69-TCTT pSuperCRISPR-sgRNA-RL69-TTCT pSuperCRISPR-sgRNA-RL69-TTTC pSuperCRISPR-sgRNA-EGFP12-WT(TTTT) pSuperCRISPR-sgRNA-RL864-TTTA More ...
Stage-Specific Human Induced Pluripotent Stem Cells Map the Progression of Myeloid Transformation to Transplantable Leukemia. Kotini AG, Chang CJ, Chow A, Yuan H, Ho TC, Wang T, Vora S, Solovyov A, Husser C, Olszewska M, Teruya-Feldstein J, Perumal D, Klimek VM, Spyridonidis A, Rampal RK, Silverman L, Reddy EP, Papaemmanuil E, Parekh S, Greenbaum BD, Leslie CS, Kharas MG, Papapetrou EP. Cell Stem Cell. 2017 Mar 2;20(3):315-328.e7. doi: 10.1016/j.stem.2017.01.009. Epub 2017 Feb 16. PubMed
A stable but reversible integrated surrogate reporter for assaying CRISPR/Cas9-stimulated homology-directed repair. Wen Y, Liao G, Pritchard T, Zhao TT, Connelly JP, Pruett-Miller SM, Blanc V, Davidson NO, Madison BB. J Biol Chem. 2017 Apr 14;292(15):6148-6162. doi: 10.1074/jbc.M117.777722. Epub 2017 Feb 22. PubMed Associated Plasmids BII-CPspaH2B BII-C3H BII-C3H2B BII-C3HTK BII-B3HTK More ...
ARID2 modulates DNA damage response in human hepatocellular carcinoma cells. Oba A, Shimada S, Akiyama Y, Nishikawaji T, Mogushi K, Ito H, Matsumura S, Aihara A, Mitsunori Y, Ban D, Ochiai T, Kudo A, Asahara H, Kaida A, Miura M, Tanabe M, Tanaka S. J Hepatol. 2017 May;66(5):942-951. doi: 10.1016/j.jhep.2016.12.026. Epub 2017 Feb 24. PubMed
CRISPR/Cas9-based genetic correction for recessive dystrophic epidermolysis bullosa. Webber BR, Osborn MJ, McElroy AN, Twaroski K, Lonetree CL, DeFeo AP, Xia L, Eide C, Lees CJ, McElmurry RT, Riddle MJ, Kim CJ, Patel DD, Blazar BR, Tolar J. NPJ Regen Med. 2016;1. doi: 10.1038/npjregenmed.2016.14. Epub 2016 Dec 8. PubMed
Genetic dissection of colorectal cancer progression by orthotopic transplantation of engineered cancer organoids. Fumagalli A, Drost J, Suijkerbuijk SJ, van Boxtel R, de Ligt J, Offerhaus GJ, Begthel H, Beerling E, Tan EH, Sansom OJ, Cuppen E, Clevers H, van Rheenen J. Proc Natl Acad Sci U S A. 2017 Mar 21;114(12):E2357-E2364. doi: 10.1073/pnas.1701219114. Epub 2017 Mar 7. PubMed
Dual-utility NLS drives RNF169-dependent DNA damage responses. An L, Jiang Y, Ng HH, Man EP, Chen J, Khoo US, Gong Q, Huen MS. Proc Natl Acad Sci U S A. 2017 Apr 4;114(14):E2872-E2881. doi: 10.1073/pnas.1616602114. Epub 2017 Mar 21. PubMed Associated Plasmids pMH-SFB pMH-SFB-USP7 pMH-MYC pMH-Flag-HA pMH-HA More ...
cis-Regulatory Circuits Regulating NEK6 Kinase Overexpression in Transformed B Cells Are Super-Enhancer Independent. Huang Y, Koues OI, Zhao JY, Liu R, Pyfrom SC, Payton JE, Oltz EM. Cell Rep. 2017 Mar 21;18(12):2918-2931. doi: 10.1016/j.celrep.2017.02.067. PubMed
Mechanisms of precise genome editing using oligonucleotide donors. Kan Y, Ruis B, Takasugi T, Hendrickson EA. Genome Res. 2017 Jul;27(7):1099-1111. doi: 10.1101/gr.214775.116. Epub 2017 Mar 29. PubMed
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Identification of the elementary structural units of the DNA damage response. Natale F, Rapp A, Yu W, Maiser A, Harz H, Scholl A, Grulich S, Anton T, Horl D, Chen W, Durante M, Taucher-Scholz G, Leonhardt H, Cardoso MC. Nat Commun. 2017 Jun 12;8:15760. doi: 10.1038/ncomms15760. PubMed
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N-terminal part of Dishevelled DEP domain is required for Wnt/beta-catenin signaling in mammalian cells. Paclikova P, Bernatik O, Radaszkiewicz TW, Bryja V. Mol Cell Biol. 2017 Jul 3. pii: MCB.00145-17. doi: 10.1128/MCB.00145-17. PubMed
Entosis Is Induced by Glucose Starvation. Hamann JC, Surcel A, Chen R, Teragawa C, Albeck JG, Robinson DN, Overholtzer M. Cell Rep. 2017 Jul 5;20(1):201-210. doi: 10.1016/j.celrep.2017.06.037. PubMed
A Role for Mitochondrial Translation in Promotion of Viability in K-Ras Mutant Cells. Martin TD, Cook DR, Choi MY, Li MZ, Haigis KM, Elledge SJ. Cell Rep. 2017 Jul 11;20(2):427-438. doi: 10.1016/j.celrep.2017.06.061. PubMed
Rapidly inducible Cas9 and DSB-ddPCR to probe editing kinetics. Rose JC, Stephany JJ, Valente WJ, Trevillian BM, Dang HV, Bielas JH, Maly DJ, Fowler DM. Nat Methods. 2017 Jul 24. doi: 10.1038/nmeth.4368. PubMed Associated Plasmids ciCas9_pcDNA5 ciCas9(L22)_pcDNA5 ciCas9(F22)_pcDNA5 e-ciCas9_pcDNA5 AAVS1_sgRNA More ...
Ubiquitination of exposed glycoproteins by SCF(FBXO27) directs damaged lysosomes for autophagy. Yoshida Y, Yasuda S, Fujita T, Hamasaki M, Murakami A, Kawawaki J, Iwai K, Saeki Y, Yoshimori T, Matsuda N, Tanaka K. Proc Natl Acad Sci U S A. 2017 Aug 8;114(32):8574-8579. doi: 10.1073/pnas.1702615114. Epub 2017 Jul 25. PubMed
A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer. Merenda A, Andersson-Rolf A, Mustata RC, Li T, Kim H, Koo BK. J Vis Exp. 2017 Jul 12;(125). doi: 10.3791/55916. PubMed
Agonist-induced dimer dissociation as a macromolecular step in G protein-coupled receptor signaling. Petersen J, Wright SC, Rodriguez D, Matricon P, Lahav N, Vromen A, Friedler A, Stromqvist J, Wennmalm S, Carlsson J, Schulte G. Nat Commun. 2017 Aug 9;8(1):226. doi: 10.1038/s41467-017-00253-9. PubMed
CRISPR/Cas9-Mediated Deletion of Foxn1 in NOD/SCID/IL2rg(-/-) Mice Results in Severe Immunodeficiency. Wei X, Lai Y, Li B, Qin L, Xu Y, Lin S, Wang S, Wu Q, Liang Q, Huang G, Deng Q, Liu P, Wu D, Lai L, Yao Y, Li P. Sci Rep. 2017 Aug 10;7(1):7720. doi: 10.1038/s41598-017-08337-8. PubMed
TH2A is phosphorylated at meiotic centromere by Haspin. Hada M, Kim J, Inoue E, Fukuda Y, Tanaka H, Watanabe Y, Okada Y. Chromosoma. 2017 Dec;126(6):769-780. doi: 10.1007/s00412-017-0638-5. Epub 2017 Aug 12. PubMed
Human microcephaly protein RTTN interacts with STIL and is required to build full-length centrioles. Chen HY, Wu CT, Tang CC, Lin YN, Wang WJ, Tang TK. Nat Commun. 2017 Aug 15;8(1):247. doi: 10.1038/s41467-017-00305-0. PubMed
Cell Cycle-Related Kinase (CCRK) regulates ciliogenesis and Hedgehog signaling in mice. Snouffer A, Brown D, Lee H, Walsh J, Lupu F, Norman R, Lechtreck K, Ko HW, Eggenschwiler J. PLoS Genet. 2017 Aug 17;13(8):e1006912. doi: 10.1371/journal.pgen.1006912. eCollection 2017 Aug. PubMed
Functional validation of ATF4 and GADD34 in Neuro2a cells by CRISPR/Cas9-mediated genome editing. Oh-Hashi K, Sugiura N, Amaya F, Isobe KI, Hirata Y. Mol Cell Biochem. 2018 Mar;440(1-2):65-75. doi: 10.1007/s11010-017-3156-0. Epub 2017 Aug 19. PubMed
Rab35 GTPase recruits NDP52 to autophagy targets. Minowa-Nozawa A, Nozawa T, Okamoto-Furuta K, Kohda H, Nakagawa I. EMBO J. 2017 Sep 15;36(18):2790-2807. doi: 10.15252/embj.201796463. Epub 2017 Aug 28. PubMed
The two-pore channel TPC1 is required for efficient protein processing through early and recycling endosomes. Castonguay J, Orth JHC, Muller T, Sleman F, Grimm C, Wahl-Schott C, Biel M, Mallmann RT, Bildl W, Schulte U, Klugbauer N. Sci Rep. 2017 Aug 30;7(1):10038. doi: 10.1038/s41598-017-10607-4. PubMed
Reconstruction of enhancer-target networks in 935 samples of human primary cells, tissues and cell lines. Cao Q, Anyansi C, Hu X, Xu L, Xiong L, Tang W, Mok MTS, Cheng C, Fan X, Gerstein M, Cheng ASL, Yip KY. Nat Genet. 2017 Oct;49(10):1428-1436. doi: 10.1038/ng.3950. Epub 2017 Sep 4. PubMed
Mitochondrial fission facilitates the selective mitophagy of protein aggregates. Burman JL, Pickles S, Wang C, Sekine S, Vargas JNS, Zhang Z, Youle AM, Nezich CL, Wu X, Hammer JA, Youle RJ. J Cell Biol. 2017 Oct 2;216(10):3231-3247. doi: 10.1083/jcb.201612106. Epub 2017 Sep 11. PubMed
BRCA2 suppresses replication stress-induced mitotic and G1 abnormalities through homologous recombination. Feng W, Jasin M. Nat Commun. 2017 Sep 13;8(1):525. doi: 10.1038/s41467-017-00634-0. PubMed
mTOR Controls Mitochondrial Dynamics and Cell Survival via MTFP1. Morita M, Prudent J, Basu K, Goyon V, Katsumura S, Hulea L, Pearl D, Siddiqui N, Strack S, McGuirk S, St-Pierre J, Larsson O, Topisirovic I, Vali H, McBride HM, Bergeron JJ, Sonenberg N. Mol Cell. 2017 Sep 21;67(6):922-935.e5. doi: 10.1016/j.molcel.2017.08.013. Epub 2017 Sep 14. PubMed
Generation of an OCT4 reporter human induced pluripotent stem cell line using CRISPR/SpCas9. Balboa D, Weltner J, Novik Y, Eurola S, Wartiovaara K, Otonkoski T. Stem Cell Res. 2017 Aug;23:105-108. doi: 10.1016/j.scr.2017.07.006. Epub 2017 Jul 11. PubMed Associated Plasmids pUC19-OCT4-T2A-NLS-EmGFP-P2A-Puro CAG-Cas9
A novel requirement for DROSHA in maintenance of mammalian CG methylation. Stathopoulou A, Chhetri JB, Ambrose JC, Esteve PO, Ji L, Erdjument-Bromage H, Zhang G, Neubert TA, Pradhan S, Herrero J, Schmitz RJ, Ooi SKT. Nucleic Acids Res. 2017 Sep 19;45(16):9398-9412. doi: 10.1093/nar/gkx695. PubMed
Application of a novel HiBiT peptide tag for monitoring ATF4 protein expression in Neuro2a cells. Oh-Hashi K, Furuta E, Fujimura K, Hirata Y. Biochem Biophys Rep. 2017 Aug 15;12:40-45. doi: 10.1016/j.bbrep.2017.08.002. eCollection 2017 Dec. PubMed
CRISPR/Cas9-mediated simultaneous knockout of Dmrt1 and Dmrt3 does not recapitulate the 46,XY gonadal dysgenesis observed in 9p24.3 deletion patients. Inui M, Tamano M, Kato T, Takada S. Biochem Biophys Rep. 2017 Jan 9;9:238-244. doi: 10.1016/j.bbrep.2017.01.001. eCollection 2017 Mar. PubMed
p53 controls expression of the DNA deaminase APOBEC3B to limit its potential mutagenic activity in cancer cells. Periyasamy M, Singh AK, Gemma C, Kranjec C, Farzan R, Leach DA, Navaratnam N, Palinkas HL, Vertessy BG, Fenton TR, Doorbar J, Fuller-Pace F, Meek DW, Coombes RC, Buluwela L, Ali S. Nucleic Acids Res. 2017 Nov 2;45(19):11056-11069. doi: 10.1093/nar/gkx721. PubMed
Generation of three miR-122 knockout lines from a human embryonic stem cell line. Liu Y, Wu F, Zhuang Y, Guo D, Abbas N, Wei R, Wang K, Chen Y, Tao J, Wu Y, Yuan F, Pan T, Yang F, Lai K, Lai L, Li YX. Stem Cell Res. 2017 Oct;24:164-168. doi: 10.1016/j.scr.2017.06.004. Epub 2017 Jun 9. PubMed
Generation of two MEN1 knockout lines from a human embryonic stem cell line. Liu Y, Ruzi A, Guo D, Wu F, Liu H, Wu H, Wu Y, Yuan F, Zhuang Y, Yang F, Lai K, Lai L, Li YX. Stem Cell Res. 2017 Oct;24:169-173. doi: 10.1016/j.scr.2017.06.005. Epub 2017 Jun 10. PubMed
Generation of a KCNJ11 homozygous knockout human embryonic stem cell line WAe001-A-12 using CRISPR/Cas9. Yuan F, Guo D, Gao G, Liu Y, Xu Y, Wu Y, Yang F, Ke X, Lai K, Hong L, Li YX. Stem Cell Res. 2017 Oct;24:89-93. doi: 10.1016/j.scr.2017.08.016. Epub 2017 Aug 30. PubMed
Neuroplastin and Basigin Are Essential Auxiliary Subunits of Plasma Membrane Ca(2+)-ATPases and Key Regulators of Ca(2+) Clearance. Schmidt N, Kollewe A, Constantin CE, Henrich S, Ritzau-Jost A, Bildl W, Saalbach A, Hallermann S, Kulik A, Fakler B, Schulte U. Neuron. 2017 Nov 15;96(4):827-838.e9. doi: 10.1016/j.neuron.2017.09.038. Epub 2017 Oct 19. PubMed
Methylation of transcription factor YY2 regulates its transcriptional activity and cell proliferation. Wu XN, Shi TT, He YH, Wang FF, Sang R, Ding JC, Zhang WJ, Shu XY, Shen HF, Yi J, Gao X, Liu W. Cell Discov. 2017 Oct 3;3:17035. doi: 10.1038/celldisc.2017.35. eCollection 2017. PubMed
Methods for Scarless, Selection-Free Generation of Human Cells and Allele-Specific Functional Analysis of Disease-Associated SNPs and Variants of Uncertain Significance. Coggins NB, Stultz J, O'Geen H, Carvajal-Carmona LG, Segal DJ. Sci Rep. 2017 Nov 8;7(1):15044. doi: 10.1038/s41598-017-15407-4. PubMed
DNA methylation defines regional identity of human intestinal epithelial organoids and undergoes dynamic changes during development. Kraiczy J, Nayak KM, Howell KJ, Ross A, Forbester J, Salvestrini C, Mustata R, Perkins S, Andersson-Rolf A, Leenen E, Liebert A, Vallier L, Rosenstiel PC, Stegle O, Dougan G, Heuschkel R, Koo BK, Zilbauer M. Gut. 2019 Jan;68(1):49-61. doi: 10.1136/gutjnl-2017-314817. Epub 2017 Nov 15. PubMed
A Universal Approach to Correct Various HBB Gene Mutations in Human Stem Cells for Gene Therapy of Beta-Thalassemia and Sickle Cell Disease. Cai L, Bai H, Mahairaki V, Gao Y, He C, Wen Y, Jin YC, Wang Y, Pan RL, Qasba A, Ye Z, Cheng L. Stem Cells Transl Med. 2018 Jan;7(1):87-97. doi: 10.1002/sctm.17-0066. Epub 2017 Nov 21. PubMed
Timing of CRISPR/Cas9-related mRNA microinjection after activation as an important factor affecting genome editing efficiency in porcine oocytes. Sato M, Kosuke M, Koriyama M, Inada E, Saitoh I, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S, Miyoshi K. Theriogenology. 2018 Mar 1;108:29-38. doi: 10.1016/j.theriogenology.2017.11.030. Epub 2017 Nov 26. PubMed
Optimized guide RNA structure for genome editing via Cas9. Xu J, Lian W, Jia Y, Li L, Huang Z. Oncotarget. 2017 Oct 7;8(55):94166-94171. doi: 10.18632/oncotarget.21607. eCollection 2017 Nov 7. PubMed
Generation of an ASS1 heterozygous knockout human embryonic stem cell line, WAe001-A-13, using CRISPR/Cas9. Yuan F, Guo D, Liu Y, Xu Y, Gao G, Wu Y, Yang F, Ke X, Lai K, Wei H, Li YX. Stem Cell Res. 2018 Jan;26:67-71. doi: 10.1016/j.scr.2017.11.007. Epub 2017 Nov 10. PubMed
YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration. Yui S, Azzolin L, Maimets M, Pedersen MT, Fordham RP, Hansen SL, Larsen HL, Guiu J, Alves MRP, Rundsten CF, Johansen JV, Li Y, Madsen CD, Nakamura T, Watanabe M, Nielsen OH, Schweiger PJ, Piccolo S, Jensen KB. Cell Stem Cell. 2018 Jan 4;22(1):35-49.e7. doi: 10.1016/j.stem.2017.11.001. Epub 2017 Dec 14. PubMed
Generation of a SMO homozygous knockout human embryonic stem cell line WAe001-A-16 by CRISPR/Cas9 editing. Wu F, Gao G, Pan T, Yang Z, Xu G, Abbas N, Liu Y, Chen Y, Tan S, You K, Ke X, Zhuang Y, Lin X, Yang F, Li YX. Stem Cell Res. 2018 Mar;27:5-9. doi: 10.1016/j.scr.2017.12.002. Epub 2017 Dec 5. PubMed
Human-Specific Adaptations in Vpu Conferring Anti-tetherin Activity Are Critical for Efficient Early HIV-1 Replication In Vivo. Yamada E, Nakaoka S, Klein L, Reith E, Langer S, Hopfensperger K, Iwami S, Schreiber G, Kirchhoff F, Koyanagi Y, Sauter D, Sato K. Cell Host Microbe. 2018 Jan 10;23(1):110-120.e7. doi: 10.1016/j.chom.2017.12.009. PubMed
Functional analysis after rapid degradation of condensins and 3D-EM reveals chromatin volume is uncoupled from chromosome architecture in mitosis. Samejima K, Booth DG, Ogawa H, Paulson JR, Xie L, Watson CA, Platani M, Kanemaki MT, Earnshaw WC. J Cell Sci. 2018 Feb 22;131(4). pii: jcs.210187. doi: 10.1242/jcs.210187. PubMed
T cells specific for a TAP-independent self-peptide remain naive in tumor-bearing mice and are fully exploitable for therapy. Doorduijn EM, Sluijter M, Marijt KA, Querido BJ, van der Burg SH, van Hall T. Oncoimmunology. 2017 Nov 20;7(3):e1382793. doi: 10.1080/2162402X.2017.1382793. eCollection 2018. PubMed
High Efficiency Gene Correction in Hematopoietic Cells by Donor-Template-Free CRISPR/Cas9 Genome Editing. Surun D, Schwable J, Tomasovic A, Ehling R, Stein S, Kurrle N, von Melchner H, Schnutgen F. Mol Ther Nucleic Acids. 2018 Mar 2;10:1-8. doi: 10.1016/j.omtn.2017.11.001. Epub 2017 Nov 10. PubMed
CtIP fusion to Cas9 enhances transgene integration by homology-dependent repair. Charpentier M, Khedher AHY, Menoret S, Brion A, Lamribet K, Dardillac E, Boix C, Perrouault L, Tesson L, Geny S, De Cian A, Itier JM, Anegon I, Lopez B, Giovannangeli C, Concordet JP. Nat Commun. 2018 Mar 19;9(1):1133. doi: 10.1038/s41467-018-03475-7. PubMed Associated Plasmids pCas9-HE pCas9-Geminin pCas9-HE-Geminin pCas9-CtIP
Unexpected binding behaviors of bacterial Argonautes in human cells cast doubts on their use as targetable gene regulators. O'Geen H, Ren C, Coggins NB, Bates SL, Segal DJ. PLoS One. 2018 Mar 27;13(3):e0193818. doi: 10.1371/journal.pone.0193818. eCollection 2018. PubMed Associated Plasmids pCDNA-hTtAgo pCDNA-hNgAgo
Efficient In Vivo Liver-Directed Gene Editing Using CRISPR/Cas9. Singh K, Evens H, Nair N, Rincon MY, Sarcar S, Samara-Kuko E, Chuah MK, VandenDriessche T. Mol Ther. 2018 May 2;26(5):1241-1254. doi: 10.1016/j.ymthe.2018.02.023. Epub 2018 Mar 6. PubMed
Functional Insulator Scanning of CpG Islands to Identify Regulatory Regions of Promoters Using CRISPR. Grob A, Marbiah M, Isalan M. Methods Mol Biol. 2018;1766:285-301. doi: 10.1007/978-1-4939-7768-0_16. PubMed
A Huntingtin Knockin Pig Model Recapitulates Features of Selective Neurodegeneration in Huntington's Disease. Yan S, Tu Z, Liu Z, Fan N, Yang H, Yang S, Yang W, Zhao Y, Ouyang Z, Lai C, Yang H, Li L, Liu Q, Shi H, Xu G, Zhao H, Wei H, Pei Z, Li S, Lai L, Li XJ. Cell. 2018 May 3;173(4):989-1002.e13. doi: 10.1016/j.cell.2018.03.005. Epub 2018 Mar 29. PubMed
JMJD6 Licenses ERalpha-Dependent Enhancer and Coding Gene Activation by Modulating the Recruitment of the CARM1/MED12 Co-activator Complex. Gao WW, Xiao RQ, Zhang WJ, Hu YR, Peng BL, Li WJ, He YH, Shen HF, Ding JC, Huang QX, Ye TY, Li Y, Liu ZY, Ding R, Rosenfeld MG, Liu W. Mol Cell. 2018 Apr 19;70(2):340-357.e8. doi: 10.1016/j.molcel.2018.03.006. Epub 2018 Apr 5. PubMed
Reproductive technologies for the generation and maintenance of valuable animal strains. Kaneko T. J Reprod Dev. 2018 Jun 22;64(3):209-215. doi: 10.1262/jrd.2018-035. Epub 2018 Apr 14. PubMed
Tumor-secreted Pros1 inhibits macrophage M1 polarization to reduce antitumor immune response. Ubil E, Caskey L, Holtzhausen A, Hunter D, Story C, Earp HS. J Clin Invest. 2018 Jun 1;128(6):2356-2369. doi: 10.1172/JCI97354. Epub 2018 Apr 30. PubMed
A genome-wide assessment of conserved SNP alleles reveals a panel of regulatory SNPs relevant to the peripheral nerve. Law WD, Fogarty EA, Vester A, Antonellis A. BMC Genomics. 2018 May 2;19(1):311. doi: 10.1186/s12864-018-4692-z. PubMed
Detection of genome-edited cells by oligoribonucleotide interference-PCR. Fujita T, Yuno M, Kitaura F, Fujii H. DNA Res. 2018 Aug 1;25(4):395-407. doi: 10.1093/dnares/dsy012. PubMed
Targeted Gene Knock Out Using Nuclease-Assisted Vector Integration: Hemi- and Homozygous Deletion of JAG1. Gapinske M, Tague N, Winter J, Underhill GH, Perez-Pinera P. Methods Mol Biol. 2018;1772:233-248. doi: 10.1007/978-1-4939-7795-6_13. PubMed
In Situ Gene Therapy via AAV-CRISPR-Cas9-Mediated Targeted Gene Regulation. Moreno AM, Fu X, Zhu J, Katrekar D, Shih YV, Marlett J, Cabotaje J, Tat J, Naughton J, Lisowski L, Varghese S, Zhang K, Mali P. Mol Ther. 2018 Jul 5;26(7):1818-1827. doi: 10.1016/j.ymthe.2018.04.017. Epub 2018 Apr 25. PubMed
CRISPR/Cas9-mediated specific integration of fat-1 at the goat MSTN locus. Zhang J, Cui ML, Nie YW, Dai B, Li FR, Liu DJ, Liang H, Cang M. FEBS J. 2018 Aug;285(15):2828-2839. doi: 10.1111/febs.14520. Epub 2018 Jun 15. PubMed
Efficiency and Specificity of Targeted Integration Mediated by the Adeno-Associated Virus Serotype 2 Rep 78 Protein. Li P, Marino MP, Zou J, Argaw T, Morreale MT, Iaffaldano BJ, Reiser J. Hum Gene Ther Methods. 2018 Jun;29(3):135-145. doi: 10.1089/hgtb.2018.052. PubMed Associated Plasmids pNL(CMV)Rep78-III/CMV/WPREΔU3 pNL(CMV)Rep78-III/CAG/WPREΔU3
Super-Resolution Imaging Reveals TCTN2 Depletion-Induced IFT88 Lumen Leakage and Ciliary Weakening. Weng RR, Yang TT, Huang CE, Chang CW, Wang WJ, Liao JC. Biophys J. 2018 Jul 17;115(2):263-275. doi: 10.1016/j.bpj.2018.04.051. Epub 2018 Jun 1. PubMed
Application of NanoBiT for Monitoring Dimerization of the Null Hong Kong Variant of alpha-1-Antitrypsin, NHK, in Living Cells. Norisada J, Fujimura K, Amaya F, Kohno H, Hirata Y, Oh-Hashi K. Mol Biotechnol. 2018 Aug;60(8):539-549. doi: 10.1007/s12033-018-0092-5. PubMed
A tandem repeat array in IG-DMR is essential for imprinting of paternal allele at the Dlk1-Dio3 domain during embryonic development. Saito T, Hara S, Kato T, Tamano M, Muramatsu A, Asahara H, Takada S. Hum Mol Genet. 2018 Sep 15;27(18):3283-3292. doi: 10.1093/hmg/ddy235. PubMed
LGP2 virus sensor regulates gene expression network mediated by TRBP-bound microRNAs. Takahashi T, Nakano Y, Onomoto K, Murakami F, Komori C, Suzuki Y, Yoneyama M, Ui-Tei K. Nucleic Acids Res. 2018 Sep 28;46(17):9134-9147. doi: 10.1093/nar/gky575. PubMed Associated Plasmids pcDNA3.1-TRBP-L1+D1+L2-myc/His pcDNA3.1-TRBP-L2+D2+L3-myc/His pcDNA3.1-myc-TRBP-dsRBD1 pcDNA3.1-myc-TRBP-dsRBD2
Identification of an enhancer region within the TP63/LEPREL1 locus containing genetic variants associated with bladder cancer risk. Dudek AM, Vermeulen SH, Kolev D, Grotenhuis AJ, Kiemeney LALM, Verhaegh GW. Cell Oncol (Dordr). 2018 Oct;41(5):555-568. doi: 10.1007/s13402-018-0393-5. Epub 2018 Jun 28. PubMed
Optimized Plasmid Construction Strategy for Cas9. Xu J, Li W, Hossen MM, Jia Y, Li L, Huang Z. Cell Physiol Biochem. 2018;48(1):131-137. doi: 10.1159/000491669. Epub 2018 Jul 12. PubMed
MUNC, an Enhancer RNA Upstream from the MYOD Gene, Induces a Subgroup of Myogenic Transcripts in trans Independently of MyoD. Cichewicz MA, Kiran M, Przanowska RK, Sobierajska E, Shibata Y, Dutta A. Mol Cell Biol. 2018 Sep 28;38(20). pii: MCB.00655-17. doi: 10.1128/MCB.00655-17. Print 2018 Oct 15. PubMed
Species-independent contribution of ZBP1/DAI/DLM-1-triggered necroptosis in host defense against HSV1. Guo H, Gilley RP, Fisher A, Lane R, Landsteiner VJ, Ragan KB, Dovey CM, Carette JE, Upton JW, Mocarski ES, Kaiser WJ. Cell Death Dis. 2018 Jul 26;9(8):816. doi: 10.1038/s41419-018-0868-3. PubMed
Confirming TDP2 mutation in spinocerebellar ataxia autosomal recessive 23 (SCAR23). Zagnoli-Vieira G, Bruni F, Thompson K, He L, Walker S, de Brouwer APM, Taylor R, Niyazov D, Caldecott KW. Neurol Genet. 2018 Aug 1;4(4):e262. doi: 10.1212/NXG.0000000000000262. eCollection 2018 Aug. PubMed
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Advancing the predictivity of skin sensitization by applying a novel HMOX1 reporter system. Zhong G, Li H, Bai J, Pang S, He C, Du X, Wang H, Zhang Q, Xie S, Du H, Dai R, Huang L. Arch Toxicol. 2018 Oct;92(10):3103-3115. doi: 10.1007/s00204-018-2287-8. Epub 2018 Aug 21. PubMed
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Construction of a GLI3 compound heterozygous knockout human embryonic stem cell line WAe001-A-20 by CRISPR/Cas9 editing. Wei R, Yuan F, Wu Y, Liu Y, You K, Yang Z, Chen Y, Getachew A, Wang N, Xu Y, Zhuang Y, Yang F, Li YX. Stem Cell Res. 2018 Oct;32:139-144. doi: 10.1016/j.scr.2018.09.010. Epub 2018 Sep 17. PubMed
Generation of a cancer testis antigen mCherry reporter HCT116 colorectal carcinoma cell line. Chhetri JB, Drousioti E, Guerra-Assuncao JA, Herrero J, Ooi SKT. Heliyon. 2018 Oct 13;4(10):e00858. doi: 10.1016/j.heliyon.2018.e00858. eCollection 2018 Oct. PubMed
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The neurodegenerative diseases ALS and SMA are linked at the molecular level via the ASC-1 complex. Chi B, O'Connell JD, Iocolano AD, Coady JA, Yu Y, Gangopadhyay J, Gygi SP, Reed R. Nucleic Acids Res. 2018 Dec 14;46(22):11939-11951. doi: 10.1093/nar/gky1093. PubMed
LAMTOR2/LAMTOR1 complex is required for TAX1BP1-mediated xenophagy. Lin CY, Nozawa T, Minowa-Nozawa A, Toh H, Aikawa C, Nakagawa I. Cell Microbiol. 2019 Apr;21(4):e12981. doi: 10.1111/cmi.12981. Epub 2019 Jan 21. PubMed
Protocadherin-1 is essential for cell entry by New World hantaviruses. Jangra RK, Herbert AS, Li R, Jae LT, Kleinfelter LM, Slough MM, Barker SL, Guardado-Calvo P, Roman-Sosa G, Dieterle ME, Kuehne AI, Muena NA, Wirchnianski AS, Nyakatura EK, Fels JM, Ng M, Mittler E, Pan J, Bharrhan S, Wec AZ, Lai JR, Sidhu SS, Tischler ND, Rey FA, Moffat J, Brummelkamp TR, Wang Z, Dye JM, Chandran K. Nature. 2018 Nov;563(7732):559-563. doi: 10.1038/s41586-018-0702-1. Epub 2018 Nov 21. PubMed
NLRX1 Negatively Regulates Group A Streptococcus Invasion and Autophagy Induction by Interacting With the Beclin 1-UVRAG Complex. Aikawa C, Nakajima S, Karimine M, Nozawa T, Minowa-Nozawa A, Toh H, Yamada S, Nakagawa I. Front Cell Infect Microbiol. 2018 Nov 14;8:403. doi: 10.3389/fcimb.2018.00403. eCollection 2018. PubMed
NKG2A Blockade Potentiates CD8 T Cell Immunity Induced by Cancer Vaccines. van Montfoort N, Borst L, Korrer MJ, Sluijter M, Marijt KA, Santegoets SJ, van Ham VJ, Ehsan I, Charoentong P, Andre P, Wagtmann N, Welters MJP, Kim YJ, Piersma SJ, van der Burg SH, van Hall T. Cell. 2018 Dec 13;175(7):1744-1755.e15. doi: 10.1016/j.cell.2018.10.028. Epub 2018 Nov 29. PubMed
Mapping of a responsible region for sex reversal upstream of Sox9 by production of mice with serial deletion in a genomic locus. Ogawa Y, Terao M, Hara S, Tamano M, Okayasu H, Kato T, Takada S. Sci Rep. 2018 Nov 30;8(1):17514. doi: 10.1038/s41598-018-35746-0. PubMed
Interleukin 34 (IL-34) cell-surface localization regulated by the molecular chaperone 78-kDa glucose-regulated protein facilitates the differentiation of monocytic cells. Ogawa S, Matsuoka Y, Takada M, Matsui K, Yamane F, Kubota E, Yasuhara S, Hieda K, Kanayama N, Hatano N, Tokumitsu H, Magari M. J Biol Chem. 2019 Feb 15;294(7):2386-2396. doi: 10.1074/jbc.RA118.006226. Epub 2018 Dec 20. PubMed
Homology-independent multiallelic disruption via CRISPR/Cas9-based knock-in yields distinct functional outcomes in human cells. Zhang C, He X, Kwok YK, Wang F, Xue J, Zhao H, Suen KW, Wang CC, Ren J, Chen GG, Lai PBS, Li J, Xia Y, Chan AM, Chan WY, Feng B. BMC Biol. 2018 Dec 28;16(1):151. doi: 10.1186/s12915-018-0616-2. PubMed
Lysosomal Signaling Licenses Embryonic Stem Cell Differentiation via Inactivation of Tfe3. Villegas F, Lehalle D, Mayer D, Rittirsch M, Stadler MB, Zinner M, Olivieri D, Vabres P, Duplomb-Jego L, De Bont ESJM, Duffourd Y, Duijkers F, Avila M, Genevieve D, Houcinat N, Jouan T, Kuentz P, Lichtenbelt KD, Thauvin-Robinet C, St-Onge J, Thevenon J, van Gassen KLI, van Haelst M, van Koningsbruggen S, Hess D, Smallwood SA, Riviere JB, Faivre L, Betschinger J. Cell Stem Cell. 2019 Feb 7;24(2):257-270.e8. doi: 10.1016/j.stem.2018.11.021. Epub 2018 Dec 27. PubMed
Novel Chimeric Gene Therapy Vectors Based on Adeno-Associated Virus and Four Different Mammalian Bocaviruses. Fakhiri J, Schneider MA, Puschhof J, Stanifer M, Schildgen V, Holderbach S, Voss Y, El Andari J, Schildgen O, Boulant S, Meister M, Clevers H, Yan Z, Qiu J, Grimm D. Mol Ther Methods Clin Dev. 2019 Jan 18;12:202-222. doi: 10.1016/j.omtm.2019.01.003. eCollection 2019 Mar 15. PubMed
Reducible branched ester-amine quadpolymers (rBEAQs) co-delivering plasmid DNA and RNA oligonucleotides enable CRISPR/Cas9 genome editing. Rui Y, Wilson D, Sanders K, Green JJ. ACS Appl Mater Interfaces. 2019 Feb 22. doi: 10.1021/acsami.8b20206. PubMed Associated Plasmids PB-CAG-GFPd2
SARM1 deficiency up-regulates XAF1, promotes neuronal apoptosis, and accelerates prion disease. Zhu C, Li B, Frontzek K, Liu Y, Aguzzi A. J Exp Med. 2019 Apr 1;216(4):743-756. doi: 10.1084/jem.20171885. Epub 2019 Mar 6. PubMed
CRISPR-induced RASGAP deficiencies in colorectal cancer organoids reveal that only loss of NF1 promotes resistance to EGFR inhibition. Post JB, Hami N, Mertens AEE, Elfrink S, Bos JL, Snippert HJG. Oncotarget. 2019 Feb 15;10(14):1440-1457. doi: 10.18632/oncotarget.26677. eCollection 2019 Feb 15. PubMed
The Chromatin Structure of CRISPR-Cas9 Target DNA Controls the Balance between Mutagenic and Homology-Directed Gene-Editing Events. Janssen JM, Chen X, Liu J, Goncalves MAFV. Mol Ther Nucleic Acids. 2019 Jun 7;16:141-154. doi: 10.1016/j.omtn.2019.02.009. Epub 2019 Feb 20. PubMed
A critical regulator of Bcl2 revealed by systematic transcript discovery of lncRNAs associated with T-cell differentiation. Saadi W, Kermezli Y, Dao LTM, Mathieu E, Santiago-Algarra D, Manosalva I, Torres M, Belhocine M, Pradel L, Loriod B, Aribi M, Puthier D, Spicuglia S. Sci Rep. 2019 Mar 18;9(1):4707. doi: 10.1038/s41598-019-41247-5. PubMed
TMEPAI/PMEPA1 inhibits Wnt signaling by regulating beta-catenin stability and nuclear accumulation in triple negative breast cancer cells. Amalia R, Abdelaziz M, Puteri MU, Hwang J, Anwar F, Watanabe Y, Kato M. Cell Signal. 2019 Jul;59:24-33. doi: 10.1016/j.cellsig.2019.03.016. Epub 2019 Mar 16. PubMed
Evolution and Comprehensive Analysis of DNaseI Hypersensitive Sites in Regulatory Regions of Primate Brain-Related Genes. Lu Y, Wang X, Yu H, Li J, Jiang Z, Chen B, Lu Y, Wang W, Han C, Ouyang Y, Huang L, Chen C, Tian W, Ling F. Front Genet. 2019 Mar 7;10:152. doi: 10.3389/fgene.2019.00152. eCollection 2019. PubMed
Multiplexed and tunable transcriptional activation by promoter insertion using nuclease-assisted vector integration. Brown A, Winter J, Gapinske M, Tague N, Woods WS, Perez-Pinera P. Nucleic Acids Res. 2019 Jul 9;47(12):e67. doi: 10.1093/nar/gkz210. PubMed
PCNA-Mediated Degradation of p21 Coordinates the DNA Damage Response and Cell Cycle Regulation in Individual Cells. Sheng C, Mendler IH, Rieke S, Snyder P, Jentsch M, Friedrich D, Drossel B, Loewer A. Cell Rep. 2019 Apr 2;27(1):48-58.e7. doi: 10.1016/j.celrep.2019.03.031. PubMed Associated Plasmids pgRNA_CV2
DNA Damage Signaling-Induced Cancer Cell Reprogramming as a Driver of Tumor Relapse. Filipponi D, Emelyanov A, Muller J, Molina C, Nichols J, Bulavin DV. Mol Cell. 2019 May 16;74(4):651-663.e8. doi: 10.1016/j.molcel.2019.03.002. Epub 2019 Apr 3. PubMed
Crumbs3 is a critical factor that regulates invasion and metastasis of colon adenocarcinoma via the specific interaction with FGFR1. Iioka H, Saito K, Sakaguchi M, Tachibana T, Homma K, Kondo E. Int J Cancer. 2019 Apr 13. doi: 10.1002/ijc.32336. PubMed
Comparison of gene editing efficiencies of CRISPR/Cas9 and TALEN for generation of MSTN knock-out cashmere goats. Zhang J, Liu J, Yang W, Cui M, Dai B, Dong Y, Yang J, Zhang X, Liu D, Liang H, Cang M. Theriogenology. 2019 Jul 1;132:1-11. doi: 10.1016/j.theriogenology.2019.03.029. Epub 2019 Apr 1. PubMed
CEP120 interacts with C2CD3 and Talpid3 and is required for centriole appendage assembly and ciliogenesis. Tsai JJ, Hsu WB, Liu JH, Chang CW, Tang TK. Sci Rep. 2019 Apr 15;9(1):6037. doi: 10.1038/s41598-019-42577-0. PubMed
Increasing the specificity of CRISPR systems with engineered RNA secondary structures. Kocak DD, Josephs EA, Bhandarkar V, Adkar SS, Kwon JB, Gersbach CA. Nat Biotechnol. 2019 Jun;37(6):657-666. doi: 10.1038/s41587-019-0095-1. Epub 2019 Apr 15. PubMed
Distinct Requirements for HIV-1 Accessory Proteins during Cell Coculture and Cell-Free Infection. Zotova A, Atemasova A, Pichugin A, Filatov A, Mazurov D. Viruses. 2019 Apr 26;11(5). pii: v11050390. doi: 10.3390/v11050390. PubMed
Genome Editing in Mammalian Cell Lines using CRISPR-Cas. Liu KI, Sutrisnoh NB, Wang Y, Tan MH. J Vis Exp. 2019 Apr 11;(146). doi: 10.3791/59086. PubMed
Targeted deletion of BCL11A gene by CRISPR-Cas9 system for fetal hemoglobin reactivation: A promising approach for gene therapy of beta thalassemia disease. Khosravi MA, Abbasalipour M, Concordet JP, Berg JV, Zeinali S, Arashkia A, Azadmanesh K, Buch T, Karimipoor M. Eur J Pharmacol. 2019 Jul 5;854:398-405. doi: 10.1016/j.ejphar.2019.04.042. Epub 2019 Apr 27. PubMed
Multiplexed promoterless gene expression with CRISPReader. Zhan H, Zhou Q, Gao Q, Li J, Huang W, Liu Y. Genome Biol. 2019 Jun 3;20(1):113. doi: 10.1186/s13059-019-1712-5. PubMed
KLF4 protein stability regulated by interaction with pluripotency transcription factors overrides transcriptional control. Dhaliwal NK, Abatti LE, Mitchell JA. Genes Dev. 2019 Jun 20. pii: gad.324319.119. doi: 10.1101/gad.324319.119. PubMed Associated Plasmids Sox2-t2A-GFP Sox2-t2A-mCherry Klf2-t2A-mCherry CA-Stat3-t2A-mCherry Nanog-t2A-mCherry
HSF2BP Interacts with a Conserved Domain of BRCA2 and Is Required for Mouse Spermatogenesis. Brandsma I, Sato K, van Rossum-Fikkert SE, van Vliet N, Sleddens E, Reuter M, Odijk H, van den Tempel N, Dekkers DHW, Bezstarosti K, Demmers JAA, Maas A, Lebbink J, Wyman C, Essers J, van Gent DC, Baarends WM, Knipscheer P, Kanaar R, Zelensky AN. Cell Rep. 2019 Jun 25;27(13):3790-3798.e7. doi: 10.1016/j.celrep.2019.05.096. PubMed
Adhesion G-protein coupled receptor 56 is required for 3T3-L1 adipogenesis. Al Hasan M, Roy P, Dolan S, Martin PE, Patterson S, Bartholomew C. J Cell Physiol. 2020 Feb;235(2):1601-1614. doi: 10.1002/jcp.29079. Epub 2019 Jul 15. PubMed
Stabilizing heterochromatin by DGCR8 alleviates senescence and osteoarthritis. Deng L, Ren R, Liu Z, Song M, Li J, Wu Z, Ren X, Fu L, Li W, Zhang W, Guillen P, Izpisua Belmonte JC, Chan P, Qu J, Liu GH. Nat Commun. 2019 Jul 26;10(1):3329. doi: 10.1038/s41467-019-10831-8. PubMed
Cholesterol-rich lipid-mediated nanoparticles boost of transfection efficiency, utilized for gene editing by CRISPR-Cas9. Hosseini ES, Nikkhah M, Hosseinkhani S. Int J Nanomedicine. 2019 Jun 11;14:4353-4366. doi: 10.2147/IJN.S199104. eCollection 2019. PubMed
A Cancer-Associated Missense Mutation in PP2A-Aalpha Increases Centrosome Clustering during Mitosis. Antao NV, Marcet-Ortega M, Cifani P, Kentsis A, Foley EA. iScience. 2019 Sep 27;19:74-82. doi: 10.1016/j.isci.2019.07.018. Epub 2019 Jul 17. PubMed
Opposing Functions of Interferon Coordinate Adaptive and Innate Immune Responses to Cancer Immune Checkpoint Blockade. Benci JL, Johnson LR, Choa R, Xu Y, Qiu J, Zhou Z, Xu B, Ye D, Nathanson KL, June CH, Wherry EJ, Zhang NR, Ishwaran H, Hellmann MD, Wolchok JD, Kambayashi T, Minn AJ. Cell. 2019 Aug 8;178(4):933-948.e14. doi: 10.1016/j.cell.2019.07.019. PubMed
Precise in vivo genome editing via single homology arm donor mediated intron-targeting gene integration for genetic disease correction. Suzuki K, Yamamoto M, Hernandez-Benitez R, Li Z, Wei C, Soligalla RD, Aizawa E, Hatanaka F, Kurita M, Reddy P, Ocampo A, Hishida T, Sakurai M, Nemeth AN, Nunez Delicado E, Campistol JM, Magistretti P, Guillen P, Rodriguez Esteban C, Gong J, Yuan Y, Gu Y, Liu GH, Lopez-Otin C, Wu J, Zhang K, Izpisua Belmonte JC. Cell Res. 2019 Oct;29(10):804-819. doi: 10.1038/s41422-019-0213-0. Epub 2019 Aug 23. PubMed
Targeting of early endosomes by autophagy facilitates EGFR recycling and signalling. Fraser J, Simpson J, Fontana R, Kishi-Itakura C, Ktistakis NT, Gammoh N. EMBO Rep. 2019 Oct 4;20(10):e47734. doi: 10.15252/embr.201947734. Epub 2019 Aug 26. PubMed
An In Vitro Human Segmentation Clock Model Derived from Embryonic Stem Cells. Chu LF, Mamott D, Ni Z, Bacher R, Liu C, Swanson S, Kendziorski C, Stewart R, Thomson JA. Cell Rep. 2019 Aug 27;28(9):2247-2255.e5. doi: 10.1016/j.celrep.2019.07.090. PubMed Associated Plasmids HES7-NLuc-2A-tdTomato pCRII-Blunt-U6-HES7-STOP-gRNA pB-TAG-NICD pB-TAG-HES7 pB-EF1a-NLuc-IRES-Puro
Efficient generation of Knock-in/Knock-out marmoset embryo via CRISPR/Cas9 gene editing. Kumita W, Sato K, Suzuki Y, Kurotaki Y, Harada T, Zhou Y, Kishi N, Sato K, Aiba A, Sakakibara Y, Feng G, Okano H, Sasaki E. Sci Rep. 2019 Sep 3;9(1):12719. doi: 10.1038/s41598-019-49110-3. PubMed
Hepatitis C Virus Infection Is Inhibited by a Noncanonical Antiviral Signaling Pathway Targeted by NS3-NS4A. Vazquez C, Tan CY, Horner SM. J Virol. 2019 Nov 13;93(23). pii: JVI.00725-19. doi: 10.1128/JVI.00725-19. Print 2019 Dec 1. PubMed
Deletion of a Seminal Gene Cluster Reinforces a Crucial Role of SVS2 in Male Fertility. Shindo M, Inui M, Kang W, Tamano M, Tingwei C, Takada S, Hibino T, Yoshida M, Yoshida K, Okada H, Iwamoto T, Miyado K, Kawano N. Int J Mol Sci. 2019 Sep 14;20(18). pii: ijms20184557. doi: 10.3390/ijms20184557. PubMed
A new patient-derived iPSC model for dystroglycanopathies validates a compound that increases glycosylation of alpha-dystroglycan. Kim J, Lana B, Torelli S, Ryan D, Catapano F, Ala P, Luft C, Stevens E, Konstantinidis E, Louzada S, Fu B, Paredes-Redondo A, Chan AE, Yang F, Stemple DL, Liu P, Ketteler R, Selwood DL, Muntoni F, Lin YY. EMBO Rep. 2019 Nov 5;20(11):e47967. doi: 10.15252/embr.201947967. Epub 2019 Sep 30. PubMed
Angiogenin generates specific stress-induced tRNA halves and is not involved in tRF-3-mediated gene silencing. Su Z, Kuscu C, Malik A, Shibata E, Dutta A. J Biol Chem. 2019 Nov 8;294(45):16930-16941. doi: 10.1074/jbc.RA119.009272. Epub 2019 Oct 3. PubMed
Coordinated removal of repressive epigenetic modifications during induced reversal of cell identity. Tran KA, Dillingham CM, Sridharan R. EMBO J. 2019 Nov 15;38(22):e101681. doi: 10.15252/embj.2019101681. Epub 2019 Oct 4. PubMed
Functional analysis and development of a CRISPR/Cas9 allelic series for a CPR5 ortholog necessary for proper growth of soybean trichomes. Campbell BW, Hoyle JW, Bucciarelli B, Stec AO, Samac DA, Parrott WA, Stupar RM. Sci Rep. 2019 Oct 14;9(1):14757. doi: 10.1038/s41598-019-51240-7. PubMed
A Maternal Transcription Factor, Junction Mediating and Regulatory Protein is Required for Preimplantation Development in the Mouse. Lin ZL, Li YH, Jin YX, Kim NH. Dev Reprod. 2019 Sep;23(3):285-295. doi: 10.12717/DR.2019.23.3.285. Epub 2019 Sep 30. PubMed
CRISPR/Cas9-mediated gene correction in hemophilia B patient-derived iPSCs. Morishige S, Mizuno S, Ozawa H, Nakamura T, Mazahery A, Nomura K, Seki R, Mouri F, Osaki K, Yamamura K, Okamura T, Nagafuji K. Int J Hematol. 2020 Feb;111(2):225-233. doi: 10.1007/s12185-019-02765-0. Epub 2019 Oct 29. PubMed
LDAF1 and Seipin Form a Lipid Droplet Assembly Complex. Chung J, Wu X, Lambert TJ, Lai ZW, Walther TC, Farese RV Jr. Dev Cell. 2019 Dec 2;51(5):551-563.e7. doi: 10.1016/j.devcel.2019.10.006. Epub 2019 Nov 7. PubMed
Novel gRNA design pipeline to develop broad-spectrum CRISPR/Cas9 gRNAs for safe targeting of the HIV-1 quasispecies in patients. Sullivan NT, Dampier W, Chung CH, Allen AG, Atkins A, Pirrone V, Homan G, Passic S, Williams J, Zhong W, Kercher K, Desimone M, Li L, C Antell G, Mell JC, Ehrlich GD, Szep Z, Jacobson JM, Nonnemacher MR, Wigdahl B. Sci Rep. 2019 Nov 19;9(1):17088. doi: 10.1038/s41598-019-52353-9. PubMed
PODXL1 promotes metastasis of the pancreatic ductal adenocarcinoma by activating the C5aR/C5a axis from the tumor microenvironment. Saito K, Iioka H, Maruyama S, Sumardika IW, Sakaguchi M, Kondo E. Neoplasia. 2019 Dec;21(12):1121-1132. doi: 10.1016/j.neo.2019.09.003. Epub 2019 Nov 20. PubMed
Zfp281 orchestrates interconversion of pluripotent states by engaging Ehmt1 and Zic2. Mayer D, Stadler MB, Rittirsch M, Hess D, Lukonin I, Winzi M, Smith A, Buchholz F, Betschinger J. EMBO J. 2020 Jan 15;39(2):e102591. doi: 10.15252/embj.2019102591. Epub 2019 Nov 29. PubMed
Loss of IL-10 signaling in macrophages limits bacterial killing driven by prostaglandin E2. Mukhopadhyay S, Heinz E, Porreca I, Alasoo K, Yeung A, Yang HT, Schwerd T, Forbester JL, Hale C, Agu CA, Choi YH, Rodrigues J, Capitani M, Jostins-Dean L, Thomas DC, Travis S, Gaffney D, Skarnes WC, Thomson N, Uhlig HH, Dougan G, Powrie F. J Exp Med. 2020 Feb 3;217(2). pii: 132614. doi: 10.1084/jem.20180649. PubMed
Forced isoform switching of Neat1_1 to Neat1_2 leads to the loss of Neat1_1 and the hyperformation of paraspeckles but does not affect the development and growth of mice. Isobe M, Toya H, Mito M, Chiba T, Asahara H, Hirose T, Nakagawa S. RNA. 2020 Mar;26(3):251-264. doi: 10.1261/rna.072587.119. Epub 2019 Dec 10. PubMed
Characterization of IRE1alpha in Neuro2a cells by pharmacological and CRISPR/Cas9 approaches. Oh-Hashi K, Kohno H, Kandeel M, Hirata Y. Mol Cell Biochem. 2020 Feb;465(1-2):53-64. doi: 10.1007/s11010-019-03666-w. Epub 2019 Dec 13. PubMed
Group A Streptococcus NAD-Glycohydrolase Inhibits Caveolin 1-Mediated Internalization Into Human Epithelial Cells. Toh H, Lin CY, Nakajima S, Aikawa C, Nozawa T, Nakagawa I. Front Cell Infect Microbiol. 2019 Nov 28;9:398. doi: 10.3389/fcimb.2019.00398. eCollection 2019. PubMed
NEDD4 and NEDD4L regulate Wnt signalling and intestinal stem cell priming by degrading LGR5 receptor. Novellasdemunt L, Kucharska A, Jamieson C, Prange-Barczynska M, Baulies A, Antas P, van der Vaart J, Gehart H, Maurice MM, Li VS. EMBO J. 2020 Feb 3;39(3):e102771. doi: 10.15252/embj.2019102771. Epub 2019 Dec 23. PubMed
Intra-Vkappa Cluster Recombination Shapes the Ig Kappa Locus Repertoire. Shinoda K, Maman Y, Canela A, Schatz DG, Livak F, Nussenzweig A. Cell Rep. 2019 Dec 24;29(13):4471-4481.e6. doi: 10.1016/j.celrep.2019.11.088. PubMed
Efficient production and transmission of CRISPR/Cas9-mediated mutant alleles at the IG-DMR via generation of mosaic mice using a modified 2CC method. Hara S, Terao M, Muramatsu A, Takada S. Sci Rep. 2019 Dec 27;9(1):20202. doi: 10.1038/s41598-019-56676-5. PubMed
Degradation of the Tumor Suppressor PDCD4 Is Impaired by the Suppression of p62/SQSTM1 and Autophagy. Manirujjaman M, Ozaki I, Murata Y, Guo J, Xia J, Nishioka K, Perveen R, Takahashi H, Anzai K, Matsuhashi S. Cells. 2020 Jan 15;9(1). pii: cells9010218. doi: 10.3390/cells9010218. PubMed
Long-term development of human iPSC-derived pyramidal neurons quantified after transplantation into the neonatal mouse cortex. D'Alessio R, Koukouli F, Blanchard S, Catteau J, Rais C, Lemonnier T, Feraud O, Bennaceur-Griscelli A, Groszer M, Maskos U. Dev Biol. 2020 May 1;461(1):86-95. doi: 10.1016/j.ydbio.2020.01.009. Epub 2020 Jan 23. PubMed
Transcriptional landscape of myogenesis from human pluripotent stem cells reveals a key role of TWIST1 in maintenance of skeletal muscle progenitors. Choi IY, Lim H, Cho HJ, Oh Y, Chou BK, Bai H, Cheng L, Kim YJ, Hyun S, Kim H, Shin JH, Lee G. Elife. 2020 Feb 3;9. pii: 46981. doi: 10.7554/eLife.46981. PubMed
Effects of TDP2/VPg Unlinkase Activity on Picornavirus Infections Downstream of Virus Translation. Holmes AC, Zagnoli-Vieira G, Caldecott KW, Semler BL. Viruses. 2020 Jan 31;12(2). pii: v12020166. doi: 10.3390/v12020166. PubMed
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Expansion of T memory stem cells with superior anti-tumor immunity by Urolithin A-induced mitophagy. Denk D, Petrocelli V, Conche C, Drachsler M, Ziegler PK, Braun A, Kress A, Nicolas AM, Mohs K, Becker C, Neurath MF, Farin HF, Buchholz CJ, Andreux PA, Rinsch C, Greten FR. Immunity. 2022 Nov 8;55(11):2059-2073.e8. doi: 10.1016/j.immuni.2022.09.014. Epub 2022 Oct 19. PubMed
Gelatin-Based Electrospun Nanofibers Cross-Linked Using Horseradish Peroxidase for Plasmid DNA Delivery. Furuno K, Suzuki K, Sakai S. Biomolecules. 2022 Nov 4;12(11):1638. doi: 10.3390/biom12111638. PubMed
Colon tumour cell death causes mTOR dependence by paracrine P2X4 stimulation. Schmitt M, Ceteci F, Gupta J, Pesic M, Bottger TW, Nicolas AM, Kennel KB, Engel E, Schewe M, Callak Kirisozu A, Petrocelli V, Dabiri Y, Varga J, Ramakrishnan M, Karimova M, Ablasser A, Sato T, Arkan MC, de Sauvage FJ, Greten FR. Nature. 2022 Dec;612(7939):347-353. doi: 10.1038/s41586-022-05426-1. Epub 2022 Nov 16. PubMed
Pif1 family helicases promote mutation avoidance during DNA replication. Zhou ZX, Follonier C, Lujan SA, Burkholder AB, Zakian VA, Kunkel TA. Nucleic Acids Res. 2022 Dec 9;50(22):12844-12855. doi: 10.1093/nar/gkac1127. PubMed
High-Performance Delivery of a CRISPR Interference System via Lipid-Polymer Hybrid Nanoparticles Combined with Ultrasound-Mediated Microbubble Destruction for Tumor-Specific Gene Repression. Li Y, Wu P, Zhu M, Liang M, Zhang L, Zong Y, Wan M. Adv Healthc Mater. 2023 Jan 2:e2203082. doi: 10.1002/adhm.202203082. PubMed
Evolution of CRISPR-associated endonucleases as inferred from resurrected proteins. Alonso-Lerma B, Jabalera Y, Samperio S, Morin M, Fernandez A, Hille LT, Silverstein RA, Quesada-Ganuza A, Reifs A, Fernandez-Penalver S, Benitez Y, Soletto L, Gavira JA, Diaz A, Vranken W, Sanchez-Mejias A, Guell M, Mojica FJM, Kleinstiver BP, Moreno-Pelayo MA, Montoliu L, Perez-Jimenez R. Nat Microbiol. 2023 Jan;8(1):77-90. doi: 10.1038/s41564-022-01265-y. Epub 2023 Jan 2. PubMed Associated Plasmids pCMV-T7-NLS(SV40)-anCas(SCA)-BPNLS(SV40)-P2A-EGFP (LTH1378) pCMV-T7-NLS(SV40)-anCas(PDCA)-BPNLS(SV40)-P2A-EGFP (LTH1430) pCMV-T7-NLS(SV40)-anCas(PCA)-BPNLS(SV40)-P2A-EGFP (LTH1441) pCMV-T7-NLS(SV40)-anCas(FCA)-BPNLS(SV40)-P2A-EGFP (LTH1380) pCMV-T7-NLS(SV40)-anCas(BCA)-BPNLS(SV40)-P2A-EGFP (LTH1384) More ...
A universal deep-learning model for zinc finger design enables transcription factor reprogramming. Ichikawa DM, Abdin O, Alerasool N, Kogenaru M, Mueller AL, Wen H, Giganti DO, Goldberg GW, Adams S, Spencer JM, Razavi R, Nim S, Zheng H, Gionco C, Clark FT, Strokach A, Hughes TR, Lionnet T, Taipale M, Kim PM, Noyes MB. Nat Biotechnol. 2023 Jan 26. doi: 10.1038/s41587-022-01624-4. PubMed
Engineered MED12 mutations drive uterine fibroid-like transcriptional and metabolic programs by altering the 3D genome compartmentalization. Buyukcelebi K, Chen X, Abdula F, Duval A, Ozturk H, Seker-Polat F, Jin Q, Yin P, Feng Y, Wei JJ, Bulun S, Yue F, Adli M. Res Sq. 2023 Feb 9:rs.3.rs-2537075. doi: 10.21203/rs.3.rs-2537075/v1. Preprint. PubMed
Integrator complex subunit 15 controls mRNA splicing and is critical for eye development. Azuma N, Yokoi T, Tanaka T, Matsuzaka E, Saida Y, Nishina S, Terao M, Takada S, Fukami M, Okamura K, Maehara K, Yamasaki T, Hirayama J, Nishina H, Handa H, Yamaguchi Y. Hum Mol Genet. 2023 Jun 5;32(12):2032-2045. doi: 10.1093/hmg/ddad034. PubMed
Short tandem repeats are important contributors to silencer elements in T cells. Hussain S, Sadouni N, van Essen D, Dao LTM, Ferre Q, Charbonnier G, Torres M, Gallardo F, Lecellier CH, Sexton T, Saccani S, Spicuglia S. Nucleic Acids Res. 2023 Jun 9;51(10):4845-4866. doi: 10.1093/nar/gkad187. PubMed
Elucidation of OSW-1-Induced Stress Responses in Neuro2a Cells. Oh-Hashi K, Nakamura H, Ogawa H, Hirata Y, Sakurai K. Int J Mol Sci. 2023 Mar 17;24(6):5787. doi: 10.3390/ijms24065787. PubMed
Programmable mammalian translational modulators by CRISPR-associated proteins. Kawasaki S, Ono H, Hirosawa M, Kuwabara T, Sumi S, Lee S, Woltjen K, Saito H. Nat Commun. 2023 Apr 19;14(1):2243. doi: 10.1038/s41467-023-37540-7. PubMed
Identification of the EH CRISPR-Cas9 system on a metagenome and its application to genome engineering. Esquerra-Ruvira B, Baquedano I, Ruiz R, Fernandez A, Montoliu L, Mojica FJM. Microb Biotechnol. 2023 Jul;16(7):1505-1523. doi: 10.1111/1751-7915.14266. Epub 2023 Apr 25. PubMed
USP8 Down-Regulation Promotes Parkin-Independent Mitophagy in the Drosophila Brain and in Human Neurons. Mauri S, Bernardo G, Martinez A, Favaro M, Trevisan M, Cobraiville G, Fillet M, Caicci F, Whitworth AJ, Ziviani E. Cells. 2023 Apr 13;12(8):1143. doi: 10.3390/cells12081143. PubMed
A non-coding variant linked to metabolic obesity with normal weight affects actin remodelling in subcutaneous adipocytes. Glunk V, Laber S, Sinnott-Armstrong N, Sobreira DR, Strobel SM, Batista TM, Kubitz P, Moud BN, Ebert H, Huang Y, Brandl B, Garbo G, Honecker J, Stirling DR, Abdennur N, Calabuig-Navarro V, Skurk T, Ocvirk S, Stemmer K, Cimini BA, Carpenter AE, Dankel SN, Lindgren CM, Hauner H, Nobrega MA, Claussnitzer M. Nat Metab. 2023 May;5(5):861-879. doi: 10.1038/s42255-023-00807-w. Epub 2023 May 30. PubMed
FBH1 deficiency sensitizes cells to WEE1 inhibition by promoting mitotic catastrophe. Jennings L, Walters HA, Mason JM. bioRxiv. 2023 May 18:2023.05.15.540841. doi: 10.1101/2023.05.15.540841. Preprint. PubMed
Engineered MED12 mutations drive leiomyoma-like transcriptional and metabolic programs by altering the 3D genome compartmentalization. Buyukcelebi K, Chen X, Abdula F, Elkafas H, Duval AJ, Ozturk H, Seker-Polat F, Jin Q, Yin P, Feng Y, Bulun SE, Wei JJ, Yue F, Adli M. Nat Commun. 2023 Jul 10;14(1):4057. doi: 10.1038/s41467-023-39684-y. PubMed
Generation of a humanized mesonephros in pigs from induced pluripotent stem cells via embryo complementation. Wang J, Xie W, Li N, Li W, Zhang Z, Fan N, Ouyang Z, Zhao Y, Lai C, Li H, Chen M, Quan L, Li Y, Jiang Y, Jia W, Fu L, Mazid MA, Zhu Y, Maxwell PH, Pan G, Esteban MA, Dai Z, Lai L. Cell Stem Cell. 2023 Sep 7;30(9):1235-1245.e6. doi: 10.1016/j.stem.2023.08.003. PubMed
PCR-Based Strategy for Introducing CRISPR/Cas9 Machinery into Hematopoietic Cell Lines. Gonzalez-Romero E, Martinez-Valiente C, Garcia-Garcia G, Rosal-Vela A, Millan JM, Sanz MA, Sanz G, Liquori A, Cervera JV, Vazquez-Manrique RP. Cancers (Basel). 2023 Aug 25;15(17):4263. doi: 10.3390/cancers15174263. PubMed
Loss of KDM6B epigenetically confers resistance to lipotoxicity in nonalcoholic fatty liver disease-related HCC. Hatano M, Akiyama Y, Shimada S, Yagi K, Akahoshi K, Itoh M, Tanabe M, Ogawa Y, Tanaka S. Hepatol Commun. 2023 Oct 2;7(10):e0277. doi: 10.1097/HC9.0000000000000277. eCollection 2023 Oct 1. PubMed
TDP1 suppresses chromosomal translocations and cell death induced by abortive TOP1 activity during gene transcription. Rubio-Contreras D, Gomez-Herreros F. Nat Commun. 2023 Nov 9;14(1):6940. doi: 10.1038/s41467-023-42622-7. PubMed
Target-dependent RNA polymerase as universal platform for gene expression control in response to intracellular molecules. Komatsu S, Ohno H, Saito H. Nat Commun. 2023 Nov 17;14(1):7256. doi: 10.1038/s41467-023-42802-5. PubMed
CatSper mediates not only chemotactic behavior but also the motility of ascidian sperm. Kijima T, Kurokawa D, Sasakura Y, Ogasawara M, Aratake S, Yoshida K, Yoshida M. Front Cell Dev Biol. 2023 Nov 2;11:1136537. doi: 10.3389/fcell.2023.1136537. eCollection 2023. PubMed
Autophagy supports PDGFRA-dependent brain tumor development by enhancing oncogenic signaling. Simpson JE, Muir MT, Lee M, Naughton C, Gilbert N, Pollard SM, Gammoh N. Dev Cell. 2024 Jan 22;59(2):228-243.e7. doi: 10.1016/j.devcel.2023.11.023. Epub 2023 Dec 18. PubMed

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hCas9 Citations (409)

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